CORD-19-Sentences  

CORD-19:4c04431830f520a349d5535bb15326c2553207b9 JSONTXT 11 Projects

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-100 Sentence denotes Antigenic Variation of Porcine Transmissible Gastroenteritis Virus Detected by Monoclonal Antibodies
TextSentencer_T1 0-100 Sentence denotes Antigenic Variation of Porcine Transmissible Gastroenteritis Virus Detected by Monoclonal Antibodies
TextSentencer_T1 0-100 Sentence denotes Antigenic Variation of Porcine Transmissible Gastroenteritis Virus Detected by Monoclonal Antibodies
TextSentencer_T2 102-110 Sentence denotes Abstract
TextSentencer_T2 102-110 Sentence denotes Abstract
TextSentencer_T2 102-110 Sentence denotes Abstract
TextSentencer_T3 111-200 Sentence denotes Hohdatsu, T., Eiguchi, Y., Tsuchimoto, M., Ide, S., Yamagishi, H. and Matumoto, M., 1987.
TextSentencer_T3 111-200 Sentence denotes Hohdatsu, T., Eiguchi, Y., Tsuchimoto, M., Ide, S., Yamagishi, H. and Matumoto, M., 1987.
TextSentencer_T3 111-200 Sentence denotes Hohdatsu, T., Eiguchi, Y., Tsuchimoto, M., Ide, S., Yamagishi, H. and Matumoto, M., 1987.
TextSentencer_T4 201-302 Sentence denotes Antigenic variation of porcine transmissible gastroenteritis virus detected by monoclonal antibodies.
TextSentencer_T4 201-302 Sentence denotes Antigenic variation of porcine transmissible gastroenteritis virus detected by monoclonal antibodies.
TextSentencer_T4 201-302 Sentence denotes Antigenic variation of porcine transmissible gastroenteritis virus detected by monoclonal antibodies.
TextSentencer_T5 303-307 Sentence denotes Vet.
TextSentencer_T5 303-307 Sentence denotes Vet.
TextSentencer_T5 303-307 Sentence denotes Vet.
TextSentencer_T6 308-323 Sentence denotes Microbiol., 14:
TextSentencer_T6 308-323 Sentence denotes Microbiol., 14:
TextSentencer_T6 308-323 Sentence denotes Microbiol., 14:
TextSentencer_T7 324-332 Sentence denotes 115-124.
TextSentencer_T7 324-332 Sentence denotes 115-124.
TextSentencer_T7 324-332 Sentence denotes 115-124.
TextSentencer_T8 333-641 Sentence denotes Mouse myeloma cells (SP2/O) were fused with spleen cells from BALB/c mice immunized with detergent-solubilized antigen of purified virus, and 21 monoclonal (MC) antibodies reactive in enzyme-linked immunosorbent assay with the T0-163 strain of porcine transmissible gastroenteritis (TGE) virus were obtained.
TextSentencer_T8 333-641 Sentence denotes Mouse myeloma cells (SP2/O) were fused with spleen cells from BALB/c mice immunized with detergent-solubilized antigen of purified virus, and 21 monoclonal (MC) antibodies reactive in enzyme-linked immunosorbent assay with the T0-163 strain of porcine transmissible gastroenteritis (TGE) virus were obtained.
TextSentencer_T8 333-641 Sentence denotes Mouse myeloma cells (SP2/O) were fused with spleen cells from BALB/c mice immunized with detergent-solubilized antigen of purified virus, and 21 monoclonal (MC) antibodies reactive in enzyme-linked immunosorbent assay with the T0-163 strain of porcine transmissible gastroenteritis (TGE) virus were obtained.
TextSentencer_T9 642-717 Sentence denotes Of these MC antibodies, 14, 6 and 1 were IgG1, IgG2a and IgM, respectively.
TextSentencer_T9 642-717 Sentence denotes Of these MC antibodies, 14, 6 and 1 were IgG1, IgG2a and IgM, respectively.
TextSentencer_T9 642-717 Sentence denotes Of these MC antibodies, 14, 6 and 1 were IgG1, IgG2a and IgM, respectively.
TextSentencer_T10 718-784 Sentence denotes All of the MC antibodies contained light chains of the kappa type.
TextSentencer_T10 718-784 Sentence denotes All of the MC antibodies contained light chains of the kappa type.
TextSentencer_T10 718-784 Sentence denotes All of the MC antibodies contained light chains of the kappa type.
TextSentencer_T11 785-885 Sentence denotes Of these MC antibodies, 8 were found to have neutralization (NT) activity against the T0-163 strain.
TextSentencer_T11 785-885 Sentence denotes Of these MC antibodies, 8 were found to have neutralization (NT) activity against the T0-163 strain.
TextSentencer_T11 785-885 Sentence denotes Of these MC antibodies, 8 were found to have neutralization (NT) activity against the T0-163 strain.
TextSentencer_T12 886-1082 Sentence denotes Comparison of 7 strains of TGE virus by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences.
TextSentencer_T12 886-1082 Sentence denotes Comparison of 7 strains of TGE virus by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences.
TextSentencer_T12 886-1082 Sentence denotes Comparison of 7 strains of TGE virus by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences.
TextSentencer_T13 1083-1212 Sentence denotes These results suggest that there may be at least 6 different epitopes involved in NT reaction on the virion of the T0-163 strain.
TextSentencer_T13 1083-1212 Sentence denotes These results suggest that there may be at least 6 different epitopes involved in NT reaction on the virion of the T0-163 strain.
TextSentencer_T13 1083-1212 Sentence denotes These results suggest that there may be at least 6 different epitopes involved in NT reaction on the virion of the T0-163 strain.
TextSentencer_T14 1213-1272 Sentence denotes This notion was confirmed by the competitive binding assay.
TextSentencer_T14 1213-1272 Sentence denotes This notion was confirmed by the competitive binding assay.
TextSentencer_T14 1213-1272 Sentence denotes This notion was confirmed by the competitive binding assay.
TextSentencer_T15 1274-1403 Sentence denotes Transmissible gastroenteritis (TGE) is a highly infectious viral disease of pigs, causing a high mortality rate in young piglets.
TextSentencer_T15 1274-1403 Sentence denotes Transmissible gastroenteritis (TGE) is a highly infectious viral disease of pigs, causing a high mortality rate in young piglets.
TextSentencer_T15 1274-1403 Sentence denotes Transmissible gastroenteritis (TGE) is a highly infectious viral disease of pigs, causing a high mortality rate in young piglets.
TextSentencer_T16 1404-1553 Sentence denotes Minor strain differences between TGE viruses have been shown by neutralization tests with polyclonal antibodies (Harada et al., 1967; Kemeny, 1976) .
TextSentencer_T16 1404-1553 Sentence denotes Minor strain differences between TGE viruses have been shown by neutralization tests with polyclonal antibodies (Harada et al., 1967; Kemeny, 1976) .
TextSentencer_T16 1404-1553 Sentence denotes Minor strain differences between TGE viruses have been shown by neutralization tests with polyclonal antibodies (Harada et al., 1967; Kemeny, 1976) .
TextSentencer_T17 1554-1827 Sentence denotes Monoclonal (MC) antibodies have proved an extremely useful addition to the field of immunology (KShler and Milstein, 1975; Blann, 1979 ) : since they react with only one antigenic determinant (epitope) they are ideal reagents for studying antigenic structure and variation.
TextSentencer_T17 1554-1827 Sentence denotes Monoclonal (MC) antibodies have proved an extremely useful addition to the field of immunology (KShler and Milstein, 1975; Blann, 1979 ) : since they react with only one antigenic determinant (epitope) they are ideal reagents for studying antigenic structure and variation.
TextSentencer_T17 1554-1827 Sentence denotes Monoclonal (MC) antibodies have proved an extremely useful addition to the field of immunology (KShler and Milstein, 1975; Blann, 1979 ) : since they react with only one antigenic determinant (epitope) they are ideal reagents for studying antigenic structure and variation.
TextSentencer_T18 1828-1952 Sentence denotes In the present study, MC antibodies were raised to TGE virus, strain , and used to examine antigenic variation of the virus.
TextSentencer_T18 1828-1952 Sentence denotes In the present study, MC antibodies were raised to TGE virus, strain , and used to examine antigenic variation of the virus.
TextSentencer_T18 1828-1952 Sentence denotes In the present study, MC antibodies were raised to TGE virus, strain , and used to examine antigenic variation of the virus.
TextSentencer_T19 1953-2210 Sentence denotes The CPK cell line derived from swine kidney ( Komaniwa et al., 1981 ) was grown in Eagle's minimum essential medium (MEM) containing 10% calf serum, 10% tryptose phosphate broth (TPB) (Difco, U.S.A. ), 100 units ml-1 penicillin and 100/~g ml-1 streptomycin.
