PubMed:8631981 JSONTXT

{"project":"ggdb-test","denotations":[{"id":"T1","span":{"begin":715,"end":718},"obj":"https://acgg.asia/db/ggdb/info/gg052"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

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cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"GGDB-2020","denotations":[{"id":"T1","span":{"begin":715,"end":718},"obj":"https://acgg.asia/db/ggdb/info/gg052"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

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{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":1337,"end":1343},"obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

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cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"Glycosmos6-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":30,"end":33},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T2","span":{"begin":52,"end":55},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T3","span":{"begin":129,"end":132},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T4","span":{"begin":284,"end":287},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T5","span":{"begin":416,"end":419},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T6","span":{"begin":641,"end":644},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T7","span":{"begin":689,"end":692},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T8","span":{"begin":1028,"end":1031},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T9","span":{"begin":1152,"end":1155},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"T10","span":{"begin":1213,"end":1216},"obj":"http://www.glycoepitope.jp/epitopes/EP0070"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":1337,"end":1343},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

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cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"GlyCosmos15-NCBITAXON","denotations":[{"id":"T1","span":{"begin":24,"end":29},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":1283,"end":1288},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":1391,"end":1401},"obj":"Body_part"},{"id":"T2","span":{"begin":1603,"end":1617},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005694"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0003714"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":1337,"end":1343},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":43},"obj":"Sentence"},{"id":"T2","span":{"begin":44,"end":91},"obj":"Sentence"},{"id":"T3","span":{"begin":92,"end":216},"obj":"Sentence"},{"id":"T4","span":{"begin":217,"end":493},"obj":"Sentence"},{"id":"T5","span":{"begin":494,"end":588},"obj":"Sentence"},{"id":"T6","span":{"begin":589,"end":666},"obj":"Sentence"},{"id":"T7","span":{"begin":667,"end":932},"obj":"Sentence"},{"id":"T8","span":{"begin":933,"end":1065},"obj":"Sentence"},{"id":"T9","span":{"begin":1066,"end":1243},"obj":"Sentence"},{"id":"T10","span":{"begin":1244,"end":1344},"obj":"Sentence"},{"id":"T11","span":{"begin":1345,"end":1417},"obj":"Sentence"},{"id":"T12","span":{"begin":1418,"end":1618},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":43},"obj":"Sentence"},{"id":"T2","span":{"begin":44,"end":91},"obj":"Sentence"},{"id":"T3","span":{"begin":92,"end":216},"obj":"Sentence"},{"id":"T4","span":{"begin":217,"end":493},"obj":"Sentence"},{"id":"T5","span":{"begin":494,"end":588},"obj":"Sentence"},{"id":"T6","span":{"begin":589,"end":666},"obj":"Sentence"},{"id":"T7","span":{"begin":667,"end":932},"obj":"Sentence"},{"id":"T8","span":{"begin":933,"end":1065},"obj":"Sentence"},{"id":"T9","span":{"begin":1066,"end":1243},"obj":"Sentence"},{"id":"T10","span":{"begin":1244,"end":1344},"obj":"Sentence"},{"id":"T11","span":{"begin":1345,"end":1417},"obj":"Sentence"},{"id":"T12","span":{"begin":1418,"end":1618},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":43},"obj":"Sentence"},{"id":"T2","span":{"begin":44,"end":91},"obj":"Sentence"},{"id":"T3","span":{"begin":92,"end":216},"obj":"Sentence"},{"id":"T4","span":{"begin":217,"end":588},"obj":"Sentence"},{"id":"T5","span":{"begin":589,"end":666},"obj":"Sentence"},{"id":"T6","span":{"begin":667,"end":932},"obj":"Sentence"},{"id":"T7","span":{"begin":933,"end":1065},"obj":"Sentence"},{"id":"T8","span":{"begin":1066,"end":1243},"obj":"Sentence"},{"id":"T9","span":{"begin":1244,"end":1344},"obj":"Sentence"},{"id":"T10","span":{"begin":1345,"end":1417},"obj":"Sentence"},{"id":"T11","span":{"begin":1418,"end":1618},"obj":"Sentence"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

