LitCoin-sentences  

PubMed:27184800 JSONTXT 26 Projects

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Id Subject Object Predicate Lexical cue
T1 0-116 Sentence denotes Metallothionein-1 as a biomarker of altered redox metabolism in hepatocellular carcinoma cells exposed to sorafenib.
T2 117-128 Sentence denotes BACKGROUND:
T3 129-294 Sentence denotes Sorafenib, a kinase inhibitor active against various solid tumours, induces oxidative stress and ferroptosis, a new form of oxidative necrosis, in some cancer cells.
T4 295-417 Sentence denotes Clinically-applicable biomarkers that reflect the impact of sorafenib on the redox metabolism of cancer cells are lacking.
T5 418-426 Sentence denotes METHODS:
T6 427-637 Sentence denotes We used gene expression microarrays, real-time PCR, immunoblot, protein-specific ELISA, and gene reporter constructs encoding the enzyme luciferase to study the response of a panel of cancer cells to sorafenib.
T7 638-796 Sentence denotes Tumour explants prepared from surgical hepatocellular carcinoma (HCC) samples and serum samples obtained from HCC patients receiving sorafenib were also used.
T8 797-805 Sentence denotes RESULTS:
T9 806-926 Sentence denotes We observed that genes of the metallothionein-1 (MT1) family are induced in the HCC cell line Huh7 exposed to sorafenib.
T10 927-1096 Sentence denotes Sorafenib increased the expression of MT1G mRNA in a panel of human cancer cells, an effect that was not observed with eight other clinically-approved kinase inhibitors.
T11 1097-1375 Sentence denotes We identified the minimal region of the MT1G promoter that confers inducibility by sorafenib to a 133 base pair region containing an Anti-oxidant Response Element (ARE) and showed the essential role of the transcription factor NRF2 (Nuclear factor erythroid 2-Related Factor 2).
T12 1376-1522 Sentence denotes We examined the clinical relevance of our findings by analysing the regulation of MT1G in five tumour explants prepared from surgical HCC samples.
T13 1523-1692 Sentence denotes Finally, we showed that the protein levels of MT1 increase in the serum of some HCC patients receiving sorafenib, and found an association with reduced overall survival.
T14 1693-1704 Sentence denotes CONCLUSION:
T15 1705-1847 Sentence denotes These findings indicate that MT1 constitute a biomarker adapted for exploring the impact of sorafenib on the redox metabolism of cancer cells.