PubMed:8842711 JSONTXT

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Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":107,"end":117},"obj":"http://www.uniprot.org/uniprot/P16109"},{"id":"T2","span":{"begin":385,"end":395},"obj":"http://www.uniprot.org/uniprot/P16109"},{"id":"T3","span":{"begin":534,"end":544},"obj":"http://www.uniprot.org/uniprot/P16109"},{"id":"T4","span":{"begin":624,"end":634},"obj":"http://www.uniprot.org/uniprot/P16109"},{"id":"T5","span":{"begin":784,"end":794},"obj":"http://www.uniprot.org/uniprot/P16109"},{"id":"T6","span":{"begin":851,"end":861},"obj":"http://www.uniprot.org/uniprot/P16109"},{"id":"T7","span":{"begin":1232,"end":1242},"obj":"http://www.uniprot.org/uniprot/P16109"},{"id":"T8","span":{"begin":948,"end":952},"obj":"http://www.uniprot.org/uniprot/P61160"},{"id":"T9","span":{"begin":948,"end":952},"obj":"http://www.uniprot.org/uniprot/Q9UKU9"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":107,"end":117},"obj":"http://www.uniprot.org/uniprot/Q01102"},{"id":"T2","span":{"begin":385,"end":395},"obj":"http://www.uniprot.org/uniprot/Q01102"},{"id":"T3","span":{"begin":534,"end":544},"obj":"http://www.uniprot.org/uniprot/Q01102"},{"id":"T4","span":{"begin":624,"end":634},"obj":"http://www.uniprot.org/uniprot/Q01102"},{"id":"T5","span":{"begin":784,"end":794},"obj":"http://www.uniprot.org/uniprot/Q01102"},{"id":"T6","span":{"begin":851,"end":861},"obj":"http://www.uniprot.org/uniprot/Q01102"},{"id":"T7","span":{"begin":1232,"end":1242},"obj":"http://www.uniprot.org/uniprot/Q01102"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":264,"end":269},"obj":"http://purl.bioontology.org/ontology/STY/T025"},{"id":"T2","span":{"begin":657,"end":667},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/608684"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":63,"end":69},"obj":"http://purl.obolibrary.org/obo/GO_0097503"},{"id":"T2","span":{"begin":428,"end":434},"obj":"http://purl.obolibrary.org/obo/GO_0097503"},{"id":"T3","span":{"begin":160,"end":163},"obj":"http://purl.obolibrary.org/obo/GO_0034727"},{"id":"T4","span":{"begin":245,"end":248},"obj":"http://purl.obolibrary.org/obo/GO_0034727"},{"id":"T5","span":{"begin":190,"end":201},"obj":"http://purl.obolibrary.org/obo/GO_0032502"},{"id":"T6","span":{"begin":974,"end":983},"obj":"http://purl.obolibrary.org/obo/GO_0032502"},{"id":"T7","span":{"begin":335,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0002526"},{"id":"T8","span":{"begin":335,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0002673"},{"id":"T9","span":{"begin":335,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0002674"},{"id":"T10","span":{"begin":335,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0002675"},{"id":"T11","span":{"begin":341,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0006954"},{"id":"T12","span":{"begin":1205,"end":1218},"obj":"http://purl.obolibrary.org/obo/GO_0007155"},{"id":"T13","span":{"begin":1205,"end":1227},"obj":"http://purl.obolibrary.org/obo/GO_0033627"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GO-MF","denotations":[{"id":"T1","span":{"begin":109,"end":117},"obj":"http://purl.obolibrary.org/obo/GO_0030246"},{"id":"T2","span":{"begin":387,"end":395},"obj":"http://purl.obolibrary.org/obo/GO_0030246"},{"id":"T3","span":{"begin":536,"end":544},"obj":"http://purl.obolibrary.org/obo/GO_0030246"},{"id":"T4","span":{"begin":626,"end":634},"obj":"http://purl.obolibrary.org/obo/GO_0030246"},{"id":"T5","span":{"begin":786,"end":794},"obj":"http://purl.obolibrary.org/obo/GO_0030246"},{"id":"T6","span":{"begin":853,"end":861},"obj":"http://purl.obolibrary.org/obo/GO_0030246"},{"id":"T7","span":{"begin":697,"end":706},"obj":"http://purl.obolibrary.org/obo/GO_0030246"},{"id":"T8","span":{"begin":118,"end":128},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T9","span":{"begin":668,"end":678},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T10","span":{"begin":795,"end":805},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T11","span":{"begin":820,"end":830},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T12","span":{"begin":420,"end":426},"obj":"http://purl.obolibrary.org/obo/GO_0005488"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":264,"end":269},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T2","span":{"begin":838,"end":842},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3","span":{"begin":1205,"end":1209},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4","span":{"begin":838,"end":850},"obj":"http://purl.obolibrary.org/obo/GO_0009986"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":1316,"end":1321},"obj":"http://purl.obolibrary.org/obo/UBERON_0000948"