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PubMed:9774702 JSONTXT

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mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 1310-1318 Disease denotes diabetes http://purl.obolibrary.org/obo/MONDO_0005015
T2 1323-1330 Disease denotes obesity http://purl.obolibrary.org/obo/MONDO_0011122
T3 2202-2205 Disease denotes PPA http://purl.obolibrary.org/obo/MONDO_0019806

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 1323-1330 Phenotype denotes obesity HP:0001513

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 1354-1361 Glycan denotes amylose https://api.glycosmos.org/wurcs2image/latest/png/binary/G05740LL
T2 2152-2159 Glycan denotes amylose https://api.glycosmos.org/wurcs2image/latest/png/binary/G05740LL

GlyCosmos15-HP

Id Subject Object Predicate Lexical cue hp_id
T1 1323-1330 Phenotype denotes obesity HP:0001513

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 1310-1318 Disease denotes diabetes http://purl.obolibrary.org/obo/MONDO_0005015
T2 1323-1330 Disease denotes obesity http://purl.obolibrary.org/obo/MONDO_0011122
T3 2202-2205 Disease denotes PPA http://purl.obolibrary.org/obo/MONDO_0019806

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 4-9 OrganismTaxon denotes human 9606
T2 183-188 OrganismTaxon denotes human 9606
T3 652-657 OrganismTaxon denotes human 9606
T4 1181-1189 OrganismTaxon denotes chelator 263156
T5 1953-1958 OrganismTaxon denotes human 9606

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-114 Sentence denotes The human pancreatic alpha-amylase isoforms: isolation, structural studies and kinetics of inhibition by acarbose.
T2 115-233 Sentence denotes A rapid method is proposed for isolating the two main components of human pancreatic alpha-amylase (HPA I and HPA II).
T3 234-385 Sentence denotes The isoelectric point of HPA I (7.2), the main component, was determined using an isoelectrofocusing method and found to differ from that of HPA II (6.
T4 386-389 Sentence denotes 6).
T5 390-686 Sentence denotes The molecular mass of HPA I (55862+/-5 Da) and that of HPA II (55786+/-5 Da) were determined by performing mass spectrometry and found to be quite similar to that of the protein moiety calculated from the amino acid sequence (55788 Da), which indicates that the human amylase is not glycosylated.
T6 687-785 Sentence denotes The structure of both HPA I and HPA II was further investigated by performing limited proteolysis.
T7 786-1030 Sentence denotes Two fragments with an apparent molecular mass of 41 kDa and 14 kDa were obtained by digesting the isoforms with proteinase K and subtilisin, whereas digestion with papain yielded two cleaved fragments with molecular masses of 38 kDa and 17 kDa.
T8 1031-1224 Sentence denotes Proteinase K and subtilisin susceptible bonds are located in the L8 loop (A domain), while the papain cut which occurs in the presence of the calcium chelator EDTA is in the L3 loop (B domain).
T9 1225-1372 Sentence denotes The kinetics of the inhibition of HPA I and HPA II by acarbose, a drug used to treat diabetes and obesity, were studied using an amylose substrate.
T10 1373-1532 Sentence denotes The Lineweaver-Burk primary plots of HPA I and HPA II, which did not differ significantly, indicated that the inhibition was of the mixed non-competitive type.
T11 1533-1575 Sentence denotes The secondary plots gave parabolic curves.
T12 1576-1656 Sentence denotes All in all, these data provide evidence that two acarbose molecules bind to HPA.
T13 1657-1846 Sentence denotes In conclusion, apart from the pI, no significant differences were observed between HPA I and HPA II as regards either their molecular mass and limited proteolysis or their kinetic behavior.
T14 1847-2121 Sentence denotes As was to be expected in view of the high degree of structural identity previously found to exist between human and porcine pancreatic amylases, the present data show that the inhibitory effects of acarbose on the kinetic behavior of these two amylases are quite comparable.
T15 2122-2294 Sentence denotes In particular, the process of amylose hydrolysis catalyzed by HPA as well as by PPA in both cases requires two carbohydrate binding sites in addition to the catalytic site.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 1354-1361 Glycan denotes amylose https://api.glycosmos.org/wurcs2image/latest/png/binary/G05740LL
T2 2152-2159 Glycan denotes amylose https://api.glycosmos.org/wurcs2image/latest/png/binary/G05740LL

GlyCosmos15-Lectin-Jamboree

Id Subject Object Predicate Lexical cue glycosmos_id
T1 215-218 Lectin denotes HPA GL_005054
T2 225-228 Lectin denotes HPA GL_005054
T3 259-262 Lectin denotes HPA GL_005054
T4 375-378 Lectin denotes HPA GL_005054
T5 412-415 Lectin denotes HPA GL_005054
T6 445-448 Lectin denotes HPA GL_005054
T7 709-712 Lectin denotes HPA GL_005054
T8 719-722 Lectin denotes HPA GL_005054
T9 1259-1262 Lectin denotes HPA GL_005054
T10 1269-1272 Lectin denotes HPA GL_005054
T11 1410-1413 Lectin denotes HPA GL_005054
T12 1420-1423 Lectin denotes HPA GL_005054
T13 1652-1655 Lectin denotes HPA GL_005054
T14 1740-1743 Lectin denotes HPA GL_005054
T15 1750-1753 Lectin denotes HPA GL_005054
T16 2184-2187 Lectin denotes HPA GL_005054

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 4-9 OrganismTaxon denotes human 9606
T2 183-188 OrganismTaxon denotes human 9606
T3 652-657 OrganismTaxon denotes human 9606
T4 1181-1189 OrganismTaxon denotes chelator 263156
T5 1953-1958 OrganismTaxon denotes human 9606