PMC:3265313 / 15452-15454 JSONTXT
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The Effects of Temperature and Relative Humidity on the Viability of the SARS Coronavirus
Abstract
The main route of transmission of SARS CoV infection is presumed to be respiratory droplets. However the virus is also detectable in other body fluids and excreta. The stability of the virus at different temperatures and relative humidity on smooth surfaces were studied. The dried virus on smooth surfaces retained its viability for over 5 days at temperatures of 22–25°C and relative humidity of 40–50%, that is, typical air-conditioned environments. However, virus viability was rapidly lost (>3 log10) at higher temperatures and higher relative humidity (e.g., 38°C, and relative humidity of >95%). The better stability of SARS coronavirus at low temperature and low humidity environment may facilitate its transmission in community in subtropical area (such as Hong Kong) during the spring and in air-conditioned environments. It may also explain why some Asian countries in tropical area (such as Malaysia, Indonesia or Thailand) with high temperature and high relative humidity environment did not have major community outbreaks of SARS.
1. Introduction
Severe acute respiratory syndrome (SARS), was a new emerging disease associated with severe pneumonia and spread to involve over 30 countries in 5 continents in 2003. A novel coronavirus was identified as its cause [1–3]. SARS had a dramatic impact on health care services and economies of affected countries, and the overall mortality rate was estimated to be 9%, but rising to 50% in those aged 60 or above [4]. A notable feature of this disease was its predilection for transmission in the health care setting and to close family and social contacts. The disease is presumed to be spread by droplets, close direct or indirect contact, but the relative importance of these routes of transmission is presently unclear. A study showed that viral aerosol generation by a patient with SARS was possible and therefore airborne droplet transmission was a possible means of transmission [5]. However, the role of fomites and environmental contamination in transmission of infection is presently still unclear. An outbreak of disease affecting over 300 residents in high-rise apartment block (Amoy Gardens) in Hong Kong could not be explained by respiratory droplet transmission from infected patients [6]. Infectious virus is detectable in the faeces [7], and aerosolization of virus in contaminated faeces is believed to be the mode of transmission of this outbreak [8].
We and others have reported that infectivity of SARS CoV (SARS coronavirus) was lost after heating at 56°C for 15 minutes but that it was stable for at least 2 days following drying on plastic. It was completely inactivated by common fixatives used in laboratory [9, 10]. Another study showed that it was inactivated by ultraviolet light, alkaline (pH > 12), or acidic (pH < 3) conditions [11]. Human coronaviruses have been shown to survive in PBS or culture medium with 5–10% FCS for several days [12–14] but they only survive a few hours after drying [13, 14]. There have been some studies reporting an association between the SARS outbreak, metrological factors, and air pollution [15–17]. Thus, information on the survival of the SARS coronavirus (SCoV) in the environment at different temperature and humidity conditions is of significant interest to understanding virus transmission. A recent study using surrogate coronaviruses (transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHC)) has investigated the effect of air temperature and relative humidity on coronavirus survival on surface [18]. The survival effects of these environmental factors on SARS coronavirus remain unclear. In the present study, we report the stability of the SARS coronavirus at different temperatures and relative humidity.
2. Material and Methods
2.1. Virus Strain and Cell Line
The SARS CoV strain used in this study is HKU39849. Foetal monkey kidney cells (FRhK-4) were cultured in minimal essential medium (MEM, Gibco, USA) with 10% foetal calf serum and penicillin streptomycin (Gibco, USA) at 37°C in 5% CO2 and were used for growing stock virus and for titration of viral infectivity [1, 2].
2.2. Preparation of Stock Virus
Stock virus was harvested when infection approximately 75% of the cell monolayer of a virus infected flask manifested cytopathic effect (CPE). Infected cells were subjected to one cycle of freeze and thaw centrifuged at 2000 rpm for 20 minutes to remove cell debris and the culture supernatant was aliquoted and stored at −80°C until use.
2.3. Determination of Tissue Culture Infectious Dose (50%) (TCID50)
96-well microtitre plates containing 100 μL of confluent FRhK-4 were infected with 100 μL of serial 10-fold of dilutions of stock virus in minimal essential medium with 1% FCS (maintenance medium) starting from 10−1 to 108. Titrations were done in quadruplicate. Infected cells were incubated for 4 days at 37°C. Appearance of CPE was recorded daily. TCID50 was determined according to Reed and the Muench method [19].
2.4. Effect of Drying, Heat, and Relative Humidity
Ten microlitre of maintenance medium containing 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying.
The experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated.
2.5. Infectivity Assay
The infectivity of residual virus was titrated in quadruplicate on 96-well microtitre plates containing 100 μL of confluent FRhK-4 cells. 100 μL of serial 10-fold of dilutions of virus in maintenance medium starting from 10−1 to 108 was added into FRhK-4 cells. The infected cells were incubated at 37°C for 4 days. Appearance of CPE was recorded daily. TCID50 was determined according to the Reed and Muench method [19].
3. Results
Ten microlitre of 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plate (representing a nonporous surface) and dried. The dried virus was then incubated at different temperatures (38°C, 33°C, 28°C) at different relative humidity (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr and the residual viral infectivity was titrated. A similar experiment was conducted at room temperature and relative humidity of about 40–50% (air-conditioned room) for up to 4 weeks. Virus dried on plastic retained viability for up to 5 days at 22~25°C at relative humidity of 40~50% with only 1 log10 loss of titre (Figure 1). After that virus infectivity is gradually lost ever time. Loss of virus infectivity in solution was generally similar to dried virus under these environmental conditions. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites, especially in air-conditioned environments.
High relative humidity (>95%) at comparatively low temperature (28°C and 33°C) did not affect the virus infectivity significantly (Figure 2(a)). High temperature (38°C) at 80–90% relative humidity led to a 0.25~2 log10 loss of titre at 24 hr (Figure 2(b)). However, if the dried virus was stored at high temperature (38°C) and high relative humidity (>95%), there was a further ~1.5 log loss of titre for each time point up to 24 hr (0.38~3.38 log10) when compared with high temperature (38°C) at a lower relative humidity 80–90% (Figures 3(a)–3(c)).
4. Discussion
Viruses do not replicate outside living cell but infectious virus may persist on contaminated environmental surfaces and the duration of persistence of viable virus is affected markedly by temperature and humidity. Contaminated surfaces are known to be significant vectors in the transmission of infections in the hospital setting as well as the community. The role of fomites in the transmission of RSV has been clearly demonstrated [20]. Survival of viruses on a variety of fomites has been studied for influenza viruses, paramyxoviruses, poxviruses, and retroviruses [21]. The human coronavirus associated with the common cold was reported to remain viable only for 3 hours on environmental surfaces after drying, although it remains viable for many days in liquid suspension [13]. Parainfluenza and RSV viruses were viable after drying on surfaces for 2 and 6 hours, respectively [20, 22]. In aerosolised form, human coronavirus 229E is generally less stable in high humidity [12]. The environmental stability of SCoV was previously unknown and this information is clearly important for understanding the mechanisms of transmission of this virus in a hospital and community setting.
In the present study, we have demonstrated that SARS CoV can survive at least two weeks after drying at temperature and humidity conditions found in an air-conditioned environment. The virus is stable for 3 weeks at room temperature in a liquid environment but it is easily killed by heat at 56°C for 15 minutes [9]. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites. These results may indicate that contaminated surfaces may play a major role in transmission of infection in the hospital and the community.
Our studies indicate that SCoV is relatively more stable than the human coronaviruses 229E or OC43 and some other viral respiratory pathogens such as respiratory syncytial virus. These findings suggest that, while direct droplet transmission is an important route of transmission [23], the role of fomites and environmental contamination in virus transmission may play a significant role in virus transmission. In particular, fomites may contribute to the continued transmission of infection in the nosocomial setting that continues to occur in spite of the great attention and stringent precautions taken to prevent droplet spread. In addition to droplet precautions, reenforcing contact precautions and hand washing is called for.
Faecal contamination of SCoV coronavirus may thus be an effective route of transmission of the disease. The outbreak in Amoy Garden in Hong Kong which affected over 300 residents in a single-apartment block with thought to have been transmitted by contaminated sewage. The stability of the virus on environmental surfaces and its presence in faeces indicates the potential that fecal contamination of fresh-food production may pose a threat for virus transmission; especially in countries with poor sanitation and sewage disposal systems and that studies to address this possibility are needed.