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cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"GlyCosmos15-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":30,"end":33},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":52,"end":55},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":129,"end":132},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":284,"end":287},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T5","span":{"begin":416,"end":419},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T6","span":{"begin":641,"end":644},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T7","span":{"begin":689,"end":692},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T8","span":{"begin":1028,"end":1031},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T9","span":{"begin":1152,"end":1155},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T10","span":{"begin":1213,"end":1216},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A5","pred":"glycoepitope_id","subj":"T5","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A6","pred":"glycoepitope_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A7","pred":"glycoepitope_id","subj":"T7","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A8","pred":"glycoepitope_id","subj":"T8","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A9","pred":"glycoepitope_id","subj":"T9","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A10","pred":"glycoepitope_id","subj":"T10","obj":"http://www.glycoepitope.jp/epitopes/EP0070"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":24,"end":29},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":1283,"end":1288},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"GlyCosmos-GlycoEpitope","denotations":[{"id":"T1","span":{"begin":30,"end":33},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T2","span":{"begin":52,"end":55},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T3","span":{"begin":129,"end":132},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T4","span":{"begin":284,"end":287},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T5","span":{"begin":416,"end":419},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T6","span":{"begin":641,"end":644},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T7","span":{"begin":689,"end":692},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T8","span":{"begin":1028,"end":1031},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T9","span":{"begin":1152,"end":1155},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"},{"id":"T10","span":{"begin":1213,"end":1216},"obj":"http://purl.jp/bio/12/glyco/glycan#Glycan_epitope"}],"attributes":[{"id":"A1","pred":"glycoepitope_id","subj":"T1","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A2","pred":"glycoepitope_id","subj":"T2","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A3","pred":"glycoepitope_id","subj":"T3","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A4","pred":"glycoepitope_id","subj":"T4","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A5","pred":"glycoepitope_id","subj":"T5","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A6","pred":"glycoepitope_id","subj":"T6","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A7","pred":"glycoepitope_id","subj":"T7","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A8","pred":"glycoepitope_id","subj":"T8","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A9","pred":"glycoepitope_id","subj":"T9","obj":"http://www.glycoepitope.jp/epitopes/EP0070"},{"id":"A10","pred":"glycoepitope_id","subj":"T10","obj":"http://www.glycoepitope.jp/epitopes/EP0070"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1391,"end":1401},"obj":"Body_part"},{"id":"T2","span":{"begin":1603,"end":1617},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005694"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0003714"}],"text":"Expression cloning of a human GT3 synthase. GD3 AND GT3 are synthesized by a single enzyme.\nGangliosides of the C series such as GT3 are polysialylated glycosphingolipids whose synthesis is developmentally regulated. Here we report the expression cDNA cloning and characterization of GT3 synthase that adds the second alpha-2,8-sialic acid to GD3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, thus forming GT3, NeuNAcalpha2--\u003e8NeuNAcalpha2--\u003e8NeuNAc alpha2--\u003e3Galbeta1--\u003e 4Glc--\u003eCer. Unexpectedly, the cloned cDNA was found to be identical to the cDNA that encodes GD3 synthase. The newly identified enzyme was therefore named GD3/GT3 synthase (GD3/GT3ST). GD3/GT3ST synthesized GT3 most efficiently when GM3, NeuNAcalpha2--\u003e3Galbeta1--\u003e4Glc--\u003eCer, was incubated as an acceptor, indicating that GD3/GT3ST is a polysialyltransferase that can transfer more than one sialic acid residue via alpha-2,8 linkage to gangliosides. Moreover, a longer period of incubation of GD3 with GD3/GT3ST produced a significant amount of GT3 and higher polysialogangliosides. Among various cell lines expressing GD3/GT3ST, higher polysialogangliosides including GT3 were detected only in cell lines where the amount of GD3/GT3 mRNA is sufficiently high. The expression of GD3/GT3ST mRNA among human tissues is highly restricted to fetal and adult brains. The GD3/GT3ST gene was found to be located at chromosome 12, region p12. Taken together, these results indicate that C series polysialogangliosides are synthesized by a ganglioside-specific polysialyltransferase, GD3/GT3ST, that is specifically expressed in neural tissues."}