LitCoin-sentences  

PubMed:28148288 JSONTXT 26 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-111 Sentence denotes Metalloprotease-disintegrin ADAM12 actively promotes the stem cell-like phenotype in claudin-low breast cancer.
T2 112-245 Sentence denotes BACKGROUND: ADAM12 is upregulated in human breast cancers and is a predictor of chemoresistance in estrogen receptor-negative tumors.
T3 246-415 Sentence denotes ADAM12 is induced during epithelial-to-mesenchymal transition, a feature associated with claudin-low breast tumors, which are enriched in cancer stem cell (CSC) markers.
T4 416-530 Sentence denotes It is currently unknown whether ADAM12 plays an active role in promoting the CSC phenotype in breast cancer cells.
T5 531-708 Sentence denotes METHODS: ADAM12 expression was downregulated in representative claudin-low breast cancer cell lines, SUM159PT and Hs578T, using siRNA transfection or inducible shRNA expression.
T6 709-1025 Sentence denotes Cell characteristics commonly associated with the CSC phenotype in vitro (cell migration, invasion, anoikis resistance, mammosphere formation, ALDH activity, and expression of the CD44 and CD24 cell surface markers) and in vivo (tumor formation in mice using limiting dilution transplantation assays) were evaluated.
T7 1026-1121 Sentence denotes RNA sequencing was performed to identify global gene expression changes after ADAM12 knockdown.
T8 1122-1130 Sentence denotes RESULTS:
T9 1131-1287 Sentence denotes We found that sorted SUM159PT cell populations with high ADAM12 levels had elevated expression of CSC markers and an increased ability to form mammospheres.
T10 1288-1410 Sentence denotes ADAM12 knockdown reduced cell migration and invasion, decreased anoikis resistance, and compromised mammosphere formation.
T11 1411-1557 Sentence denotes ADAM12 knockdown also diminished ALDEFLUOR(+) and CD44(hi)/CD24(-/lo) CSC-enriched populations in vitro and reduced tumorigenesis in mice in vivo.
T12 1558-1682 Sentence denotes RNA sequencing identified a significant overlap between ADAM12- and Epidermal Growth Factor Receptor (EGFR)-regulated genes.
T13 1683-1833 Sentence denotes Consequently, ADAM12 knockdown lowered the basal activation level of EGFR, and this effect was abolished by batimastat, a metalloproteinase inhibitor.
T14 1834-1982 Sentence denotes Furthermore, incubation of cells with exogenously added EGF prevented the downregulation of CD44(hi)/CD24(-/lo) cell population by ADAM12 knockdown.
T15 1983-1995 Sentence denotes CONCLUSIONS:
T16 1996-2137 Sentence denotes These results indicate that ADAM12 actively supports the CSC phenotype in claudin-low breast cancer cells via modulation of the EGFR pathway.