| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-138 |
Sentence |
denotes |
Mutations in pattern recognition receptor genes modulate seroreactivity to microbial antigens in patients with inflammatory bowel disease. |
| T2 |
139-159 |
Sentence |
denotes |
BACKGROUND AND AIMS: |
| T3 |
160-269 |
Sentence |
denotes |
A number of antibodies against microbial epitopes or self-antigens have been associated with Crohn's disease. |
| T4 |
270-455 |
Sentence |
denotes |
The development of antibodies reflects a loss of tolerance to intestinal bacteria that underlies Crohn's disease, resulting in an exaggerated adaptive immune response to these bacteria. |
| T5 |
456-613 |
Sentence |
denotes |
It was hypothesised that the development of antimicrobial antibodies is influenced by the presence of genetic variants in pattern recognition receptor genes. |
| T6 |
614-992 |
Sentence |
denotes |
The aim of this study was therefore to investigate the influence of mutations in these innate immune receptor genes (nucleotide oligomerisation domain (NOD) 2/caspase recruitment domain (CARD) 15, NOD1/CARD4, TUCAN/CARDINAL/CARD8, Toll-like receptor (TLR) 4, TLR2, TLR1 and TLR6) on the development of antimicrobial and antiglycan antibodies in inflammatory bowel disease (IBD). |
| T7 |
993-1015 |
Sentence |
denotes |
MATERIALS AND METHODS: |
| T8 |
1016-1490 |
Sentence |
denotes |
A cohort of 1163 unrelated patients with IBD (874 Crohn's disease, 259 ulcerative colitis, 30 indeterminate colitis) and 312 controls were analysed for anti-Saccharomyces cerevisiae antibodies (gASCA) IgG, anti-laminaribioside antibodies (ALCA) IgG, anti-chitobioside antibodies (ACCA) IgA, anti-mannobioside antibodies (AMCA) IgG and outer membrane porin (Omp) IgA and were genotyped for variants in NOD2/CARD15, TUCAN/CARDINAL/CARD8, NOD1/CARD4, TLR4, TLR1, TLR2 and TLR6. |
| T9 |
1491-1499 |
Sentence |
denotes |
RESULTS: |
| T10 |
1500-1868 |
Sentence |
denotes |
When compared with Crohn's disease patients without CARD15 mutations, the presence of at least one CARD15 variant in Crohn's disease patients more frequently led to gASCA positivity (66.1% versus 51.5%, p < 0.0001) and ALCA positivity (43.3% versus 34.9%, p = 0.018) and higher gASCA titers (85.7 versus 51.8 ELISA units, p < 0.0001), independent of ileal involvement. |
| T11 |
1869-2030 |
Sentence |
denotes |
A gene dosage effect, with increasing gASCA and ALCA positivity for patients carrying none, one and two CARD15 variants, respectively, was seen for both markers. |
| T12 |
2031-2225 |
Sentence |
denotes |
Similarly, Crohn's disease patients carrying NOD1/CARD4 indel had a higher prevalence of gASCA antibodies than wild-type patients (63.8% versus 55.2%, p = 0.014), also with a gene dosage effect. |
| T13 |
2226-2440 |
Sentence |
denotes |
An opposite effect was observed for the TLR4 D299G and TLR2 P631H variants, with a lower prevalence of ACCA antibodies (23.4% versus 35%, p = 0.013) and Omp antibodies (20.5% versus 34.6%, p = 0.009), respectively. |
| T14 |
2441-2452 |
Sentence |
denotes |
CONCLUSION: |
| T15 |
2453-2564 |
Sentence |
denotes |
Variants in innate immune receptor genes were found to influence antibody formation against microbial epitopes. |
| T16 |
2565-2664 |
Sentence |
denotes |
In this respect, it is intriguing that an opposite effect of CARD15 and TLR4 variants was observed. |
| T17 |
2665-2770 |
Sentence |
denotes |
These findings may contribute to an understanding of the aetiology of the seroreactivity observed in IBD. |