In this study, we showed that high temperature at high relative humidity has a synergistic effect on inactivation of SARS CoV viability while lower temperatures and low humidity support prolonged survival of virus on contaminated surfaces. The environmental conditions of countries such as Malaysia, Indonesia, and Thailand are thus not conducive to the prolonged survival of the virus. In countries such as Singapore and Hong Kong where there is a intensive use of air-conditioning, transmission largely occurred in well-air-conditioned environments such as hospitals or hotels. Further, a separate study has shown that during the epidemic, the risk of increased daily incidence of SARS was 18.18-fold higher in days with a lower air temperature than in days with a higher temperature in Hong Kong [24] and other regions [15–17]. Taken together, these observations may explain why some Asian countries in tropical area (with high temperature at high relative humidity) such as Malaysia, Indonesia, and Thailand did not have nosocomial outbreaks of SARS (Tables 1 and 2(a)–2(c)). It may also explain why Singapore, which is also in tropical area (Table 2(d)), had most of its SARS outbreaks in hospitals (air-conditioned environment). Interestingly, during the outbreak of SARS in Guangzhou, clinicians kept the windows of patient rooms open and well ventilated and these may well have reduced virus survival and this reduced nosocomial transmission. SARS CoV can retain its infectivity up to 2 weeks at low temperature and low humidity environment, which might facilitate the virus transmission in community as in Hong Kong which locates in subtropical area (Table 2(e)). Other environmental factors including wind velocity, daily sunlight, and air pressure, had shown to be associated with SARS epidemic, should also be considered [16, 17]. The dynamics of SARS epidemic involves multiple factors including physical property of virus, outdoor and indoor environments, hygiene, space, and genetic predispositions [10, 15–17, 24, 25]. Understanding the stability of viruses in different temperature and humidity conditions is important in understanding transmission of novel infectious agent including that of the recent influenza Apandemic H1N12009.
Conflict of Interests
The authors declare that there is no conflict of interest.
Figure 1 Residual virus infectivity at 22–25°C with relative humidity 40–50% (starting titre 105/10 μL) and at 33°C or 38°C with relative humidity >95%.
Figure 2 Infectivity of SARS Coronavirus (105/10 μL) to different temperatures at (a) >95% relative humidity, (b) >80–89%.
Figure 3 Infectivity of SARS Coronavirus (starting titre 105/10 μL) at different relative humidity at (a) 38°C, (b) 33°C, and (c) 28°C.
Table 1 WHO SARS report—based on data as of the 31st December 2003.
Areas Total Medan age Deaths Case fatality Ratio (%) No. of imported Cases (%) No. of HCW (%) First case Last case
China 5327 NKn 349 7 NA 1002 (19) Nov-02 Jun-03
Hong Kong 1755 40 299 17 NA 386 (22) Feb-03 May-03
Taiwan 346 42 37 11 21 (6) 68 (20) Feb-03 Jun-03
Singapore 238 35 33 14 8 (3) 97 (41) Feb-03 May-03
Viet Nam 63 43 5 8 1 (2) 36 (57) Feb-03 Apr-03
Indonesia 2 56 0 0 2 (100) 0 (0) Apr-03 Apr-03
Malaysia 5 30 2 40 5 (100) 0 (0) Mar-03 Apr-03
Thailand 9 42 2 22 9 (100) 1 (11) Mar-03 May-03
Philippines 14 41 2 14 7 (50) 4 (29) Feb-03 May-03
Total 8096 774 9.6 142 1706 (21)
Table 2 A summary of the meteorological data of 2005 in average weather conditions*.
Month Average sunlight (hours) Temperature Discomfort from heat and humidity Relative humidity
Min Max am pm
(a) Kuala Lumpur, Malaysia
Jan 6 22 32 High 97 60
Feb 7 22 33 High 97 60
March 7 23 33 High 97 58
April 6 23 33 High 97 63
May 6 23 33 High 97 66
June 7 22 33 High 96 63
July 7 23 32 High 95 63
Aug 6 23 32 High 96 62
Sept 6 23 32 High 96 64
Oct 5 23 32 High 96 65
Nov 5 23 32 High 97 66
Dec 5 22 32 High 97 61
(b) Jakarta, Indonesia
Jan 5 23 29 High 95 75
Feb 5 23 29 High 95 75
March 6 23 30 High 94 73
April 7 24 31 High 94 71
May 7 24 31 High 94 69
June 7 23 31 High 93 67
July 7 23 31 High 92 64
Aug 8 23 31 High 90 61
Sept 8 23 31 High 90 62
Oct 7 23 31 High 90 64
Nov 6 23 30 High 92 68
Dec 5 23 29 High 92 71
(c) Bangkok, Thailand
Jan 9 20 32 High 91 53
Feb 8 22 33 High 92 55
March 9 24 34 High 92 56
April 8 25 35 Extreme 90 58
May 8 25 34 Extreme 91 64
June 6 24 33 Extreme 90 67
July 5 24 32 High 91 66
Aug 5 24 32 High 92 66
Sept 5 24 32 High 94 70
Oct 6 24 31 High 93 70
Nov 8 22 31 High 92 65
Dec 9 20 31 High 91 56
(d) Singapore
Jan 5 23 30 High 82 78
Feb 7 23 31 High 77 71
March 6 24 31 High 76 70
April 6 24 31 High 77 74
May 6 24 32 Extreme 79 73
June 6 24 31 High 79 73
July 6 24 31 High 79 72
Aug 6 24 31 High 78 72
Sept 5 24 31 High 79 72
Oct 5 23 31 High 78 72
Nov 5 23 31 High 79 75
Dec 4 23 31 High 82 78
(e) Hong Kong
Jan 5 13 18 — 77 66
Feb 4 13 17 — 82 73
March 3 16 19 — 84 74
April 4 19 24 Medium 87 77
May 5 23 28 Medium 87 78
June 5 26 29 High 86 77
July 8 26 31 High 87 77
Aug 6 26 31 High 87 77
Sept 6 25 29 High 83 72
Oct 7 23 27 Medium 75 63
Nov 7 18 23 Moderate 73 60
Dec 6 15 20 — 74 63
*Data is available at BBC weather website (http://www.bbc.co.uk/weather/world/city_guides/results).
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| article-title | The Effects of Temperature and Relative Humidity on the Viability of the SARS Coronavirus |
| abstract | The main route of transmission of SARS CoV infection is presumed to be respiratory droplets. However the virus is also detectable in other body fluids and excreta. The stability of the virus at different temperatures and relative humidity on smooth surfaces were studied. The dried virus on smooth surfaces retained its viability for over 5 days at temperatures of 22–25°C and relative humidity of 40–50%, that is, typical air-conditioned environments. However, virus viability was rapidly lost (>3 log10) at higher temperatures and higher relative humidity (e.g., 38°C, and relative humidity of >95%). The better stability of SARS coronavirus at low temperature and low humidity environment may facilitate its transmission in community in subtropical area (such as Hong Kong) during the spring and in air-conditioned environments. It may also explain why some Asian countries in tropical area (such as Malaysia, Indonesia or Thailand) with high temperature and high relative humidity environment did not have major community outbreaks of SARS. |
| p | The main route of transmission of SARS CoV infection is presumed to be respiratory droplets. However the virus is also detectable in other body fluids and excreta. The stability of the virus at different temperatures and relative humidity on smooth surfaces were studied. The dried virus on smooth surfaces retained its viability for over 5 days at temperatures of 22–25°C and relative humidity of 40–50%, that is, typical air-conditioned environments. However, virus viability was rapidly lost (>3 log10) at higher temperatures and higher relative humidity (e.g., 38°C, and relative humidity of >95%). The better stability of SARS coronavirus at low temperature and low humidity environment may facilitate its transmission in community in subtropical area (such as Hong Kong) during the spring and in air-conditioned environments. It may also explain why some Asian countries in tropical area (such as Malaysia, Indonesia or Thailand) with high temperature and high relative humidity environment did not have major community outbreaks of SARS. |
