CORD-19-Sentences  

CORD-19:4c6a45d1e56660fa713b637825b96e287a0acef5 JSONTXT 8 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-73 Sentence denotes Experimental infection of dogs with a feline endogenous retrovirus RD-114
TextSentencer_T1 0-73 Sentence denotes Experimental infection of dogs with a feline endogenous retrovirus RD-114
TextSentencer_T2 75-83 Sentence denotes Abstract
TextSentencer_T2 75-83 Sentence denotes Abstract
TextSentencer_T3 84-95 Sentence denotes Background:
TextSentencer_T3 84-95 Sentence denotes Background:
TextSentencer_T4 96-170 Sentence denotes The feline endogenous retrovirus RD114 is contained in the genome of cats.
TextSentencer_T4 96-170 Sentence denotes The feline endogenous retrovirus RD114 is contained in the genome of cats.
TextSentencer_T5 171-249 Sentence denotes The virus may contaminate live canine vaccines based on cultured feline cells.
TextSentencer_T5 171-249 Sentence denotes The virus may contaminate live canine vaccines based on cultured feline cells.
TextSentencer_T6 250-401 Sentence denotes The in vivo infectivity, acute and subacute pathogenicity, and viral proliferation of the RD114 virus were evaluated by experimental infection of dogs.
TextSentencer_T6 250-401 Sentence denotes The in vivo infectivity, acute and subacute pathogenicity, and viral proliferation of the RD114 virus were evaluated by experimental infection of dogs.
TextSentencer_T7 402-410 Sentence denotes Methods:
TextSentencer_T7 402-410 Sentence denotes Methods:
TextSentencer_T8 411-535 Sentence denotes Nine specific pathogen free dogs were divided into three groups, with each group consisting of one female and two male dogs.
TextSentencer_T8 411-535 Sentence denotes Nine specific pathogen free dogs were divided into three groups, with each group consisting of one female and two male dogs.
TextSentencer_T9 536-731 Sentence denotes Dogs were subcutaneously inoculated in the neck with either 1 ml RD114 stock virus (group A), inactivated RD114 virus suspension (group B), or cell culture medium (group C) as a negative control.
TextSentencer_T9 536-731 Sentence denotes Dogs were subcutaneously inoculated in the neck with either 1 ml RD114 stock virus (group A), inactivated RD114 virus suspension (group B), or cell culture medium (group C) as a negative control.
TextSentencer_T10 732-928 Sentence denotes To assess blood cell counts and biochemical properties, blood samples from each group were collected 5 days before inoculation, just prior to inoculation, and 1, 3, 7 and 10 days post-inoculation.
TextSentencer_T10 732-928 Sentence denotes To assess blood cell counts and biochemical properties, blood samples from each group were collected 5 days before inoculation, just prior to inoculation, and 1, 3, 7 and 10 days post-inoculation.
TextSentencer_T11 929-936 Sentence denotes Result:
TextSentencer_T11 929-936 Sentence denotes Result:
TextSentencer_T12 937-1212 Sentence denotes During the experimental period of 51 days, none of the dogs inoculated with RD114 virus showed any clinical signs, significant increases in rectal temperature or abnormal blood biochemical characteristics including C-reactive protein when compared with the negative controls.
TextSentencer_T12 937-1212 Sentence denotes During the experimental period of 51 days, none of the dogs inoculated with RD114 virus showed any clinical signs, significant increases in rectal temperature or abnormal blood biochemical characteristics including C-reactive protein when compared with the negative controls.
TextSentencer_T13 1213-1298 Sentence denotes We were not able to re-isolate the RD114 virus from buffy coat cells of group A dogs.
TextSentencer_T13 1213-1298 Sentence denotes We were not able to re-isolate the RD114 virus from buffy coat cells of group A dogs.
TextSentencer_T14 1299-1459 Sentence denotes Additionally, we could not detect RD114 provirus in the genomic DNA isolated from peripheral blood leukocytes, lymph node, spleen and sternal bone marrow cells.
TextSentencer_T14 1299-1459 Sentence denotes Additionally, we could not detect RD114 provirus in the genomic DNA isolated from peripheral blood leukocytes, lymph node, spleen and sternal bone marrow cells.