TextSentencer_T19 1953-2210 Sentence denotes The CPK cell line derived from swine kidney ( Komaniwa et al., 1981 ) was grown in Eagle's minimum essential medium (MEM) containing 10% calf serum, 10% tryptose phosphate broth (TPB) (Difco, U.S.A. ), 100 units ml-1 penicillin and 100/~g ml-1 streptomycin.
TextSentencer_T19 1953-2210 Sentence denotes The CPK cell line derived from swine kidney ( Komaniwa et al., 1981 ) was grown in Eagle's minimum essential medium (MEM) containing 10% calf serum, 10% tryptose phosphate broth (TPB) (Difco, U.S.A. ), 100 units ml-1 penicillin and 100/~g ml-1 streptomycin.
TextSentencer_T20 2211-2314 Sentence denotes The maintenance medium was MEM containing 10% TPB, 0.11% bovine serum albumin and antibiotics as above.
TextSentencer_T20 2211-2314 Sentence denotes The maintenance medium was MEM containing 10% TPB, 0.11% bovine serum albumin and antibiotics as above.
TextSentencer_T20 2211-2314 Sentence denotes The maintenance medium was MEM containing 10% TPB, 0.11% bovine serum albumin and antibiotics as above.
TextSentencer_T21 2315-2437 Sentence denotes Primary swine kidney cells dispersed by trypsin were grown and maintained in the same culture media as used for CPK cells.
TextSentencer_T21 2315-2437 Sentence denotes Primary swine kidney cells dispersed by trypsin were grown and maintained in the same culture media as used for CPK cells.
TextSentencer_T21 2315-2437 Sentence denotes Primary swine kidney cells dispersed by trypsin were grown and maintained in the same culture media as used for CPK cells.
TextSentencer_T22 2438-2666 Sentence denotes The TGE virus strains used were TO-163 (Harada et al., 1967; Furuuchi et al., 1975) , h-5, Kanagawa (Harada et al., 1967) , Purdue (Laude et al., 1981 ) , Ukiha, Shizuoka (Harada et al., 1967) and Miller (Bohl and Cross, 1971 ).
TextSentencer_T22 2438-2666 Sentence denotes The TGE virus strains used were TO-163 (Harada et al., 1967; Furuuchi et al., 1975) , h-5, Kanagawa (Harada et al., 1967) , Purdue (Laude et al., 1981 ) , Ukiha, Shizuoka (Harada et al., 1967) and Miller (Bohl and Cross, 1971 ).
TextSentencer_T22 2438-2666 Sentence denotes The TGE virus strains used were TO-163 (Harada et al., 1967; Furuuchi et al., 1975) , h-5, Kanagawa (Harada et al., 1967) , Purdue (Laude et al., 1981 ) , Ukiha, Shizuoka (Harada et al., 1967) and Miller (Bohl and Cross, 1971 ).
TextSentencer_T23 2667-2791 Sentence denotes The T0-163, Kanagawa, Purdue, Shizuoka and Miller strains were provided by the National Institute of Animal Health of Japan.
TextSentencer_T23 2667-2791 Sentence denotes The T0-163, Kanagawa, Purdue, Shizuoka and Miller strains were provided by the National Institute of Animal Health of Japan.
TextSentencer_T23 2667-2791 Sentence denotes The T0-163, Kanagawa, Purdue, Shizuoka and Miller strains were provided by the National Institute of Animal Health of Japan.
TextSentencer_T24 2792-2949 Sentence denotes The h-5 and Ukiha strains were obtained from commercially available vaccines and had been maintained by serial passage in primary swine kidney cell cultures.
TextSentencer_T24 2792-2949 Sentence denotes The h-5 and Ukiha strains were obtained from commercially available vaccines and had been maintained by serial passage in primary swine kidney cell cultures.
TextSentencer_T24 2792-2949 Sentence denotes The h-5 and Ukiha strains were obtained from commercially available vaccines and had been maintained by serial passage in primary swine kidney cell cultures.
TextSentencer_T25 2950-3050 Sentence denotes After two or three passages in these cultures in our laboratory, the strains were stored at -80 ° C.
TextSentencer_T25 2950-3050 Sentence denotes After two or three passages in these cultures in our laboratory, the strains were stored at -80 ° C.
TextSentencer_T25 2950-3050 Sentence denotes After two or three passages in these cultures in our laboratory, the strains were stored at -80 ° C.
TextSentencer_T26 3051-3183 Sentence denotes Infectivity assay was carried out in microplate cultures of CPK cells by the method described previously (Yamagishi et al., 1981 ) .
TextSentencer_T26 3051-3183 Sentence denotes Infectivity assay was carried out in microplate cultures of CPK cells by the method described previously (Yamagishi et al., 1981 ) .
TextSentencer_T26 3051-3183 Sentence denotes Infectivity assay was carried out in microplate cultures of CPK cells by the method described previously (Yamagishi et al., 1981 ) .
TextSentencer_T27 3184-3255 Sentence denotes The titer was expressed in 50% tissue culture infectious dose (TCID5o).
TextSentencer_T27 3184-3255 Sentence denotes The titer was expressed in 50% tissue culture infectious dose (TCID5o).
TextSentencer_T27 3184-3255 Sentence denotes The titer was expressed in 50% tissue culture infectious dose (TCID5o).
TextSentencer_T28 3256-3331 Sentence denotes The antiserum was prepared in specific pathogen-free pigs, 2 months of age.
TextSentencer_T28 3256-3331 Sentence denotes The antiserum was prepared in specific pathogen-free pigs, 2 months of age.
TextSentencer_T28 3256-3331 Sentence denotes The antiserum was prepared in specific pathogen-free pigs, 2 months of age.
TextSentencer_T29 3332-3421 Sentence denotes The animals received two intramuscular doses of 108 TCID5o each at intervals of 2 months.
TextSentencer_T29 3332-3421 Sentence denotes The animals received two intramuscular doses of 108 TCID5o each at intervals of 2 months.
TextSentencer_T29 3332-3421 Sentence denotes The animals received two intramuscular doses of 108 TCID5o each at intervals of 2 months.
TextSentencer_T30 3422-3478 Sentence denotes Serum was obtained 10 days after the second inoculation.
TextSentencer_T30 3422-3478 Sentence denotes Serum was obtained 10 days after the second inoculation.
TextSentencer_T30 3422-3478 Sentence denotes Serum was obtained 10 days after the second inoculation.
TextSentencer_T31 3479-3554 Sentence denotes The antigen was prepared with the T0-163 strain grown in CPK cell cultures.
TextSentencer_T31 3479-3554 Sentence denotes The antigen was prepared with the T0-163 strain grown in CPK cell cultures.
TextSentencer_T31 3479-3554 Sentence denotes The antigen was prepared with the T0-163 strain grown in CPK cell cultures.
TextSentencer_T32 3555-3776 Sentence denotes Infectious culture fluid concentrated about 10-fold by ammonium sulfate precipitation was layered onto a discontinuous sucrose density gradient (20-60%) in a SW 28 rotor (Beckman) and centrifuged at 27 000 r.p.m, for 2 h.
TextSentencer_T32 3555-3776 Sentence denotes Infectious culture fluid concentrated about 10-fold by ammonium sulfate precipitation was layered onto a discontinuous sucrose density gradient (20-60%) in a SW 28 rotor (Beckman) and centrifuged at 27 000 r.p.m, for 2 h.
TextSentencer_T32 3555-3776 Sentence denotes Infectious culture fluid concentrated about 10-fold by ammonium sulfate precipitation was layered onto a discontinuous sucrose density gradient (20-60%) in a SW 28 rotor (Beckman) and centrifuged at 27 000 r.p.m, for 2 h.
TextSentencer_T33 3777-3920 Sentence denotes The virus bands formed were collected, mixed with an equal volume of fluorocarbon (Wako Pure Chemicals, Japan) and vigorously shaken for 2 min.
TextSentencer_T33 3777-3920 Sentence denotes The virus bands formed were collected, mixed with an equal volume of fluorocarbon (Wako Pure Chemicals, Japan) and vigorously shaken for 2 min.
TextSentencer_T33 3777-3920 Sentence denotes The virus bands formed were collected, mixed with an equal volume of fluorocarbon (Wako Pure Chemicals, Japan) and vigorously shaken for 2 min.
TextSentencer_T34 3921-4091 Sentence denotes 117 The water phase was separated by centrifugation and layered onto a linear sucrose density gradient (20-60%) in a SW 28 rotor and centrifuged at 27 000 r.p.m, for 4 h.
TextSentencer_T34 3921-4091 Sentence denotes 117 The water phase was separated by centrifugation and layered onto a linear sucrose density gradient (20-60%) in a SW 28 rotor and centrifuged at 27 000 r.p.m, for 4 h.
