LitCoin-sentences  

PubMed:27464336 JSONTXT 25 Projects

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Id Subject Object Predicate Lexical cue
T1 0-194 Sentence denotes Decreased Whole-Body Fat Mass Produced by Chronic Alcohol Consumption is Associated with Activation of S6K1-Mediated Protein Synthesis and Increased Autophagy in Epididymal White Adipose Tissue.
T2 195-206 Sentence denotes BACKGROUND:
T3 207-359 Sentence denotes Chronic alcohol consumption leads to a loss of white adipose tissue (WAT) but the underlying mechanisms for this lipodystrophy are not fully elucidated.
T4 360-583 Sentence denotes This study tested the hypothesis that the reduction in WAT mass in chronic alcohol-fed mice is associated with a decreased protein synthesis specifically related to impaired function of mammalian target of rapamycin (mTOR).
T5 584-592 Sentence denotes METHODS:
T6 593-715 Sentence denotes Adult male mice were provided an alcohol-containing liquid diet for 24 weeks or an isonitrogenous isocaloric control diet.
T7 716-920 Sentence denotes In vivo protein synthesis was determined at this time and thereafter epididymal WAT (eWAT) was excised for analysis of signal transduction pathways central to controling protein synthesis and degradation.
T8 921-929 Sentence denotes RESULTS:
T9 930-1072 Sentence denotes While chronic alcohol feeding decreased whole-body and eWAT mass, this was associated with a discordant increase in protein synthesis in eWAT.
T10 1073-1168 Sentence denotes This increase was not associated with a change in mTOR, 4E-BP1, Akt, or PRAS40 phosphorylation.
T11 1169-1303 Sentence denotes Instead, a selective increase in phosphorylation of S6K1 and its downstream substrates, S6 and eIF4B was detected in alcohol-fed mice.
T12 1304-1433 Sentence denotes Alcohol also increased eEF2K phosphorylation and decreased eEF2 phosphorylation consistent with increased translation elongation.
T13 1434-1619 Sentence denotes Alcohol increased Atg12-5, LC3B-I and -II, and ULK1 S555 phosphorylation, suggesting increased autophagy, while markers of apoptosis (cleaved caspase-3 and -9, and PARP) were unchanged.
T14 1620-1762 Sentence denotes Lipolytic enzymes (ATGL and HSL phosphorylation) were increased and lipogenic regulators (PPARγ and C/EBPα) were decreased in eWAT by alcohol.
T15 1763-1969 Sentence denotes Although alcohol increased TNF-α, IL-6, and IL-1β mRNA, no change in key components of the NLRP3 inflammasome (NLRP3, ACS, and cleaved caspase-1) was detected suggesting alcohol did not increase pyroptosis.
T16 1970-2015 Sentence denotes Plasma insulin did not differ between groups.
T17 2016-2028 Sentence denotes CONCLUSIONS:
T18 2029-2263 Sentence denotes These results demonstrate that the alcohol-induced decrease in whole-body fat mass resulted in part from activation of autophagy in eWAT as protein synthesis was increased and mediated by the specific increase in the activity of S6K1.