TextSentencer_T15 1460-1472 Sentence denotes Conclusions:
TextSentencer_T15 1460-1472 Sentence denotes Conclusions:
TextSentencer_T16 1473-1560 Sentence denotes Signs of RD114 virus proliferation were not found after subcutaneous infection of dogs.
TextSentencer_T16 1473-1560 Sentence denotes Signs of RD114 virus proliferation were not found after subcutaneous infection of dogs.
TextSentencer_T17 1561-1734 Sentence denotes Although the potential risk caused by infection with RD114 virus in dogs could not be assessed in this study, we suspect that RD114 virus has little or no virulence in dogs.
TextSentencer_T17 1561-1734 Sentence denotes Although the potential risk caused by infection with RD114 virus in dogs could not be assessed in this study, we suspect that RD114 virus has little or no virulence in dogs.
TextSentencer_T18 1736-1850 Sentence denotes Domestic cats are generally assumed to harbour the infectious endogenous retrovirus RD114 in their genome [1, 2] .
TextSentencer_T18 1736-1850 Sentence denotes Domestic cats are generally assumed to harbour the infectious endogenous retrovirus RD114 in their genome [1, 2] .
TextSentencer_T19 1851-1956 Sentence denotes It is known that the Crandell-Rees feline kidney cell line is contaminated with an RD114-like virus [3] .
TextSentencer_T19 1851-1956 Sentence denotes It is known that the Crandell-Rees feline kidney cell line is contaminated with an RD114-like virus [3] .
TextSentencer_T20 1957-2086 Sentence denotes Recently, Miyazawa et al. [4] found that certain live attenuated vaccines for dogs were contaminated with infectious RD114 virus.
TextSentencer_T20 1957-2086 Sentence denotes Recently, Miyazawa et al. [4] found that certain live attenuated vaccines for dogs were contaminated with infectious RD114 virus.
TextSentencer_T21 2087-2267 Sentence denotes We also confirmed in our laboratory that infectious RD114 virus was present in certain live attenuated canine vaccines that were manufactured using feline cells (unpublished data).
TextSentencer_T21 2087-2267 Sentence denotes We also confirmed in our laboratory that infectious RD114 virus was present in certain live attenuated canine vaccines that were manufactured using feline cells (unpublished data).
TextSentencer_T22 2268-2460 Sentence denotes The amount of infectious RD114 virus found in manufactured live canine vaccines was as high as 1,800 50% tissue culture infective dose (TCID 50 )/vial (one vial represents a single dose) [4] .
TextSentencer_T22 2268-2460 Sentence denotes The amount of infectious RD114 virus found in manufactured live canine vaccines was as high as 1,800 50% tissue culture infective dose (TCID 50 )/vial (one vial represents a single dose) [4] .
TextSentencer_T23 2461-2657 Sentence denotes RD114 virus can be regarded as an 'exogenous' retrovirus in non-feline species including dogs, however there is no information concerning the etiological features of RD114 virus infection in dogs.
TextSentencer_T23 2461-2657 Sentence denotes RD114 virus can be regarded as an 'exogenous' retrovirus in non-feline species including dogs, however there is no information concerning the etiological features of RD114 virus infection in dogs.
TextSentencer_T24 2658-2867 Sentence denotes The present study was conducted to evaluate the in vivo infectivity, acute and subacute pathogenicity, and viral proliferation of the RD114 virus by experimental infection of specific pathogen free (SPF) dogs.
TextSentencer_T24 2658-2867 Sentence denotes The present study was conducted to evaluate the in vivo infectivity, acute and subacute pathogenicity, and viral proliferation of the RD114 virus by experimental infection of specific pathogen free (SPF) dogs.
TextSentencer_T25 2868-2957 Sentence denotes A LacZ marker rescue assay was used to detect and titrate infectious RD114 virus [5, 6] .
TextSentencer_T25 2868-2957 Sentence denotes A LacZ marker rescue assay was used to detect and titrate infectious RD114 virus [5, 6] .
TextSentencer_T26 2958-3137 Sentence denotes The principle of the assay is based on the detection of infectious RD114 virus using TE671 (human rhabdomyosarcoma) cells transduced with the LacZ marker gene [TE671(LacZ) cells].