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":365,"end":376},"obj":"http://edamontology.org/topic_0602"},{"id":"T2","span":{"begin":563,"end":570},"obj":"http://edamontology.org/topic_0634"},{"id":"T3","span":{"begin":635,"end":640},"obj":"http://edamontology.org/topic_3512"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":305,"end":323},"obj":"http://edamontology.org/operation_2446"},{"id":"T2","span":{"begin":316,"end":323},"obj":"http://edamontology.org/operation_2409"},{"id":"T3","span":{"begin":316,"end":323},"obj":"http://edamontology.org/operation_0004"},{"id":"T4","span":{"begin":716,"end":722},"obj":"http://edamontology.org/data_2048"},{"id":"T5","span":{"begin":740,"end":750},"obj":"http://edamontology.org/operation_3429"},{"id":"T6","span":{"begin":831,"end":837},"obj":"http://edamontology.org/operation_2423"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlycoBiology-MAT","denotations":[{"id":"T1","span":{"begin":1316,"end":1321},"obj":"http://purl.obolibrary.org/obo/MAT_0000036"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":1316,"end":1321},"obj":"http://purl.obolibrary.org/obo/UBERON_0000948"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":63,"end":75},"obj":"http://rdf.glycoinfo.org/glycan/G00053MO"},{"id":"T2","span":{"begin":428,"end":440},"obj":"http://rdf.glycoinfo.org/glycan/G00053MO"},{"id":"T3","span":{"begin":63,"end":77},"obj":"http://rdf.glycoinfo.org/glycan/G00054MO"},{"id":"T4","span":{"begin":428,"end":442},"obj":"http://rdf.glycoinfo.org/glycan/G00054MO"},{"id":"T5","span":{"begin":70,"end":75},"obj":"http://rdf.glycoinfo.org/glycan/G00047MO"},{"id":"T6","span":{"begin":435,"end":440},"obj":"http://rdf.glycoinfo.org/glycan/G00047MO"},{"id":"T7","span":{"begin":70,"end":77},"obj":"http://rdf.glycoinfo.org/glycan/G00051MO"},{"id":"T8","span":{"begin":435,"end":442},"obj":"http://rdf.glycoinfo.org/glycan/G00051MO"},{"id":"T9","span":{"begin":444,"end":448},"obj":"http://rdf.glycoinfo.org/glycan/G00054MO"},{"id":"T10","span":{"begin":1155,"end":1159},"obj":"http://rdf.glycoinfo.org/glycan/G00054MO"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"Lectin","denotations":[{"id":"Lectin_T1","span":{"begin":109,"end":117},"obj":"https://acgg.asia/db/lfdb/LfDB0142"},{"id":"Lectin_T2","span":{"begin":387,"end":395},"obj":"https://acgg.asia/db/lfdb/LfDB0142"},{"id":"Lectin_T3","span":{"begin":536,"end":544},"obj":"https://acgg.asia/db/lfdb/LfDB0142"},{"id":"Lectin_T4","span":{"begin":626,"end":634},"obj":"https://acgg.asia/db/lfdb/LfDB0142"},{"id":"Lectin_T5","span":{"begin":786,"end":794},"obj":"https://acgg.asia/db/lfdb/LfDB0142"},{"id":"Lectin_T6","span":{"begin":853,"end":861},"obj":"https://acgg.asia/db/lfdb/LfDB0142"},{"id":"Lectin_T7","span":{"begin":1234,"end":1242},"obj":"https://acgg.asia/db/lfdb/LfDB0142"},{"id":"Lectin_T8","span":{"begin":107,"end":117},"obj":"https://acgg.asia/db/lfdb/LfDB0013"},{"id":"Lectin_T9","span":{"begin":385,"end":395},"obj":"https://acgg.asia/db/lfdb/LfDB0013"},{"id":"Lectin_T10","span":{"begin":534,"end":544},"obj":"https://acgg.asia/db/lfdb/LfDB0013"},{"id":"Lectin_T11","span":{"begin":624,"end":634},"obj":"https://acgg.asia/db/lfdb/LfDB0013"},{"id":"Lectin_T12","span":{"begin":784,"end":794},"obj":"https://acgg.asia/db/lfdb/LfDB0013"},{"id":"Lectin_T13","span":{"begin":851,"end":861},"obj":"https://acgg.asia/db/lfdb/LfDB0013"},{"id":"Lectin_T14","span":{"begin":1232,"end":1242},"obj":"https://acgg.asia/db/lfdb/LfDB0013"},{"id":"Lectin_T15","span":{"begin":109,"end":117},"obj":"https://acgg.asia/db/lfdb/LfDB0043"},{"id":"Lectin_T16","span":{"begin":387,"end":395},"obj":"https://acgg.asia/db/lfdb/LfDB0043"},{"id":"Lectin_T17","span":{"begin":536,"end":544},"obj":"https://acgg.asia/db/lfdb/LfDB0043"},{"id":"Lectin_T18","span":{"begin":626,"end":634},"obj":"https://acgg.asia/db/lfdb/LfDB0043"},{"id":"Lectin_T19","span":{"begin":786,"end":794},"obj":"https://acgg.asia/db/lfdb/LfDB0043"},{"id":"Lectin_T20","span":{"begin":853,"end":861},"obj":"https://acgg.asia/db/lfdb/LfDB0043"},{"id":"Lectin_T21","span":{"begin":1234,"end":1242},"obj":"https://acgg.asia/db/lfdb/LfDB0043"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlycoBiology-Epitope","denotations":[{"id":"PD-GlycoEpitope-B_T1","span":{"begin":63,"end":75},"obj":"http://www.glycoepitope.jp/epitopes/EP0008"},{"id":"PD-GlycoEpitope-B_T2","span":{"begin":428,"end":440},"obj":"http://www.glycoepitope.jp/epitopes/EP0008"},{"id":"PD-GlycoEpitope-B_T3","span":{"begin":63,"end":77},"obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"PD-GlycoEpitope-B_T4","span":{"begin":428,"end":442},"obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"PD-GlycoEpitope-B_T5","span":{"begin":70,"end":77},