| body | 1. Introduction Severe acute respiratory syndrome (SARS), was a new emerging disease associated with severe pneumonia and spread to involve over 30 countries in 5 continents in 2003. A novel coronavirus was identified as its cause [1–3]. SARS had a dramatic impact on health care services and economies of affected countries, and the overall mortality rate was estimated to be 9%, but rising to 50% in those aged 60 or above [4]. A notable feature of this disease was its predilection for transmission in the health care setting and to close family and social contacts. The disease is presumed to be spread by droplets, close direct or indirect contact, but the relative importance of these routes of transmission is presently unclear. A study showed that viral aerosol generation by a patient with SARS was possible and therefore airborne droplet transmission was a possible means of transmission [5]. However, the role of fomites and environmental contamination in transmission of infection is presently still unclear. An outbreak of disease affecting over 300 residents in high-rise apartment block (Amoy Gardens) in Hong Kong could not be explained by respiratory droplet transmission from infected patients [6]. Infectious virus is detectable in the faeces [7], and aerosolization of virus in contaminated faeces is believed to be the mode of transmission of this outbreak [8]. We and others have reported that infectivity of SARS CoV (SARS coronavirus) was lost after heating at 56°C for 15 minutes but that it was stable for at least 2 days following drying on plastic. It was completely inactivated by common fixatives used in laboratory [9, 10]. Another study showed that it was inactivated by ultraviolet light, alkaline (pH > 12), or acidic (pH < 3) conditions [11]. Human coronaviruses have been shown to survive in PBS or culture medium with 5–10% FCS for several days [12–14] but they only survive a few hours after drying [13, 14]. There have been some studies reporting an association between the SARS outbreak, metrological factors, and air pollution [15–17]. Thus, information on the survival of the SARS coronavirus (SCoV) in the environment at different temperature and humidity conditions is of significant interest to understanding virus transmission. A recent study using surrogate coronaviruses (transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHC)) has investigated the effect of air temperature and relative humidity on coronavirus survival on surface [18]. The survival effects of these environmental factors on SARS coronavirus remain unclear. In the present study, we report the stability of the SARS coronavirus at different temperatures and relative humidity. 2. Material and Methods 2.1. Virus Strain and Cell Line The SARS CoV strain used in this study is HKU39849. Foetal monkey kidney cells (FRhK-4) were cultured in minimal essential medium (MEM, Gibco, USA) with 10% foetal calf serum and penicillin streptomycin (Gibco, USA) at 37°C in 5% CO2 and were used for growing stock virus and for titration of viral infectivity [1, 2]. 2.2. Preparation of Stock Virus Stock virus was harvested when infection approximately 75% of the cell monolayer of a virus infected flask manifested cytopathic effect (CPE). Infected cells were subjected to one cycle of freeze and thaw centrifuged at 2000 rpm for 20 minutes to remove cell debris and the culture supernatant was aliquoted and stored at −80°C until use. 2.3. Determination of Tissue Culture Infectious Dose (50%) (TCID50) 96-well microtitre plates containing 100 μL of confluent FRhK-4 were infected with 100 μL of serial 10-fold of dilutions of stock virus in minimal essential medium with 1% FCS (maintenance medium) starting from 10−1 to 108. Titrations were done in quadruplicate. Infected cells were incubated for 4 days at 37°C. Appearance of CPE was recorded daily. TCID50 was determined according to Reed and the Muench method [19]. 2.4. Effect of Drying, Heat, and Relative Humidity Ten microlitre of maintenance medium containing 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying. The experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated. 2.5. Infectivity Assay The infectivity of residual virus was titrated in quadruplicate on 96-well microtitre plates containing 100 μL of confluent FRhK-4 cells. 100 μL of serial 10-fold of dilutions of virus in maintenance medium starting from 10−1 to 108 was added into FRhK-4 cells. The infected cells were incubated at 37°C for 4 days. Appearance of CPE was recorded daily. TCID50 was determined according to the Reed and Muench method [19]. 3. Results Ten microlitre of 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plate (representing a nonporous surface) and dried. The dried virus was then incubated at different temperatures (38°C, 33°C, 28°C) at different relative humidity (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr and the residual viral infectivity was titrated. A similar experiment was conducted at room temperature and relative humidity of about 40–50% (air-conditioned room) for up to 4 weeks. Virus dried on plastic retained viability for up to 5 days at 22~25°C at relative humidity of 40~50% with only 1 log10 loss of titre (Figure 1). After that virus infectivity is gradually lost ever time. Loss of virus infectivity in solution was generally similar to dried virus under these environmental conditions. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites, especially in air-conditioned environments. High relative humidity (>95%) at comparatively low temperature (28°C and 33°C) did not affect the virus infectivity significantly (Figure 2(a)). High temperature (38°C) at 80–90% relative humidity led to a 0.25~2 log10 loss of titre at 24 hr (Figure 2(b)). However, if the dried virus was stored at high temperature (38°C) and high relative humidity (>95%), there was a further ~1.5 log loss of titre for each time point up to 24 hr (0.38~3.38 log10) when compared with high temperature (38°C) at a lower relative humidity 80–90% (Figures 3(a)–3(c)). 4. Discussion Viruses do not replicate outside living cell but infectious virus may persist on contaminated environmental surfaces and the duration of persistence of viable virus is affected markedly by temperature and humidity. Contaminated surfaces are known to be significant vectors in the transmission of infections in the hospital setting as well as the community. The role of fomites in the transmission of RSV has been clearly demonstrated [20]. Survival of viruses on a variety of fomites has been studied for influenza viruses, paramyxoviruses, poxviruses, and retroviruses [21]. The human coronavirus associated with the common cold was reported to remain viable only for 3 hours on environmental surfaces after drying, although it remains viable for many days in liquid suspension [13]. Parainfluenza and RSV viruses were viable after drying on surfaces for 2 and 6 hours, respectively [20, 22]. In aerosolised form, human coronavirus 229E is generally less stable in high humidity [12]. The environmental stability of SCoV was previously unknown and this information is clearly important for understanding the mechanisms of transmission of this virus in a hospital and community setting. In the present study, we have demonstrated that SARS CoV can survive at least two weeks after drying at temperature and humidity conditions found in an air-conditioned environment. The virus is stable for 3 weeks at room temperature in a liquid environment but it is easily killed by heat at 56°C for 15 minutes [9]. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites. These results may indicate that contaminated surfaces may play a major role in transmission of infection in the hospital and the community. Our studies indicate that SCoV is relatively more stable than the human coronaviruses 229E or OC43 and some other viral respiratory pathogens such as respiratory syncytial virus. These findings suggest that, while direct droplet transmission is an important route of transmission [23], the role of fomites and environmental contamination in virus transmission may play a significant role in virus transmission. In particular, fomites may contribute to the continued transmission of infection in the nosocomial setting that continues to occur in spite of the great attention and stringent precautions taken to prevent droplet spread. In addition to droplet precautions, reenforcing contact precautions and hand washing is called for. Faecal contamination of SCoV coronavirus may thus be an effective route of transmission of the disease. The outbreak in Amoy Garden in Hong Kong which affected over 300 residents in a single-apartment block with thought to have been transmitted by contaminated sewage. The stability of the virus on environmental surfaces and its presence in faeces indicates the potential that fecal contamination of fresh-food production may pose a threat for virus transmission; especially in countries with poor sanitation and sewage disposal systems and that studies to address this possibility are needed. In this study, we showed that high temperature at high relative humidity has a synergistic effect on inactivation of SARS CoV viability while lower temperatures and low humidity support prolonged survival of virus on contaminated surfaces. The environmental conditions of countries such as Malaysia, Indonesia, and Thailand are thus not conducive to the prolonged survival of the virus. In countries such as Singapore and Hong Kong where there is a intensive use of air-conditioning, transmission largely occurred in well-air-conditioned environments such as hospitals or hotels. Further, a separate study has shown that during the epidemic, the risk of increased daily incidence of SARS was 18.18-fold higher in days with a lower air temperature than in days with a higher temperature in Hong