PubMed:8621538 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":156},"obj":"Sentence"},{"id":"T2","span":{"begin":157,"end":315},"obj":"Sentence"},{"id":"T3","span":{"begin":316,"end":513},"obj":"Sentence"},{"id":"T4","span":{"begin":514,"end":711},"obj":"Sentence"},{"id":"T5","span":{"begin":712,"end":822},"obj":"Sentence"},{"id":"T6","span":{"begin":823,"end":1006},"obj":"Sentence"},{"id":"T7","span":{"begin":1007,"end":1095},"obj":"Sentence"},{"id":"T8","span":{"begin":1096,"end":1236},"obj":"Sentence"},{"id":"T9","span":{"begin":1237,"end":1442},"obj":"Sentence"},{"id":"T10","span":{"begin":1443,"end":1549},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":156},"obj":"Sentence"},{"id":"T2","span":{"begin":157,"end":315},"obj":"Sentence"},{"id":"T3","span":{"begin":316,"end":513},"obj":"Sentence"},{"id":"T4","span":{"begin":514,"end":711},"obj":"Sentence"},{"id":"T5","span":{"begin":712,"end":822},"obj":"Sentence"},{"id":"T6","span":{"begin":823,"end":1006},"obj":"Sentence"},{"id":"T7","span":{"begin":1007,"end":1095},"obj":"Sentence"},{"id":"T8","span":{"begin":1096,"end":1236},"obj":"Sentence"},{"id":"T9","span":{"begin":1237,"end":1442},"obj":"Sentence"},{"id":"T10","span":{"begin":1443,"end":1549},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":612,"end":620},"obj":"http://purl.obolibrary.org/obo/MAT_0000491"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":18,"end":43},"obj":"DNA"},{"id":"T2","span":{"begin":51,"end":59},"obj":"DNA"},{"id":"T3","span":{"begin":98,"end":155},"obj":"protein"},{"id":"T4","span":{"begin":187,"end":195},"obj":"DNA"},{"id":"T5","span":{"begin":207,"end":232},"obj":"DNA"},{"id":"T6","span":{"begin":234,"end":238},"obj":"DNA"},{"id":"T7","span":{"begin":287,"end":314},"obj":"DNA"},{"id":"T8","span":{"begin":343,"end":347},"obj":"DNA"},{"id":"T9","span":{"begin":374,"end":390},"obj":"DNA"},{"id":"T10","span":{"begin":410,"end":417},"obj":"cell_type"},{"id":"T11","span":{"begin":514,"end":521},"obj":"protein"},{"id":"T12","span":{"begin":550,"end":565},"obj":"protein"},{"id":"T13","span":{"begin":571,"end":594},"obj":"cell_type"},{"id":"T14","span":{"begin":603,"end":625},"obj":"DNA"},{"id":"T15","span":{"begin":627,"end":634},"obj":"DNA"},{"id":"T16","span":{"begin":642,"end":662},"obj":"DNA"},{"id":"T17","span":{"begin":664,"end":672},"obj":"DNA"},{"id":"T18","span":{"begin":685,"end":710},"obj":"DNA"},{"id":"T19","span":{"begin":712,"end":727},"obj":"protein"},{"id":"T20","span":{"begin":791,"end":799},"obj":"DNA"},{"id":"T21","span":{"begin":813,"end":821},"obj":"DNA"},{"id":"T22","span":{"begin":823,"end":831},"obj":"DNA"},{"id":"T23","span":{"begin":867,"end":882},"obj":"DNA"},{"id":"T24","span":{"begin":938,"end":966},"obj":"protein"},{"id":"T25","span":{"begin":1017,"end":1043},"obj":"DNA"},{"id":"T26","span":{"begin":1068,"end":1091},"obj":"protein"},{"id":"T27","span":{"begin":1100,"end":1118},"obj":"DNA"},{"id":"T28","span":{"begin":1132,"end":1156},"obj":"protein"},{"id":"T29","span":{"begin":1162,"end":1170},"obj":"DNA"},{"id":"T30","span":{"begin":1280,"end":1288},"obj":"DNA"},{"id":"T31","span":{"begin":1300,"end":1332},"obj":"DNA"},{"id":"T32","span":{"begin":1429,"end":1441},"obj":"DNA"},{"id":"T33","span":{"begin":1499,"end":1511},"obj":"protein"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":156},"obj":"Sentence"},{"id":"S2","span":{"begin":157,"end":315},"obj":"Sentence"},{"id":"S3","span":{"begin":316,"end":513},"obj":"Sentence"},{"id":"S4","span":{"begin":514,"end":711},"obj":"Sentence"},{"id":"S5","span":{"begin":712,"end":822},"obj":"Sentence"},{"id":"S6","span":{"begin":823,"end":1006},"obj":"Sentence"},{"id":"S7","span":{"begin":1007,"end":1095},"obj":"Sentence"},{"id":"S8","span":{"begin":1096,