PubMed:9530962 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/9530962","sourcedb":"PubMed","sourceid":"9530962","text":"HPLC method for the determination of Fuc to Asn-linked GlcNAc fucosyltransferases.\nMammalian cells often contain an enzyme which transfers fucose onto the reducing terminal GlcNAc (GlcNAc-1) of N-glycans with an alpha1,6-linkage. In plants, on the other hand, the fucose is transferred to GlcNAc-1 with an alpha1,3-inkage. Insect cells can exhibit both enzymatic activities. Hitherto, the activity of these fucosyltransferases has been determined by the incorporation of radioactively labelled fucose into an acceptor glycopeptide. This assay, however, cannot discriminate these two activities. Here we report on the use of dansylated glycoasparagine for the specific determination of 1,3- and 1,6-fucosyltransferases. 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