LitCoin-sentences  

PubMed:24623966 JSONTXT 31 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-168 Sentence denotes Endothelial NADPH oxidase 4 mediates vascular endothelial growth factor receptor 2-induced intravitreal neovascularization in a rat model of retinopathy of prematurity.
T2 169-263 Sentence denotes PURPOSE: NADPH oxidase-generated reactive oxygen species (ROS) are implicated in angiogenesis.
T3 264-367 Sentence denotes Isoforms of NADPH oxidase NOX1, NOX2, and NOX4 are reported to be expressed in endothelial cells (ECs).
T4 368-510 Sentence denotes Of these, NOX1 and NOX2 have been reported to contribute to intravitreal neovascularization (IVNV) in oxygen-induced retinopathy (OIR) models.
T5 511-667 Sentence denotes In this study, we tested the hypothesis that the isoform NOX4 in ECs contributed to vascular endothelial growth factor (VEGF)-induced angiogenesis and IVNV.
T6 668-676 Sentence denotes METHODS:
T7 677-892 Sentence denotes Isoforms of NADPH oxidase MRNA were measured in several types of cultured vascular ecs: human retinal microvascular ECs (hRMVECs), choroidal ECs (CECs), and human umbilical vascular ECs (HUVECs) using real-time PCR.
T8 893-1105 Sentence denotes Newborn rat pups and dams were placed into an OIR model that cycled oxygen concentration between 50% and 10% every 24 h for 14 days, and then were placed in room air (RA) for an additional 4 days (rat OIR model).
T9 1106-1237 Sentence denotes NOX4 expression in retinal lysates from the RA-raised pups at postnatal day 0 (P0), P14, and P18 was determined with western blots.
T10 1238-1424 Sentence denotes STAT3 activation was determined as the ratio of phosphorylated STAT3 to total STAT3 with western blot analysis of retinal lysates from pups raised in RA or from the rat OIR model at P18.
T11 1425-1606 Sentence denotes Semiquantitative assessment of the density of NOX4 colabeling with lectin-stained retinal ECs was determined by immunolabeling of retinal cryosections from P18 pups in OIR or in RA.
T12 1607-1935 Sentence denotes In hRMVECs transfected with NOX4 siRNA and treated with VEGF or control, 1) ROS generation was measured using the 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl ester fluorescence assay and 2) phosphorylated VEGF receptor 2 and STAT3, and total VEGFR2 and STAT3 were measured in western blot analyses.
T13 1936-2064 Sentence denotes VEGF-stimulated hRMVEC proliferation was measured following transfection with NOX4 siRNA or STAT3 siRNA, or respective controls.
T14 2065-2143 Sentence denotes RESULTS: NOX4 was the most prevalent isoform of NADPH oxidase in vascular ECs.
T15 2144-2232 Sentence denotes NOX4 expression in retinal lysates was significantly decreased during development in RA.
T16 2233-2297 Sentence denotes Compared to RA, the expression of retinal NOX4 increased at P18.
T17 2298-2530 Sentence denotes At p18 OIR, semiquantitative assessment of the density of lectin and NOX4 colabeling in retinal vascular ECs was greater in retinal cryosections and activated STAT3 was greater in retinal lysates when compared to the RA-raised pups.
T18 2531-2630 Sentence denotes In cultured hRMVECs, knockdown of NOX4 by siRNA transfection inhibited VEGF-induced ROS generation.
T19 2631-2701 Sentence denotes VEGF induced a physical interaction of phosphorylated-VEGFR2 and NOX4.
T20 2702-2720 Sentence denotes Knockdown of NOX4:
T21 2721-2827 Sentence denotes 1) reduced VEGFR2 activation but did not abolish it and 2) abolished STAT3 activation in response to VEGF.
T22 2828-2902 Sentence denotes Knockdown of either NOX4 or STAT3 inhibited VEGF-induced EC proliferation.
T23 2903-2915 Sentence denotes CONCLUSIONS:
T24 2916-3056 Sentence denotes Our data suggest that in a model representative of human retinopathy of prematurity, NOX4 was increased at a time point when IVNV developed.
T25 3057-3193 Sentence denotes VEGF-activated NOX4 led to an interaction between VEGF-activated VEGFR2 and NOX4 that mediated EC proliferation via activation of STAT3.
T26 3194-3294 Sentence denotes Altogether, our results suggest that NOX4 may regulate VEGFR2-mediated IVNV through activated STAT3.