PubMed:9884411
Annnotations
Glycan-Motif
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 2-12 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T2 | 195-205 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T3 | 454-464 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T4 | 939-950 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucans |
T5 | 1051-1061 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T6 | 1341-1351 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T7 | 1461-1471 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T8 | 1574-1585 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucans |
GlyCosmos6-Glycan-Motif-Image
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 2-12 | Glycan_Motif | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
T2 | 195-205 | Glycan_Motif | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
T3 | 454-464 | Glycan_Motif | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
T4 | 939-950 | Glycan_Motif | denotes | xyloglucans | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
T5 | 1051-1061 | Glycan_Motif | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
T6 | 1341-1351 | Glycan_Motif | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
T7 | 1461-1471 | Glycan_Motif | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
T8 | 1574-1585 | Glycan_Motif | denotes | xyloglucans | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC |
Glycosmos6-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 2-12 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
T2 | 195-205 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
T3 | 454-464 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
T4 | 1051-1061 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
T5 | 1341-1351 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
T6 | 1461-1471 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
GlyCosmos6-Glycan-Motif-Structure
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 2-12 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T2 | 195-205 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T3 | 454-464 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T4 | 939-950 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucans |
T5 | 1051-1061 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T6 | 1341-1351 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T7 | 1461-1471 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucan |
T8 | 1574-1585 | https://glytoucan.org/Structures/Glycans/G25326UC | denotes | xyloglucans |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
TextSentencer_T1 | 0-163 | Sentence | denotes | A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
TextSentencer_T2 | 164-346 | Sentence | denotes | A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
TextSentencer_T3 | 347-465 | Sentence | denotes | The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
TextSentencer_T4 | 466-627 | Sentence | denotes | The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
TextSentencer_T5 | 628-740 | Sentence | denotes | The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
TextSentencer_T6 | 741-895 | Sentence | denotes | The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
TextSentencer_T7 | 896-1104 | Sentence | denotes | The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
TextSentencer_T8 | 1105-1180 | Sentence | denotes | XEG hydrolyzes its substrates with retention of the anomeric configuration. |
TextSentencer_T9 | 1181-1288 | Sentence | denotes | The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
TextSentencer_T10 | 1289-1391 | Sentence | denotes | The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
TextSentencer_T11 | 1392-1586 | Sentence | denotes | It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
T1 | 0-163 | Sentence | denotes | A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
T2 | 164-346 | Sentence | denotes | A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
T3 | 347-465 | Sentence | denotes | The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
T4 | 466-627 | Sentence | denotes | The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
T5 | 628-740 | Sentence | denotes | The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
T6 | 741-895 | Sentence | denotes | The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
T7 | 896-1104 | Sentence | denotes | The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
T8 | 1105-1180 | Sentence | denotes | XEG hydrolyzes its substrates with retention of the anomeric configuration. |
T9 | 1181-1288 | Sentence | denotes | The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
T10 | 1289-1391 | Sentence | denotes | The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
T11 | 1392-1586 | Sentence | denotes | It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
