Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
353-518 |
Epistemic_statement |
denotes |
The objectives of the study were to isolate Escherichia coli from diarrheic calves, and to determine E. coli biotypes and risk factors associated with its isolation. |
T2 |
677-805 |
Epistemic_statement |
denotes |
Yellowish diarrhea, younger age, and lowcolostrum feeding were significantly associated with rate of E. coli isolation (P<0.05). |
T3 |
957-1191 |
Epistemic_statement |
denotes |
Finally, by comparing with studies elsewhere, from the 12 isolated biotypes, 3 of them were suggested to be enteropathogenic E. coli (EPEC), entherotoxigenic E. coli (ETEC), and adhesion and effacing E. coli (AEEC) pathogenic strains. |
T4 |
1404-1603 |
Epistemic_statement |
denotes |
Diarrhea is one of the very common disease syndromes in neonatal calves in different countries, and this can have severe impacts both economically and in terms of animal welfare (Africa Union 2008) . |
T5 |
1938-2239 |
Epistemic_statement |
denotes |
There is a multitude of interactions of the noninfectious causes (predisposing factors) (flaws or gaps in management-inadequate nutrition, exposure to severe environment, insufficient attention to the newborn calf, or a combination of these) and infectious causes (Blanchard 2012; Cho and Yoon 2014) . |
T6 |
3125-3293 |
Epistemic_statement |
denotes |
Many strains of the bacterium are harmless to the calf, but certain strains that acquire virulence genes can cause moderate to severe scours and even death (Wani et al. |
T7 |
4565-4718 |
Epistemic_statement |
denotes |
These tests are also easy to perform, by determining, whether a strain of E. coli will produce acid following growth in the presence of the carbohydrate. |
T8 |
4719-4882 |
Epistemic_statement |
denotes |
Of the various typing systems available, study of the organisms' biological properties (Bbiotyping^) appears to be a useful method of identification (Aklilu et al. |
T9 |
5469-5617 |
Epistemic_statement |
denotes |
However, there are frequent cases of calf diarrhea in the study area leading to morbidity and mortality and often seeking professional intervention. |
T10 |
5618-5825 |
Epistemic_statement |
denotes |
Therefore, this work was conducted to determine the diversity of E. coli using standard sugar fermentation tests and identify risk factors associated with its isolation from diarrheic calf feces in the area. |
T11 |
5826-5967 |
Epistemic_statement |
denotes |
We believe the data in this work contributes to diagnosis and control of calf diarrhea in Ethiopia, in particular, and worldwide, in general. |
T12 |
9681-9912 |
Epistemic_statement |
denotes |
The identified isolates were then further characterized for their sugar fermentation reactions on nine sugars, viz., dulcitol, raffinose, rhamnose, salicin, sucrose, inositol, lactose, maltose, and xylose according to Aklilu et al. |
T13 |
10401-10635 |
Epistemic_statement |
denotes |
Comparison was made between our biotype data and those published works; whenever a pathotype strain fermenting similar sugars with any of our biotypes was encountered, this strain was suggested as a possible pathotype for our biotype. |
T14 |
10636-10874 |
Epistemic_statement |
denotes |
Data describing the diarrhogenic conditions suggestive of E. coli infection observed on calves along with the amount of colostrum given, sex, weight, diarrhea type, and age were classified, filtered, and coded using Microsoft Excel® 2007. |
T15 |
11114-11244 |
Epistemic_statement |
denotes |
Chisquare (χ 2 ) was used to measure associations between the detection rates of E. coli and their biotypes with relevant factors. |
T16 |
11245-11329 |
Epistemic_statement |
denotes |
Associations were reported as statistically significant if P value is less than 5 %. |
T17 |
11707-11917 |
Epistemic_statement |
denotes |
There was a statistically significant association between rates of E. coli isolation and color type of diarrhea (P=0.001), isolation rates being highest in blood-tinged diarrhea and lowest in greenish diarrhea. |
T18 |
12415-12625 |
Epistemic_statement |
denotes |
There was no direct association between age of calves and occurrence of diarrhea; however, the isolation rate of E. coli was highest at the earlier age groups, decreasing as the age of diarrheic calf increases. |
T19 |
12850-13005 |
Epistemic_statement |
denotes |
It was also shown that isolation rate of E. coli was not statistically associated with either sex (P=0.703) or body weight (P =0.45) of the calves studied. |
T20 |
14063-14310 |
Epistemic_statement |
denotes |
Accordingly, by comparison with their data, the biotypes III, IVand VII, and VIII of the current study could possibly be entherotoxigenic E. coli (ETEC), enteropathogenic E. coli (EPEC), and adhesion and effacing E. coli (AEEC) strains (Table 4) . |
T21 |
14561-14694 |
Epistemic_statement |
denotes |
There was a statistically significant association between the different age groups and occurrence of the different biotypes (P<0.05). |
T22 |
15039-15311 |
Epistemic_statement |
denotes |
Furthermore, associations on the distribution of the different biotypes among diarrhea types were assessed; the predominant biotypes in the diarrhea types of yellowish, bloodtinged, pure bloody, and greenish were III; III, IV, and V; IV, and VIII, respectively (Table 6 ). |
T23 |
15312-15541 |
Epistemic_statement |
denotes |
Neonatal diarrhea is a major health problem in dairy farms, leading to high mortality and is hindering sustainable development of the dairy sector; E. coli is the most important cause of bacterial scours in calves (Charles et al. |
T24 |
15593-15730 |
Epistemic_statement |
denotes |
The involvement of E. coli in calf diarrhea has not been well studied in Ethiopia, with the exception of a recent study by Dawit (2012) . |
T25 |
15938-16037 |
Epistemic_statement |
denotes |
This finding is also consistent with the work of Amoki (2001) in the central high land of Ethiopia. |
T26 |
16038-16323 |