TextSentencer_T34 3921-4091 Sentence denotes 117 The water phase was separated by centrifugation and layered onto a linear sucrose density gradient (20-60%) in a SW 28 rotor and centrifuged at 27 000 r.p.m, for 4 h.
TextSentencer_T35 4092-4185 Sentence denotes Fractions with densities of 1.18-1.20 g m1-1 were pooled and centrifuged at 77 000Xg for 1 h.
TextSentencer_T35 4092-4185 Sentence denotes Fractions with densities of 1.18-1.20 g m1-1 were pooled and centrifuged at 77 000Xg for 1 h.
TextSentencer_T35 4092-4185 Sentence denotes Fractions with densities of 1.18-1.20 g m1-1 were pooled and centrifuged at 77 000Xg for 1 h.
TextSentencer_T36 4186-4299 Sentence denotes The pellets were resuspended in a 1/500 volume of NTE buffer (0.1 M NaCl, 0.01 M Tris-HC1, pH 7.4, 0.001 M EDTA).
TextSentencer_T36 4186-4299 Sentence denotes The pellets were resuspended in a 1/500 volume of NTE buffer (0.1 M NaCl, 0.01 M Tris-HC1, pH 7.4, 0.001 M EDTA).
TextSentencer_T36 4186-4299 Sentence denotes The pellets were resuspended in a 1/500 volume of NTE buffer (0.1 M NaCl, 0.01 M Tris-HC1, pH 7.4, 0.001 M EDTA).
TextSentencer_T37 4300-4464 Sentence denotes The suspension was treated with 1% Triton X-100 for 1 h at 4 °C and dialyzed against phosphate buffered saline (PBS) (0.1 M NaCl, 0.01 M phosphate buffer, pH 7.2 ).
TextSentencer_T37 4300-4464 Sentence denotes The suspension was treated with 1% Triton X-100 for 1 h at 4 °C and dialyzed against phosphate buffered saline (PBS) (0.1 M NaCl, 0.01 M phosphate buffer, pH 7.2 ).
TextSentencer_T37 4300-4464 Sentence denotes The suspension was treated with 1% Triton X-100 for 1 h at 4 °C and dialyzed against phosphate buffered saline (PBS) (0.1 M NaCl, 0.01 M phosphate buffer, pH 7.2 ).
TextSentencer_T38 4465-4570 Sentence denotes The resulting material was used for mouse immunization and enzyme-linked immunosorbent assay (see below).
TextSentencer_T38 4465-4570 Sentence denotes The resulting material was used for mouse immunization and enzyme-linked immunosorbent assay (see below).
TextSentencer_T38 4465-4570 Sentence denotes The resulting material was used for mouse immunization and enzyme-linked immunosorbent assay (see below).
TextSentencer_T39 4571-4739 Sentence denotes BALB/c mice, about 5 weeks of age, were inoculated intraperitoneally with a mixture of 10 pg of the viral antigen prepared as above and 109 cells of pertussis adjuvant.
TextSentencer_T39 4571-4739 Sentence denotes BALB/c mice, about 5 weeks of age, were inoculated intraperitoneally with a mixture of 10 pg of the viral antigen prepared as above and 109 cells of pertussis adjuvant.
TextSentencer_T39 4571-4739 Sentence denotes BALB/c mice, about 5 weeks of age, were inoculated intraperitoneally with a mixture of 10 pg of the viral antigen prepared as above and 109 cells of pertussis adjuvant.
TextSentencer_T40 4740-4890 Sentence denotes Four or 6 weeks later the mice received an intravenous booster dose of 10 pg of viral antigen, and spleen cells were obtained for fusion 3 days later.
TextSentencer_T40 4740-4890 Sentence denotes Four or 6 weeks later the mice received an intravenous booster dose of 10 pg of viral antigen, and spleen cells were obtained for fusion 3 days later.
TextSentencer_T40 4740-4890 Sentence denotes Four or 6 weeks later the mice received an intravenous booster dose of 10 pg of viral antigen, and spleen cells were obtained for fusion 3 days later.
TextSentencer_T41 4891-5001 Sentence denotes The fusion was carried out using essentially the same method as that described by KShler and Milstein (1975) .
TextSentencer_T41 4891-5001 Sentence denotes The fusion was carried out using essentially the same method as that described by KShler and Milstein (1975) .
TextSentencer_T41 4891-5001 Sentence denotes The fusion was carried out using essentially the same method as that described by KShler and Milstein (1975) .
TextSentencer_T42 5002-5160 Sentence denotes Polyethyleneglycol-1000 ( Wako Pure Chemicals, Japan) was used as a fusing agent and the ratio of mouse spleen cells and mouse myeloma cells (SP2/O) was 10:1.
TextSentencer_T42 5002-5160 Sentence denotes Polyethyleneglycol-1000 ( Wako Pure Chemicals, Japan) was used as a fusing agent and the ratio of mouse spleen cells and mouse myeloma cells (SP2/O) was 10:1.
TextSentencer_T42 5002-5160 Sentence denotes Polyethyleneglycol-1000 ( Wako Pure Chemicals, Japan) was used as a fusing agent and the ratio of mouse spleen cells and mouse myeloma cells (SP2/O) was 10:1.
TextSentencer_T43 5161-5269 Sentence denotes The selective medium contained hypoxanthine (10 -4 M), aminopterin (4×10 -7 M) and thymidine (1.6 X 10-~ M).
TextSentencer_T43 5161-5269 Sentence denotes The selective medium contained hypoxanthine (10 -4 M), aminopterin (4×10 -7 M) and thymidine (1.6 X 10-~ M).
TextSentencer_T43 5161-5269 Sentence denotes The selective medium contained hypoxanthine (10 -4 M), aminopterin (4×10 -7 M) and thymidine (1.6 X 10-~ M).
TextSentencer_T44 5270-5520 Sentence denotes The fused cells at a concentration ef 3.5 X 106 spleen cells per ml were dispensed in 100 ttl volumes into wells of 96-well, flat-bottomed microplates (Corning Glass Works, Corning, NY) and incubated at 37 ° C in a humid atmosphere containing 5% C02.
TextSentencer_T44 5270-5520 Sentence denotes The fused cells at a concentration ef 3.5 X 106 spleen cells per ml were dispensed in 100 ttl volumes into wells of 96-well, flat-bottomed microplates (Corning Glass Works, Corning, NY) and incubated at 37 ° C in a humid atmosphere containing 5% C02.
TextSentencer_T44 5270-5520 Sentence denotes The fused cells at a concentration ef 3.5 X 106 spleen cells per ml were dispensed in 100 ttl volumes into wells of 96-well, flat-bottomed microplates (Corning Glass Works, Corning, NY) and incubated at 37 ° C in a humid atmosphere containing 5% C02.
TextSentencer_T45 5521-5712 Sentence denotes After incubation for 2 weeks, the wells were examined and those which contained hybridoma cultures were tested for TGE specific antibody by an enzyme-linked immunosorbent assay ( see below ).
TextSentencer_T45 5521-5712 Sentence denotes After incubation for 2 weeks, the wells were examined and those which contained hybridoma cultures were tested for TGE specific antibody by an enzyme-linked immunosorbent assay ( see below ).
TextSentencer_T45 5521-5712 Sentence denotes After incubation for 2 weeks, the wells were examined and those which contained hybridoma cultures were tested for TGE specific antibody by an enzyme-linked immunosorbent assay ( see below ).
TextSentencer_T46 5713-5914 Sentence denotes The colonies in antibody positive wells were passaged in 24-well multiplates ( Corning Glass Works, Corning, NY) and incubated in medium containing hypoxanthine (10-4 M ) and thymidine (1.6 X 10-~ M ).
TextSentencer_T46 5713-5914 Sentence denotes The colonies in antibody positive wells were passaged in 24-well multiplates ( Corning Glass Works, Corning, NY) and incubated in medium containing hypoxanthine (10-4 M ) and thymidine (1.6 X 10-~ M ).
TextSentencer_T46 5713-5914 Sentence denotes The colonies in antibody positive wells were passaged in 24-well multiplates ( Corning Glass Works, Corning, NY) and incubated in medium containing hypoxanthine (10-4 M ) and thymidine (1.6 X 10-~ M ).
TextSentencer_T47 5915-5972 Sentence denotes The cells were then cloned twice by the soft agar method.
TextSentencer_T47 5915-5972 Sentence denotes The cells were then cloned twice by the soft agar method.
TextSentencer_T47 5915-5972 Sentence denotes The cells were then cloned twice by the soft agar method.
TextSentencer_T48 5973-6165 Sentence denotes BALB/c mice were injected intraperitoneally with 0.5 ml of pristane (2,6,10,14-tetramethylpentadecane) { Aldrich, U.S.A.) 14 days before intraperitoneal inoculation of 2 X 106 hybridoma cells.
