LitCoin-sentences  

PubMed:15461822 JSONTXT 27 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1 0-106 Sentence denotes Vaccine candidates derived from a novel infectious cDNA clone of an American genotype dengue virus type 2.
T2 107-118 Sentence denotes BACKGROUND:
T3 119-276 Sentence denotes A dengue virus type 2 (DEN-2 Tonga/74) isolated from a 1974 epidemic was characterized by mild illness and belongs to the American genotype of DEN-2 viruses.
T4 277-447 Sentence denotes To prepare a vaccine candidate, a previously described 30 nucleotide deletion (Delta30) in the 3' untranslated region of DEN-4 has been engineered into the DEN-2 isolate.
T5 448-456 Sentence denotes METHODS:
T6 457-581 Sentence denotes A full-length cDNA clone was generated from the DEN-2 virus and used to produce recombinant DEN-2 (rDEN-2) and rDEN2Delta30.
T7 582-729 Sentence denotes Viruses were evaluated for replication in SCID mice transplanted with human hepatoma cells (SCID-HuH-7 mice), in mosquitoes, and in rhesus monkeys.
T8 730-823 Sentence denotes Neutralizing antibody induction and protective efficacy were also assessed in rhesus monkeys.
T9 824-832 Sentence denotes RESULTS:
T10 833-945 Sentence denotes The rDEN2Delta30 virus was ten-fold reduced in replication in SCID-HuH-7 mice when compared to the parent virus.
T11 946-1058 Sentence denotes The rDEN-2 viruses were not infectious for Aedes mosquitoes, but both readily infected Toxorynchites mosquitoes.
T12 1059-1240 Sentence denotes In rhesus monkeys, rDEN2Delta30 appeared to be slightly attenuated when compared to the parent virus as measured by duration and peak of viremia and neutralizing antibody induction.
T13 1241-1472 Sentence denotes A derivative of rDEN2Delta30, designated rDEN2Delta30-4995, was generated by incorporation of a point mutation previously identified in the NS3 gene of DEN-4 and was found to be more attenuated than rDEN2Delta30 in SCID-HuH-7 mice.
T14 1473-1485 Sentence denotes CONCLUSIONS:
T15 1486-1626 Sentence denotes The rDEN2Delta30 and rDEN2Delta30-4995 viruses can be considered for evaluation in humans and for inclusion in a tetravalent dengue vaccine.