TextSentencer_T26 2958-3137 Sentence denotes The principle of the assay is based on the detection of infectious RD114 virus using TE671 (human rhabdomyosarcoma) cells transduced with the LacZ marker gene [TE671(LacZ) cells].
TextSentencer_T27 3138-3248 Sentence denotes RD114 virus was prepared from the culture supernatant of TE671 cells chronically infected with the virus [7] .
TextSentencer_T27 3138-3248 Sentence denotes RD114 virus was prepared from the culture supernatant of TE671 cells chronically infected with the virus [7] .
TextSentencer_T28 3249-3374 Sentence denotes Culture supernatants were filtered through a 0.45 μm pore size membrane filters, and aliquots stored at -80°C until required.
TextSentencer_T28 3249-3374 Sentence denotes Culture supernatants were filtered through a 0.45 μm pore size membrane filters, and aliquots stored at -80°C until required.
TextSentencer_T29 3375-3447 Sentence denotes The titre of the stock virus was approximately 10 5 infectious units/ml.
TextSentencer_T29 3375-3447 Sentence denotes The titre of the stock virus was approximately 10 5 infectious units/ml.
TextSentencer_T30 3448-3642 Sentence denotes To prepare inactivated RD114 virus as inocula, the stock virus was added to an equal volume of diethylether, then vigorously and intermittently mixed at room temperature (around 20°C) for 3 min.
TextSentencer_T30 3448-3642 Sentence denotes To prepare inactivated RD114 virus as inocula, the stock virus was added to an equal volume of diethylether, then vigorously and intermittently mixed at room temperature (around 20°C) for 3 min.
TextSentencer_T31 3643-3760 Sentence denotes Absence of infectious RD114 virus in the inactivated viral preparation was confirmed by the LacZ marker rescue assay.
TextSentencer_T31 3643-3760 Sentence denotes Absence of infectious RD114 virus in the inactivated viral preparation was confirmed by the LacZ marker rescue assay.
TextSentencer_T32 3761-3873 Sentence denotes Nine 10-months-old SPF beagles (six males and three females) were divided into three groups (groups A, B and C).
TextSentencer_T32 3761-3873 Sentence denotes Nine 10-months-old SPF beagles (six males and three females) were divided into three groups (groups A, B and C).
TextSentencer_T33 3874-3966 Sentence denotes Each group consisted of one female and two male dogs with individual dogs confined to cages.
TextSentencer_T33 3874-3966 Sentence denotes Each group consisted of one female and two male dogs with individual dogs confined to cages.
TextSentencer_T34 3967-4168 Sentence denotes The dogs were inoculated subcutaneously in the neck with either 1 ml of RD114 stock virus (group A), inactivated RD114 virus suspension (group B) or cell culture medium (group C) as a negative control.
TextSentencer_T34 3967-4168 Sentence denotes The dogs were inoculated subcutaneously in the neck with either 1 ml of RD114 stock virus (group A), inactivated RD114 virus suspension (group B) or cell culture medium (group C) as a negative control.
TextSentencer_T35 4169-4276 Sentence denotes The inoculation route was chosen according to the manufacturer's instructions for the live canine vaccines.
TextSentencer_T35 4169-4276 Sentence denotes The inoculation route was chosen according to the manufacturer's instructions for the live canine vaccines.
TextSentencer_T36 4277-4458 Sentence denotes All animal studies were conducted in accordance with the National Veterinary Assay Laboratory Guide for the Care and Use of Laboratory Animals, and the relevant Animal Welfare Acts.
TextSentencer_T36 4277-4458 Sentence denotes All animal studies were conducted in accordance with the National Veterinary Assay Laboratory Guide for the Care and Use of Laboratory Animals, and the relevant Animal Welfare Acts.
TextSentencer_T37 4459-4528 Sentence denotes The dogs were euthanized 51 days post inoculation (PI) and autopsied.
TextSentencer_T37 4459-4528 Sentence denotes The dogs were euthanized 51 days post inoculation (PI) and autopsied.
TextSentencer_T38 4529-4613 Sentence denotes The thoracic organs, abdominal organs and bone marrow were macroscopically examined.
TextSentencer_T38 4529-4613 Sentence denotes The thoracic organs, abdominal organs and bone marrow were macroscopically examined.
