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Background: Collection efficiency CE of an apharesis machine refers to the proportion of cells passing through it that is harvested. A higher collection efficiency reduces the need for repeated apharesis sessions and is a good measure for comparison of individual machines. Methods: Patients ho underent first harvest after G-CSF mobilization beteen Jan 2014 and Sept 2015 ere included in the study. G-CSF as given at 10mcgkgday for 4 days and harvest as started from d5. Peripheral blood CD34 PBCD34 counts ere enumerated on d4. The 3 apharesis machines used ere Haemonetics MCS+, Fresenius Com.Tec and Cobe Spectra. Standard volume apharesis 2-3 times patient blood volume ith recommended MNC protocols ere employed. CE as percent is calculated as total number of CD34+ cells collectedxD7;10-4 divided by D4-PBCD34xB5;LxD7;blood vol. processed lt . Oneay ANOVA ith post hoc analysis as used to compare CE of individual machines. Simple logistic regression as used to look for peri-apharesis factors hich could have influenced CE. Results: Of 141 patients, 94 had multiple myeloma, 16 ere NHL, 15 ere HL and 16 patients had other malignancies. Comparison of individual machines by CE as depicted in table. CE as significantly influenced by peri-apharesis factors such as circulating CD34+ cells as assessed by D4-PBCD34 count , TLC and flo rate of the machine. CE had a inverse relation ith all these factors. It remained unaffected by volume of blood processed. The mean time taken for harvest completion as higher for Haemonetics 326 min 64 in comparison to both Fresenius 241 min 35; plt0.001 and Cobe Spectra 239 min 36; plt0.001 . Conclusions: In terms of collection efficiency, the performance of Haemonetics MCS+ is better on comparison ith both Cobe Spectra and Fresenius Com.Tec, though statistically significant in former. This benefit is offset by prolonged procedure time required for this machine. MachineHaemonetics MCS+ n=36 Fresenius Com.Tec n=51 Cobe Spectra n=54 CE Mean SD 43.3 19.1 37.8 14.8 31.7 14.3 Post hoc analysisHaemonetics vs FreseniusFresenius vs Cobe SpectraHaemonetics vs Cobe SpectraMean reduction in CE p value -5.5 0.35 -6.1 0.15 -11.6 0.003 ,J Clin Oncol 34, 2016 suppl; abstr 8024 00:00.0,Hematologic MalignanciesPlasma Cell Dyscrasia
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