Kong [24] and other regions [15–17]. Taken together, these observations may explain why some Asian countries in tropical area (with high temperature at high relative humidity) such as Malaysia, Indonesia, and Thailand did not have nosocomial outbreaks of SARS (Tables 1 and 2(a)–2(c)). It may also explain why Singapore, which is also in tropical area (Table 2(d)), had most of its SARS outbreaks in hospitals (air-conditioned environment). Interestingly, during the outbreak of SARS in Guangzhou, clinicians kept the windows of patient rooms open and well ventilated and these may well have reduced virus survival and this reduced nosocomial transmission. SARS CoV can retain its infectivity up to 2 weeks at low temperature and low humidity environment, which might facilitate the virus transmission in community as in Hong Kong which locates in subtropical area (Table 2(e)). Other environmental factors including wind velocity, daily sunlight, and air pressure, had shown to be associated with SARS epidemic, should also be considered [16, 17]. The dynamics of SARS epidemic involves multiple factors including physical property of virus, outdoor and indoor environments, hygiene, space, and genetic predispositions [10, 15–17, 24, 25]. Understanding the stability of viruses in different temperature and humidity conditions is important in understanding transmission of novel infectious agent including that of the recent influenza Apandemic H1N12009. |
| sec | 1. Introduction Severe acute respiratory syndrome (SARS), was a new emerging disease associated with severe pneumonia and spread to involve over 30 countries in 5 continents in 2003. A novel coronavirus was identified as its cause [1–3]. SARS had a dramatic impact on health care services and economies of affected countries, and the overall mortality rate was estimated to be 9%, but rising to 50% in those aged 60 or above [4]. A notable feature of this disease was its predilection for transmission in the health care setting and to close family and social contacts. The disease is presumed to be spread by droplets, close direct or indirect contact, but the relative importance of these routes of transmission is presently unclear. A study showed that viral aerosol generation by a patient with SARS was possible and therefore airborne droplet transmission was a possible means of transmission [5]. However, the role of fomites and environmental contamination in transmission of infection is presently still unclear. An outbreak of disease affecting over 300 residents in high-rise apartment block (Amoy Gardens) in Hong Kong could not be explained by respiratory droplet transmission from infected patients [6]. Infectious virus is detectable in the faeces [7], and aerosolization of virus in contaminated faeces is believed to be the mode of transmission of this outbreak [8]. We and others have reported that infectivity of SARS CoV (SARS coronavirus) was lost after heating at 56°C for 15 minutes but that it was stable for at least 2 days following drying on plastic. It was completely inactivated by common fixatives used in laboratory [9, 10]. Another study showed that it was inactivated by ultraviolet light, alkaline (pH > 12), or acidic (pH < 3) conditions [11]. Human coronaviruses have been shown to survive in PBS or culture medium with 5–10% FCS for several days [12–14] but they only survive a few hours after drying [13, 14]. There have been some studies reporting an association between the SARS outbreak, metrological factors, and air pollution [15–17]. Thus, information on the survival of the SARS coronavirus (SCoV) in the environment at different temperature and humidity conditions is of significant interest to understanding virus transmission. A recent study using surrogate coronaviruses (transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHC)) has investigated the effect of air temperature and relative humidity on coronavirus survival on surface [18]. The survival effects of these environmental factors on SARS coronavirus remain unclear. In the present study, we report the stability of the SARS coronavirus at different temperatures and relative humidity. |
| title | 1. Introduction |
| p | Severe acute respiratory syndrome (SARS), was a new emerging disease associated with severe pneumonia and spread to involve over 30 countries in 5 continents in 2003. A novel coronavirus was identified as its cause [1–3]. SARS had a dramatic impact on health care services and economies of affected countries, and the overall mortality rate was estimated to be 9%, but rising to 50% in those aged 60 or above [4]. A notable feature of this disease was its predilection for transmission in the health care setting and to close family and social contacts. The disease is presumed to be spread by droplets, close direct or indirect contact, but the relative importance of these routes of transmission is presently unclear. A study showed that viral aerosol generation by a patient with SARS was possible and therefore airborne droplet transmission was a possible means of transmission [5]. However, the role of fomites and environmental contamination in transmission of infection is presently still unclear. An outbreak of disease affecting over 300 residents in high-rise apartment block (Amoy Gardens) in Hong Kong could not be explained by respiratory droplet transmission from infected patients [6]. Infectious virus is detectable in the faeces [7], and aerosolization of virus in contaminated faeces is believed to be the mode of transmission of this outbreak [8]. |
| p | We and others have reported that infectivity of SARS CoV (SARS coronavirus) was lost after heating at 56°C for 15 minutes but that it was stable for at least 2 days following drying on plastic. It was completely inactivated by common fixatives used in laboratory [9, 10]. Another study showed that it was inactivated by ultraviolet light, alkaline (pH > 12), or acidic (pH < 3) conditions [11]. Human coronaviruses have been shown to survive in PBS or culture medium with 5–10% FCS for several days [12–14] but they only survive a few hours after drying [13, 14]. There have been some studies reporting an association between the SARS outbreak, metrological factors, and air pollution [15–17]. Thus, information on the survival of the SARS coronavirus (SCoV) in the environment at different temperature and humidity conditions is of significant interest to understanding virus transmission. A recent study using surrogate coronaviruses (transmissible gastroenteritis virus (TGEV) and mouse hepatitis virus (MHC)) has investigated the effect of air temperature and relative humidity on coronavirus survival on surface [18]. The survival effects of these environmental factors on SARS coronavirus remain unclear. In the present study, we report the stability of the SARS coronavirus at different temperatures and relative humidity. |
| sec | 2. Material and Methods 2.1. Virus Strain and Cell Line The SARS CoV strain used in this study is HKU39849. Foetal monkey kidney cells (FRhK-4) were cultured in minimal essential medium (MEM, Gibco, USA) with 10% foetal calf serum and penicillin streptomycin (Gibco, USA) at 37°C in 5% CO2 and were used for growing stock virus and for titration of viral infectivity [1, 2]. 2.2. Preparation of Stock Virus Stock virus was harvested when infection approximately 75% of the cell monolayer of a virus infected flask manifested cytopathic effect (CPE). Infected cells were subjected to one cycle of freeze and thaw centrifuged at 2000 rpm for 20 minutes to remove cell debris and the culture supernatant was aliquoted and stored at −80°C until use. 2.3. Determination of Tissue Culture Infectious Dose (50%) (TCID50) 96-well microtitre plates containing 100 μL of confluent FRhK-4 were infected with 100 μL of serial 10-fold of dilutions of stock virus in minimal essential medium with 1% FCS (maintenance medium) starting from 10−1 to 108. Titrations were done in quadruplicate. Infected cells were incubated for 4 days at 37°C. Appearance of CPE was recorded daily. TCID50 was determined according to Reed and the Muench method [19]. 2.4. Effect of Drying, Heat, and Relative Humidity Ten microlitre of maintenance medium containing 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying. The experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated. 2.5. Infectivity Assay The infectivity of residual virus was titrated in quadruplicate on 96-well microtitre plates containing 100 μL of confluent FRhK-4 cells. 100 μL of serial 10-fold of dilutions of virus in maintenance medium starting from 10−1 to 108 was added into FRhK-4 cells. The infected cells were incubated at 37°C for 4 days. Appearance of CPE was recorded daily. TCID50 was determined according to the Reed and Muench method [19]. |