TextSentencer_T39 4614-4723 Sentence denotes The axillary lymph nodes, spleen and sternal bone marrow were collected for further virological examinations.
TextSentencer_T39 4614-4723 Sentence denotes The axillary lymph nodes, spleen and sternal bone marrow were collected for further virological examinations.
TextSentencer_T40 4724-4825 Sentence denotes Re-isolation of the RD114 virus was attempted by coculturing TE671(LacZ) cells with buffy coat cells.
TextSentencer_T40 4724-4825 Sentence denotes Re-isolation of the RD114 virus was attempted by coculturing TE671(LacZ) cells with buffy coat cells.
TextSentencer_T41 4826-5023 Sentence denotes To assess the blood cell counts and biochemical properties, blood samples from each group were collected 5 days before inoculation, just prior to inoculation (day 0), and at 1, 3, 7 and 10 days PI.
TextSentencer_T41 4826-5023 Sentence denotes To assess the blood cell counts and biochemical properties, blood samples from each group were collected 5 days before inoculation, just prior to inoculation (day 0), and at 1, 3, 7 and 10 days PI.
TextSentencer_T42 5024-5246 Sentence denotes White blood cells (WBC), red blood cells, haemoglobin, haematocrit, mean cell volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, and platelet numbers were determined in the blood cell counts.
TextSentencer_T42 5024-5246 Sentence denotes White blood cells (WBC), red blood cells, haemoglobin, haematocrit, mean cell volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, and platelet numbers were determined in the blood cell counts.
TextSentencer_T43 5247-5611 Sentence denotes The biochemical analyses included total protein, albumin, total bilirubin, glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase, alkaline phosphatase, lactic dehydrogenase, amylase, lipase, blood urea nitrogen, creatinine, total cholesterol, triglyceride, sodium, potassium, chlorine, calcium, inorganic phosphorous, glucose and total bile acid levels.
TextSentencer_T43 5247-5611 Sentence denotes The biochemical analyses included total protein, albumin, total bilirubin, glutamic-oxaloacetic transaminase, glutamic-pyruvic transaminase, alkaline phosphatase, lactic dehydrogenase, amylase, lipase, blood urea nitrogen, creatinine, total cholesterol, triglyceride, sodium, potassium, chlorine, calcium, inorganic phosphorous, glucose and total bile acid levels.
TextSentencer_T44 5612-5740 Sentence denotes The C-reactive protein (CRP) concentration, a sensitive indicator of inflammation in dogs, was analysed at days 0, 7, and 51 PI.
TextSentencer_T44 5612-5740 Sentence denotes The C-reactive protein (CRP) concentration, a sensitive indicator of inflammation in dogs, was analysed at days 0, 7, and 51 PI.
TextSentencer_T45 5741-5897 Sentence denotes Additionally, during the experimental period, clinical signs were recorded daily, and rectal temperature and body weight were measured at least once a week.
TextSentencer_T45 5741-5897 Sentence denotes Additionally, during the experimental period, clinical signs were recorded daily, and rectal temperature and body weight were measured at least once a week.
TextSentencer_T46 5898-6096 Sentence denotes Genomic DNA was extracted from ethylenediaminetetraacetic acid (EDTA) stabilized whole blood, axillary lymph nodes, spleen and bone marrow, and subjected to polymerase chain reaction (PCR) analysis.
TextSentencer_T46 5898-6096 Sentence denotes Genomic DNA was extracted from ethylenediaminetetraacetic acid (EDTA) stabilized whole blood, axillary lymph nodes, spleen and bone marrow, and subjected to polymerase chain reaction (PCR) analysis.
TextSentencer_T47 6097-6212 Sentence denotes In addition, genomic DNA was also extracted from TE671(LacZ) cells, 12 days after co-culture with buffy coat cells.
TextSentencer_T47 6097-6212 Sentence denotes In addition, genomic DNA was also extracted from TE671(LacZ) cells, 12 days after co-culture with buffy coat cells.
TextSentencer_T48 6213-6336 Sentence denotes The PCR was conducted as described by Sakaguchi et al. [6] by amplifying a portion of the env and pol genes of RD114 virus.
TextSentencer_T48 6213-6336 Sentence denotes The PCR was conducted as described by Sakaguchi et al. [6] by amplifying a portion of the env and pol genes of RD114 virus.
