PubMed:8978306 JSON TXT

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Inflammaging

{"project":"Inflammaging","denotations":[{"id":"T1","span":{"begin":0,"end":72},"obj":"Sentence"},{"id":"T2","span":{"begin":73,"end":248},"obj":"Sentence"},{"id":"T3","span":{"begin":249,"end":452},"obj":"Sentence"},{"id":"T4","span":{"begin":453,"end":653},"obj":"Sentence"},{"id":"T5","span":{"begin":654,"end":810},"obj":"Sentence"},{"id":"T6","span":{"begin":811,"end":991},"obj":"Sentence"},{"id":"T7","span":{"begin":992,"end":1174},"obj":"Sentence"},{"id":"T8","span":{"begin":1175,"end":1231},"obj":"Sentence"},{"id":"T9","span":{"begin":1232,"end":1396},"obj":"Sentence"},{"id":"T10","span":{"begin":1397,"end":1620},"obj":"Sentence"},{"id":"T11","span":{"begin":1621,"end":1701},"obj":"Sentence"},{"id":"T12","span":{"begin":1702,"end":1913},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":72},"obj":"Sentence"},{"id":"T2","span":{"begin":73,"end":248},"obj":"Sentence"},{"id":"T3","span":{"begin":249,"end":452},"obj":"Sentence"},{"id":"T4","span":{"begin":453,"end":653},"obj":"Sentence"},{"id":"T5","span":{"begin":654,"end":810},"obj":"Sentence"},{"id":"T6","span":{"begin":811,"end":991},"obj":"Sentence"},{"id":"T7","span":{"begin":992,"end":1174},"obj":"Sentence"},{"id":"T8","span":{"begin":1175,"end":1231},"obj":"Sentence"},{"id":"T9","span":{"begin":1232,"end":1396},"obj":"Sentence"},{"id":"T10","span":{"begin":1397,"end":1620},"obj":"Sentence"},{"id":"T11","span":{"begin":1621,"end":1701},"obj":"Sentence"},{"id":"T12","span":{"begin":1702,"end":1913},"obj":"Sentence"}],"text":"Engagement of the Lewis X antigen (CD15) results in monocyte activation.\nWe previously reported that monocyte adhesion to tumor necrosis factor-alpha (TNF-alpha)-treated endothelial cells increased expression of tissue factor and CD36 on monocytes. Using immunological cross-linking to mimic receptor engagement by natural ligands, we now show that CD15 (Lewis X), a monocyte counter-receptor for endothelial selectins may participate in this response. We used cytokine production as a readout for monocyte activation and found that CD15 cross-linking induced TNF-alpha release from peripheral blood monocytes and cells from the monocytic cell line MM6. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) showed an increase in steady-state TNF-alpha mRNA after 3 to 4 hours of cross-linking. CD15 cross-linking also concomitantly increased interleukin-1 beta (IL-1 beta) mRNA, while no apparent change was observed in the levels of beta-actin mRNA, indicating specificity. To examine transcriptional regulation of cytokine genes by CD15 engagement, a CAT plasmid reporter construct containing IL-1 beta promoter/enhancer sequences was introduced into MM6. Subsequent cross-linking of CD15 increased CAT activity. CD15 engagement by monoclonal antibody also attenuated IL-1 beta transcript degradation, demonstrating that signaling via CD15 also had posttranscriptional effects. Nuclear extracts of anti-CD15 cross-linked cells demonstrated enhanced levels of the transcriptional factor activator protein-1, minimally changed nuclear factor-kappa B, and did not affect SV40 promoter specific protein-1. We conclude that engagement of CD15 on monocytes results in monocyte activation. In addition to its well-recognized adhesive role, CD15 may function as an important signaling molecule capable of initiating proinflammatory events in monocytes that come into contact with activated endothelium."}

