PubMed:9884411 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/9884411","sourcedb":"PubMed","sourceid":"9884411","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/9884411","text":"A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme.\nA full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. XEG hydrolyzes its substrates with retention of the anomeric configuration. The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans.","tracks":[{"project":"GlyCosmos6-UBERON","denotations":[{"id":"T1","span":{"begin":997,"end":1001},"obj":"Body_part"},{"id":"T2","span":{"begin":1002,"end":1006},"obj":"Body_part"},{"id":"T3","span":{"begin":1380,"end":1384},"obj":"Body_part"},{"id":"T4","span":{"begin":1429,"end":1433},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000060"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000000"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-UBERON"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-UBERON"}]},{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":2,"end":12},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T2","span":{"begin":195,"end":205},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T3","span":{"begin":454,"end":464},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T4","span":{"begin":939,"end":950},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T5","span":{"begin":1051,"end":1061},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T6","span":{"begin":1341,"end":1351},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T7","span":{"begin":1461,"end":1471},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T8","span":{"begin":1574,"end":1585},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycan-Motif"},{"subj":"T2","pred":"source","obj":"Glycan-Motif"},{"subj":"T3","pred":"source","obj":"Glycan-Motif"},{"subj":"T4","pred":"source","obj":"Glycan-Motif"},{"subj":"T5","pred":"source","obj":"Glycan-Motif"},{"subj":"T6","pred":"source","obj":"Glycan-Motif"},{"subj":"T7","pred":"source","obj":"Glycan-Motif"},{"subj":"T8","pred":"source","obj":"Glycan-Motif"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":2,"end":12},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":195,"end":205},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":454,"end":464},"obj":"Glycan_Motif"},{"id":"T4","span":{"begin":939,"end":950},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":1051,"end":1061},"obj":"Glycan_Motif"},{"id":"T6","span":{"begin":1341,"end":1351},"obj":"Glycan_Motif"},{"id":"T7","span":{"begin":1461,"end":1471},"obj":"Glycan_Motif"},{"id":"T8","span":{"begin":1574,"end":1585},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"id":"A4","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"id":"A6","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"id":"A7","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"id":"A8","pred":"image","subj":"T8","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T6","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T7","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T8","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":2,"end":12},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"T2","span":{"begin":195,"end":205},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"T3","span":{"begin":454,"end":464},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"T4","span":{"begin":1051,"end":1061},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"T5","span":{"begin":1341,"end":1351},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"T6","span":{"begin":1461,"end":1471},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T2","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T3","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T4","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T5","pred":"source","obj":"Glycosmos6-GlycoEpitope"},{"subj":"T6","pred":"source","obj":"Glycosmos6-GlycoEpitope"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":2,"end":12},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T2","span":{"begin":195,"end":205},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T3","span":{"begin":454,"end":464},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T4","span":{"begin":939,"end":950},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T5","span":{"begin":1051,"end":1061},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T6","span":{"begin":1341,"end":1351},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T7","span":{"begin":1461,"end":1471},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"},{"id":"T8","span":{"begin":1574,"end":1585},"obj":"https://glytoucan.org/Structures/Glycans/G25326UC"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T5","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T6","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T7","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T8","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":163},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":164,"end":346},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":347,"end":465},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":466,"end":627},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":628,"end":740},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":741,"end":895},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":896,"end":1104},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1105,"end":1180},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1181,"end":1288},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1289,"end":1391},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1392,"end":1586},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":163},"obj":"Sentence"},{"id":"T2","span":{"begin":164,"end":346},"obj":"Sentence"},{"id":"T3","span":{"begin":347,"end":465},"obj":"Sentence"},{"id":"T4","span":{"begin":466,"end":627},"obj":"Sentence"},{"id":"T5","span":{"begin":628,"end":740},"obj":"Sentence"},{"id":"T6","span":{"begin":741,"end":895},"obj":"Sentence"},{"id":"T7","span":{"begin":896,"end":1104},"obj":"Sentence"},{"id":"T8","span":{"begin":1105,"end":1180},"obj":"Sentence"},{"id":"T9","span":{"begin":1181,"end":1288},"obj":"Sentence"},{"id":"T10","span":{"begin":1289,"end":1391},"obj":"Sentence"},{"id":"T11","span":{"begin":1392,"end":1586},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":163},"obj":"Sentence"},{"id":"T2","span":{"begin":164,"end":346},"obj":"Sentence"},{"id":"T3","span":{"begin":347,"end":465},"obj":"Sentence"},{"id":"T4","span":{"begin":466,"end":627},"obj":"Sentence"},{"id":"T5","span":{"begin":628,"end":740},"obj":"Sentence"},{"id":"T6","span":{"begin":741,"end":895},"obj":"Sentence"},{"id":"T7","span":{"begin":896,"end":1104},"obj":"Sentence"},{"id":"T8","span":{"begin":1105,"end":1180},"obj":"Sentence"},{"id":"T9","span":{"begin":1181,"end":1288},"obj":"Sentence"},{"id":"T10","span":{"begin":1289,"end":1391},"obj":"Sentence"},{"id":"T11","span":{"begin":1392,"end":1586},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T11","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":51,"end":72},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":293,"end":314},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":528,"end":539},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":540,"end":557},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":580,"end":598},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"NCBItxid:5053"},{"id":"A2","pred":"db_id","subj":"T2","obj":"NCBItxid:5053"},{"id":"A3","pred":"db_id","subj":"T3","obj":"NCBItxid:5052"},{"id":"A4","pred":"db_id","subj":"T4","obj":"NCBItxid:81076"},{"id":"A5","pred":"db_id","subj":"T5","obj":"NCBItxid:5062"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"},{"subj":"T5","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos6-CLO","denotations":[{"id":"T1","span":{"begin":997,"end":1001},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T2","span":{"begin":1244,"end":1252},"obj":"http://purl.obolibrary.org/obo/CLO_0001658"},{"id":"T3","span":{"begin":1380,"end":1384},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4","span":{"begin":1429,"end":1433},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-CLO"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-CLO"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-CLO"},{"subj":"T4","pred":"source","obj":"GlyCosmos6-CLO"}]},{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":808,"end":813},"obj":"FMAID:50596"},{"id":"_T2","span":{"begin":808,"end":813},"obj":"FMAID:146304"},{"id":"_T3","span":{"begin":997,"end":1016},"obj":"FMAID:200882"},{"id":"_T4","span":{"begin":997,"end":1016},"obj":"FMAID:86454"},{"id":"_T5","span":{"begin":1277,"end":1287},"obj":"FMAID:166112"},{"id":"_T6","span":{"begin":1280,"end":1287},"obj":"FMAID:67257"},{"id":"_T7","span":{"begin":1280,"end":1287},"obj":"FMAID:165447"},{"id":"_T8","span":{"begin":1304,"end":1310},"obj":"FMAID:178661"},{"id":"_T9","span":{"begin":1352,"end":1368},"obj":"FMAID:82742"},{"id":"_T10","span":{"begin":1352,"end":1368},"obj":"FMAID:196731"},{"id":"_T11","span":{"begin":1374,"end":1379},"obj":"FMAID:214748"},{"id":"_T12","span":{"begin":1423,"end":1428},"obj":"FMAID:214748"},{"id":"_T13","span":{"begin":1472,"end":1488},"obj":"FMAID:82742"},{"id":"_T14","span":{"begin":1472,"end":1488},"obj":"FMAID:196731"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T7","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T8","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T9","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T10","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T11","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T12","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T13","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T14","pred":"source","obj":"GlycoBiology-FMA"}]},{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":221,"end":227},"obj":"http://www.uniprot.org/uniprot/Q92988"},{"id":"T2","span":{"begin":1077,"end":1083},"obj":"http://www.uniprot.org/uniprot/Q92988"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-human"},{"subj":"T2","pred":"source","obj":"uniprot-human"}]},{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":27,"end":31},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T2","span":{"begin":27,"end":31},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T3","span":{"begin":51,"end":62},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/454240"},{"id":"T4","span":{"begin":51,"end":62},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/41750"},{"id":"T5","span":{"begin":51,"end":62},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/40382"},{"id":"T6","span":{"begin":51,"end":72},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/487661"},{"id":"T7","span":{"begin":221,"end":225},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T8","span":{"begin":221,"end":225},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T9","span":{"begin":286,"end":292},"obj":"http://purl.bioontology.org/ontology/STY/T004"},{"id":"T10","span":{"begin":293,"end":304},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/454240"},{"id":"T11","span":{"begin":293,"end":304},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/41750"},{"id":"T12","span":{"begin":293,"end":304},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/40382"},{"id":"T13","span":{"begin":293,"end":314},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/487661"},{"id":"T14","span":{"begin":528,"end":539},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/454240"},{"id":"T15","span":{"begin":528,"end":539},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/40382"},{"id":"T16","span":{"begin":528,"end":539},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/41750"},{"id":"T17","span":{"begin":580,"end":591},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/41750"},{"id":"T18","span":{"begin":580,"end":591},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/40382"},{"id":"T19","span":{"begin":580,"end":591},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/454240"},{"id":"T20","span":{"begin":592,"end":598},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