| title | 2. Material and Methods |
| sec | 2.1. Virus Strain and Cell Line The SARS CoV strain used in this study is HKU39849. Foetal monkey kidney cells (FRhK-4) were cultured in minimal essential medium (MEM, Gibco, USA) with 10% foetal calf serum and penicillin streptomycin (Gibco, USA) at 37°C in 5% CO2 and were used for growing stock virus and for titration of viral infectivity [1, 2]. |
| title | 2.1. Virus Strain and Cell Line |
| p | The SARS CoV strain used in this study is HKU39849. Foetal monkey kidney cells (FRhK-4) were cultured in minimal essential medium (MEM, Gibco, USA) with 10% foetal calf serum and penicillin streptomycin (Gibco, USA) at 37°C in 5% CO2 and were used for growing stock virus and for titration of viral infectivity [1, 2]. |
| sec | 2.2. Preparation of Stock Virus Stock virus was harvested when infection approximately 75% of the cell monolayer of a virus infected flask manifested cytopathic effect (CPE). Infected cells were subjected to one cycle of freeze and thaw centrifuged at 2000 rpm for 20 minutes to remove cell debris and the culture supernatant was aliquoted and stored at −80°C until use. |
| title | 2.2. Preparation of Stock Virus |
| p | Stock virus was harvested when infection approximately 75% of the cell monolayer of a virus infected flask manifested cytopathic effect (CPE). Infected cells were subjected to one cycle of freeze and thaw centrifuged at 2000 rpm for 20 minutes to remove cell debris and the culture supernatant was aliquoted and stored at −80°C until use. |
| sec | 2.3. Determination of Tissue Culture Infectious Dose (50%) (TCID50) 96-well microtitre plates containing 100 μL of confluent FRhK-4 were infected with 100 μL of serial 10-fold of dilutions of stock virus in minimal essential medium with 1% FCS (maintenance medium) starting from 10−1 to 108. Titrations were done in quadruplicate. Infected cells were incubated for 4 days at 37°C. Appearance of CPE was recorded daily. TCID50 was determined according to Reed and the Muench method [19]. |
| title | 2.3. Determination of Tissue Culture Infectious Dose (50%) (TCID50) |
| p | 96-well microtitre plates containing 100 μL of confluent FRhK-4 were infected with 100 μL of serial 10-fold of dilutions of stock virus in minimal essential medium with 1% FCS (maintenance medium) starting from 10−1 to 108. Titrations were done in quadruplicate. Infected cells were incubated for 4 days at 37°C. Appearance of CPE was recorded daily. TCID50 was determined according to Reed and the Muench method [19]. |
| sec | 2.4. Effect of Drying, Heat, and Relative Humidity Ten microlitre of maintenance medium containing 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying. The experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated. |
| title | 2.4. Effect of Drying, Heat, and Relative Humidity |
| p | Ten microlitre of maintenance medium containing 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying. |
| p | The experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated. |
| sec | 2.5. Infectivity Assay The infectivity of residual virus was titrated in quadruplicate on 96-well microtitre plates containing 100 μL of confluent FRhK-4 cells. 100 μL of serial 10-fold of dilutions of virus in maintenance medium starting from 10−1 to 108 was added into FRhK-4 cells. The infected cells were incubated at 37°C for 4 days. Appearance of CPE was recorded daily. TCID50 was determined according to the Reed and Muench method [19]. |
| title | 2.5. Infectivity Assay |
| p | The infectivity of residual virus was titrated in quadruplicate on 96-well microtitre plates containing 100 μL of confluent FRhK-4 cells. 100 μL of serial 10-fold of dilutions of virus in maintenance medium starting from 10−1 to 108 was added into FRhK-4 cells. The infected cells were incubated at 37°C for 4 days. Appearance of CPE was recorded daily. TCID50 was determined according to the Reed and Muench method [19]. |
| sec | 3. Results Ten microlitre of 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plate (representing a nonporous surface) and dried. The dried virus was then incubated at different temperatures (38°C, 33°C, 28°C) at different relative humidity (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr and the residual viral infectivity was titrated. A similar experiment was conducted at room temperature and relative humidity of about 40–50% (air-conditioned room) for up to 4 weeks. Virus dried on plastic retained viability for up to 5 days at 22~25°C at relative humidity of 40~50% with only 1 log10 loss of titre (Figure 1). After that virus infectivity is gradually lost ever time. Loss of virus infectivity in solution was generally similar to dried virus under these environmental conditions. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites, especially in air-conditioned environments. High relative humidity (>95%) at comparatively low temperature (28°C and 33°C) did not affect the virus infectivity significantly (Figure 2(a)). High temperature (38°C) at 80–90% relative humidity led to a 0.25~2 log10 loss of titre at 24 hr (Figure 2(b)). However, if the dried virus was stored at high temperature (38°C) and high relative humidity (>95%), there was a further ~1.5 log loss of titre for each time point up to 24 hr (0.38~3.38 log10) when compared with high temperature (38°C) at a lower relative humidity 80–90% (Figures 3(a)–3(c)). |
| title | 3. Results |
| p | Ten microlitre of 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plate (representing a nonporous surface) and dried. The dried virus was then incubated at different temperatures (38°C, 33°C, 28°C) at different relative humidity (>95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr and the residual viral infectivity was titrated. A similar experiment was conducted at room temperature and relative humidity of about 40–50% (air-conditioned room) for up to 4 weeks. Virus dried on plastic retained viability for up to 5 days at 22~25°C at relative humidity of 40~50% with only 1 log10 loss of titre (Figure 1). After that virus infectivity is gradually lost ever time. Loss of virus infectivity in solution was generally similar to dried virus under these environmental conditions. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites, especially in air-conditioned environments. |
| p | High relative humidity (>95%) at comparatively low temperature (28°C and 33°C) did not affect the virus infectivity significantly (Figure 2(a)). High temperature (38°C) at 80–90% relative humidity led to a 0.25~2 log10 loss of titre at 24 hr (Figure 2(b)). However, if the dried virus was stored at high temperature (38°C) and high relative humidity (>95%), there was a further ~1.5 log loss of titre for each time point up to 24 hr (0.38~3.38 log10) when compared with high temperature (38°C) at a lower relative humidity 80–90% (Figures 3(a)–3(c)). |
| sec | 4. Discussion Viruses do not replicate outside living cell but infectious virus may persist on contaminated environmental surfaces and the duration of persistence of viable virus is affected markedly by temperature and humidity. Contaminated surfaces are known to be significant vectors in the transmission of infections in the hospital setting as well as the community. The role of fomites in the transmission of RSV has been clearly demonstrated [20]. Survival of viruses on a variety of fomites has been studied for influenza viruses, paramyxoviruses, poxviruses, and retroviruses [21]. The human coronavirus associated with the common cold was reported to remain viable only for 3 hours on environmental surfaces after drying, although it remains viable for many days in liquid suspension [13]. Parainfluenza and RSV viruses were viable after drying on surfaces for 2 and 6 hours, respectively [20, 22]. In aerosolised form, human coronavirus 229E is generally less stable in high humidity [12]. The environmental stability of SCoV was previously unknown and this information is clearly important for understanding the mechanisms of transmission of this virus in a hospital and community setting. In the present study, we have demonstrated that SARS CoV can survive at least two weeks after drying at temperature and humidity conditions found in an air-conditioned environment. The virus is stable for 3 weeks at room temperature in a liquid environment but it is easily killed by heat at 56°C for 15 minutes [9]. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites. These results may indicate that contaminated surfaces may play a major role in transmission of infection in the hospital and the community. Our studies indicate that SCoV is relatively more stable than the human coronaviruses 229E or OC43 and some other viral respiratory pathogens such as respiratory syncytial virus. These findings suggest that, while direct droplet transmission is an important route of transmission [23], the role of fomites and environmental contamination in virus transmission may play a significant role in virus transmission. In particular, fomites may contribute to the continued transmission of infection in the nosocomial setting that continues to occur in spite of the great attention and stringent precautions taken to prevent droplet spread. In addition to droplet precautions, reenforcing contact precautions and hand washing is called for. Faecal contamination of SCoV coronavirus may thus be an effective route of transmission of the disease. The outbreak in Amoy Garden in Hong Kong which affected over 300 residents in a single-apartment block with thought to have been transmitted by contaminated sewage. The stability of the virus on environmental surfaces and its presence in faeces indicates the potential that fecal contamination of fresh-food production may pose a threat for virus transmission; especially in countries with poor sanitation and sewage disposal systems and that studies to address this possibility are needed. In this study, we showed that high temperature at high relative humidity has a synergistic effect on inactivation of SARS CoV viability while lower temperatures and low humidity support prolonged survival of virus on contaminated surfaces. The environmental conditions of countries such as Malaysia, Indonesia, and Thailand are thus not conducive to the prolonged survival of the virus. In countries such as Singapore and Hong Kong where there is a intensive use of air-conditioning, transmission largely occurred in well-air-conditioned environments such as hospitals or hotels. Further, a separate study has shown that during the epidemic, the risk of increased daily incidence of SARS was 18.18-fold higher in days with a lower air temperature than in days with a higher temperature in Hong Kong [24] and other regions [15–17]. Taken together, these observations may explain why some Asian countries in tropical area (with high temperature at high relative humidity) such as Malaysia, Indonesia, and Thailand did not have nosocomial outbreaks of SARS (Tables 1 and 2(a)–2(c)). It may also explain why Singapore, which is also in tropical area (Table 2(d)), had most of its SARS outbreaks in hospitals (air-conditioned environment). Interestingly, during the outbreak of SARS in Guangzhou, clinicians kept the windows of patient rooms open and well ventilated and these may well have reduced virus survival and this reduced nosocomial transmission. SARS CoV can retain its infectivity up to 2 weeks at low temperature and low humidity environment, which might facilitate the virus transmission in community as in Hong Kong which locates in subtropical area (Table 2(e)). Other environmental factors including wind velocity, daily sunlight, and air pressure, had shown to be associated with SARS epidemic, should also be considered [16, 17]. The dynamics of SARS epidemic involves multiple factors including physical property of virus, outdoor and indoor environments, hygiene, space, and genetic predispositions [10, 15–17, 24, 25]. Understanding the stability of viruses in different temperature and humidity conditions is important in understanding transmission of novel infectious agent including that of the recent influenza Apandemic H1N12009. |
| title | 4. Discussion |
| p | Viruses do not replicate outside living cell but infectious virus may persist on contaminated environmental surfaces and the duration of persistence of viable virus is affected markedly by temperature and humidity. Contaminated surfaces are known to be significant vectors in the transmission of infections in the hospital setting as well as the community. The role of fomites in the transmission of RSV has been clearly demonstrated [20]. Survival of viruses on a variety of fomites has been studied for influenza viruses, paramyxoviruses, poxviruses, and retroviruses [21]. The human coronavirus associated with the common cold was reported to remain viable only for 3 hours on environmental surfaces after drying, although it remains viable for many days in liquid suspension [13]. Parainfluenza and RSV viruses were viable after drying on surfaces for 2 and 6 hours, respectively [20, 22]. In aerosolised form, human coronavirus 229E is generally less stable in high humidity [12]. The environmental stability of SCoV was previously unknown and this information is clearly important for understanding the mechanisms of transmission of this virus in a hospital and community setting. |
| p | In the present study, we have demonstrated that SARS CoV can survive at least two weeks after drying at temperature and humidity conditions found in an air-conditioned environment. The virus is stable for 3 weeks at room temperature in a liquid environment but it is easily killed by heat at 56°C for 15 minutes [9]. This indicates that SARS CoV is a stable virus that may potentially be transmitted by indirect contact or fomites. These results may indicate that contaminated surfaces may play a major role in transmission of infection in the hospital and the community. |
| p | Our studies indicate that SCoV is relatively more stable than the human coronaviruses 229E or OC43 and some other viral respiratory pathogens such as respiratory syncytial virus. These findings suggest that, while direct droplet transmission is an important route of transmission [23], the role of fomites and environmental contamination in virus transmission may play a significant role in virus transmission. In particular, fomites may contribute to the continued transmission of infection in the nosocomial setting that continues to occur in spite of the great attention and stringent precautions taken to prevent droplet spread. In addition to droplet precautions, reenforcing contact precautions and hand washing is called for. |
| p | Faecal contamination of SCoV coronavirus may thus be an effective route of transmission of the disease. The outbreak in Amoy Garden in Hong Kong which affected over 300 residents in a single-apartment block with thought to have been transmitted by contaminated sewage. The stability of the virus on environmental surfaces and its presence in faeces indicates the potential that fecal contamination of fresh-food production may pose a threat for virus transmission; especially in countries with poor sanitation and sewage disposal systems and that studies to address this possibility are needed. |
| p | In this study, we showed that high temperature at high relative humidity has a synergistic effect on inactivation of SARS CoV viability while lower temperatures and low humidity support prolonged survival of virus on contaminated surfaces. The environmental conditions of countries such as Malaysia, Indonesia, and Thailand are thus not conducive to the prolonged survival of the virus. In countries such as Singapore and Hong Kong where there is a intensive use of air-conditioning, transmission largely occurred in well-air-conditioned environments such as hospitals or hotels. Further, a separate study has shown that during the epidemic, the risk of increased daily incidence of SARS was 18.18-fold higher in days with a lower air temperature than in days with a higher temperature in Hong Kong [24] and other regions [15–17]. Taken together, these observations may explain why some Asian countries in tropical area (with high temperature at high relative humidity) such as Malaysia, Indonesia, and Thailand did not have nosocomial outbreaks of SARS (Tables 1 and 2(a)–2(c)). It may also explain why Singapore, which is also in tropical area (Table 2(d)), had most of its SARS outbreaks in hospitals (air-conditioned environment). Interestingly, during the outbreak of SARS in Guangzhou, clinicians kept the windows of patient rooms open and well ventilated and these may well have reduced virus survival and this reduced nosocomial transmission. SARS CoV can retain its infectivity up to 2 weeks at low temperature and low humidity environment, which might facilitate the virus transmission in community as in Hong Kong which locates in subtropical area (Table 2(e)). Other environmental factors including wind velocity, daily sunlight, and air pressure, had shown to be associated with SARS epidemic, should also be considered [16, 17]. The dynamics of SARS epidemic involves multiple factors including physical property of virus, outdoor and indoor environments, hygiene, space, and genetic predispositions [10, 15–17, 24, 25]. Understanding the stability of viruses in different temperature and humidity conditions is important in understanding transmission of novel infectious agent including that of the recent influenza Apandemic H1N12009. |