"end":1236},"obj":"Sentence"},{"id":"S9","span":{"begin":1237,"end":1442},"obj":"Sentence"},{"id":"S10","span":{"begin":1443,"end":1549},"obj":"Sentence"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"genia-medco-coref","denotations":[{"id":"C2","span":{"begin":47,"end":92},"obj":"NP"},{"id":"C1","span":{"begin":16,"end":92},"obj":"NP"},{"id":"C3","span":{"begin":157,"end":195},"obj":"NP"},{"id":"C4","span":{"begin":205,"end":239},"obj":"NP"},{"id":"C5","span":{"begin":240,"end":244},"obj":"NP"},{"id":"C6","span":{"begin":316,"end":339},"obj":"NP"},{"id":"C7","span":{"begin":343,"end":347},"obj":"NP"},{"id":"C8","span":{"begin":358,"end":362},"obj":"NP"},{"id":"C9","span":{"begin":366,"end":390},"obj":"NP"},{"id":"C10","span":{"begin":432,"end":437},"obj":"NP"},{"id":"C11","span":{"begin":467,"end":512},"obj":"NP"},{"id":"C12","span":{"begin":601,"end":625},"obj":"NP"},{"id":"C13","span":{"begin":627,"end":634},"obj":"NP"},{"id":"C14","span":{"begin":640,"end":662},"obj":"NP"},{"id":"C15","span":{"begin":664,"end":672},"obj":"NP"},{"id":"C16","span":{"begin":783,"end":789},"obj":"NP"},{"id":"C17","span":{"begin":791,"end":799},"obj":"NP"},{"id":"C18","span":{"begin":813,"end":821},"obj":"NP"},{"id":"C19","span":{"begin":823,"end":831},"obj":"NP"},{"id":"C20","span":{"begin":938,"end":942},"obj":"NP"},{"id":"C21","span":{"begin":1064,"end":1094},"obj":"NP"},{"id":"C22","span":{"begin":1132,"end":1156},"obj":"NP"},{"id":"C23","span":{"begin":1162,"end":1170},"obj":"NP"},{"id":"C24","span":{"begin":1280,"end":1288},"obj":"NP"},{"id":"C25","span":{"begin":1292,"end":1332},"obj":"NP"},{"id":"C26","span":{"begin":1333,"end":1337},"obj":"NP"},{"id":"C27","span":{"begin":1352,"end":1355},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C3","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C4","obj":"C1"},{"id":"R3","pred":"coref-relat","subj":"C5","obj":"C4"},{"id":"R4","pred":"coref-ident","subj":"C7","obj":"C4"},{"id":"R5","pred":"coref-pron","subj":"C8","obj":"C6"},{"id":"R6","pred":"coref-pron","subj":"C10","obj":"C9"},{"id":"R7","pred":"coref-appos","subj":"C13","obj":"C12"},{"id":"R8","pred":"coref-appos","subj":"C15","obj":"C14"},{"id":"R9","pred":"coref-appos","subj":"C17","obj":"C16"},{"id":"R10","pred":"coref-ident","subj":"C18","obj":"C15"},{"id":"R11","pred":"coref-ident","subj":"C19","obj":"C18"},{"id":"R12","pred":"coref-ident","subj":"C20","obj":"C11"},{"id":"R13","pred":"coref-ident","subj":"C22","obj":"C21"},{"id":"R14","pred":"coref-ident","subj":"C23","obj":"C19"},{"id":"R15","pred":"coref-ident","subj":"C24","obj":"C23"},{"id":"R16","pred":"coref-relat","subj":"C26","obj":"C25"},{"id":"R17","pred":"coref-pron","subj":"C27","obj":"C24"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":18,"end":43},"obj":"DNA_domain_or_region"},{"id":"T2","span":{"begin":51,"end":59},"obj":"DNA_domain_or_region"},{"id":"T3","span":{"begin":67,"end":92},"obj":"virus"},{"id":"T4","span":{"begin":98,"end":155},"obj":"protein_family_or_group"},{"id":"T5","span":{"begin":161,"end":186},"obj":"virus"},{"id":"T6","span":{"begin":187,"end":195},"obj":"DNA_domain_or_region"},{"id":"T7","span":{"begin":207,"end":232},"obj":"DNA_domain_or_region"},{"id":"T8","span":{"begin":234,"end":238},"obj":"DNA_domain_or_region"},{"id":"T9","span":{"begin":287,"end":314},"obj":"DNA_domain_or_region"},{"id":"T10","span":{"begin":343,"end":347},"obj":"DNA_domain_or_region"},{"id":"T11","span":{"begin":374,"end":390},"obj":"DNA_family_or_group"},{"id":"T12","span":{"begin":410,"end":417},"obj":"cell_type"},{"id":"T13","span":{"begin":471,"end":493},"obj":"other_organic_compound"},{"id":"T14","span":{"begin":494,"end":506},"obj":"other_organic_compound"},{"id":"T15","span":{"begin":508,"end":511},