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"},{"id":"PD-GlycoEpitope-B_T6","span":{"begin":435,"end":442},"obj":"http://www.glycoepitope.jp/epitopes/EP0011"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":17,"end":36},"obj":"Disease"},{"id":"T2","span":{"begin":53,"end":59},"obj":"Disease"},{"id":"T3","span":{"begin":225,"end":231},"obj":"Disease"},{"id":"T4","span":{"begin":1000,"end":1008},"obj":"Disease"},{"id":"T5","span":{"begin":1061,"end":1067},"obj":"Disease"},{"id":"T6","span":{"begin":1277,"end":1308},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0024644"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0021178"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0021178"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0005053"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0021178"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MONDO_0005203"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":63,"end":77},"obj":"Glycan"},{"id":"T2","span":{"begin":428,"end":442},"obj":"Glycan"},{"id":"T3","span":{"begin":444,"end":448},"obj":"Glycan"},{"id":"T4","span":{"begin":1155,"end":1159},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A5","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A6","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A7","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A8","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":63,"end":77},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":428,"end":442},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":444,"end":448},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":1155,"end":1159},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0012"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-MONDO","denotations":[{"id":"T1","span":{"begin":17,"end":36},"obj":"Disease"},{"id":"T2","span":{"begin":53,"end":59},"obj":"Disease"},{"id":"T3","span":{"begin":225,"end":231},"obj":"Disease"},{"id":"T4","span":{"begin":1000,"end":1008},"obj":"Disease"},{"id":"T5","span":{"begin":1061,"end":1067},"obj":"Disease"},{"id":"T6","span":{"begin":1277,"end":1308},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0024644"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0021178"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0021178"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0005053"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0021178"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MONDO_0005203"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-NCBITAXON","denotations":[{"id":"T1","span":{"begin":13,"end":16},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":103,"end":106},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":592,"end":596},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":613,"end":616},"obj":"OrganismTaxon"},{"id":"T9","span":{"begin":693,"end":696},"obj":"OrganismTaxon"},{"id":"T11","span":{"begin":780,"end":783},"obj":"OrganismTaxon"},{"id":"T13","span":{"begin":885,"end":888},"obj":"OrganismTaxon"},{"id":"T15","span":{"begin":1046,"end":1049},"obj":"OrganismTaxon"},{"id":"T17","span":{"begin":1312,"end":1315},"obj":"OrganismTaxon"}],"attributes":[{"id":"A5","pred":"db_id","subj":"T5","obj":"10114"},{"id":"A6","pred":"db_id","subj":"T5","obj":"10116"},{"id":"A7","pred":"db_id","subj":"T7","obj":"10114"},{"id":"A8","pred":"db_id","subj":"T7","obj":"10116"},{"id":"A9","pred":"db_id","subj":"T9","obj":"10114"},{"id":"A10","pred":"db_id","subj":"T9","obj":"10116"},{"id":"A11","pred":"db_id","subj":"T11","obj":"10114"},{"id":"A12","pred":"db_id","subj":"T11","obj":"10116"},{"id":"A13","pred":"db_id","subj":"T13","obj":"10114"},{"id":"A14","pred":"db_id","subj":"T13","obj":"10116"},{"id":"A15","pred":"db_id","subj":"T15","obj":"10114"},{"id":"A16","pred":"db_id","subj":"T15","obj":"10116"},{"id":"A17","pred":"db_id","subj":"T17","obj":"10114"},{"id":"A18","pred":"db_id","subj":"T17","obj":"10116"},{"id":"A1","pred":"db_id","subj":"T1","obj":"10114"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10116"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10114"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10116"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-CL","denotations":[{"id":"T1","span":{"begin":130,"end":158},"obj":"Cell"},{"id":"T8","span":{"begin":160,"end":163},"obj":"Cell"},{"id":"T9","span":{"begin":245,"end":248},"obj":"Cell"},{"id":"T10","span":{"begin":252,"end":269},"obj":"Cell"},{"id":"T11","span":{"begin":889,"end":898},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000041"},{"id":"A2","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000043"},{"id":"A3","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