PubMed:9406848 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/9406848","sourcedb":"PubMed","sourceid":"9406848","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/9406848","text":"C/EBP activates the human corticotropin-releasing hormone gene promoter.\nThe purpose of these studies was to identify whether transcription factors, associated with cytokine signalling, affected promoter activity of the corticotropin releasing hormone (CRH) gene. Fragments of a 3.6 kb sequence of the human CRH gene promoter were amplified by PCR and ligated upstream of a CAT reporter. These constructs were transfected into a variety of cell lines, either alone or together, with transcription factor expression vectors. Basal activity of a 3070 bp CRH promoter fragment was only seen in neuronal and lymphoblastoid cell lines. Promoter activity was increased by the transcription factors C/EBPbeta (NF-IL6) and more strongly, by C/EBPdelta (NF-IL6beta). Increased CRH promoter activity following phorbol ester treatment was inhibited by a dominant negative NF-IL6 mutant, showing that the effects of phorbol ester were principally mediated by C/EBP. Moreover, the inverse changes in the expression of CRH in the hypothalamus and spleens of arthritic rats were paralleled by similar inverse changes in NF-IL6beta expression in these organs. These data show that some transcription factors associated with cytokine signalling can also activate the CRH promoter.","tracks":[{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":0,"end":5},"obj":"NP"},{"id":"C2","span":{"begin":16,"end":71},"obj":"NP"},{"id":"C3","span":{"begin":298,"end":325},"obj":"NP"},{"id":"C4","span":{"begin":947,"end":952},"obj":"NP"},{"id":"C5","span":{"begin":1012,"end":1058},"obj":"NP"},{"id":"C6","span":{"begin":1130,"end":1142},"obj":"NP"},{"id":"C7","span":{"begin":1246,"end":1262},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C3","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C4","obj":"C1"},{"id":"R3","pred":"coref-ident","subj":"C6","obj":"C5"},{"id":"R4","pred":"coref-ident","subj":"C7","obj":"C3"}],"attributes":[{"subj":"C1","pred":"source","obj":"genia-medco-coref"},{"subj":"C2","pred":"source","obj":"genia-medco-coref"},{"subj":"C3","pred":"source","obj":"genia-medco-coref"},{"subj":"C4","pred":"source","obj":"genia-medco-coref"},{"subj":"C5","pred":"source","obj":"genia-medco-coref"},{"subj":"C6","pred":"source","obj":"genia-medco-coref"},{"subj":"C7","pred":"source","obj":"genia-medco-coref"}]},{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":0,"end":5},"obj":"protein"},{"id":"T2","span":{"begin":20,"end":71},"obj":"DNA"},{"id":"T3","span":{"begin":220,"end":262},"obj":"DNA"},{"id":"T4","span":{"begin":302,"end":325},"obj":"DNA"},{"id":"T5","span":{"begin":374,"end":386},"obj":"DNA"},{"id":"T6","span":{"begin":483,"end":503},"obj":"protein"},{"id":"T7","span":{"begin":544,"end":573},"obj":"DNA"},{"id":"T8","span":{"begin":604,"end":629},"obj":"cell_line"},{"id":"T9","span":{"begin":670,"end":691},"obj":"protein"},{"id":"T10","span":{"begin":692,"end":701},"obj":"protein"},{"id":"T11","span":{"begin":703,"end":709},"obj":"protein"},{"id":"T12","span":{"begin":733,"end":743},"obj":"protein"},{"id":"T13","span":{"begin":745,"end":755},"obj":"protein"},{"id":"T14","span":{"begin":768,"end":771},"obj":"protein"},{"id":"T15","span":{"begin":861,"end":874},"obj":"protein"},{"id":"T16","span":{"begin":947,"end":952},"obj":"protein"},{"id":"T17","span":{"begin":1005,"end":1008},"obj":"protein"},{"id":"T18","span":{"begin":1105,"end":1115},"obj":"protein"},{"id":"T19","span":{"begin":1170,"end":1191},"obj":"protein"},{"id":"T20","span":{"begin":1208,"end":1216},"obj":"protein"},{"id":"T21","span":{"begin":1250,"end":1262},"obj":"DNA"}],"attributes":[{"subj":"T1","pred":"source","obj":"jnlpba-st-training"},{"subj":"T2","pred":"source","obj":"jnlpba-st-training"},{"subj":"T3","pred":"source","obj":"jnlpba-st-training"},{"subj":"T4","pred":"source","obj":"jnlpba-st-training"},{"subj":"T5","pred":"source","obj":"jnlpba-st-training"},{"subj":"T6","pred":"source","obj":"jnlpba-st-training"},{"subj":"T7","pred":"source","obj":"jnlpba-st-training"},{"subj":"T8","pred":"source","obj":"jnlpba-st-training"},{"subj":"T9","pred":"source","obj":"jnlpba-st-training"},{"subj":"T10","pred":"source","obj":"jnlpba-st-training"},{"subj":"T11","pred":"source","obj":"jnlpba-st-training"},{"subj":"T12","pred":"source","obj":"jnlpba-st-training"},{"subj":"T13","pred":"source","obj":"jnlpba-st-training"},{"subj":"T14","pred":"source","obj":"jnlpba-st-training"},{"subj":"T15","pred":"source","obj":"jnlpba-st-training"},{"subj":"T16","pred":"source","obj":"jnlpba-st-training"},{"subj":"T17","pred":"source","obj":"jnlpba-st-training"},{"subj":"T18","pred":"source","obj":"jnlpba-st-training"},{"subj":"T19","pred":"source","obj":"jnlpba-st-training"},{"subj":"T20","