TextSentencer_T48 5973-6165 Sentence denotes BALB/c mice were injected intraperitoneally with 0.5 ml of pristane (2,6,10,14-tetramethylpentadecane) { Aldrich, U.S.A.) 14 days before intraperitoneal inoculation of 2 X 106 hybridoma cells.
TextSentencer_T48 5973-6165 Sentence denotes BALB/c mice were injected intraperitoneally with 0.5 ml of pristane (2,6,10,14-tetramethylpentadecane) { Aldrich, U.S.A.) 14 days before intraperitoneal inoculation of 2 X 106 hybridoma cells.
TextSentencer_T49 6166-6276 Sentence denotes Ten days later the ascitic fluids were collected, clarified by centrifugation and stored at -80 ° C until use.
TextSentencer_T49 6166-6276 Sentence denotes Ten days later the ascitic fluids were collected, clarified by centrifugation and stored at -80 ° C until use.
TextSentencer_T49 6166-6276 Sentence denotes Ten days later the ascitic fluids were collected, clarified by centrifugation and stored at -80 ° C until use.
TextSentencer_T50 6277-6522 Sentence denotes The supernatant fluids of antibody-secreting hybridoma cultures were concentrated 10-fold by 50% saturation of ammonium sulfate and used for deter-mination of antibody class and subclass by double diffusion in a 1% agar gel containing 0.1% NAN3.
TextSentencer_T50 6277-6522 Sentence denotes The supernatant fluids of antibody-secreting hybridoma cultures were concentrated 10-fold by 50% saturation of ammonium sulfate and used for deter-mination of antibody class and subclass by double diffusion in a 1% agar gel containing 0.1% NAN3.
TextSentencer_T50 6277-6522 Sentence denotes The supernatant fluids of antibody-secreting hybridoma cultures were concentrated 10-fold by 50% saturation of ammonium sulfate and used for deter-mination of antibody class and subclass by double diffusion in a 1% agar gel containing 0.1% NAN3.
TextSentencer_T51 6523-6745 Sentence denotes Rabbit antisera against mouse immunoglobulins, IgG1, IgG2a, IgG2b, IgG3, IgM and IgA, and kappa and lambda chains (Miles Laboratories, U.S.A. ) were placed in the center wells and test samples were added to adjacent wells.
TextSentencer_T51 6523-6745 Sentence denotes Rabbit antisera against mouse immunoglobulins, IgG1, IgG2a, IgG2b, IgG3, IgM and IgA, and kappa and lambda chains (Miles Laboratories, U.S.A. ) were placed in the center wells and test samples were added to adjacent wells.
TextSentencer_T51 6523-6745 Sentence denotes Rabbit antisera against mouse immunoglobulins, IgG1, IgG2a, IgG2b, IgG3, IgM and IgA, and kappa and lambda chains (Miles Laboratories, U.S.A. ) were placed in the center wells and test samples were added to adjacent wells.
TextSentencer_T52 6746-6826 Sentence denotes The plates were incubated overnight at room temperature in a humidified chamber.
TextSentencer_T52 6746-6826 Sentence denotes The plates were incubated overnight at room temperature in a humidified chamber.
TextSentencer_T52 6746-6826 Sentence denotes The plates were incubated overnight at room temperature in a humidified chamber.
TextSentencer_T53 6827-7015 Sentence denotes Virus antigen (see above) was appropriately diluted with carbonate buffer (0.05 M, pH 9.6) and delivered in 100 #l volumes into wells of 96-well, flatbottomed Microelisa plates ( Dynatech.
TextSentencer_T53 6827-7015 Sentence denotes Virus antigen (see above) was appropriately diluted with carbonate buffer (0.05 M, pH 9.6) and delivered in 100 #l volumes into wells of 96-well, flatbottomed Microelisa plates ( Dynatech.
TextSentencer_T53 6827-7015 Sentence denotes Virus antigen (see above) was appropriately diluted with carbonate buffer (0.05 M, pH 9.6) and delivered in 100 #l volumes into wells of 96-well, flatbottomed Microelisa plates ( Dynatech.
TextSentencer_T54 7016-7031 Sentence denotes Lab., U.S.A. ).
TextSentencer_T54 7016-7031 Sentence denotes Lab., U.S.A. ).
TextSentencer_T54 7016-7031 Sentence denotes Lab., U.S.A. ).
TextSentencer_T55 7032-7197 Sentence denotes The plates were allowed to stand overnight at 4 ° C, washed with 0.85% NaCl solution containing 0.02% Tween-20, and 100 pl of the test sample was added to each well.
TextSentencer_T55 7032-7197 Sentence denotes The plates were allowed to stand overnight at 4 ° C, washed with 0.85% NaCl solution containing 0.02% Tween-20, and 100 pl of the test sample was added to each well.
TextSentencer_T55 7032-7197 Sentence denotes The plates were allowed to stand overnight at 4 ° C, washed with 0.85% NaCl solution containing 0.02% Tween-20, and 100 pl of the test sample was added to each well.
TextSentencer_T56 7198-7440 Sentence denotes Horseradish peroxidase-conjugated rabbit antibodies against mouse immunoglobulins were diluted to the optimal concentration with PBS containing 10% calf serum and 0.05% Tween-20 and 100 pl of the dilution was added to each well of the plates.
TextSentencer_T56 7198-7440 Sentence denotes Horseradish peroxidase-conjugated rabbit antibodies against mouse immunoglobulins were diluted to the optimal concentration with PBS containing 10% calf serum and 0.05% Tween-20 and 100 pl of the dilution was added to each well of the plates.
TextSentencer_T56 7198-7440 Sentence denotes Horseradish peroxidase-conjugated rabbit antibodies against mouse immunoglobulins were diluted to the optimal concentration with PBS containing 10% calf serum and 0.05% Tween-20 and 100 pl of the dilution was added to each well of the plates.
TextSentencer_T57 7441-7576 Sentence denotes After incubation at 37°C for 30 min, each well received 100 pl of substrate solution and incubated at 25 ° C for 20 min in a dark room.
TextSentencer_T57 7441-7576 Sentence denotes After incubation at 37°C for 30 min, each well received 100 pl of substrate solution and incubated at 25 ° C for 20 min in a dark room.
TextSentencer_T57 7441-7576 Sentence denotes After incubation at 37°C for 30 min, each well received 100 pl of substrate solution and incubated at 25 ° C for 20 min in a dark room.
TextSentencer_T58 7577-7782 Sentence denotes The substrate solution was prepared by dissolving O-phenylenediamine dihydrochloride at a concentration of 0.4 mg m1-1 in 0.1 M citric acid-0.2 M Na2HP04 buffer (pH 4.8) and adding 0.2 ~l ml-1 of 30% H202.
TextSentencer_T58 7577-7782 Sentence denotes The substrate solution was prepared by dissolving O-phenylenediamine dihydrochloride at a concentration of 0.4 mg m1-1 in 0.1 M citric acid-0.2 M Na2HP04 buffer (pH 4.8) and adding 0.2 ~l ml-1 of 30% H202.
TextSentencer_T58 7577-7782 Sentence denotes The substrate solution was prepared by dissolving O-phenylenediamine dihydrochloride at a concentration of 0.4 mg m1-1 in 0.1 M citric acid-0.2 M Na2HP04 buffer (pH 4.8) and adding 0.2 ~l ml-1 of 30% H202.
TextSentencer_T59 7783-7922 Sentence denotes After completion of the incubation, the reaction was stopped with 3 N H2SO4 solution and the optical density (OD) at 492 nm was determined.
TextSentencer_T59 7783-7922 Sentence denotes After completion of the incubation, the reaction was stopped with 3 N H2SO4 solution and the optical density (OD) at 492 nm was determined.
TextSentencer_T59 7783-7922 Sentence denotes After completion of the incubation, the reaction was stopped with 3 N H2SO4 solution and the optical density (OD) at 492 nm was determined.
TextSentencer_T60 7923-8076 Sentence denotes NT tests were carried out by the antibody dilution method in CPK cell cultures prepared in flat-bottomed microplates (Corning Glass Works, Corning, NY ).
TextSentencer_T60 7923-8076 Sentence denotes NT tests were carried out by the antibody dilution method in CPK cell cultures prepared in flat-bottomed microplates (Corning Glass Works, Corning, NY ).
TextSentencer_T60 7923-8076 Sentence denotes NT tests were carried out by the antibody dilution method in CPK cell cultures prepared in flat-bottomed microplates (Corning Glass Works, Corning, NY ).
TextSentencer_T61 8077-8271 Sentence denotes 25/A of each of serial 2-fold dilutions of the antibody inactivated at 56 ° C for 30 min were mixed with 25 }tl of maintenance medium containing 200 TCIDso of virus in wells of a transfer plate.
