PubMed:9363920 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/9363920","sourcedb":"PubMed","sourceid":"9363920","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/9363920","text":"The activation of the JAK2/STAT5 pathway is commonly involved in signaling through the human IL-5 receptor.\nThe JAK (Janus kinase) family of protein tyrosine kinases and the STATs (signal transducers and activators of transcription) have been shown to be activated in response to a number of cytokines and growth factors. In this study, we evaluated the activation of JAK/STAT pathway upon human interleukin-5 (hIL-5) stimulation of two different hIL-5-responsive cell lines, hIL-5 receptor alpha-subunit (hIL-5R alpha) cDNA-transfected TF-1 (TF-h5R alpha) and butyric-acid-treated YY-1 (YY-Bu), and peripheral eosinophils. Immunoprecipitation and electrophoretic mobility shift analysis revealed that tyrosine phosphorylation of JAK2 and activation of STAT5 were induced upon stimulation with hIL-5 in all three cell types, while STAT1 activation was only observed in eosinophils. These results indicate that JAK2/STAT5 activation is a common JAK/STAT pathway for hIL-5-mediated signal in these 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