PubMed:9419430 JSONTXT

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":24,"end":53},"obj":"protein"},{"id":"T2","span":{"begin":75,"end":91},"obj":"protein"},{"id":"T3","span":{"begin":95,"end":104},"obj":"protein"},{"id":"T4","span":{"begin":108,"end":123},"obj":"protein"},{"id":"T5","span":{"begin":125,"end":128},"obj":"protein"},{"id":"T6","span":{"begin":153,"end":169},"obj":"protein"},{"id":"T7","span":{"begin":191,"end":200},"obj":"protein"},{"id":"T8","span":{"begin":221,"end":225},"obj":"protein"},{"id":"T9","span":{"begin":301,"end":310},"obj":"protein"},{"id":"T10","span":{"begin":324,"end":336},"obj":"protein"},{"id":"T11","span":{"begin":362,"end":366},"obj":"protein"},{"id":"T12","span":{"begin":409,"end":416},"obj":"protein"},{"id":"T13","span":{"begin":430,"end":439},"obj":"protein"},{"id":"T14","span":{"begin":471,"end":480},"obj":"protein"},{"id":"T15","span":{"begin":507,"end":511},"obj":"protein"},{"id":"T16","span":{"begin":525,"end":532},"obj":"cell_type"},{"id":"T17","span":{"begin":548,"end":557},"obj":"protein"},{"id":"T18","span":{"begin":585,"end":594},"obj":"protein"},{"id":"T19","span":{"begin":605,"end":609},"obj":"protein"},{"id":"T20","span":{"begin":644,"end":658},"obj":"protein"},{"id":"T21","span":{"begin":660,"end":683},"obj":"protein"},{"id":"T22","span":{"begin":764,"end":776},"obj":"protein"},{"id":"T23","span":{"begin":780,"end":785},"obj":"protein"}],"text":"Agonistic activity of a CD40-specific single-chain Fv constructed from the variable regions of mAb G28-5.\nA single-chain Fv (sFv) was expressed from the variable regions of the CD40-specific mAb G28-5. The molecule bound CD40 with a high affinity (2.2 nM) and was a monomer in solution. Surprisingly, G28-5 sFv was a potent CD40 agonist that rapidly crosslinked CD40 on the cell surface but did not crosslink CD40-Ig in solution. G28-5 sFv was a more potent agonist than G28-5 IgG and was able to stimulate CD40 responses by B cells and monocytes. G28-5 IgG partially blocked, whereas G28-5 sFv augmented CD40 responses during stimulation with natural ligand (gp39-CD8 fusion protein). These results indicate that the functional activity of ligands built from the binding site of G28-5 is highly dependent upon the size and physical properties of the molecule both in solution and on the cell surfaces."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":221,"end":225},"obj":"NP"},{"id":"C2","span":{"begin":277,"end":285},"obj":"NP"},{"id":"C3","span":{"begin":301,"end":310},"obj":"NP"},{"id":"C4","span":{"begin":315,"end":336},"obj":"NP"},{"id":"C5","span":{"begin":337,"end":341},"obj":"NP"},{"id":"C6","span":{"begin":362,"end":366},"obj":"NP"},{"id":"C7","span":{"begin":370,"end":386},"obj":"NP"},{"id":"C8","span":{"begin":420,"end":428},"obj":"NP"},{"id":"C9","span":{"begin":430,"end":439},"obj":"NP"},{"id":"C10","span":{"begin":471,"end":480},"obj":"NP"},{"id":"C11","span":{"begin":507,"end":521},"obj":"NP"},{"id":"C12","span":{"begin":548,"end":557},"obj":"NP"},{"id":"C13","span":{"begin":585,"end":594},"obj":"NP"},{"id":"C14","span":{"begin":605,"end":619},"obj":"NP"},{"id":"C15","span":{"begin":868,"end":876},"obj":"NP"},{"id":"C16","span":{"begin":884,"end":901},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-relat","subj":"C5","obj":"C4"},{"id":"R2","pred":"coref-ident","subj":"C6","obj":"C1"},{"id":"R3","pred":"coref-ident","subj":"C8","obj":"C2"},{"id":"R4","pred":"coref-ident","subj":"C9","obj":"C3"},{"id":"R5","pred":"coref-ident","subj":"C12","obj":"C10"},{"id":"R6","pred":"coref-ident","subj":"C13","obj":"C9"},{"id":"R7","pred":"coref-ident","subj":"C14","obj":"C11"},{"id":"R8","pred":"coref-ident","subj":"C15","obj":"C8"},{"id":"R9","pred":"coref-ident","subj":"C16","obj":"C7"}],"text":"Agonistic activity of a CD40-specific single-chain Fv constructed from the variable regions of mAb G28-5.\nA single-chain Fv (sFv) was expressed from the variable regions of the CD40-specific mAb G28-5. The molecule bound CD40 with a high affinity (2.2 nM) and was a monomer in solution. Surprisingly, G28-5 sFv was a potent CD40 agonist that rapidly crosslinked CD40 on the cell surface but did not crosslink CD40-Ig in solution. G28-5 sFv was a more potent agonist than G28-5 IgG and was able to stimulate CD40 responses by B cells and monocytes. G28-5 IgG partially blocked, whereas G28-5 sFv augmented CD40 responses during stimulation with natural ligand (gp39-CD8 fusion protein). These results indicate that the functional activity of ligands built from the binding site of G28-5 is highly dependent upon the size and physical properties of the molecule both in solution and on the cell surfaces."