TextSentencer_T49 6337-6495 Sentence denotes The single step PCR assay was performed on genomic DNA extracted from TE671(LacZ) cells infected with ten-fold serially diluted (10 0 to 10 -10 ) stock virus.
TextSentencer_T49 6337-6495 Sentence denotes The single step PCR assay was performed on genomic DNA extracted from TE671(LacZ) cells infected with ten-fold serially diluted (10 0 to 10 -10 ) stock virus.
TextSentencer_T50 6496-6579 Sentence denotes The limit of detection was 10 -5 and 10 -6 for the env and pol genes, respectively.
TextSentencer_T50 6496-6579 Sentence denotes The limit of detection was 10 -5 and 10 -6 for the env and pol genes, respectively.
TextSentencer_T51 6580-6716 Sentence denotes Then, the LacZ pseudotype virus positive cells were detected 17, 2 and 0 cells for 10 -4 , 10 -5 and 10 -6 diluted points, respectively.
TextSentencer_T51 6580-6716 Sentence denotes Then, the LacZ pseudotype virus positive cells were detected 17, 2 and 0 cells for 10 -4 , 10 -5 and 10 -6 diluted points, respectively.
TextSentencer_T52 6717-6789 Sentence denotes The mean values of all measured variables of each group were calculated.
TextSentencer_T52 6717-6789 Sentence denotes The mean values of all measured variables of each group were calculated.
TextSentencer_T53 6790-6859 Sentence denotes Statistical analyses were performed using SPSS software (version 13).
TextSentencer_T53 6790-6859 Sentence denotes Statistical analyses were performed using SPSS software (version 13).
TextSentencer_T54 6860-6986 Sentence denotes The differences between the three groups at each time point were compared with P values of <0.05 considered to be significant.
TextSentencer_T54 6860-6986 Sentence denotes The differences between the three groups at each time point were compared with P values of <0.05 considered to be significant.
TextSentencer_T55 6987-7237 Sentence denotes During the experimental period, none of the dogs in groups A and B showed any clinical signs, including significant increases in rectal temperature, or distinct abnormal biochemical blood characteristics including CRP when compared with group C dogs.
TextSentencer_T55 6987-7237 Sentence denotes During the experimental period, none of the dogs in groups A and B showed any clinical signs, including significant increases in rectal temperature, or distinct abnormal biochemical blood characteristics including CRP when compared with group C dogs.
TextSentencer_T56 7238-7398 Sentence denotes In addition, the WBC counts of group A and B dogs were nearly equal to those of group C, and body weight increased in all animals after inoculation (Figure 1 ).
TextSentencer_T56 7238-7398 Sentence denotes In addition, the WBC counts of group A and B dogs were nearly equal to those of group C, and body weight increased in all animals after inoculation (Figure 1 ).
TextSentencer_T57 7399-7545 Sentence denotes Statistical analysis showed that the only significant difference was seen in the potassium level in the three groups five days before inoculation.
TextSentencer_T57 7399-7545 Sentence denotes Statistical analysis showed that the only significant difference was seen in the potassium level in the three groups five days before inoculation.
TextSentencer_T58 7546-7629 Sentence denotes At autopsy (51 days PI), any significant lesions were not observed macroscopically.
TextSentencer_T58 7546-7629 Sentence denotes At autopsy (51 days PI), any significant lesions were not observed macroscopically.
TextSentencer_T59 7630-7774 Sentence denotes Major lymph nodes, especially the axillary lymph nodes, were small, ranging from 0.5-1 cm in diameter with distinct cortico-medullary junctions.
TextSentencer_T59 7630-7774 Sentence denotes Major lymph nodes, especially the axillary lymph nodes, were small, ranging from 0.5-1 cm in diameter with distinct cortico-medullary junctions.
TextSentencer_T60 7775-7842 Sentence denotes The spleen and bone marrow did not contain any significant lesions.
TextSentencer_T60 7775-7842 Sentence denotes The spleen and bone marrow did not contain any significant lesions.
TextSentencer_T61 7843-7976 Sentence denotes Infectious RD114 virus was not detected from TE671 (LacZ) cells co-cultured with buffy coat cells using the LacZ marker rescue assay.