TextSentencer_T61 8077-8271 Sentence denotes 25/A of each of serial 2-fold dilutions of the antibody inactivated at 56 ° C for 30 min were mixed with 25 }tl of maintenance medium containing 200 TCIDso of virus in wells of a transfer plate.
TextSentencer_T61 8077-8271 Sentence denotes 25/A of each of serial 2-fold dilutions of the antibody inactivated at 56 ° C for 30 min were mixed with 25 }tl of maintenance medium containing 200 TCIDso of virus in wells of a transfer plate.
TextSentencer_T62 8272-8324 Sentence denotes Four wells were employed for each antibody dilution.
TextSentencer_T62 8272-8324 Sentence denotes Four wells were employed for each antibody dilution.
TextSentencer_T62 8272-8324 Sentence denotes Four wells were employed for each antibody dilution.
TextSentencer_T63 8325-8525 Sentence denotes The virus-antibody mixtures were incubated at 37°C for 60 min, transferred into wells of a microplate containing CPK cell cultures, and incubated in an atmosphere of 5% CO2 in air at 37 °C for 5 days.
TextSentencer_T63 8325-8525 Sentence denotes The virus-antibody mixtures were incubated at 37°C for 60 min, transferred into wells of a microplate containing CPK cell cultures, and incubated in an atmosphere of 5% CO2 in air at 37 °C for 5 days.
TextSentencer_T63 8325-8525 Sentence denotes The virus-antibody mixtures were incubated at 37°C for 60 min, transferred into wells of a microplate containing CPK cell cultures, and incubated in an atmosphere of 5% CO2 in air at 37 °C for 5 days.
TextSentencer_T64 8526-8653 Sentence denotes The antibody titer was expressed as the reciprocal of the antibody dilution showing 50% NT calculated by the method of K~irber.
TextSentencer_T64 8526-8653 Sentence denotes The antibody titer was expressed as the reciprocal of the antibody dilution showing 50% NT calculated by the method of K~irber.
TextSentencer_T64 8526-8653 Sentence denotes The antibody titer was expressed as the reciprocal of the antibody dilution showing 50% NT calculated by the method of K~irber.
TextSentencer_T65 8654-8715 Sentence denotes This was prepared by the method of Nakane and Kawaoi (1974) .
TextSentencer_T65 8654-8715 Sentence denotes This was prepared by the method of Nakane and Kawaoi (1974) .
TextSentencer_T65 8654-8715 Sentence denotes This was prepared by the method of Nakane and Kawaoi (1974) .
TextSentencer_T66 8716-8827 Sentence denotes MC antibodies from ascitic fluid were purified by 3 cycles of precipitation with (NH4) 2SO4 at 35 % saturation.
TextSentencer_T66 8716-8827 Sentence denotes MC antibodies from ascitic fluid were purified by 3 cycles of precipitation with (NH4) 2SO4 at 35 % saturation.
TextSentencer_T66 8716-8827 Sentence denotes MC antibodies from ascitic fluid were purified by 3 cycles of precipitation with (NH4) 2SO4 at 35 % saturation.
TextSentencer_T67 8828-9003 Sentence denotes Ten mg of the purified MC antibody was mixed 119 with 4 mg of horseradish peroxidase oxidized with 0.1 M NaIO4 in 0.01 M carbonate buffer (pH 9.6) at room temperature for 2 h.
TextSentencer_T67 8828-9003 Sentence denotes Ten mg of the purified MC antibody was mixed 119 with 4 mg of horseradish peroxidase oxidized with 0.1 M NaIO4 in 0.01 M carbonate buffer (pH 9.6) at room temperature for 2 h.
TextSentencer_T67 8828-9003 Sentence denotes Ten mg of the purified MC antibody was mixed 119 with 4 mg of horseradish peroxidase oxidized with 0.1 M NaIO4 in 0.01 M carbonate buffer (pH 9.6) at room temperature for 2 h.
TextSentencer_T68 9004-9127 Sentence denotes After addition of 4 mg NaBH4 solution and incubation at 4 °C for 2 h, the mixture was dialyzed against 0.01 M PBS (pH 7.2).
TextSentencer_T68 9004-9127 Sentence denotes After addition of 4 mg NaBH4 solution and incubation at 4 °C for 2 h, the mixture was dialyzed against 0.01 M PBS (pH 7.2).
TextSentencer_T68 9004-9127 Sentence denotes After addition of 4 mg NaBH4 solution and incubation at 4 °C for 2 h, the mixture was dialyzed against 0.01 M PBS (pH 7.2).
TextSentencer_T69 9128-9242 Sentence denotes The enzyme conjugated antibody was separated from the enzyme and unconjugated antibody on a Sephadex G-200 column.
TextSentencer_T69 9128-9242 Sentence denotes The enzyme conjugated antibody was separated from the enzyme and unconjugated antibody on a Sephadex G-200 column.
TextSentencer_T69 9128-9242 Sentence denotes The enzyme conjugated antibody was separated from the enzyme and unconjugated antibody on a Sephadex G-200 column.
TextSentencer_T70 9243-9318 Sentence denotes This was carried out by a modified ELISA (Kimura-Kuroda and Yasui, 1983 ) .
TextSentencer_T70 9243-9318 Sentence denotes This was carried out by a modified ELISA (Kimura-Kuroda and Yasui, 1983 ) .
TextSentencer_T70 9243-9318 Sentence denotes This was carried out by a modified ELISA (Kimura-Kuroda and Yasui, 1983 ) .
TextSentencer_T71 9319-9543 Sentence denotes One hundred zl of serial 10-fold dilutions of each competing MC antibody from ascitic fluid were added to wells of a flat-bottomed Microelisa plate coated with virus antigen ( see above ), and incubated at 37 ° C for 60 min.
TextSentencer_T71 9319-9543 Sentence denotes One hundred zl of serial 10-fold dilutions of each competing MC antibody from ascitic fluid were added to wells of a flat-bottomed Microelisa plate coated with virus antigen ( see above ), and incubated at 37 ° C for 60 min.
TextSentencer_T71 9319-9543 Sentence denotes One hundred zl of serial 10-fold dilutions of each competing MC antibody from ascitic fluid were added to wells of a flat-bottomed Microelisa plate coated with virus antigen ( see above ), and incubated at 37 ° C for 60 min.
TextSentencer_T72 9544-9704 Sentence denotes After three washings with 0.85% NaC1 solution containing 0.02% Tween-20, 100 #l of peroxidase-labeled MC antibody (see above) diluted to an OD of 1.0 was added.
TextSentencer_T72 9544-9704 Sentence denotes After three washings with 0.85% NaC1 solution containing 0.02% Tween-20, 100 #l of peroxidase-labeled MC antibody (see above) diluted to an OD of 1.0 was added.
TextSentencer_T72 9544-9704 Sentence denotes After three washings with 0.85% NaC1 solution containing 0.02% Tween-20, 100 #l of peroxidase-labeled MC antibody (see above) diluted to an OD of 1.0 was added.
TextSentencer_T73 9705-9817 Sentence denotes The competitive binding was estimated from the ODs in the presence or absence of unlabeled competing antibodies.
TextSentencer_T73 9705-9817 Sentence denotes The competitive binding was estimated from the ODs in the presence or absence of unlabeled competing antibodies.
TextSentencer_T73 9705-9817 Sentence denotes The competitive binding was estimated from the ODs in the presence or absence of unlabeled competing antibodies.
TextSentencer_T74 9818-9878 Sentence denotes The percentage of competition was determined by the formula:
TextSentencer_T74 9818-9878 Sentence denotes The percentage of competition was determined by the formula:
TextSentencer_T74 9818-9878 Sentence denotes The percentage of competition was determined by the formula:
TextSentencer_T75 9879-10074 Sentence denotes 100 (A -n) / (A -B ), where A is the OD in the absence of competing antibody, B is the OD in the presence of homologous antibody (10 t ELISA units), and n is the OD in the presence of competitor.
TextSentencer_T75 9879-10074 Sentence denotes 100 (A -n) / (A -B ), where A is the OD in the absence of competing antibody, B is the OD in the presence of homologous antibody (10 t ELISA units), and n is the OD in the presence of competitor.
TextSentencer_T75 9879-10074 Sentence denotes 100 (A -n) / (A -B ), where A is the OD in the absence of competing antibody, B is the OD in the presence of homologous antibody (10 t ELISA units), and n is the OD in the presence of competitor.
TextSentencer_T76 10075-10166 Sentence denotes In two fusion experiments, hybridoma cultures were obtained in 152 of 632 inoculated wells.
TextSentencer_T76 10075-10166 Sentence denotes In two fusion experiments, hybridoma cultures were obtained in 152 of 632 inoculated wells.
