| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-227 |
Sentence |
denotes |
Promoter insertion/deletion in the IRF5 gene is highly associated with susceptibility to systemic lupus erythematosus in distinct populations, but exerts a modest effect on gene expression in peripheral blood mononuclear cells. |
| T2 |
228-238 |
Sentence |
denotes |
OBJECTIVE: |
| T3 |
239-436 |
Sentence |
denotes |
We examined the genetic association of the promoter insertion/deletion (indel) in IRF5 gene with systemic lupus erythematosus (SLE) in distinct populations and assessed its role in gene expression. |
| T4 |
437-445 |
Sentence |
denotes |
METHODS: |
| T5 |
446-524 |
Sentence |
denotes |
Four IRF5 polymorphisms were genotyped in 1488 SLE patients and 1466 controls. |
| T6 |
525-641 |
Sentence |
denotes |
Gene expression was analyzed by quantitative real-time PCR using RNA from peripheral blood mononuclear cells (PBMC). |
| T7 |
642-650 |
Sentence |
denotes |
RESULTS: |
| T8 |
651-781 |
Sentence |
denotes |
The promoter indel and rs2070197 had independent genetic effects, which accounted for the association of rs2004640 and rs10954213. |
| T9 |
782-889 |
Sentence |
denotes |
Gene expression analysis revealed that rs10954213 exerted the greatest influence on IRF5 transcript levels. |
| T10 |
890-901 |
Sentence |
denotes |
CONCLUSION: |
| T11 |
902-1112 |
Sentence |
denotes |
We corroborated the association of the promoter indel with SLE in 5 different populations and revealed that rs10954213 is the main single-nucleotide polymorphism responsible for altered IRF5 expression in PBMC. |