pred":"source","obj":"jnlpba-st-training"},{"subj":"T21","pred":"source","obj":"jnlpba-st-training"}]},{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":72},"obj":"Sentence"},{"id":"S2","span":{"begin":73,"end":263},"obj":"Sentence"},{"id":"S3","span":{"begin":264,"end":387},"obj":"Sentence"},{"id":"S4","span":{"begin":388,"end":523},"obj":"Sentence"},{"id":"S5","span":{"begin":524,"end":630},"obj":"Sentence"},{"id":"S6","span":{"begin":631,"end":757},"obj":"Sentence"},{"id":"S7","span":{"begin":758,"end":953},"obj":"Sentence"},{"id":"S8","span":{"begin":954,"end":1143},"obj":"Sentence"},{"id":"S9","span":{"begin":1144,"end":1263},"obj":"Sentence"}],"attributes":[{"subj":"S1","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S2","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S3","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S4","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S5","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S6","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S7","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S8","pred":"source","obj":"pubmed-sentences-benchmark"},{"subj":"S9","pred":"source","obj":"pubmed-sentences-benchmark"}]},{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":5},"obj":"protein_family_or_group"},{"id":"T2","span":{"begin":20,"end":71},"obj":"DNA_domain_or_region"},{"id":"T3","span":{"begin":195,"end":212},"obj":"other_name"},{"id":"T4","span":{"begin":220,"end":253},"obj":"DNA_domain_or_region"},{"id":"T5","span":{"begin":253,"end":256},"obj":"protein_molecule"},{"id":"T6","span":{"begin":302,"end":307},"obj":"DNA_domain_or_region"},{"id":"T7","span":{"begin":308,"end":311},"obj":"protein_molecule"},{"id":"T8","span":{"begin":374,"end":386},"obj":"DNA_family_or_group"},{"id":"T9","span":{"begin":483,"end":503},"obj":"protein_family_or_group"},{"id":"T10","span":{"begin":544,"end":551},"obj":"DNA_molecule"},{"id":"T11","span":{"begin":552,"end":555},"obj":"protein_molecule"},{"id":"T12","span":{"begin":604,"end":629},"obj":"cell_line"},{"id":"T13","span":{"begin":670,"end":691},"obj":"protein_family_or_group"},{"id":"T14","span":{"begin":692,"end":701},"obj":"protein_molecule"},{"id":"T15","span":{"begin":703,"end":709},"obj":"protein_molecule"},{"id":"T16","span":{"begin":733,"end":743},"obj":"protein_molecule"},{"id":"T17","span":{"begin":745,"end":755},"obj":"protein_molecule"},{"id":"T18","span":{"begin":768,"end":771},"obj":"protein_molecule"},{"id":"T19","span":{"begin":772,"end":789},"obj":"other_name"},{"id":"T20","span":{"begin":800,"end":813},"obj":"other_organic_compound"},{"id":"T21","span":{"begin":861,"end":874},"obj":"protein_molecule"},{"id":"T22","span":{"begin":904,"end":917},"obj":"other_organic_compound"},{"id":"T23","span":{"begin":947,"end":952},"obj":"protein_family_or_group"},{"id":"T24","span":{"begin":1005,"end":1008},"obj":"protein_molecule"},{"id":"T25","span":{"begin":1105,"end":1115},"obj":"protein_molecule"},{"id":"T26","span":{"begin":1170,"end":1191},"obj":"protein_family_or_group"},{"id":"T27","span":{"begin":1208,"end":1216},"obj":"protein_family_or_group"},{"id":"T28","span":{"begin":1250,"end":1253},"obj":"protein_molecule"}],"attributes":[{"subj":"T1","pred":"source","obj":"GENIAcorpus"},{"subj":"T2","pred":"source","obj":"GENIAcorpus"},{"subj":"T3","pred":"source","obj":"GENIAcorpus"},{"subj":"T4","pred":"source","obj":"GENIAcorpus"},{"subj":"T5","pred":"source","obj":"GENIAcorpus"},{"subj":"T6","pred":"source","obj":"GENIAcorpus"},{"subj":"T7","pred":"source","obj":"GENIAcorpus"},{"subj":"T8","pred":"source","obj":"GENIAcorpus"},{"subj":"T9","pred":"source","obj":"GENIAcorpus"},{"subj":"T10","pred":"source","obj":"GENIAcorpus"},{"subj":"T11","pred":"source","obj":"GENIAcorpus"},{"subj":"T12","pred":"source","obj":"GENIAcorpus"},{"subj":"T13","pred":"source","obj":"GENIAcorpus"},{"subj":"T14","pred":"source","obj":"GENIAcorpus"},{"subj":"T15","pred":"source","obj":"GENIAcorpus"},{"subj":"T16","pred":"source","obj":"GENIAcorpus"},{"subj":"T17","pred":"source","obj":"GENIAcorpus"},{"subj":"T18","pred":"source","obj":"GENIAcorpus"},{"subj":"T19","pred":"source","obj":"GENIAcorpus"},{"subj":"T20","pred":"source","obj":"GENIAcorpus"},{"subj":"T21","pred":"source","obj":"GENIAcorpus"},{"subj":"T22","pred":"source","obj":"GENIAcorpus"},{"subj":"T23","pred":"source","obj":"GENIAcorpus"},{"subj":"T24","pred":"source","obj":"GENIAcorpus"},{"subj":"T25","pred":"source","obj":"GENIAcorpus"},{"subj":"T26","pred":"source","obj":"GENIAcorpus"},{"subj":"T27","pred":"source","obj":"GENIAcorpus"},{"subj":"T28","pred":"source","obj":"GENIAcorpus"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"genia-medco-coref","color":"#e0ec93","default":true},{"id":"jnlpba-st-training","color":"#dd93ec"},{"id":"pubmed-sentences-benchmark","color":"#93ecc3"},{"id":"GENIAcorpus","color":"#eca993"}]}]}}