| back | Conflict of Interests The authors declare that there is no conflict of interest. |
| ack | Conflict of Interests The authors declare that there is no conflict of interest. |
| title | Conflict of Interests |
| p | The authors declare that there is no conflict of interest. |
| figure | Figure 1 Residual virus infectivity at 22–25°C with relative humidity 40–50% (starting titre 105/10 μL) and at 33°C or 38°C with relative humidity >95%. |
| label | Figure 1 |
| caption | Residual virus infectivity at 22–25°C with relative humidity 40–50% (starting titre 105/10 μL) and at 33°C or 38°C with relative humidity >95%. |
| p | Residual virus infectivity at 22–25°C with relative humidity 40–50% (starting titre 105/10 μL) and at 33°C or 38°C with relative humidity >95%. |
| figure | Figure 2 Infectivity of SARS Coronavirus (105/10 μL) to different temperatures at (a) >95% relative humidity, (b) >80–89%. |
| label | Figure 2 |
| caption | Infectivity of SARS Coronavirus (105/10 μL) to different temperatures at (a) >95% relative humidity, (b) >80–89%. |
| p | Infectivity of SARS Coronavirus (105/10 μL) to different temperatures at (a) >95% relative humidity, (b) >80–89%. |
| figure | Figure 3 Infectivity of SARS Coronavirus (starting titre 105/10 μL) at different relative humidity at (a) 38°C, (b) 33°C, and (c) 28°C. |
| label | Figure 3 |
| caption | Infectivity of SARS Coronavirus (starting titre 105/10 μL) at different relative humidity at (a) 38°C, (b) 33°C, and (c) 28°C. |
| p | Infectivity of SARS Coronavirus (starting titre 105/10 μL) at different relative humidity at (a) 38°C, (b) 33°C, and (c) 28°C. |
| table-wrap | Table 1 WHO SARS report—based on data as of the 31st December 2003. Areas Total Medan age Deaths Case fatality Ratio (%) No. of imported Cases (%) No. of HCW (%) First case Last case China 5327 NKn 349 7 NA 1002 (19) Nov-02 Jun-03 Hong Kong 1755 40 299 17 NA 386 (22) Feb-03 May-03 Taiwan 346 42 37 11 21 (6) 68 (20) Feb-03 Jun-03 Singapore 238 35 33 14 8 (3) 97 (41) Feb-03 May-03 Viet Nam 63 43 5 8 1 (2) 36 (57) Feb-03 Apr-03 Indonesia 2 56 0 0 2 (100) 0 (0) Apr-03 Apr-03 Malaysia 5 30 2 40 5 (100) 0 (0) Mar-03 Apr-03 Thailand 9 42 2 22 9 (100) 1 (11) Mar-03 May-03 Philippines 14 41 2 14 7 (50) 4 (29) Feb-03 May-03 Total 8096 774 9.6 142 1706 (21) |
| label | Table 1 |
| caption | WHO SARS report—based on data as of the 31st December 2003. |
| p | WHO SARS report—based on data as of the 31st December 2003. |
| table | Areas Total Medan age Deaths Case fatality Ratio (%) No. of imported Cases (%) No. of HCW (%) First case Last case China 5327 NKn 349 7 NA 1002 (19) Nov-02 Jun-03 Hong Kong 1755 40 299 17 NA 386 (22) Feb-03 May-03 Taiwan 346 42 37 11 21 (6) 68 (20) Feb-03 Jun-03 Singapore 238 35 33 14 8 (3) 97 (41) Feb-03 May-03 Viet Nam 63 43 5 8 1 (2) 36 (57) Feb-03 Apr-03 Indonesia 2 56 0 0 2 (100) 0 (0) Apr-03 Apr-03 Malaysia 5 30 2 40 5 (100) 0 (0) Mar-03 Apr-03 Thailand 9 42 2 22 9 (100) 1 (11) Mar-03 May-03 Philippines 14 41 2 14 7 (50) 4 (29) Feb-03 May-03 Total 8096 774 9.6 142 1706 (21) |
| tr | Areas Total Medan age Deaths Case fatality Ratio (%) No. of imported Cases (%) No. of HCW (%) First case Last case |
| th | Areas |
| th | Total |
| th | Medan age |
| th | Deaths |
| th | Case fatality Ratio (%) |
| th | No. of imported Cases (%) |
| th | No. of HCW (%) |
| th | First case |
| th | Last case |
| tr | China 5327 NKn 349 7 NA 1002 (19) Nov-02 Jun-03 |
| td | China |
| td | 5327 |
| td | NKn |
| td | 349 |
| td | 7 |
| td | NA |
| td | 1002 (19) |
| td | Nov-02 |
| td | Jun-03 |
| tr | Hong Kong 1755 40 299 17 NA 386 (22) Feb-03 May-03 |
| td | Hong Kong |
| td | 1755 |
| td | 40 |
| td | 299 |
| td | 17 |
| td | NA |
| td | 386 (22) |
| td | Feb-03 |
| td | May-03 |
| tr | Taiwan 346 42 37 11 21 (6) 68 (20) Feb-03 Jun-03 |
| td | Taiwan |
| td | 346 |
| td | 42 |
| td | 37 |
| td | 11 |
| td | 21 (6) |
| td | 68 (20) |
| td | Feb-03 |
| td | Jun-03 |
| tr | Singapore 238 35 33 14 8 (3) 97 (41) Feb-03 May-03 |
| td | Singapore |
| td | 238 |
| td | 35 |
| td | 33 |
| td | 14 |
| td | 8 (3) |
| td | 97 (41) |
| td | Feb-03 |
| td | May-03 |
| tr | Viet Nam 63 43 5 8 1 (2) 36 (57) Feb-03 Apr-03 |
| td | Viet Nam |
| td | 63 |
| td | 43 |
| td | 5 |
| td | 8 |
| td | 1 (2) |
| td | 36 (57) |
| td | Feb-03 |
| td | Apr-03 |
| tr | Indonesia 2 56 0 0 2 (100) 0 (0) Apr-03 Apr-03 |
| td | Indonesia |
| td | 2 |
| td | 56 |
| td | 0 |
| td | 0 |
| td | 2 (100) |
| td | 0 (0) |
| td | Apr-03 |
| td | Apr-03 |
| tr | Malaysia 5 30 2 40 5 (100) 0 (0) Mar-03 Apr-03 |
| td | Malaysia |
| td | 5 |
| td | 30 |
| td | 2 |
| td | 40 |
| td | 5 (100) |
| td | 0 (0) |
| td | Mar-03 |
| td | Apr-03 |
| tr | Thailand 9 42 2 22 9 (100) 1 (11) Mar-03 May-03 |
| td | Thailand |
| td | 9 |
| td | 42 |
| td | 2 |
| td | 22 |
| td | 9 (100) |
| td | 1 (11) |
| td | Mar-03 |
| td | May-03 |
| tr | Philippines 14 41 2 14 7 (50) 4 (29) Feb-03 May-03 |
| td | Philippines |
| td | 14 |
| td | 41 |
| td | 2 |
| td | 14 |
| td | 7 (50) |
| td | 4 (29) |
| td | Feb-03 |
| td | May-03 |
| tr | Total 8096 774 9.6 142 1706 (21) |
| td | Total |
| td | 8096 |
| td | 774 |
| td | 9.6 |
| td | 142 |
| td | 1706 (21) |
| table-wrap | Table 2 A summary of the meteorological data of 2005 in average weather conditions*. Month Average sunlight (hours) Temperature Discomfort from heat and humidity Relative humidity Min Max am pm (a) Kuala Lumpur, Malaysia Jan 6 22 32 High 97 60 Feb 7 22 33 High 97 60 March 7 23 33 High 97 58 April 6 23 33 High 97 63 May 6 23 33 High 97 66 June 7 22 33 High 96 63 July 7 23 32 High 95 63 Aug 6 23 32 High 96 62 Sept 6 23 32 High 96 64 Oct 5 23 32 High 96 65 Nov 5 23 32 High 97 66 Dec 5 22 32 High 97 61 (b) Jakarta, Indonesia Jan 5 23 29 High 95 75 Feb 5 23 29 High 95 75 March 6 23 30 High 94 73 April 7 24 31 High 94 71 May 7 24 31 High 94 69 June 7 23 31 High 93 67 July 7 23 31 High 92 64 Aug 8 23 31 High 90 61 Sept 8 23 31 High 90 62 Oct 7 23 31 High 90 64 Nov 6 23 30 High 92 68 Dec 5 23 29 High 92 71 (c) Bangkok, Thailand Jan 9 20 32 High 91 53 Feb 8 22 33 High 92 55 March 9 24 34 High 92 56 April 8 25 35 Extreme 90 58 May 8 25 34 Extreme 91 64 June 6 24 33 Extreme 90 67 July 5 24 32 High 91 66 Aug 5 24 32 High 92 66 Sept 5 24 32 High 94 70 Oct 6 24 31 High 93 70 Nov 8 22 31 High 92 65 Dec 9 20 31 High 91 56 (d) Singapore Jan 5 23 30 High 82 78 Feb 7 23 31 High 77 71 March 6 24 31 High 76 70 April 6 24 31 High 77 74 May 6 24 32 Extreme 79 73 June 6 24 31 High 79 73 July 6 24 31 High 79 72 Aug 6 24 31 High 78 72 Sept 5 24 31 High 79 72 Oct 5 23 31 High 78 72 Nov 5 23 31 High 79 75 Dec 4 23 31 High 82 78 (e) Hong Kong Jan 5 13 18 — 77 66 Feb 4 13 17 — 82 73 March 3 16 19 — 84 74 April 4 19 24 Medium 87 77 May 5 23 28 Medium 87 78 June 5 26 29 High 86 77 July 8 26 31 High 87 77 Aug 6 26 31 High 87 77 Sept 6 25 29 High 83 72 Oct 7 23 27 Medium 75 63 Nov 7 18 23 Moderate 73 60 Dec 6 15 20 — 74 63 *Data is available at BBC weather website (http://www.bbc.co.uk/weather/world/city_guides/results). |
| label | Table 2 |
| caption | A summary of the meteorological data of 2005 in average weather conditions*. |
| p | A summary of the meteorological data of 2005 in average weather conditions*. |
| table | Month Average sunlight (hours) Temperature Discomfort from heat and humidity Relative humidity Min Max am pm (a) Kuala Lumpur, Malaysia Jan 6 22 32 High 97 60 Feb 7 22 33 High 97 60 March 7 23 33 High 97 58 April 6 23 33 High 97 63 May 6 23 33 High 97 66 June 7 22 33 High 96 63 July 7 23 32 High 95 63 Aug 6 23 32 High 96 62 Sept 6 23 32 High 96 64 Oct 5 23 32 High 96 65 Nov 5 23 32 High 97 66 Dec 5 22 32 High 97 61 (b) Jakarta, Indonesia Jan 5 23 29 High 95 75 Feb 5 23 29 High 95 75 March 6 23 30 High 94 73 April 7 24 31 High 94 71 May 7 24 31 High 94 69 June 7 23 31 High 93 67 July 7 23 31 High 92 64 Aug 8 23 31 High 90 61 Sept 8 23 31 High 90 62 Oct 7 23 31 High 90 64 Nov 6 23 30 High 92 68 Dec 5 23 29 High 92 71 (c) Bangkok, Thailand Jan 9 20 32 High 91 53 Feb 8 22 33 High 92 55 March 9 24 34 High 92 56 April 8 25 35 Extreme 90 58 May 8 25 34 Extreme 91 64 June 6 24 33 Extreme 90 67 July 5 24 32 High 91 66 Aug 5 24 32 High 92 66 Sept 5 24 32 High 94 70 Oct 6 24 31 High 93 70 Nov 8 22 31 High 92 65 Dec 9 20 31 High 91 56 (d) Singapore Jan 5 23 30 High 82 78 Feb 7 23 31 High 77 71 March 6 24 31 High 76 70 April 6 24 31 High 77 74 May 6 24 32 Extreme 79 73 June 6 24 31 High 79 73 July 6 24 31 High 79 72 Aug 6 24 31 High 78 72 Sept 5 24 31 High 79 72 Oct 5 23 31 High 78 72 Nov 5 23 31 High 79 75 Dec 4 23 31 High 82 78 (e) Hong Kong Jan 5 13 18 — 77 66 Feb 4 13 17 — 82 73 March 3 16 19 — 84 74 April 4 19 24 Medium 87 77 May 5 23 28 Medium 87 78 June 5 26 29 High 86 77 July 8 26 31 High 87 77 Aug 6 26 31 High 87 77 Sept 6 25 29 High 83 72 Oct 7 23 27 Medium 75 63 Nov 7 18 23 Moderate 73 60 Dec 6 15 20 — 74 63 |
