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At 2 dpi, mouse lungs were harvested. (B) Viral loads were tittered and plotted. (C) At 4 dpi, lungs were harvested and cytokine production in mRNA level was measured by real-time qPCR. (D) At 4 dpi, lung fluid was harvested and cytokine production was measured by ELISA essay. (E-G) The 6-to 8-week-old CARMA3 WT and KO mice (n R 5 per group) were intranasally inoculated with 10 7 PFUs of VSV-GFP per mouse. (E) At 2 dpi, olfactory bulbs (OBs) were harvested and imaged. (F) Viral loads in OBs were tittered and plotted in log scale. (G) At 1 dpi, OBs were harvested and cytokine production in mRNA level was measured by qPCR. |