000094"},{"id":"A4","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000096"},{"id":"A5","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000767"},{"id":"A6","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000771"},{"id":"A7","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000775"},{"id":"A8","pred":"cl_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CL:0000041"},{"id":"A9","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000041"},{"id":"A10","pred":"cl_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/CL:0000115"},{"id":"A11","pred":"cl_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL:0000233"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":130,"end":158},"obj":"Body_part"},{"id":"T3","span":{"begin":160,"end":163},"obj":"Body_part"},{"id":"T4","span":{"begin":245,"end":248},"obj":"Body_part"},{"id":"T5","span":{"begin":252,"end":269},"obj":"Body_part"},{"id":"T6","span":{"begin":889,"end":898},"obj":"Body_part"},{"id":"T7","span":{"begin":1316,"end":1321},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000094"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000775"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000094"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000094"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL_0000115"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL_0000233"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0000948"},{"id":"A8","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0007100"},{"id":"A9","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0015228"},{"id":"A10","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0015230"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":1316,"end":1321},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000036"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":129},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":130,"end":232},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":233,"end":364},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":365,"end":510},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":511,"end":707},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":708,"end":813},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":814,"end":899},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":900,"end":1074},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1075,"end":1176},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1177,"end":1322},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":129},"obj":"Sentence"},{"id":"T2","span":{"begin":130,"end":232},"obj":"Sentence"},{"id":"T3","span":{"begin":233,"end":364},"obj":"Sentence"},{"id":"T4","span":{"begin":365,"end":510},"obj":"Sentence"},{"id":"T5","span":{"begin":511,"end":707},"obj":"Sentence"},{"id":"T6","span":{"begin":708,"end":813},"obj":"Sentence"},{"id":"T7","span":{"begin":814,"end":899},"obj":"Sentence"},{"id":"T8","span":{"begin":900,"end":1074},"obj":"Sentence"},{"id":"T9","span":{"begin":1075,"end":1176},"obj":"Sentence"},{"id":"T10","span":{"begin":1177,"end":1322},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":129},"obj":"Sentence"},{"id":"T2","span":{"begin":130,"end":232},"obj":"Sentence"},{"id":"T3","span":{"begin":233,"end":364},"obj":"Sentence"},{"id":"T4","span":{"begin":365,"end":510},"obj":"Sentence"},{"id":"T5","span":{"begin":511,"end":707},"obj":"Sentence"},{"id":"T6","span":{"begin":708,"end":813},"obj":"Sentence"},{"id":"T7","span":{"begin":814,"end":899},"obj":"Sentence"},{"id":"T8","span":{"begin":900,"end":1074},"obj":"Sentence"},{"id":"T9","span":{"begin":1075,"end":1176},"obj":"Sentence"},{"id":"T10","span":{"begin":1177,"end":1322},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":129},"obj":"Sentence"},{"id":"T2","span":{"begin":130,"end":232},"obj":"Sentence"},{"id":"T3","span":{"begin":233,"end":364},"obj":"Sentence"},{"id":"T4","span":{"begin":365,"end":510},"obj":"Sentence"},{"id":"T5","span":{"begin":511,"end":707},"obj":"Sentence"},{"id":"T6","span":{"begin":708,"end":813},"obj":"Sentence"},{"id":"T7","span":{"begin":814,"end":899},"obj":"Sentence"},{"id":"T8","span":{"begin":900,"end":1074},"obj":"Sentence"},{"id":"T9","span":{"begin":1075,"end":1176},"obj":"Sentence"},{"id":"T10","span":{"begin":1177,"end":1322},"obj":"Sentence"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":63,"end":77},"obj":"Glycan"},{"id":"T2","span":{"begin":428,"end":442},"obj":"Glycan"},{"id":"T3","span":{"begin":444,"end":448},"obj":"Glycan"},{"id":"T4","span":{"begin":1155,"end":1159},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A5","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A6","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