T1 | 0-163 | Sentence | denotes | A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
T2 | 164-346 | Sentence | denotes | A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
T3 | 347-465 | Sentence | denotes | The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
T4 | 466-627 | Sentence | denotes | The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
T5 | 628-740 | Sentence | denotes | The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
T6 | 741-895 | Sentence | denotes | The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
T7 | 896-1104 | Sentence | denotes | The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
T8 | 1105-1180 | Sentence | denotes | XEG hydrolyzes its substrates with retention of the anomeric configuration. |
T9 | 1181-1288 | Sentence | denotes | The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
T10 | 1289-1391 | Sentence | denotes | The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
T11 | 1392-1586 | Sentence | denotes | It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
GlycoBiology-FMA
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
_T1 | 808-813 | FMAID:50596 | denotes | point |
_T2 | 808-813 | FMAID:146304 | denotes | point |
_T3 | 997-1016 | FMAID:200882 | denotes | cell wall component |
_T4 | 997-1016 | FMAID:86454 | denotes | cell wall component |
_T5 | 1277-1287 | FMAID:166112 | denotes | mg protein |
_T6 | 1280-1287 | FMAID:67257 | denotes | protein |
_T7 | 1280-1287 | FMAID:165447 | denotes | protein |
_T8 | 1304-1310 | FMAID:178661 | denotes | tested |
_T9 | 1352-1368 | FMAID:82742 | denotes | oligosaccharides |
_T10 | 1352-1368 | FMAID:196731 | denotes | oligosaccharides |
_T11 | 1374-1379 | FMAID:214748 | denotes | plant |
_T12 | 1423-1428 | FMAID:214748 | denotes | plant |
_T13 | 1472-1488 | FMAID:82742 | denotes | oligosaccharides |
_T14 | 1472-1488 | FMAID:196731 | denotes | oligosaccharides |
uniprot-human
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 221-227 | http://www.uniprot.org/uniprot/Q92988 | denotes | beta-1 |
T2 | 1077-1083 | http://www.uniprot.org/uniprot/Q92988 | denotes | beta-1 |
GlycoBiology-NCBITAXON
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 27-31 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
T2 | 27-31 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
T3 | 51-62 | http://purl.bioontology.org/ontology/NCBITAXON/454240 | denotes | Aspergillus |
T4 | 51-62 | http://purl.bioontology.org/ontology/NCBITAXON/41750 | denotes | Aspergillus |
T5 | 51-62 | http://purl.bioontology.org/ontology/NCBITAXON/40382 | denotes | Aspergillus |
T6 | 51-72 | http://purl.bioontology.org/ontology/NCBITAXON/487661 | denotes | Aspergillus aculeatus |
T7 | 221-225 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
T8 | 221-225 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
T9 | 286-292 | http://purl.bioontology.org/ontology/STY/T004 | denotes | fungus |
T10 | 293-304 | http://purl.bioontology.org/ontology/NCBITAXON/454240 | denotes | Aspergillus |
T11 | 293-304 | http://purl.bioontology.org/ontology/NCBITAXON/41750 | denotes | Aspergillus |
T12 | 293-304 | http://purl.bioontology.org/ontology/NCBITAXON/40382 | denotes | Aspergillus |
T13 | 293-314 | http://purl.bioontology.org/ontology/NCBITAXON/487661 | denotes | Aspergillus aculeatus |
T14 | 528-539 | http://purl.bioontology.org/ontology/NCBITAXON/454240 | denotes | Aspergillus |
T15 | 528-539 | http://purl.bioontology.org/ontology/NCBITAXON/40382 | denotes | Aspergillus |
T16 | 528-539 | http://purl.bioontology.org/ontology/NCBITAXON/41750 | denotes | Aspergillus |
T17 | 580-591 | http://purl.bioontology.org/ontology/NCBITAXON/41750 | denotes | Aspergillus |
T18 | 580-591 | http://purl.bioontology.org/ontology/NCBITAXON/40382 | denotes | Aspergillus |
T19 | 580-591 | http://purl.bioontology.org/ontology/NCBITAXON/454240 | denotes | Aspergillus |
T20 | 592-598 | http://purl.bioontology.org/ontology/NCBITAXON/4527 | denotes | oryzae |
T21 | 1077-1081 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
T22 | 1077-1081 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 27-45 | http://purl.obolibrary.org/obo/GO_0008810 | denotes | beta-1,4-glucanase |
T2 | 27-45 | http://purl.obolibrary.org/obo/GO_0031217 | denotes | beta-1,4-glucanase |
T3 | 27-45 | http://purl.obolibrary.org/obo/GO_0052859 | denotes | beta-1,4-glucanase |
T4 | 242-245 | http://purl.obolibrary.org/obo/GO_0033946 | denotes | XEG |
T5 | 382-385 | http://purl.obolibrary.org/obo/GO_0033946 | denotes | XEG |
T6 | 484-487 | http://purl.obolibrary.org/obo/GO_0033946 | denotes | XEG |
T7 | 757-760 | http://purl.obolibrary.org/obo/GO_0033946 | denotes | XEG |
T8 | 1105-1108 | http://purl.obolibrary.org/obo/GO_0033946 | denotes | XEG |
T9 | 1445-1448 | http://purl.obolibrary.org/obo/GO_0033946 | denotes | XEG |
T10 | 697-705 | http://purl.obolibrary.org/obo/GO_0015297 | denotes | exchange |
