LitCoin-sentences  

PubMed:21750150 JSONTXT 27 Projects

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Id Subject Object Predicate Lexical cue
T1 0-84 Sentence denotes Homozygously deleted gene DACH1 regulates tumor-initiating activity of glioma cells.
T2 85-170 Sentence denotes Loss or reduction in function of tumor suppressor genes contributes to tumorigenesis.
T3 171-397 Sentence denotes Here, by allelic DNA copy number analysis using single-nucleotide polymorphism genotyping array and mass spectrometry, we report homozygous deletion in glioblastoma multiformes at chromosome 13q21, where DACH1 gene is located.
T4 398-499 Sentence denotes We found decreased cell proliferation of a series of glioma cell lines by forced expression of DACH1.
T5 500-623 Sentence denotes We then generated U87TR-Da glioma cells, where DACH1 expression could be activated by exposure of the cells to doxycycline.
T6 624-850 Sentence denotes Both ex vivo cellular proliferation and in vivo growth of s.c. transplanted tumors in mice are reduced in U87TR-Da cells with DACH1 expression (U87-DACH1-high), compared with DACH1-nonexpressing U87TR-Da cells (U87-DACH1-low).
T7 851-972 Sentence denotes U87-DACH1-low cells form spheroids with CD133 and Nestin expression in serum-free medium but U87-DACH1-high cells do not.
T8 973-1149 Sentence denotes Compared with spheroid-forming U87-DACH1-low cells, adherent U87-DACH1-high cells display lower tumorigenicity, indicating DACH1 decreases the number of tumor-initiating cells.
T9 1150-1357 Sentence denotes Gene expression analysis and chromatin immunoprecipitation assay reveal that fibroblast growth factor 2 (FGF2/bFGF) is transcriptionally repressed by DACH1, especially in cells cultured in serum-free medium.
T10 1358-1575 Sentence denotes Exogenous bFGF rescues spheroid-forming activity and tumorigenicity of the U87-DACH1-high cells, suggesting that loss of DACH1 increases the number of tumor-initiating cells through transcriptional activation of bFGF.
T11 1576-1773 Sentence denotes These results illustrate that DACH1 is a distinctive tumor suppressor, which does not only suppress growth of tumor cells but also regulates bFGF-mediated tumor-initiating activity of glioma cells.