A7","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G00054MO"},{"id":"A8","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00054MO"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"GlyCosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":63,"end":77},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":428,"end":442},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":444,"end":448},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":1155,"end":1159},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0012"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0012"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":13,"end":16},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":103,"end":106},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":592,"end":596},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":613,"end":616},"obj":"OrganismTaxon"},{"id":"T9","span":{"begin":693,"end":696},"obj":"OrganismTaxon"},{"id":"T11","span":{"begin":780,"end":783},"obj":"OrganismTaxon"},{"id":"T13","span":{"begin":885,"end":888},"obj":"OrganismTaxon"},{"id":"T15","span":{"begin":1046,"end":1049},"obj":"OrganismTaxon"},{"id":"T17","span":{"begin":1312,"end":1315},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10114"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10116"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10114"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10116"},{"id":"A5","pred":"db_id","subj":"T5","obj":"10114"},{"id":"A6","pred":"db_id","subj":"T5","obj":"10116"},{"id":"A7","pred":"db_id","subj":"T7","obj":"10114"},{"id":"A8","pred":"db_id","subj":"T7","obj":"10116"},{"id":"A9","pred":"db_id","subj":"T9","obj":"10114"},{"id":"A10","pred":"db_id","subj":"T9","obj":"10116"},{"id":"A11","pred":"db_id","subj":"T11","obj":"10114"},{"id":"A12","pred":"db_id","subj":"T11","obj":"10116"},{"id":"A13","pred":"db_id","subj":"T13","obj":"10114"},{"id":"A14","pred":"db_id","subj":"T13","obj":"10116"},{"id":"A15","pred":"db_id","subj":"T15","obj":"10114"},{"id":"A16","pred":"db_id","subj":"T15","obj":"10116"},{"id":"A17","pred":"db_id","subj":"T17","obj":"10114"},{"id":"A18","pred":"db_id","subj":"T17","obj":"10116"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":1316,"end":1321},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000036"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":130,"end":158},"obj":"Body_part"},{"id":"T3","span":{"begin":160,"end":163},"obj":"Body_part"},{"id":"T4","span":{"begin":245,"end":248},"obj":"Body_part"},{"id":"T5","span":{"begin":252,"end":269},"obj":"Body_part"},{"id":"T6","span":{"begin":889,"end":898},"obj":"Body_part"},{"id":"T7","span":{"begin":1316,"end":1321},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000094"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000775"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000094"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000094"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL_0000115"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL_0000233"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0000948"},{"id":"A8","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0007100"},{"id":"A9","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0015228"},{"id":"A10","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0015230"}],"text":"Reduction of rat myocardial ischemia and reperfusion injury by sialyl Lewis x oligosaccharide and anti-rat P-selectin antibodies.\nPolymorphonuclear leukocytes (PMN) are directly involved in development of ischemic myocardial injury. Adhesion of PMN to endothelial cells is an initial step that triggers a sequential process leading to acute inflammatory responses. Interaction between P-selectin and its oligosaccharide ligand, sialyl Lewis x (sLex), plays an important role in the early stage of the adhesion. To examine the role of P-selectin in various animal disease models especially in rats, we have cloned rat E- and P-selectin cDNAs and established monoclonal antibodies against these rat selectins. In this report, we describe the generation and characterization of anti-rat P-selectin antibodies (ARPs). These antibodies detect cell surface P-selectin on thrombin-stimulated rat platelets. More importantly, intravenous administration of ARP2-4 reduced infarction developed after 30 min of ischemia followed by 24 h of reperfusion in a rat myocardial injury model. In addition, similar protective effect was also observed by administration of a sLex-oligosaccharide. These results indicate that cell adhesion mediated via P-selectin is involved in the development of ischemia and reperfusion injury in rat heart."}