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PubMed:28479159 JSONTXT 30 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T1 0-107 Sentence denotes Proteomic analysis of filaggrin deficiency identifies molecular signatures characteristic of atopic eczema.
T1 0-107 Sentence denotes Proteomic analysis of filaggrin deficiency identifies molecular signatures characteristic of atopic eczema.
T2 108-119 Sentence denotes BACKGROUND:
T2 108-119 Sentence denotes BACKGROUND:
T3 120-195 Sentence denotes Atopic eczema (AE) is characterized by skin barrier and immune dysfunction.
T3 120-195 Sentence denotes Atopic eczema (AE) is characterized by skin barrier and immune dysfunction.
T4 196-386 Sentence denotes Null mutations in filaggrin (FLG), a key epidermal barrier protein, strongly predispose to AE; however, the precise role of FLG deficiency in AE pathogenesis remains incompletely understood.
T4 196-386 Sentence denotes Null mutations in filaggrin (FLG), a key epidermal barrier protein, strongly predispose to AE; however, the precise role of FLG deficiency in AE pathogenesis remains incompletely understood.
T5 387-398 Sentence denotes OBJECTIVES:
T5 387-398 Sentence denotes OBJECTIVES:
T6 399-580 Sentence denotes We sought to identify global proteomic changes downstream of FLG deficiency in human epidermal living skin-equivalent (LSE) models and validate findings in skin of patients with AE.
T6 399-580 Sentence denotes We sought to identify global proteomic changes downstream of FLG deficiency in human epidermal living skin-equivalent (LSE) models and validate findings in skin of patients with AE.
T7 581-589 Sentence denotes METHODS:
T7 581-589 Sentence denotes METHODS:
T8 590-877 Sentence denotes Differentially expressed proteins from paired control (nontargeting control short hairpin RNA [shNT]) and FLG knockdown (FLG knockdown short hairpin RNA [shFLG]) LSEs were identified by means of proteomic analysis (liquid chromatography-mass spectrometry) and Ingenuity Pathway Analysis.
T8 590-877 Sentence denotes Differentially expressed proteins from paired control (nontargeting control short hairpin RNA [shNT]) and FLG knockdown (FLG knockdown short hairpin RNA [shFLG]) LSEs were identified by means of proteomic analysis (liquid chromatography-mass spectrometry) and Ingenuity Pathway Analysis.
T9 878-1052 Sentence denotes Expression of key targets was validated in independent LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biopsy specimens (immunofluorescence).
T9 878-1052 Sentence denotes Expression of key targets was validated in independent LSE samples (quantitative RT-PCR and Western blotting) and in normal and AE skin biopsy specimens (immunofluorescence).
T10 1053-1061 Sentence denotes RESULTS:
T10 1053-1061 Sentence denotes RESULTS:
T11 1062-1264 Sentence denotes Proteomic analysis identified 17 (P ≤ .05) differentially expressed proteins after FLG knockdown, including kallikrein-7 (KLK7; 2.2-fold), cyclophilin A (PPIA; 0.9-fold), and cofilin-1 (CFL1, 1.3-fold).
T11 1062-1264 Sentence denotes Proteomic analysis identified 17 (P ≤ .05) differentially expressed proteins after FLG knockdown, including kallikrein-7 (KLK7; 2.2-fold), cyclophilin A (PPIA; 0.9-fold), and cofilin-1 (CFL1, 1.3-fold).
T12 1265-1385 Sentence denotes Differential protein expression was confirmed in shNT/shFLG LSEs; however, only KLK7 was transcriptionally dysregulated.
T12 1265-1385 Sentence denotes Differential protein expression was confirmed in shNT/shFLG LSEs; however, only KLK7 was transcriptionally dysregulated.
T13 1386-1510 Sentence denotes Molecular pathways overrepresented after FLG knockdown included inflammation, protease activity, cell structure, and stress.
T13 1386-1510 Sentence denotes Molecular pathways overrepresented after FLG knockdown included inflammation, protease activity, cell structure, and stress.
T14 1511-1675 Sentence denotes Furthermore, KLK7 (1.8-fold) and PPIA (0.65-fold) proteins were differentially expressed in lesional biopsy specimens from patients with AE relative to normal skin.
T14 1511-1675 Sentence denotes Furthermore, KLK7 (1.8-fold) and PPIA (0.65-fold) proteins were differentially expressed in lesional biopsy specimens from patients with AE relative to normal skin.
T15 1676-1688 Sentence denotes CONCLUSIONS:
T15 1676-1688 Sentence denotes CONCLUSIONS:
T16 1689-1856 Sentence denotes For the first time, we show that loss of FLG in the absence of inflammation is sufficient to alter the expression level of proteins relevant to the pathogenesis of AE.
T16 1689-1856 Sentence denotes For the first time, we show that loss of FLG in the absence of inflammation is sufficient to alter the expression level of proteins relevant to the pathogenesis of AE.
T17 1857-1945 Sentence denotes These include proteins regulating inflammatory, proteolytic, and cytoskeletal functions.
T17 1857-1945 Sentence denotes These include proteins regulating inflammatory, proteolytic, and cytoskeletal functions.
T18 1946-2157 Sentence denotes We identify PPIA as a novel protein with levels that are decreased in clinically active AE skin and show that the characteristic upregulation of KLK7 expression in patients with AE occurs downstream of FLG loss.
T18 1946-2157 Sentence denotes We identify PPIA as a novel protein with levels that are decreased in clinically active AE skin and show that the characteristic upregulation of KLK7 expression in patients with AE occurs downstream of FLG loss.
T19 2158-2297 Sentence denotes Importantly, we highlight disconnect between the epidermal proteome and transcriptome, emphasizing the utility of global proteomic studies.
T19 2158-2297 Sentence denotes Importantly, we highlight disconnect between the epidermal proteome and transcriptome, emphasizing the utility of global proteomic studies.