LitCoin-sentences  

PubMed:16288199 JSONTXT 25 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-124 Sentence denotes The promoter of inducible nitric oxide synthase implicated in glaucoma based on genetic analysis and nuclear factor binding.
T2 125-133 Sentence denotes PURPOSE:
T3 134-390 Sentence denotes Nitric oxide has many beneficial functions in the human body at the right amounts, but it can also be hazardous if it is produced in amounts more than needed and has therefore been studied in relation to several neurological and non-neurological disorders.
T4 391-599 Sentence denotes In vitro and in vivo studies demonstrate a connection between the inducible form of Nitric Oxide Synthase, iNOS, and the neuropathological disorder glaucoma, one of the major causes of blindness in the world.
T5 600-799 Sentence denotes In this study, we sought to establish the genetic association between iNOS and primary open angle glaucoma, POAG, and to find the functional element(s) connected with the pathogenesis of the disease.
T6 800-808 Sentence denotes METHODS:
T7 809-995 Sentence denotes Two microsatellites, 1 insertion/deletion, and 8 single nucleotide polymorphisms (SNPs) in the regulatory region of iNOS were genotyped in 200 POAG patients and 200 age-matched controls.
T8 996-1124 Sentence denotes Also, the CCTTT-microsatellite was examined for its protein-binding capability in an electrophoretic mobility shift assay, EMSA.
T9 1125-1133 Sentence denotes RESULTS:
T10 1134-1435 Sentence denotes There was a significant difference in allele distribution of the CCTTT-microsatellite, between patients and controls. (CCTTT)14, which has been reported to have a higher activity in a reporter-construct, was significantly more abundant in POAG patients, while (CCTTT)10 and (CCTTT)13 were less common.
T11 1436-1513 Sentence denotes In EMSA, the (CCTTT)14 allele exhibited specific binding of nuclear proteins.
T12 1514-1526 Sentence denotes CONCLUSIONS:
T13 1527-1741 Sentence denotes These results, together with other studies on this gene and the CCTTT-microsatellite, establish, for the first time, a genetic association of iNOS with POAG and suggest a regulatory function for the microsatellite.