| tr | Month Average sunlight (hours) Temperature Discomfort from heat and humidity Relative humidity |
| th | Month |
| th | Average sunlight (hours) |
| th | Temperature |
| th | Discomfort from heat and humidity |
| th | Relative humidity |
| tr | Min Max am pm |
| th | Min |
| th | Max |
| th | am |
| th | pm |
| tr | (a) Kuala Lumpur, Malaysia |
| td | (a) Kuala Lumpur, Malaysia |
| tr | Jan 6 22 32 High 97 60 |
| td | Jan |
| td | 6 |
| td | 22 |
| td | 32 |
| td | High |
| td | 97 |
| td | 60 |
| tr | Feb 7 22 33 High 97 60 |
| td | Feb |
| td | 7 |
| td | 22 |
| td | 33 |
| td | High |
| td | 97 |
| td | 60 |
| tr | March 7 23 33 High 97 58 |
| td | March |
| td | 7 |
| td | 23 |
| td | 33 |
| td | High |
| td | 97 |
| td | 58 |
| tr | April 6 23 33 High 97 63 |
| td | April |
| td | 6 |
| td | 23 |
| td | 33 |
| td | High |
| td | 97 |
| td | 63 |
| tr | May 6 23 33 High 97 66 |
| td | May |
| td | 6 |
| td | 23 |
| td | 33 |
| td | High |
| td | 97 |
| td | 66 |
| tr | June 7 22 33 High 96 63 |
| td | June |
| td | 7 |
| td | 22 |
| td | 33 |
| td | High |
| td | 96 |
| td | 63 |
| tr | July 7 23 32 High 95 63 |
| td | July |
| td | 7 |
| td | 23 |
| td | 32 |
| td | High |
| td | 95 |
| td | 63 |
| tr | Aug 6 23 32 High 96 62 |
| td | Aug |
| td | 6 |
| td | 23 |
| td | 32 |
| td | High |
| td | 96 |
| td | 62 |
| tr | Sept 6 23 32 High 96 64 |
| td | Sept |
| td | 6 |
| td | 23 |
| td | 32 |
| td | High |
| td | 96 |
| td | 64 |
| tr | Oct 5 23 32 High 96 65 |
| td | Oct |
| td | 5 |
| td | 23 |
| td | 32 |
| td | High |
| td | 96 |
| td | 65 |
| tr | Nov 5 23 32 High 97 66 |
| td | Nov |
| td | 5 |
| td | 23 |
| td | 32 |
| td | High |
| td | 97 |
| td | 66 |
| tr | Dec 5 22 32 High 97 61 |
| td | Dec |
| td | 5 |
| td | 22 |
| td | 32 |
| td | High |
| td | 97 |
| td | 61 |
| tr | (b) Jakarta, Indonesia |
| td | (b) Jakarta, Indonesia |
| tr | Jan 5 23 29 High 95 75 |
| td | Jan |
| td | 5 |
| td | 23 |
| td | 29 |
| td | High |
| td | 95 |
| td | 75 |
| tr | Feb 5 23 29 High 95 75 |
| td | Feb |
| td | 5 |
| td | 23 |
| td | 29 |
| td | High |
| td | 95 |
| td | 75 |
| tr | March 6 23 30 High 94 73 |
| td | March |
| td | 6 |
| td | 23 |
| td | 30 |
| td | High |
| td | 94 |
| td | 73 |
| tr | April 7 24 31 High 94 71 |
| td | April |
| td | 7 |
| td | 24 |
| td | 31 |
| td | High |
| td | 94 |
| td | 71 |
| tr | May 7 24 31 High 94 69 |
| td | May |
| td | 7 |
| td | 24 |
| td | 31 |
| td | High |
| td | 94 |
| td | 69 |
| tr | June 7 23 31 High 93 67 |
| td | June |
| td | 7 |
| td | 23 |
| td | 31 |
| td | High |
| td | 93 |
| td | 67 |
| tr | July 7 23 31 High 92 64 |
| td | July |
| td | 7 |
| td | 23 |
| td | 31 |
| td | High |
| td | 92 |
| td | 64 |
| tr | Aug 8 23 31 High 90 61 |
| td | Aug |
| td | 8 |
| td | 23 |
| td | 31 |
| td | High |
| td | 90 |
| td | 61 |
| tr | Sept 8 23 31 High 90 62 |
| td | Sept |
| td | 8 |
| td | 23 |
| td | 31 |
| td | High |
| td | 90 |
| td | 62 |
| tr | Oct 7 23 31 High 90 64 |
| td | Oct |
| td | 7 |
| td | 23 |
| td | 31 |
| td | High |
| td | 90 |
| td | 64 |
| tr | Nov 6 23 30 High 92 68 |
| td | Nov |
| td | 6 |
| td | 23 |
| td | 30 |
| td | High |
| td | 92 |
| td | 68 |
| tr | Dec 5 23 29 High 92 71 |
| td | Dec |
| td | 5 |
| td | 23 |
| td | 29 |
| td | High |
| td | 92 |
| td | 71 |
| tr | (c) Bangkok, Thailand |
| td | (c) Bangkok, Thailand |
| tr | Jan 9 20 32 High 91 53 |
| td | Jan |
| td | 9 |
| td | 20 |
| td | 32 |
| td | High |
| td | 91 |
| td | 53 |
| tr | Feb 8 22 33 High 92 55 |
| td | Feb |
| td | 8 |
| td | 22 |
| td | 33 |
| td | High |
| td | 92 |
| td | 55 |
| tr | March 9 24 34 High 92 56 |
| td | March |
| td | 9 |
| td | 24 |
| td | 34 |
| td | High |
| td | 92 |
| td | 56 |
| tr | April 8 25 35 Extreme 90 58 |
| td | April |
| td | 8 |
| td | 25 |
| td | 35 |
| td | Extreme |
| td | 90 |
| td | 58 |
| tr | May 8 25 34 Extreme 91 64 |
| td | May |
| td | 8 |
| td | 25 |
| td | 34 |
| td | Extreme |
| td | 91 |
| td | 64 |
| tr | June 6 24 33 Extreme 90 67 |
| td | June |
| td | 6 |
| td | 24 |
| td | 33 |
| td | Extreme |
| td | 90 |
| td | 67 |
| tr | July 5 24 32 High 91 66 |
| td | July |
| td | 5 |
| td | 24 |
| td | 32 |
| td | High |
| td | 91 |
| td | 66 |
| tr | Aug 5 24 32 High 92 66 |
| td | Aug |
| td | 5 |
| td | 24 |
| td | 32 |
| td | High |
| td | 92 |
| td | 66 |
| tr | Sept 5 24 32 High 94 70 |
| td | Sept |
| td | 5 |
| td | 24 |
| td | 32 |
| td | High |
| td | 94 |
| td | 70 |
| tr | Oct 6 24 31 High 93 70 |
| td | Oct |
| td | 6 |
| td | 24 |
| td | 31 |
| td | High |
| td | 93 |
| td | 70 |
| tr | Nov 8 22 31 High 92 65 |
| td | Nov |
| td | 8 |
| td | 22 |
| td | 31 |
| td | High |
| td | 92 |
| td | 65 |
| tr | Dec 9 20 31 High 91 56 |
| td | Dec |
| td | 9 |
| td | 20 |
| td | 31 |
| td | High |
| td | 91 |
| td | 56 |
| tr | (d) Singapore |
| td | (d) Singapore |
| tr | Jan 5 23 30 High 82 78 |
| td | Jan |
| td | 5 |
| td | 23 |
| td | 30 |
| td | High |
| td | 82 |
| td | 78 |
| tr | Feb 7 23 31 High 77 71 |
| td | Feb |
| td | 7 |
| td | 23 |
| td | 31 |
| td | High |
| td | 77 |
| td | 71 |
| tr | March 6 24 31 High 76 70 |
| td | March |
| td | 6 |
| td | 24 |
| td | 31 |
| td | High |
| td | 76 |
| td | 70 |
| tr | April 6 24 31 High 77 74 |
| td | April |
| td | 6 |
| td | 24 |
| td | 31 |
| td | High |
| td | 77 |
| td | 74 |
| tr | May 6 24 32 Extreme 79 73 |
| td | May |
| td | 6 |
| td | 24 |
| td | 32 |
| td | Extreme |
| td | 79 |
| td | 73 |
| tr | June 6 24 31 High 79 73 |
| td | June |
| td | 6 |
| td | 24 |
| td | 31 |
| td | High |
| td | 79 |
| td | 73 |
| tr | July 6 24 31 High 79 72 |
| td | July |
| td | 6 |
| td | 24 |
| td | 31 |
| td | High |
| td | 79 |
| td | 72 |
| tr | Aug 6 24 31 High 78 72 |
| td | Aug |
| td | 6 |
| td | 24 |
| td | 31 |
| td | High |
| td | 78 |
| td | 72 |
| tr | Sept 5 24 31 High 79 72 |
| td | Sept |
| td | 5 |
| td | 24 |
| td | 31 |
| td | High |
| td | 79 |
| td | 72 |
| tr | Oct 5 23 31 High 78 72 |
| td | Oct |
| td | 5 |
| td | 23 |
| td | 31 |
| td | High |
| td | 78 |
| td | 72 |
| tr | Nov 5 23 31 High 79 75 |
| td | Nov |
| td | 5 |
| td | 23 |
| td | 31 |
| td | High |
| td | 79 |
| td | 75 |
| tr | Dec 4 23 31 High 82 78 |
| td | Dec |
| td | 4 |
| td | 23 |
| td | 31 |
| td | High |
| td | 82 |
| td | 78 |
| tr | (e) Hong Kong |
| td | (e) Hong Kong |
| tr | Jan 5 13 18 — 77 66 |
| td | Jan |
| td | 5 |
| td | 13 |
| td | 18 |
| td | — |
| td | 77 |
| td | 66 |
| tr | Feb 4 13 17 — 82 73 |
| td | Feb |
| td | 4 |
| td | 13 |
| td | 17 |
| td | — |
| td | 82 |
| td | 73 |
| tr | March 3 16 19 — 84 74 |
| td | March |
| td | 3 |
| td | 16 |
| td | 19 |
| td | — |
| td | 84 |
| td | 74 |
| tr | April 4 19 24 Medium 87 77 |
| td | April |
| td | 4 |
| td | 19 |
| td | 24 |
| td | Medium |
| td | 87 |
| td | 77 |
| tr | May 5 23 28 Medium 87 78 |
| td | May |
| td | 5 |
| td | 23 |
| td | 28 |
| td | Medium |
| td | 87 |
| td | 78 |
| tr | June 5 26 29 High 86 77 |
| td | June |
| td | 5 |
| td | 26 |
| td | 29 |
| td | High |
| td | 86 |
| td | 77 |
| tr | July 8 26 31 High 87 77 |
| td | July |
| td | 8 |
| td | 26 |
| td | 31 |
| td | High |
| td | 87 |
| td | 77 |
| tr | Aug 6 26 31 High 87 77 |
| td | Aug |
| td | 6 |
| td | 26 |
| td | 31 |
| td | High |
| td | 87 |
| td | 77 |
| tr | Sept 6 25 29 High 83 72 |
| td | Sept |
| td | 6 |
| td | 25 |
| td | 29 |
| td | High |
| td | 83 |
| td | 72 |
| tr | Oct 7 23 27 Medium 75 63 |
| td | Oct |
| td | 7 |
| td | 23 |
| td | 27 |
| td | Medium |
| td | 75 |
| td | 63 |
| tr | Nov 7 18 23 Moderate 73 60 |
| td | Nov |
| td | 7 |
| td | 18 |
| td | 23 |
| td | Moderate |
| td | 73 |
| td | 60 |
| tr | Dec 6 15 20 — 74 63 |
| td | Dec |
| td | 6 |
| td | 15 |
| td | 20 |
| td | — |
| td | 74 |
| td | 63 |
| table-wrap-foot | *Data is available at BBC weather website (http://www.bbc.co.uk/weather/world/city_guides/results). |
| footnote | *Data is available at BBC weather website (http://www.bbc.co.uk/weather/world/city_guides/results). |
| p | *Data is available at BBC weather website (http://www.bbc.co.uk/weather/world/city_guides/results). |
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