LitCoin-sentences  

PubMed:26847345 JSONTXT 26 Projects

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Id Subject Object Predicate Lexical cue
T1 0-134 Sentence denotes Transgelin increases metastatic potential of colorectal cancer cells in vivo and alters expression of genes involved in cell motility.
T2 135-146 Sentence denotes BACKGROUND:
T3 147-225 Sentence denotes Transgelin is an actin-binding protein that promotes motility in normal cells.
T4 226-415 Sentence denotes Although the role of transgelin in cancer is controversial, a number of studies have shown that elevated levels correlate with aggressive tumor behavior, advanced stage, and poor prognosis.
T5 416-631 Sentence denotes Here we sought to determine the role of transgelin more directly by determining whether experimental manipulation of transgelin levels in colorectal cancer (CRC) cells led to changes in metastatic potential in vivo.
T6 632-640 Sentence denotes METHODS:
T7 641-825 Sentence denotes Isogenic CRC cell lines that differ in transgelin expression were characterized using in vitro assays of growth and invasiveness and a mouse tail vein assay of experimental metastasis.
T8 826-942 Sentence denotes Downstream effects of transgelin overexpression were investigated by gene expression profiling and quantitative PCR.
T9 943-951 Sentence denotes RESULTS:
T10 952-1114 Sentence denotes Stable overexpression of transgelin in RKO cells, which have low endogenous levels, led to increased invasiveness, growth at low density, and growth in soft agar.
T11 1115-1235 Sentence denotes Overexpression also led to an increase in the number and size of lung metastases in the mouse tail vein injection model.
T12 1236-1375 Sentence denotes Similarly, attenuation of transgelin expression in HCT116 cells, which have high endogenous levels, decreased metastases in the same model.
T13 1376-1610 Sentence denotes Investigation of mRNA expression patterns showed that transgelin overexpression altered the levels of approximately 250 other transcripts, with over-representation of genes that affect function of actin or other cytoskeletal proteins.
T14 1611-1717 Sentence denotes Changes included increases in HOOK1, SDCCAG8, ENAH/Mena, and TNS1 and decreases in EMB, BCL11B, and PTPRD.
T15 1718-1730 Sentence denotes CONCLUSIONS:
T16 1731-1867 Sentence denotes Increases or decreases in transgelin levels have reciprocal effects on tumor cell behavior, with higher expression promoting metastasis.
T17 1868-1960 Sentence denotes Chronic overexpression influences steady-state levels of mRNAs for metastasis-related genes.