Epistemic_statement |
denotes |
The higher calf diarrhea due to E. coli in the present study might be attributed to the variations in age groups examined as well as environmental and management conditions of the farms such as insufficient and/or poor-quality colostrum intake by the calves as stated by Charles et al. |
T27 |
16444-16533 |
Epistemic_statement |
denotes |
Besides, a large dose of pathogenic E. coli may overcome colostral immunity (Quinn et al. |
T28 |
16786-16929 |
Epistemic_statement |
denotes |
The reason why the result of the current study varies from the reports in other areas might be due to variations in farm management conditions. |
T29 |
16990-17317 |
Epistemic_statement |
denotes |
(2007) , gaps in management specifically calf handling practices including inadequate nutrition, exposure to severe environment, insufficient attention to the newborn calf, or a combination of these, qualitative and ,133,135,137,138,139,167,168, 171,176,190,195 12 ( (100) a The sugar inositol has not been used by Johan et al. |
T30 |
17991-18145 |
Epistemic_statement |
denotes |
(2013) also reviewed that in calf scours due to E. coli, the small intestine may be filled with fluid and the large intestine may contain yellowish feces. |
T31 |
18146-18385 |
Epistemic_statement |
denotes |
Factors that may contribute to these differences between studies could be related to presence or absence of mixed infections, variations on management conditions of the farms, and the age of the calf as also supported by the present study. |
T32 |
18421-18594 |
Epistemic_statement |
denotes |
(2003) stated that onset of E. coli diarrhea varies in color and consistency that could be consistent with intestinal overload possibly due to high-volume colostrum feeding. |
T33 |
18759-18966 |
Epistemic_statement |
denotes |
Thus, the isolation rate of E. coli from blood-tinged diarrhea in the present study (9.0 %) was inconsistent with that of James and James (2003) , who isolated E. coli from 22 % of bloodtinged calf diarrhea. |
T34 |
19312-19453 |
Epistemic_statement |
denotes |
The overall variations in color of feces may be as a result of differences on the basis of pathogenic features and mechanisms of the disease. |
T35 |
19612-19767 |
Epistemic_statement |
denotes |
Diarrhea of the large intestine is associated with frequent small-volume stools with the presence of blood (Naylor and Smith 2002; Foster and Smith 2009) . |
T36 |
19768-19857 |
Epistemic_statement |
denotes |
Involvement of mixed infections could also complicate the appearance of feces (Ata et al. |
T37 |
20177-20298 |
Epistemic_statement |
denotes |
Furthermore, the association between the different age groups with the occurrence of diarrhea due to E. coli was studied. |
T38 |
20299-20484 |
Epistemic_statement |
denotes |
The highest percentage (22.9 %) of E. coli-positive (23) samples were identified in those calves found in the 1-7 days age group; this result is inconsistent with reports of Achá et al. |
T39 |
20500-20614 |
Epistemic_statement |
denotes |
The causes of variation might be as a result of variations in management of the farms and immunity of the animals. |
T40 |
20745-20899 |
Epistemic_statement |
denotes |
The author stated that young neonates under 1 week of age are particularly susceptible because the normal flora of the intestine is not fully established. |
T41 |
21717-21894 |
Epistemic_statement |
denotes |
This could be due to the poorly developed immune system of the days old calves as compared to older ones to fight against the disease-causing agents (Darsema 2008; Bekele et al. |
T42 |
22112-22223 |
Epistemic_statement |
denotes |
The cause of high isolation rate in the calves receiving less amount of colostrum could be for various reasons. |
T43 |
22548-22639 |
Epistemic_statement |
denotes |
In addition, FPT could be due to bacterial contamination of the fed colostrum (Keith et al. |
T44 |
22648-22819 |
Epistemic_statement |
denotes |
Colostrum feeding practices also have effect in that allowing calves to nurse their dam may predispose them to FPT since they consume late and small amount (Meganck et al. |
T45 |
23543-23645 |
Epistemic_statement |
denotes |
So according to their data, biotype III of the present study could be ETEC (entherotoxigenic E. coli). |
T46 |
24458-24591 |
Epistemic_statement |
denotes |
One of the limitations of this study was the lack of pathotype strain identification of the E. coli isolates due to lack of resource. |
T47 |
25055-25144 |
Epistemic_statement |
denotes |
Serotyping showed a close relationship between biotype and serotype of the AEEC examined. |
T48 |
25582-25665 |
Epistemic_statement |
denotes |
In reference to their work, biotypes IV and VII of the present study could be AEEC. |
T49 |
25752-25836 |
Epistemic_statement |
denotes |
(1988) might be the result of differences in sample size and animal species studied. |
T50 |
26681-26749 |
Epistemic_statement |
denotes |
So, biotype VIII of the present study could be suggested to be EPEC. |
T51 |
26750-26850 |
Epistemic_statement |
denotes |
In the present study, associations between age groups and the different biotypes were also assessed. |
T52 |
27268-27492 |
Epistemic_statement |
denotes |
In conclusion, during the present study, calf diarrhea was investigated at Kombolcha town in order to isolate E. coli and determine the biotypes of the isolates as well as identify risk factors associated with calf diarrhea. |
T53 |
27810-27893 |
Epistemic_statement |
denotes |
Biotyping is relatively cheaper and can be conducted by less-experienced personnel. |
T54 |
27894-28003 |
Epistemic_statement |
denotes |
Compared to molecular and serotyping techniques, the technique would be valuable for resource poor countries. |
T55 |
28004-28216 |
Epistemic_statement |
denotes |
However, further studies involving comparison of biotyping with PCR and serotyping should be conducted in order to study the applicability of the method for strain identification among pathogenic E. coli strains. |