LitCoin-sentences  

PubMed:17879945 JSONTXT 24 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-112 Sentence denotes Estrogen prevents cholesteryl ester accumulation in macrophages induced by the HIV protease inhibitor ritonavir.
T2 113-234 Sentence denotes Individuals with HIV can now live long lives with drug therapy that often includes protease inhibitors such as ritonavir.
T3 235-333 Sentence denotes Many patients, however, develop negative long-term side effects such as premature atherosclerosis.
T4 334-487 Sentence denotes We have previously demonstrated that ritonavir treatment increases atherosclerotic lesion formation in male mice to a greater extent than in female mice.
T5 488-608 Sentence denotes Furthermore, peripheral blood monocytes isolated from ritonavir-treated females had less cholesteryl ester accumulation.
T6 609-802 Sentence denotes In the present study, we have investigated the molecular mechanisms by which female hormones influence cholesterol metabolism in macrophages in response to the HIV protease inhibitor ritonavir.
T7 803-892 Sentence denotes We have utilized the human monocyte cell line, THP-1 as a model to address this question.
T8 893-1096 Sentence denotes Briefly, cells were differentiated for 72 h with 100 nM PMA to obtain a macrophage-like phenotype in the presence or absence of 1 nM 17beta-estradiol (E2), 100 nM progesterone or vehicle (0.01% ethanol).
T9 1097-1199 Sentence denotes Cells were then treated with 30 ng/ml ritonavir or vehicle in the presence of aggregated LDL for 24 h.
T10 1200-1266 Sentence denotes Cell extracts were harvested, and lipid or total RNA was isolated.
T11 1267-1364 Sentence denotes E2 decreased the accumulation of cholesteryl esters in macrophages following ritonavir treatment.
T12 1365-1472 Sentence denotes Ritonavir increased the expression of the scavenger receptor, CD36 mRNA, responsible for the uptake of LDL.
T13 1473-1646 Sentence denotes Additionally, ritonavir treatment selectively increased the relative levels of PPARgamma mRNA, a transcription factor responsible for the regulation of CD36 mRNA expression.
T14 1647-1727 Sentence denotes Treatment with E2, however, failed to prevent these increases at the mRNA level.
T15 1728-1835 Sentence denotes E2 did, however, significantly suppress CD36 protein levels as measured by fluorescent immunocytochemistry.
T16 1836-2044 Sentence denotes This data suggests that E2 modifies the expression of CD36 at the level of protein expression in monocyte-derived macrophages resulting in reduced cholesteryl ester accumulation following ritonavir treatment.