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":105},"obj":"Sentence"},{"id":"S2","span":{"begin":106,"end":201},"obj":"Sentence"},{"id":"S3","span":{"begin":202,"end":286},"obj":"Sentence"},{"id":"S4","span":{"begin":287,"end":429},"obj":"Sentence"},{"id":"S5","span":{"begin":430,"end":547},"obj":"Sentence"},{"id":"S6","span":{"begin":548,"end":685},"obj":"Sentence"},{"id":"S7","span":{"begin":686,"end":902},"obj":"Sentence"}],"text":"Agonistic activity of a CD40-specific single-chain Fv constructed from the variable regions of mAb G28-5.\nA single-chain Fv (sFv) was expressed from the variable regions of the CD40-specific mAb G28-5. The molecule bound CD40 with a high affinity (2.2 nM) and was a monomer in solution. Surprisingly, G28-5 sFv was a potent CD40 agonist that rapidly crosslinked CD40 on the cell surface but did not crosslink CD40-Ig in solution. G28-5 sFv was a more potent agonist than G28-5 IgG and was able to stimulate CD40 responses by B cells and monocytes. G28-5 IgG partially blocked, whereas G28-5 sFv augmented CD40 responses during stimulation with natural ligand (gp39-CD8 fusion protein). These results indicate that the functional activity of ligands built from the binding site of G28-5 is highly dependent upon the size and physical properties of the molecule both in solution and on the cell surfaces."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":18},"obj":"other_name"},{"id":"T2","span":{"begin":24,"end":53},"obj":"protein_molecule"},{"id":"T3","span":{"begin":75,"end":91},"obj":"protein_domain_or_region"},{"id":"T4","span":{"begin":95,"end":104},"obj":"protein_molecule"},{"id":"T5","span":{"begin":108,"end":123},"obj":"protein_molecule"},{"id":"T6","span":{"begin":125,"end":128},"obj":"protein_molecule"},{"id":"T7","span":{"begin":153,"end":169},"obj":"protein_domain_or_region"},{"id":"T8","span":{"begin":191,"end":200},"obj":"protein_molecule"},{"id":"T9","span":{"begin":221,"end":225},"obj":"protein_molecule"},{"id":"T10","span":{"begin":301,"end":310},"obj":"protein_molecule"},{"id":"T11","span":{"begin":324,"end":328},"obj":"protein_molecule"},{"id":"T12","span":{"begin":362,"end":366},"obj":"protein_molecule"},{"id":"T13","span":{"begin":374,"end":386},"obj":"cell_component"},{"id":"T14","span":{"begin":409,"end":413},"obj":"protein_molecule"},{"id":"T15","span":{"begin":430,"end":439},"obj":"protein_molecule"},{"id":"T16","span":{"begin":471,"end":480},"obj":"protein_molecule"},{"id":"T17","span":{"begin":507,"end":511},"obj":"protein_molecule"},{"id":"T18","span":{"begin":525,"end":532},"obj":"cell_type"},{"id":"T19","span":{"begin":548,"end":557},"obj":"protein_molecule"},{"id":"T20","span":{"begin":585,"end":594},"obj":"protein_molecule"},{"id":"T21","span":{"begin":605,"end":609},"obj":"protein_molecule"},{"id":"T22","span":{"begin":644,"end":658},"obj":"protein_family_or_group"},{"id":"T23","span":{"begin":660,"end":683},"obj":"protein_molecule"},{"id":"T24","span":{"begin":718,"end":737},"obj":"other_name"},{"id":"T25","span":{"begin":764,"end":776},"obj":"protein_domain_or_region"},{"id":"T26","span":{"begin":780,"end":785},"obj":"protein_molecule"},{"id":"T27","span":{"begin":888,"end":901},"obj":"cell_component"}],"text":"Agonistic activity of a CD40-specific single-chain Fv constructed from the variable regions of mAb G28-5.\nA single-chain Fv (sFv) was expressed from the variable regions of the CD40-specific mAb G28-5. The molecule bound CD40 with a high affinity (2.2 nM) and was a monomer in solution. Surprisingly, G28-5 sFv was a potent CD40 agonist that rapidly crosslinked CD40 on the cell surface but did not crosslink CD40-Ig in solution. G28-5 sFv was a more potent agonist than G28-5 IgG and was able to stimulate CD40 responses by B cells and monocytes. G28-5 IgG partially blocked, whereas G28-5 sFv augmented CD40 responses during stimulation with natural ligand (gp39-CD8 fusion protein). These results indicate that the functional activity of ligands built from the binding site of G28-5 is highly dependent upon the size and physical properties of the molecule both in solution and on the cell surfaces."}