TextSentencer_T76 10075-10166 Sentence denotes In two fusion experiments, hybridoma cultures were obtained in 152 of 632 inoculated wells.
TextSentencer_T77 10167-10284 Sentence denotes Antibody-positive colonies were cloned twice in soft agar, and 21 clones secreting MC antibody were finally obtained.
TextSentencer_T77 10167-10284 Sentence denotes Antibody-positive colonies were cloned twice in soft agar, and 21 clones secreting MC antibody were finally obtained.
TextSentencer_T77 10167-10284 Sentence denotes Antibody-positive colonies were cloned twice in soft agar, and 21 clones secreting MC antibody were finally obtained.
TextSentencer_T78 10285-10379 Sentence denotes Of these cloned cells, 14, six and one produced IgG1, IgG2a, and IgM antibodies, respectively.
TextSentencer_T78 10285-10379 Sentence denotes Of these cloned cells, 14, six and one produced IgG1, IgG2a, and IgM antibodies, respectively.
TextSentencer_T78 10285-10379 Sentence denotes Of these cloned cells, 14, six and one produced IgG1, IgG2a, and IgM antibodies, respectively.
TextSentencer_T79 10380-10454 Sentence denotes All of these MC antibodies had light chains of the kappa type (Table I ) .
TextSentencer_T79 10380-10454 Sentence denotes All of these MC antibodies had light chains of the kappa type (Table I ) .
TextSentencer_T79 10380-10454 Sentence denotes All of these MC antibodies had light chains of the kappa type (Table I ) .
TextSentencer_T80 10455-10666 Sentence denotes The reactivity of the MC antibodies to the TO-163 strain of TGE virus was examined by NT and ELISA using the mouse ascitic fluid and the culture fluid concentration 10-fold by 50% saturation of ammonium sulfate.
TextSentencer_T80 10455-10666 Sentence denotes The reactivity of the MC antibodies to the TO-163 strain of TGE virus was examined by NT and ELISA using the mouse ascitic fluid and the culture fluid concentration 10-fold by 50% saturation of ammonium sulfate.
TextSentencer_T80 10455-10666 Sentence denotes The reactivity of the MC antibodies to the TO-163 strain of TGE virus was examined by NT and ELISA using the mouse ascitic fluid and the culture fluid concentration 10-fold by 50% saturation of ammonium sulfate.
TextSentencer_T81 10667-10735 Sentence denotes As shown in Table I , eight of the 21 MC antibodies had NT activity.
TextSentencer_T81 10667-10735 Sentence denotes As shown in Table I , eight of the 21 MC antibodies had NT activity.
TextSentencer_T81 10667-10735 Sentence denotes As shown in Table I , eight of the 21 MC antibodies had NT activity.
TextSentencer_T82 10736-11037 Sentence denotes Of these, three antibodies (4-4, 75-4, 66-A) had high antibody titers in both the NT and ELISA tests, and four (55-2, 16-C, 38-A, 39-7) had high ELISA titers, but low NT titers, 10-fold to 100-fold lower than those of 4-4, 75-4 and 66-A MC antibodies, and one (9-C) had rather low ELISA and NT titers.
TextSentencer_T82 10736-11037 Sentence denotes Of these, three antibodies (4-4, 75-4, 66-A) had high antibody titers in both the NT and ELISA tests, and four (55-2, 16-C, 38-A, 39-7) had high ELISA titers, but low NT titers, 10-fold to 100-fold lower than those of 4-4, 75-4 and 66-A MC antibodies, and one (9-C) had rather low ELISA and NT titers.
TextSentencer_T82 10736-11037 Sentence denotes Of these, three antibodies (4-4, 75-4, 66-A) had high antibody titers in both the NT and ELISA tests, and four (55-2, 16-C, 38-A, 39-7) had high ELISA titers, but low NT titers, 10-fold to 100-fold lower than those of 4-4, 75-4 and 66-A MC antibodies, and one (9-C) had rather low ELISA and NT titers.
TextSentencer_T83 11038-11117 Sentence denotes The remaining 13 MC antibodies showed positive ELISA titers but no NT activity.
TextSentencer_T83 11038-11117 Sentence denotes The remaining 13 MC antibodies showed positive ELISA titers but no NT activity.
TextSentencer_T83 11038-11117 Sentence denotes The remaining 13 MC antibodies showed positive ELISA titers but no NT activity.
TextSentencer_T84 11118-11233 Sentence denotes Culture fluid of mouse myeloma cells (SP2/O) concentrated 10-fold by ammonium sulfate was negative in ELISA and NT.
TextSentencer_T84 11118-11233 Sentence denotes Culture fluid of mouse myeloma cells (SP2/O) concentrated 10-fold by ammonium sulfate was negative in ELISA and NT.
TextSentencer_T84 11118-11233 Sentence denotes Culture fluid of mouse myeloma cells (SP2/O) concentrated 10-fold by ammonium sulfate was negative in ELISA and NT.
TextSentencer_T85 11235-11379 Sentence denotes Seven strains of TGE virus were examined for their antigenicity by NT tests using the eight MC antibodies with NT activity to the TO-163 strain.
TextSentencer_T85 11235-11379 Sentence denotes Seven strains of TGE virus were examined for their antigenicity by NT tests using the eight MC antibodies with NT activity to the TO-163 strain.
TextSentencer_T85 11235-11379 Sentence denotes Seven strains of TGE virus were examined for their antigenicity by NT tests using the eight MC antibodies with NT activity to the TO-163 strain.
TextSentencer_T86 11380-11420 Sentence denotes The results are summarized in Table II .
TextSentencer_T86 11380-11420 Sentence denotes The results are summarized in Table II .
TextSentencer_T86 11380-11420 Sentence denotes The results are summarized in Table II .
TextSentencer_T87 11421-11665 Sentence denotes Based on these results the seven virus strains could be classified into four groups: the TO-163, h-5 and Kanagawa strains in Group I, the Purdue strain in Group II, the Ukiha strain in Group III, and the Shizuoka and Miller strains in Group IV.
TextSentencer_T87 11421-11665 Sentence denotes Based on these results the seven virus strains could be classified into four groups: the TO-163, h-5 and Kanagawa strains in Group I, the Purdue strain in Group II, the Ukiha strain in Group III, and the Shizuoka and Miller strains in Group IV.
TextSentencer_T87 11421-11665 Sentence denotes Based on these results the seven virus strains could be classified into four groups: the TO-163, h-5 and Kanagawa strains in Group I, the Purdue strain in Group II, the Ukiha strain in Group III, and the Shizuoka and Miller strains in Group IV.
TextSentencer_T88 11666-11842 Sentence denotes MC antibodies 4-4, 55-2 and 38-A, demonstrated NT activity to all of the virus strains tested, although the 38-A antibody showed low titers compared with those of 4-4 and 55-2.
TextSentencer_T88 11666-11842 Sentence denotes MC antibodies 4-4, 55-2 and 38-A, demonstrated NT activity to all of the virus strains tested, although the 38-A antibody showed low titers compared with those of 4-4 and 55-2.
TextSentencer_T88 11666-11842 Sentence denotes MC antibodies 4-4, 55-2 and 38-A, demonstrated NT activity to all of the virus strains tested, although the 38-A antibody showed low titers compared with those of 4-4 and 55-2.
TextSentencer_T89 11843-12046 Sentence denotes The 16-C antibody neutralized the six virus strain in Groups I, III and IV, but not the Purdue strain of Group II, while the 9-C antibody neutralized all the strains except the Ukiha strain in Group III.
TextSentencer_T89 11843-12046 Sentence denotes The 16-C antibody neutralized the six virus strain in Groups I, III and IV, but not the Purdue strain of Group II, while the 9-C antibody neutralized all the strains except the Ukiha strain in Group III.
TextSentencer_T89 11843-12046 Sentence denotes The 16-C antibody neutralized the six virus strain in Groups I, III and IV, but not the Purdue strain of Group II, while the 9-C antibody neutralized all the strains except the Ukiha strain in Group III.
TextSentencer_T90 12047-12243 Sentence denotes The 66-A and 75-4 antibodies showed high NT activities to the strains of Group I, but low NT activity to the Purdue strain of Group II, and no NT activity to the strains of Group III and Group IV.
TextSentencer_T90 12047-12243 Sentence denotes The 66-A and 75-4 antibodies showed high NT activities to the strains of Group I, but low NT activity to the Purdue strain of Group II, and no NT activity to the strains of Group III and Group IV.
TextSentencer_T90 12047-12243 Sentence denotes The 66-A and 75-4 antibodies showed high NT activities to the strains of Group I, but low NT activity to the Purdue strain of Group II, and no NT activity to the strains of Group III and Group IV.