TextSentencer_T61 7843-7976 Sentence denotes Infectious RD114 virus was not detected from TE671 (LacZ) cells co-cultured with buffy coat cells using the LacZ marker rescue assay.
TextSentencer_T62 7977-8082 Sentence denotes This finding was also confirmed by PCR assays using genomic DNA from the co-cultured cells as a template.
TextSentencer_T62 7977-8082 Sentence denotes This finding was also confirmed by PCR assays using genomic DNA from the co-cultured cells as a template.
TextSentencer_T63 8083-8261 Sentence denotes Additionally, RD114 provirus was not detected in genomic DNA extracted from peripheral blood, lymph nodes, spleen and sternal bone marrow, using the one-step PCR assays ( Figure.
TextSentencer_T63 8083-8261 Sentence denotes Additionally, RD114 provirus was not detected in genomic DNA extracted from peripheral blood, lymph nodes, spleen and sternal bone marrow, using the one-step PCR assays ( Figure.
TextSentencer_T64 8262-8265 Sentence denotes 2).
TextSentencer_T64 8262-8265 Sentence denotes 2).
TextSentencer_T65 8266-8393 Sentence denotes It has been reported that RD114 virus infects a variety of canine cell lines, such as the Mardin-Darby canine kidney line [8] .
TextSentencer_T65 8266-8393 Sentence denotes It has been reported that RD114 virus infects a variety of canine cell lines, such as the Mardin-Darby canine kidney line [8] .
TextSentencer_T66 8394-8560 Sentence denotes RD114 virus also actively infects cells from cats and dogs, and may be transmitted to nonfeline species because of the xenotropic features of the virus in vitro [9] .
TextSentencer_T66 8394-8560 Sentence denotes RD114 virus also actively infects cells from cats and dogs, and may be transmitted to nonfeline species because of the xenotropic features of the virus in vitro [9] .
TextSentencer_T67 8561-8687 Sentence denotes Therefore, if dogs are exposed to a high dose of RD114 virus, the viral genome may integrate into the cells of target tissues.
TextSentencer_T67 8561-8687 Sentence denotes Therefore, if dogs are exposed to a high dose of RD114 virus, the viral genome may integrate into the cells of target tissues.
TextSentencer_T68 8688-8756 Sentence denotes Viraemia is often associated with the acute phase of viral diseases.
TextSentencer_T68 8688-8756 Sentence denotes Viraemia is often associated with the acute phase of viral diseases.
TextSentencer_T69 8757-8851 Sentence denotes In clinical cases caused by retroviruses, blood specimens are mainly used for virus detection.
TextSentencer_T69 8757-8851 Sentence denotes In clinical cases caused by retroviruses, blood specimens are mainly used for virus detection.
TextSentencer_T70 8852-9086 Sentence denotes However, we were unable to detect RD114 provirus from blood, lymph node, spleen and sternal bone marrow samples using a one-step PCR assay, despite group A dogs being inoculated with approximately 10 5 infectious units of RD114 virus.
TextSentencer_T70 8852-9086 Sentence denotes However, we were unable to detect RD114 provirus from blood, lymph node, spleen and sternal bone marrow samples using a one-step PCR assay, despite group A dogs being inoculated with approximately 10 5 infectious units of RD114 virus.
TextSentencer_T71 9087-9273 Sentence denotes These results suggest that RD114 did not proliferate and disseminate in the dogs, or that RD114 virus did not proliferate efficiently in the blood cells and hematopoietic system of dogs.
TextSentencer_T71 9087-9273 Sentence denotes These results suggest that RD114 did not proliferate and disseminate in the dogs, or that RD114 virus did not proliferate efficiently in the blood cells and hematopoietic system of dogs.
TextSentencer_T72 9274-9442 Sentence denotes The virus stock, neutralized with the sera of each dog, was inoculated onto TE671(LacZ) cells, and the number of lacZ positive foci among the three groups were counted.
TextSentencer_T72 9274-9442 Sentence denotes The virus stock, neutralized with the sera of each dog, was inoculated onto TE671(LacZ) cells, and the number of lacZ positive foci among the three groups were counted.
