| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-89 |
Sentence |
denotes |
Allelic expression imbalance of human mu opioid receptor (OPRM1) caused by variant A118G. |
| T2 |
90-222 |
Sentence |
denotes |
As a primary target for opioid drugs and peptides, the mu opioid receptor (OPRM1) plays a key role in pain perception and addiction. |
| T3 |
223-456 |
Sentence |
denotes |
Genetic variants of OPRM1 have been implicated in predisposition to drug addiction, in particular the single nucleotide polymorphism A118G, leading to an N40D substitution, with an allele frequency of 10-32%, and uncertain functions. |
| T4 |
457-571 |
Sentence |
denotes |
We have measured allele-specific mRNA expression of OPRM1 in human autopsy brain tissues, using A118G as a marker. |
| T5 |
572-681 |
Sentence |
denotes |
In 8 heterozygous samples measured, the A118 mRNA allele was 1.5-2.5-fold more abundant than the G118 allele. |
| T6 |
682-1022 |
Sentence |
denotes |
Transfection into Chinese hamster ovary cells of a cDNA representing only the coding region of OPRM1, carrying adenosine, guanosine, cytidine, and thymidine in position 118, resulted in 1.5-fold lower mRNA levels only for OPRM1-G118, and more than 10-fold lower OPRM1 protein levels, measured by Western blotting and receptor binding assay. |
| T7 |
1023-1254 |
Sentence |
denotes |
After transfection and inhibition of transcription with actinomycin D, analysis of mRNA turnover failed to reveal differences in mRNA stability between A118 and G118 alleles, indicating a defect in transcription or mRNA maturation. |
| T8 |
1255-1374 |
Sentence |
denotes |
These results indicate that OPRM1-G118 is a functional variant with deleterious effects on both mRNA and protein yield. |
| T9 |
1375-1559 |
Sentence |
denotes |
Clarifying the functional relevance of polymorphisms associated with susceptibility to a complex disorder such as drug addiction provides a foundation for clinical association studies. |
