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Background: Nasopharyngeal carcinoma NPC is one of the most common malignant tumors ith poor prognosis and recurrence in South China. The hard eradication of NPC in clinic is predominantly due to cancer stem cells CSCs . Increasing evidence revealed that the aberrant activation of Wntx3B2;-catenin as positively correlated ith the produce of CSCs. Methods: To further investigate the effect of x3B2;-catenin on CSCs and tumorigenesis in NPC, a CNE2 cell line pLKO.1-sh-x3B2;-catenin-CNE2 ith stably suppressed expression of x3B2;-catenin as used in this study. The expressions of biomarkers in CSCs including c-myc, Nanog, Oct34, Sox2, EpCAM, as ell as adhesion-related proteins like E-cadherin and vimentin ere analyzed by estern blot analysis and immunofluorescent staining. The proliferation and migration abilities ere investigated by MTT assay and transell assay, respectively. Cell cycle as analyzed by flo cytometry. Finally, xenograft as performed to determine the effect of x3B2;-catenin on oncogenesis in vivo. Results: Results shoed that the expressions of c-myc, Nanog, Oct34, Sox2, and EpCAM ere all decreased in pLKO.1-sh-x3B2;-catenin-CNE2 cells. It as also found that vimentin as don-regulated, hile E-cadherin as upregulated. Results of MTT and transell assays suggested that the proliferation and migration abilities ere impaired by silencing of x3B2;-catenin, and more cells ere arrested in G1 phase hen compared ith the control. In vivo study indicated that the tumor groth as markedly suppressed in experimental group. Conclusions: Based on current findings, x3B2;-catenin may function as an essential protein for the maintenance of migration and proliferation abilities of NPC cells, and a complicated netork consisting of c-myc, Nanog, Oct34, Sox2, EpCAM, E-cadherin, vimentin and x3B2;-catenin may be involved in the inherent regulation mechanisms.,J Clin Oncol 34, 2016 suppl; abstr e17532 ,Publication Only Head and Neck Cancer
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