TextSentencer_T73 9443-9610 Sentence denotes In the present study, the antibody titres remained unchanged in all dogs following inoculation (data not shown), implying that RD114 virus did not proliferate in dogs.
TextSentencer_T73 9443-9610 Sentence denotes In the present study, the antibody titres remained unchanged in all dogs following inoculation (data not shown), implying that RD114 virus did not proliferate in dogs.
TextSentencer_T74 9611-9689 Sentence denotes The findings indicate that the RD114 virus has little or no virulence in dogs.
TextSentencer_T74 9611-9689 Sentence denotes The findings indicate that the RD114 virus has little or no virulence in dogs.
TextSentencer_T75 9690-9881 Sentence denotes The potential risk caused by infection with RD114 virus in dogs cannot be accurately assessed because a longer PI period is required for the verification or exclusion of retroviral infection.
TextSentencer_T75 9690-9881 Sentence denotes The potential risk caused by infection with RD114 virus in dogs cannot be accurately assessed because a longer PI period is required for the verification or exclusion of retroviral infection.
TextSentencer_T76 9882-9986 Sentence denotes It is also possible that dogs younger than 10-months might be more susceptible to RD114 virus infection.
TextSentencer_T76 9882-9986 Sentence denotes It is also possible that dogs younger than 10-months might be more susceptible to RD114 virus infection.
TextSentencer_T77 9987-10051 Sentence denotes Millions of puppies are vaccinated worldwide on an annual basis.
TextSentencer_T77 9987-10051 Sentence denotes Millions of puppies are vaccinated worldwide on an annual basis.
TextSentencer_T78 10052-10135 Sentence denotes Therefore it is impossible to completely rule out adverse effects of the infection.
TextSentencer_T78 10052-10135 Sentence denotes Therefore it is impossible to completely rule out adverse effects of the infection.
TextSentencer_T79 10136-10415 Sentence denotes Many exogenous retroviruses cause leukaemia and tumours in a wide variety of animal species, and further research is required to isolate or detect RD114 virus in dogs that have developed leukaemia and tumours following use of live canine vaccines manufactured using feline cells.
TextSentencer_T79 10136-10415 Sentence denotes Many exogenous retroviruses cause leukaemia and tumours in a wide variety of animal species, and further research is required to isolate or detect RD114 virus in dogs that have developed leukaemia and tumours following use of live canine vaccines manufactured using feline cells.
TextSentencer_T80 10416-10540 Sentence denotes Hopefully, such research would clarify the possible relationship between the occurrence of diseases in dogs and vaccination.
TextSentencer_T80 10416-10540 Sentence denotes Hopefully, such research would clarify the possible relationship between the occurrence of diseases in dogs and vaccination.
TextSentencer_T81 10541-10628 Sentence denotes Signs of RD114 virus proliferation were not found after subcutaneous infection of dogs.
TextSentencer_T81 10541-10628 Sentence denotes Signs of RD114 virus proliferation were not found after subcutaneous infection of dogs.
TextSentencer_T82 10629-10871 Sentence denotes Although the potential risk caused by infection with RD114 virus in dogs could not be assessed in this study because the minimum infectious dose and pathogenicity remain unknown, we suspect that RD114 virus has little or no virulence in dogs.
TextSentencer_T82 10629-10871 Sentence denotes Although the potential risk caused by infection with RD114 virus in dogs could not be assessed in this study because the minimum infectious dose and pathogenicity remain unknown, we suspect that RD114 virus has little or no virulence in dogs.
TextSentencer_T83 10872-10890 Sentence denotes Abbreviations SPF:
TextSentencer_T83 10872-10890 Sentence denotes Abbreviations SPF:
TextSentencer_T84 10891-10914 Sentence denotes Specific pathogen free.
TextSentencer_T84 10891-10914 Sentence denotes Specific pathogen free.
TextSentencer_T85 10915-11100 Sentence denotes The dogs were free from infections with canine adenovirus (CAdV-2), canine coronavirus, canine distempervirus, canine parainfluenzavirus, canine parvovirus, leptospira and rabies virus.
TextSentencer_T85 10915-11100 Sentence denotes The dogs were free from infections with canine adenovirus (CAdV-2), canine coronavirus, canine distempervirus, canine parainfluenzavirus, canine parvovirus, leptospira and rabies virus.