TextSentencer_T91 12244-12373 Sentence denotes The 39-7 antibody exhibited NT activity only to the Group I strains, but none to the other four strains in Groups II, III and IV.
TextSentencer_T91 12244-12373 Sentence denotes The 39-7 antibody exhibited NT activity only to the Group I strains, but none to the other four strains in Groups II, III and IV.
TextSentencer_T91 12244-12373 Sentence denotes The 39-7 antibody exhibited NT activity only to the Group I strains, but none to the other four strains in Groups II, III and IV.
TextSentencer_T92 12374-12542 Sentence denotes The polyclonal antiserum to the TO-163 strain prepared in a pig showed essentially the same NT titers to all the virus strains and could not differentiate between them.
TextSentencer_T92 12374-12542 Sentence denotes The polyclonal antiserum to the TO-163 strain prepared in a pig showed essentially the same NT titers to all the virus strains and could not differentiate between them.
TextSentencer_T92 12374-12542 Sentence denotes The polyclonal antiserum to the TO-163 strain prepared in a pig showed essentially the same NT titers to all the virus strains and could not differentiate between them.
TextSentencer_T93 12543-12724 Sentence denotes The TO-163 and Shizuoka strains at low and high passage levels in swine kidney cell cultures were tested against the eight MC antibodies with NT activity against the TO-163 strains.
TextSentencer_T93 12543-12724 Sentence denotes The TO-163 and Shizuoka strains at low and high passage levels in swine kidney cell cultures were tested against the eight MC antibodies with NT activity against the TO-163 strains.
TextSentencer_T93 12543-12724 Sentence denotes The TO-163 and Shizuoka strains at low and high passage levels in swine kidney cell cultures were tested against the eight MC antibodies with NT activity against the TO-163 strains.
TextSentencer_T94 12725-12858 Sentence denotes As shown in Table III , the low-passage TO strain showed an NT titer pattern quite different from that of the high-passage TO strain.
TextSentencer_T94 12725-12858 Sentence denotes As shown in Table III , the low-passage TO strain showed an NT titer pattern quite different from that of the high-passage TO strain.
TextSentencer_T94 12725-12858 Sentence denotes As shown in Table III , the low-passage TO strain showed an NT titer pattern quite different from that of the high-passage TO strain.
TextSentencer_T95 12859-13071 Sentence denotes The high-passage TO strain (176 passages) showed the NT titer pattern of Group I, whereas the low-passage TO strain (17 passages) demonstrated an NT titer pattern similar to that of Group IV (Tables II and III) .
TextSentencer_T95 12859-13071 Sentence denotes The high-passage TO strain (176 passages) showed the NT titer pattern of Group I, whereas the low-passage TO strain (17 passages) demonstrated an NT titer pattern similar to that of Group IV (Tables II and III) .
TextSentencer_T95 12859-13071 Sentence denotes The high-passage TO strain (176 passages) showed the NT titer pattern of Group I, whereas the low-passage TO strain (17 passages) demonstrated an NT titer pattern similar to that of Group IV (Tables II and III) .
TextSentencer_T96 13072-13295 Sentence denotes On the other hand, the Shizuoka strain showed no difference in NT titer pattern between the low-passage ( 22 passages) and high-passage virus (163 passages), the NT titer pattern being that of Group IV (Tables II and III) .
TextSentencer_T96 13072-13295 Sentence denotes On the other hand, the Shizuoka strain showed no difference in NT titer pattern between the low-passage ( 22 passages) and high-passage virus (163 passages), the NT titer pattern being that of Group IV (Tables II and III) .
TextSentencer_T96 13072-13295 Sentence denotes On the other hand, the Shizuoka strain showed no difference in NT titer pattern between the low-passage ( 22 passages) and high-passage virus (163 passages), the NT titer pattern being that of Group IV (Tables II and III) .
TextSentencer_T97 13296-13361 Sentence denotes Table IV summarizes the results of the competitive binding assay.
TextSentencer_T97 13296-13361 Sentence denotes Table IV summarizes the results of the competitive binding assay.
TextSentencer_T97 13296-13361 Sentence denotes Table IV summarizes the results of the competitive binding assay.
TextSentencer_T98 13362-13530 Sentence denotes Enzymelabeled 4-4 and 55-2 MC antibodies were blocked completely by either one of these antibodies, and similar results were obtained with MC antibodies, 66-A and 75-4.
TextSentencer_T98 13362-13530 Sentence denotes Enzymelabeled 4-4 and 55-2 MC antibodies were blocked completely by either one of these antibodies, and similar results were obtained with MC antibodies, 66-A and 75-4.
TextSentencer_T98 13362-13530 Sentence denotes Enzymelabeled 4-4 and 55-2 MC antibodies were blocked completely by either one of these antibodies, and similar results were obtained with MC antibodies, 66-A and 75-4.
TextSentencer_T99 13531-13629 Sentence denotes MC antibodies 38-A, 16-C, 9-C and 39-7 completely blocked only homologous enzyme-labeled antibody.
TextSentencer_T99 13531-13629 Sentence denotes MC antibodies 38-A, 16-C, 9-C and 39-7 completely blocked only homologous enzyme-labeled antibody.
TextSentencer_T99 13531-13629 Sentence denotes MC antibodies 38-A, 16-C, 9-C and 39-7 completely blocked only homologous enzyme-labeled antibody.
TextSentencer_T100 13630-13739 Sentence denotes MC antibodies 66-A and 75-4, partially blocked enzyme-labeled antibodies 4-4, 55-2, 38-A, 16-C, 9-C and 39-7.
TextSentencer_T100 13630-13739 Sentence denotes MC antibodies 66-A and 75-4, partially blocked enzyme-labeled antibodies 4-4, 55-2, 38-A, 16-C, 9-C and 39-7.
TextSentencer_T100 13630-13739 Sentence denotes MC antibodies 66-A and 75-4, partially blocked enzyme-labeled antibodies 4-4, 55-2, 38-A, 16-C, 9-C and 39-7.
TextSentencer_T101 13740-13822 Sentence denotes Three major structural proteins have been demonstrated in the virion of TGE virus.
TextSentencer_T101 13740-13822 Sentence denotes Three major structural proteins have been demonstrated in the virion of TGE virus.
TextSentencer_T101 13740-13822 Sentence denotes Three major structural proteins have been demonstrated in the virion of TGE virus.
TextSentencer_T102 13823-13997 Sentence denotes They include phosphorylated nucleocapsid protein (N), glycosylated envelope protein { E1 ) and glycosylated peplomer protein (E2) (Jacobs et al., 1986; Laude et al., 1986 ) .
TextSentencer_T102 13823-13997 Sentence denotes They include phosphorylated nucleocapsid protein (N), glycosylated envelope protein { E1 ) and glycosylated peplomer protein (E2) (Jacobs et al., 1986; Laude et al., 1986 ) .
TextSentencer_T102 13823-13997 Sentence denotes They include phosphorylated nucleocapsid protein (N), glycosylated envelope protein { E1 ) and glycosylated peplomer protein (E2) (Jacobs et al., 1986; Laude et al., 1986 ) .
TextSentencer_T103 13998-14197 Sentence denotes Garwes et al. (1979) reported that glycoprotein VP1 (E2) was the structural component of virus surface projections and that a preparation of virus surface projections produced NT antibody in animals.
TextSentencer_T103 13998-14197 Sentence denotes Garwes et al. (1979) reported that glycoprotein VP1 (E2) was the structural component of virus surface projections and that a preparation of virus surface projections produced NT antibody in animals.
TextSentencer_T103 13998-14197 Sentence denotes Garwes et al. (1979) reported that glycoprotein VP1 (E2) was the structural component of virus surface projections and that a preparation of virus surface projections produced NT antibody in animals.
TextSentencer_T104 14198-14364 Sentence denotes Laude et al. (1986) raised MC antibodies against structural proteins of TGE virus and showed all major NT-mediating determinants to be carried by E2 peplomer protein.
TextSentencer_T104 14198-14364 Sentence denotes Laude et al. (1986) raised MC antibodies against structural proteins of TGE virus and showed all major NT-mediating determinants to be carried by E2 peplomer protein.
TextSentencer_T104 14198-14364 Sentence denotes Laude et al. (1986) raised MC antibodies against structural proteins of TGE virus and showed all major NT-mediating determinants to be carried by E2 peplomer protein.
TextSentencer_T105 14365-14479 Sentence denotes The epitopes recognized by our panel of MC antibodies with NT activity may also be present on E2 peplomer protein.
TextSentencer_T105 14365-14479 Sentence denotes The epitopes recognized by our panel of MC antibodies with NT activity may also be present on E2 peplomer protein.
TextSentencer_T105 14365-14479 Sentence denotes The epitopes recognized by our panel of MC antibodies with NT activity may also be present on E2 peplomer protein.