T11 | 1140-1149 | http://purl.obolibrary.org/obo/GO_0051235 | denotes | retention |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 428-435 | http://purl.obolibrary.org/obo/GO_0009055 | denotes | azurine |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 997-1001 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
T2 | 1380-1384 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
T3 | 1429-1433 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
T4 | 997-1006 | http://purl.obolibrary.org/obo/GO_0005618 | denotes | cell wall |
T5 | 1380-1390 | http://purl.obolibrary.org/obo/GO_0005618 | denotes | cell walls |
T6 | 1429-1439 | http://purl.obolibrary.org/obo/GO_0005618 | denotes | cell walls |
GlycoBiology-Epitope
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
PD-GlycoEpitope-B_T1 | 2-12 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
PD-GlycoEpitope-B_T2 | 195-205 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
PD-GlycoEpitope-B_T3 | 454-464 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
PD-GlycoEpitope-B_T4 | 939-950 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucans |
PD-GlycoEpitope-B_T5 | 1051-1061 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
PD-GlycoEpitope-B_T6 | 1341-1351 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
PD-GlycoEpitope-B_T7 | 1461-1471 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucan |
PD-GlycoEpitope-B_T8 | 1574-1585 | http://www.glycoepitope.jp/epitopes/EP0511 | denotes | xyloglucans |
Glycan-GlyCosmos
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 2-12 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T2 | 195-205 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T3 | 454-464 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T4 | 1051-1061 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T5 | 1341-1351 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T6 | 1461-1471 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
GlyCosmos15-NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 51-72 | OrganismTaxon | denotes | Aspergillus aculeatus | 5053 |
T2 | 293-314 | OrganismTaxon | denotes | Aspergillus aculeatus | 5053 |
T3 | 528-539 | OrganismTaxon | denotes | Aspergillus | 5052 |
T4 | 580-598 | OrganismTaxon | denotes | Aspergillus oryzae | 5062 |
GlyCosmos15-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 1002-1006 | Body_part | denotes | wall | http://purl.obolibrary.org/obo/UBERON_0000060 |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
TextSentencer_T1 | 0-163 | Sentence | denotes | A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
TextSentencer_T2 | 164-346 | Sentence | denotes | A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
TextSentencer_T3 | 347-465 | Sentence | denotes | The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
TextSentencer_T4 | 466-627 | Sentence | denotes | The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
TextSentencer_T5 | 628-740 | Sentence | denotes | The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
TextSentencer_T6 | 741-895 | Sentence | denotes | The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
TextSentencer_T7 | 896-1104 | Sentence | denotes | The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
TextSentencer_T8 | 1105-1180 | Sentence | denotes | XEG hydrolyzes its substrates with retention of the anomeric configuration. |
TextSentencer_T9 | 1181-1288 | Sentence | denotes | The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
TextSentencer_T10 | 1289-1391 | Sentence | denotes | The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
TextSentencer_T11 | 1392-1586 | Sentence | denotes | It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
T1 | 0-163 | Sentence | denotes | A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
T2 | 164-346 | Sentence | denotes | A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
T3 | 347-465 | Sentence | denotes | The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
T4 | 466-627 | Sentence | denotes | The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
T5 | 628-740 | Sentence | denotes | The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
T6 | 741-895 | Sentence | denotes | The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
T7 | 896-1104 | Sentence | denotes | The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
T8 | 1105-1180 | Sentence | denotes | XEG hydrolyzes its substrates with retention of the anomeric configuration. |
T9 | 1181-1288 | Sentence | denotes | The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
T10 | 1289-1391 | Sentence | denotes | The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
T11 | 1392-1586 | Sentence | denotes | It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
T1 | 0-163 | Sentence | denotes | A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