/4527"},{"id":"T21","span":{"begin":1077,"end":1081},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T22","span":{"begin":1077,"end":1081},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T2","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T3","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T4","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T5","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T6","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T7","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T8","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T9","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T10","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T11","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T12","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T13","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T14","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T15","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T16","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T17","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T18","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T19","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T20","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T21","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T22","pred":"source","obj":"GlycoBiology-NCBITAXON"}]},{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":27,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0008810"},{"id":"T2","span":{"begin":27,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0031217"},{"id":"T3","span":{"begin":27,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0052859"},{"id":"T4","span":{"begin":242,"end":245},"obj":"http://purl.obolibrary.org/obo/GO_0033946"},{"id":"T5","span":{"begin":382,"end":385},"obj":"http://purl.obolibrary.org/obo/GO_0033946"},{"id":"T6","span":{"begin":484,"end":487},"obj":"http://purl.obolibrary.org/obo/GO_0033946"},{"id":"T7","span":{"begin":757,"end":760},"obj":"http://purl.obolibrary.org/obo/GO_0033946"},{"id":"T8","span":{"begin":1105,"end":1108},"obj":"http://purl.obolibrary.org/obo/GO_0033946"},{"id":"T9","span":{"begin":1445,"end":1448},"obj":"http://purl.obolibrary.org/obo/GO_0033946"},{"id":"T10","span":{"begin":697,"end":705},"obj":"http://purl.obolibrary.org/obo/GO_0015297"},{"id":"T11","span":{"begin":1140,"end":1149},"obj":"http://purl.obolibrary.org/obo/GO_0051235"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-BP"},{"subj":"T2","pred":"source","obj":"GO-BP"},{"subj":"T3","pred":"source","obj":"GO-BP"},{"subj":"T4","pred":"source","obj":"GO-BP"},{"subj":"T5","pred":"source","obj":"GO-BP"},{"subj":"T6","pred":"source","obj":"GO-BP"},{"subj":"T7","pred":"source","obj":"GO-BP"},{"subj":"T8","pred":"source","obj":"GO-BP"},{"subj":"T9","pred":"source","obj":"GO-BP"},{"subj":"T10","pred":"source","obj":"GO-BP"},{"subj":"T11","pred":"source","obj":"GO-BP"}]},{"project":"GO-MF","denotations":[{"id":"T1","span":{"begin":428,"end":435},"obj":"http://purl.obolibrary.org/obo/GO_0009055"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-MF"}]},{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":997,"end":1001},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T2","span":{"begin":1380,"end":1384},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3","span":{"begin":1429,"end":1433},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4","span":{"begin":997,"end":1006},"obj":"http://purl.obolibrary.org/obo/GO_0005618"},{"id":"T5","span":{"begin":1380,"end":1390},"obj":"http://purl.obolibrary.org/obo/GO_0005618"},{"id":"T6","span":{"begin":1429,"end":1439},"obj":"http://purl.obolibrary.org/obo/GO_0005618"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-CC"},{"subj":"T2","pred":"source","obj":"GO-CC"},{"subj":"T3","pred":"source","obj":"GO-CC"},{"subj":"T4","pred":"source","obj":"GO-CC"},{"subj":"T5","pred":"source","obj":"GO-CC"},{"subj":"T6","pred":"source","obj":"GO-CC"}]},{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":2,"end":12},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":195,"end":205},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"PD-GlycoEpitope-B_T3","span":{"begin":454,"end":464},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"PD-GlycoEpitope-B_T4","span":{"begin":939,"end":950},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"PD-GlycoEpitope-B_T5","span":{"begin":1051,"end":1061},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"PD-GlycoEpitope-B_T6","span":{"begin":1341,"end":1351},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"PD-GlycoEpitope-B_T7","span":{"begin":1461,"end":1471},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"},{"id":"PD-GlycoEpitope-B_T8","span":{"begin":1574,"end":1585},"obj":"http://www.glycoepitope.jp/epitopes/EP0511"}],"attributes":[{"subj":"PD-GlycoEpitope-B_T1","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T2","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T3","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T4","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T5","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T6","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T7","pred":"source","obj":"GlycoBiology-Epitope"},{"subj":"PD-GlycoEpitope-B_T8","pred":"source","obj":"GlycoBiology-Epitope"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"GlyCosmos6-UBERON","color":"#9a93ec","default":true},{"id":"Glycan-Motif","color":"#a5ec93"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ec93bf"},{"id":"Glycosmos6-GlycoEpitope","color":"#93d9ec"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#ece593"},{"id":"sentences","color":"#cb93ec"},{"id":"NCBITAXON","color":"#93ecb1"},{"id":"GlyCosmos6-CLO","color":"#ec9793"},{"id":"GlycoBiology-FMA","color":"#93a9ec"},{"id":"uniprot-human","color":"#c3ec93"},{"id":"GlycoBiology-NCBITAXON","color":"#ec93dd"},{"id":"GO-BP","color":"#93ece1"},{"id":"GO-MF","color":"#ecc793"},{"id":"GO-CC","color":"#ad93ec"},{"id":"GlycoBiology-Epitope","color":"#93ec93"}]}]}}