TextSentencer_T106 14480-14581 Sentence denotes MC antibodies reacting in ELISA but having no NT activity may recognize epitopes on N or E1 proteins.
TextSentencer_T106 14480-14581 Sentence denotes MC antibodies reacting in ELISA but having no NT activity may recognize epitopes on N or E1 proteins.
TextSentencer_T106 14480-14581 Sentence denotes MC antibodies reacting in ELISA but having no NT activity may recognize epitopes on N or E1 proteins.
TextSentencer_T107 14582-14726 Sentence denotes Further studies are required to elucidate the epitopes for these MC antibodies by Western immunoblotting, immunoprecipitation and other methods.
TextSentencer_T107 14582-14726 Sentence denotes Further studies are required to elucidate the epitopes for these MC antibodies by Western immunoblotting, immunoprecipitation and other methods.
TextSentencer_T107 14582-14726 Sentence denotes Further studies are required to elucidate the epitopes for these MC antibodies by Western immunoblotting, immunoprecipitation and other methods.
TextSentencer_T108 14727-14930 Sentence denotes Comparison of seven TGE strains by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences (Table II) .
TextSentencer_T108 14727-14930 Sentence denotes Comparison of seven TGE strains by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences (Table II) .
TextSentencer_T108 14727-14930 Sentence denotes Comparison of seven TGE strains by NT tests using our panel of MC antibodies confirmed their close antigenic relationships, but also revealed the occurrence of distinct antigenic differences (Table II) .
TextSentencer_T109 14931-14985 Sentence denotes Similar results were reported by Laude et al. (1986) .
TextSentencer_T109 14931-14985 Sentence denotes Similar results were reported by Laude et al. (1986) .
TextSentencer_T109 14931-14985 Sentence denotes Similar results were reported by Laude et al. (1986) .
TextSentencer_T110 14986-15146 Sentence denotes The results of NT tests on the seven TGE strains (Table II) suggest that there may be at least six NT-mediating determinants on the virion of the TO-163 strain.
TextSentencer_T110 14986-15146 Sentence denotes The results of NT tests on the seven TGE strains (Table II) suggest that there may be at least six NT-mediating determinants on the virion of the TO-163 strain.
TextSentencer_T110 14986-15146 Sentence denotes The results of NT tests on the seven TGE strains (Table II) suggest that there may be at least six NT-mediating determinants on the virion of the TO-163 strain.
TextSentencer_T111 15147-15181 Sentence denotes These six epitopes are as follows:
TextSentencer_T111 15147-15181 Sentence denotes These six epitopes are as follows:
TextSentencer_T111 15147-15181 Sentence denotes These six epitopes are as follows:
TextSentencer_T112 15182-16056 Sentence denotes (1) the epitope recognized by MC antibodies, 4-4 and 55-2, which showed high NT activity for all the virus strains; (2) the epitope recognized by MC antibody, 38-A, which showed low NT activity for all the virus strains; (3) the epitope recognized by MC antibody 16-C, which showed NT activity for all the virus strains except the Purdue strain: (4) the epitope recognized by MC antibody 9-C, which showed NT activity for all the virus strains except the Ukiha strain; (5) the epitope recognized by MC antibodies, 66-A and 75-4, which showed high NT activity for the TO-163, h-5 and Kanagawa strains, very low NT activity for the Purdue strain and no NT activity for the Ukiha, Shizuoka and Miller strains; (6) the epitope recognized by MC antibody 39-7, which showed NT activity for the TO-163, h-5 and Kanagawa strains, but no NT activity for the other four virus strains.
TextSentencer_T112 15182-16056 Sentence denotes (1) the epitope recognized by MC antibodies, 4-4 and 55-2, which showed high NT activity for all the virus strains; (2) the epitope recognized by MC antibody, 38-A, which showed low NT activity for all the virus strains; (3) the epitope recognized by MC antibody 16-C, which showed NT activity for all the virus strains except the Purdue strain: (4) the epitope recognized by MC antibody 9-C, which showed NT activity for all the virus strains except the Ukiha strain; (5) the epitope recognized by MC antibodies, 66-A and 75-4, which showed high NT activity for the TO-163, h-5 and Kanagawa strains, very low NT activity for the Purdue strain and no NT activity for the Ukiha, Shizuoka and Miller strains; (6) the epitope recognized by MC antibody 39-7, which showed NT activity for the TO-163, h-5 and Kanagawa strains, but no NT activity for the other four virus strains.
TextSentencer_T112 15182-16056 Sentence denotes (1) the epitope recognized by MC antibodies, 4-4 and 55-2, which showed high NT activity for all the virus strains; (2) the epitope recognized by MC antibody, 38-A, which showed low NT activity for all the virus strains; (3) the epitope recognized by MC antibody 16-C, which showed NT activity for all the virus strains except the Purdue strain: (4) the epitope recognized by MC antibody 9-C, which showed NT activity for all the virus strains except the Ukiha strain; (5) the epitope recognized by MC antibodies, 66-A and 75-4, which showed high NT activity for the TO-163, h-5 and Kanagawa strains, very low NT activity for the Purdue strain and no NT activity for the Ukiha, Shizuoka and Miller strains; (6) the epitope recognized by MC antibody 39-7, which showed NT activity for the TO-163, h-5 and Kanagawa strains, but no NT activity for the other four virus strains.
TextSentencer_T113 16057-16313 Sentence denotes The presence of six epitopes was confirmed by the competitive binding assay (Table IV) : MC antibodies 4-4 and 55-2 recognized one epitope and 66-A and 75-4 recognized another, while antibodies 38-A, 16-C, 9-C and 39-7 each recognized one distinct epitope.
TextSentencer_T113 16057-16313 Sentence denotes The presence of six epitopes was confirmed by the competitive binding assay (Table IV) : MC antibodies 4-4 and 55-2 recognized one epitope and 66-A and 75-4 recognized another, while antibodies 38-A, 16-C, 9-C and 39-7 each recognized one distinct epitope.
TextSentencer_T113 16057-16313 Sentence denotes The presence of six epitopes was confirmed by the competitive binding assay (Table IV) : MC antibodies 4-4 and 55-2 recognized one epitope and 66-A and 75-4 recognized another, while antibodies 38-A, 16-C, 9-C and 39-7 each recognized one distinct epitope.
TextSentencer_T114 16314-16385 Sentence denotes These results are in good agreement with those of NT tests (Table II) .
TextSentencer_T114 16314-16385 Sentence denotes These results are in good agreement with those of NT tests (Table II) .
TextSentencer_T114 16314-16385 Sentence denotes These results are in good agreement with those of NT tests (Table II) .
TextSentencer_T115 16386-16534 Sentence denotes MC antibodies 66-A and 75-4 blocked enzyme-labeled antibodies 4-4, 55-2, 38-A, 16-C, 9-C and 39-7 to some extent but the reason for this is obscure.
TextSentencer_T115 16386-16534 Sentence denotes MC antibodies 66-A and 75-4 blocked enzyme-labeled antibodies 4-4, 55-2, 38-A, 16-C, 9-C and 39-7 to some extent but the reason for this is obscure.
TextSentencer_T115 16386-16534 Sentence denotes MC antibodies 66-A and 75-4 blocked enzyme-labeled antibodies 4-4, 55-2, 38-A, 16-C, 9-C and 39-7 to some extent but the reason for this is obscure.
TextSentencer_T116 16535-16660 Sentence denotes As shown in Table II , the seven TGE strains were similarly neutralized with polyclonal antibodies against the TO-163 strain.
TextSentencer_T116 16535-16660 Sentence denotes As shown in Table II , the seven TGE strains were similarly neutralized with polyclonal antibodies against the TO-163 strain.
TextSentencer_T116 16535-16660 Sentence denotes As shown in Table II , the seven TGE strains were similarly neutralized with polyclonal antibodies against the TO-163 strain.
TextSentencer_T117 16661-16799 Sentence denotes This finding suggests that subunit vaccines containing E2 protein of the TO-163 strain may have protective efficacy against other strains.
TextSentencer_T117 16661-16799 Sentence denotes This finding suggests that subunit vaccines containing E2 protein of the TO-163 strain may have protective efficacy against other strains.
TextSentencer_T117 16661-16799 Sentence denotes This finding suggests that subunit vaccines containing E2 protein of the TO-163 strain may have protective efficacy against other strains.
TextSentencer_T118 16800-16872 Sentence denotes MC antibodies 4-4 and 55-2 may be useful for purification of E2 protein.
TextSentencer_T118 16800-16872 Sentence denotes MC antibodies 4-4 and 55-2 may be useful for purification of E2 protein.
TextSentencer_T118 16800-16872 Sentence denotes MC antibodies 4-4 and 55-2 may be useful for purification of E2 protein.