jnlpba-st-training

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":122,"end":187},"obj":"cell_line"},{"id":"T2","span":{"begin":230,"end":234},"obj":"protein"},{"id":"T3","span":{"begin":367,"end":392},"obj":"protein"},{"id":"T4","span":{"begin":397,"end":418},"obj":"protein"},{"id":"T5","span":{"begin":560,"end":569},"obj":"protein"},{"id":"T6","span":{"begin":583,"end":609},"obj":"cell_type"},{"id":"T7","span":{"begin":629,"end":652},"obj":"cell_line"},{"id":"T8","span":{"begin":667,"end":688},"obj":"protein"},{"id":"T9","span":{"begin":759,"end":773},"obj":"RNA"},{"id":"T10","span":{"begin":859,"end":894},"obj":"RNA"},{"id":"T11","span":{"begin":951,"end":966},"obj":"RNA"},{"id":"T12","span":{"begin":1033,"end":1047},"obj":"DNA"},{"id":"T13","span":{"begin":1112,"end":1149},"obj":"DNA"},{"id":"T14","span":{"begin":1170,"end":1173},"obj":"cell_line"},{"id":"T15","span":{"begin":1218,"end":1221},"obj":"protein"},{"id":"T16","span":{"begin":1251,"end":1270},"obj":"protein"},{"id":"T17","span":{"begin":1287,"end":1296},"obj":"protein"},{"id":"T18","span":{"begin":1422,"end":1445},"obj":"cell_type"},{"id":"T19","span":{"begin":1482,"end":1524},"obj":"protein"},{"id":"T20","span":{"begin":1544,"end":1566},"obj":"protein"},{"id":"T21","span":{"begin":1587,"end":1619},"obj":"protein"},{"id":"T22","span":{"begin":1853,"end":1862},"obj":"cell_type"}],"text":"Engagement of the Lewis X antigen (CD15) results in monocyte activation.\nWe previously reported that monocyte adhesion to tumor necrosis factor-alpha (TNF-alpha)-treated endothelial cells increased expression of tissue factor and CD36 on monocytes. Using immunological cross-linking to mimic receptor engagement by natural ligands, we now show that CD15 (Lewis X), a monocyte counter-receptor for endothelial selectins may participate in this response. We used cytokine production as a readout for monocyte activation and found that CD15 cross-linking induced TNF-alpha release from peripheral blood monocytes and cells from the monocytic cell line MM6. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) showed an increase in steady-state TNF-alpha mRNA after 3 to 4 hours of cross-linking. CD15 cross-linking also concomitantly increased interleukin-1 beta (IL-1 beta) mRNA, while no apparent change was observed in the levels of beta-actin mRNA, indicating specificity. To examine transcriptional regulation of cytokine genes by CD15 engagement, a CAT plasmid reporter construct containing IL-1 beta promoter/enhancer sequences was introduced into MM6. Subsequent cross-linking of CD15 increased CAT activity. CD15 engagement by monoclonal antibody also attenuated IL-1 beta transcript degradation, demonstrating that signaling via CD15 also had posttranscriptional effects. Nuclear extracts of anti-CD15 cross-linked cells demonstrated enhanced levels of the transcriptional factor activator protein-1, minimally changed nuclear factor-kappa B, and did not affect SV40 promoter specific protein-1. We conclude that engagement of CD15 on monocytes results in monocyte activation. In addition to its well-recognized adhesive role, CD15 may function as an important signaling molecule capable of initiating proinflammatory events in monocytes that come into contact with activated endothelium."}

pubmed-sentences-benchmark

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":72},"obj":"Sentence"},{"id":"S2","span":{"begin":73,"end":248},"obj":"Sentence"},{"id":"S3","span":{"begin":249,"end":452},"obj":"Sentence"},{"id":"S4","span":{"begin":453,"end":653},"obj":"Sentence"},{"id":"S5","span":{"begin":654,"end":810},"obj":"Sentence"},{"id":"S6","span":{"begin":811,"end":991},"obj":"Sentence"},{"id":"S7","span":{"begin":992,"end":1174},"obj":"Sentence"},{"id":"S8","span":{"begin":1175,"end":1231},"obj":"Sentence"},{"id":"S9","span":{"begin":1232,"end":1396},"obj":"Sentence"},{"id":"S10","span":{"begin":1397,"end":1620},"obj":"Sentence"},{"id":"S11","span":{"begin":1621,"end":1701},"obj":"Sentence"},{"id":"S12","span":{"begin":1702,"end":1913},"obj":"Sentence"}],"text":"Engagement of the Lewis X antigen (CD15) results in monocyte activation.\nWe previously reported that monocyte adhesion to tumor necrosis factor-alpha (TNF-alpha)-treated endothelial cells increased expression of tissue factor and CD36 on monocytes. Using immunological cross-linking to mimic receptor engagement by natural ligands, we now show that CD15 (Lewis X), a monocyte counter-receptor for endothelial selectins may participate in this response. We used cytokine production as a readout for monocyte activation and found that CD15 cross-linking induced TNF-alpha release from peripheral blood monocytes and cells from the monocytic cell line MM6. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) showed an increase in steady-state TNF-alpha mRNA after 3 to 4 hours of cross-linking. CD15 cross-linking also concomitantly increased interleukin-1 beta (IL-1 beta) mRNA, while no apparent change was observed in the levels of beta-actin mRNA, indicating specificity. To examine transcriptional regulation of cytokine genes by CD15 engagement, a CAT plasmid reporter construct containing IL-1 beta promoter/enhancer sequences was introduced into MM6. Subsequent cross-linking of CD15 increased CAT activity. CD15 engagement by monoclonal antibody also attenuated IL-1 beta transcript degradation, demonstrating that signaling via CD15 also had posttranscriptional effects. Nuclear extracts of anti-CD15 cross-linked cells demonstrated enhanced levels of the transcriptional factor activator protein-1, minimally changed nuclear factor-kappa B, and did not affect SV40 promoter specific protein-1. We conclude that engagement of CD15 on monocytes results in monocyte activation. In addition to its well-recognized adhesive role, CD15 may function as an important signaling molecule capable of initiating proinflammatory events in monocytes that come into contact with activated endothelium."}

genia-medco-coref

GENIAcorpus