T2 | 164-346 | Sentence | denotes | A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
T3 | 347-465 | Sentence | denotes | The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
T4 | 466-627 | Sentence | denotes | The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
T5 | 628-740 | Sentence | denotes | The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
T6 | 741-895 | Sentence | denotes | The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
T7 | 896-1104 | Sentence | denotes | The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
T8 | 1105-1180 | Sentence | denotes | XEG hydrolyzes its substrates with retention of the anomeric configuration. |
T9 | 1181-1288 | Sentence | denotes | The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
T10 | 1289-1391 | Sentence | denotes | The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
T11 | 1392-1586 | Sentence | denotes | It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
GlyCosmos15-Sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-163 | Sentence | denotes | A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme. |
T2 | 164-346 | Sentence | denotes | A full-length c-DNA encoding a xyloglucan-specific endo -beta-1, 4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. |
T3 | 347-465 | Sentence | denotes | The colonies expressing functional XEG were identified on agar plates containing azurine-dyed cross-linked xyloglucan. |
T4 | 466-627 | Sentence | denotes | The cDNA encoding XEG was isolated, sequenced, cloned into an Aspergillus expression vector, and transformed into Aspergillus oryzae for heterologous expression. |
T5 | 628-740 | Sentence | denotes | The recombinant enzyme was purified to apparent homogeneity by anion-exchange and gel permeation chromatography. |
T6 | 741-895 | Sentence | denotes | The recombinant XEG has a molecular mass of 23,600, an isoelectric point of 3.4, and is optimally stable at a pH of 3.4 and temperature below 30 degreesC. |
T7 | 896-1104 | Sentence | denotes | The enzyme hydrolyzes structurally diverse xyloglucans from various sources, but hydrolyzes no other cell wall component and can therefore be considered a xyloglucan-specific endo -beta-1, 4-glucanohydrolase. |
T8 | 1105-1180 | Sentence | denotes | XEG hydrolyzes its substrates with retention of the anomeric configuration. |
T9 | 1181-1288 | Sentence | denotes | The Kmof the recombinant enzyme is 3.6 mg/ml, and its specific activity is 260 micromol/min per mg protein. |
T10 | 1289-1391 | Sentence | denotes | The enzyme was tested for its ability to solubilize xyloglucan oligosaccharides from plant cell walls. |
T11 | 1392-1586 | Sentence | denotes | It was shown that treatment of plant cell walls with XEG yields only xyloglucan oligosaccharides, indicating that this enzyme can be a powerful tool in the structural elucidation of xyloglucans. |
GlyCosmos15-Glycan
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 2-12 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T2 | 195-205 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T3 | 454-464 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T4 | 1051-1061 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T5 | 1341-1351 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
T6 | 1461-1471 | Glycan | denotes | xyloglucan | https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC |
GlyCosmos15-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
---|---|---|---|---|---|
T1 | 2-12 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T2 | 195-205 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T3 | 454-464 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T4 | 1051-1061 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T5 | 1341-1351 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T6 | 1461-1471 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 51-72 | OrganismTaxon | denotes | Aspergillus aculeatus | 5053 |
T2 | 293-314 | OrganismTaxon | denotes | Aspergillus aculeatus | 5053 |
T3 | 528-539 | OrganismTaxon | denotes | Aspergillus | 5052 |
T4 | 580-598 | OrganismTaxon | denotes | Aspergillus oryzae | 5062 |
GlyCosmos-GlycoEpitope
Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
---|---|---|---|---|---|
T1 | 2-12 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T2 | 195-205 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T3 | 454-464 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T4 | 1051-1061 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T5 | 1341-1351 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
T6 | 1461-1471 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | xyloglucan | http://www.glycoepitope.jp/epitopes/EP0511 |
Anatomy-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 1002-1006 | Body_part | denotes | wall | http://purl.obolibrary.org/obo/UBERON_0000060 |