"obj":"other_organic_compound"},{"id":"T16","span":{"begin":514,"end":521},"obj":"protein_molecule"},{"id":"T17","span":{"begin":550,"end":565},"obj":"protein_family_or_group"},{"id":"T18","span":{"begin":571,"end":594},"obj":"cell_type"},{"id":"T19","span":{"begin":603,"end":625},"obj":"DNA_domain_or_region"},{"id":"T20","span":{"begin":627,"end":634},"obj":"DNA_domain_or_region"},{"id":"T21","span":{"begin":642,"end":662},"obj":"DNA_domain_or_region"},{"id":"T22","span":{"begin":664,"end":672},"obj":"DNA_domain_or_region"},{"id":"T23","span":{"begin":685,"end":710},"obj":"DNA_domain_or_region"},{"id":"T24","span":{"begin":712,"end":727},"obj":"protein_family_or_group"},{"id":"T25","span":{"begin":791,"end":799},"obj":"DNA_domain_or_region"},{"id":"T26","span":{"begin":813,"end":821},"obj":"DNA_domain_or_region"},{"id":"T27","span":{"begin":823,"end":831},"obj":"DNA_domain_or_region"},{"id":"T28","span":{"begin":867,"end":882},"obj":"DNA_domain_or_region"},{"id":"T29","span":{"begin":888,"end":906},"obj":"other_name"},{"id":"T30","span":{"begin":938,"end":941},"obj":"other_organic_compound"},{"id":"T31","span":{"begin":985,"end":1005},"obj":"other_name"},{"id":"T32","span":{"begin":1068,"end":1076},"obj":"DNA_domain_or_region"},{"id":"T33","span":{"begin":1100,"end":1118},"obj":"DNA_domain_or_region"},{"id":"T34","span":{"begin":1132,"end":1140},"obj":"DNA_domain_or_region"},{"id":"T35","span":{"begin":1162,"end":1170},"obj":"DNA_domain_or_region"},{"id":"T36","span":{"begin":1225,"end":1235},"obj":"polynucleotide"},{"id":"T37","span":{"begin":1280,"end":1288},"obj":"DNA_domain_or_region"},{"id":"T38","span":{"begin":1300,"end":1332},"obj":"DNA_family_or_group"},{"id":"T39","span":{"begin":1406,"end":1409},"obj":"other_organic_compound"},{"id":"T40","span":{"begin":1429,"end":1441},"obj":"DNA_domain_or_region"},{"id":"T41","span":{"begin":1477,"end":1481},"obj":"virus"},{"id":"T42","span":{"begin":1499,"end":1511},"obj":"protein_family_or_group"},{"id":"T43","span":{"begin":1532,"end":1548},"obj":"other_name"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":612,"end":620},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000491"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":81,"end":86},"obj":"Phenotype"},{"id":"T2","span":{"begin":175,"end":180},"obj":"Phenotype"},{"id":"T3","span":{"begin":1532,"end":1548},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002664"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0002664"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"HP:0012190"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":73,"end":86},"obj":"Disease"},{"id":"T2","span":{"begin":167,"end":180},"obj":"Disease"},{"id":"T3","span":{"begin":1532,"end":1548},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0007254"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0007254"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0015760"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":67,"end":92},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":161,"end":186},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"11757"},{"id":"A2","pred":"db_id","subj":"T2","obj":"11757"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":120,"end":126},"obj":"Body_part"},{"id":"T2","span":{"begin":581,"end":594},"obj":"Body_part"},{"id":"T3","span":{"begin":952,"end":958},"obj":"Body_part"},{"id":"T4","span":{"begin":1532,"end":1538},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000084"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":120,"end":126},"obj":"Cell"},{"id":"T2","span":{"begin":410,"end":417},"obj":"Cell"},{"id":"T3","span":{"begin":581,"end":594},"obj":"Cell"},{"id":"T4","span":{"begin":952,"end":958},"obj":"Cell"},{"id":"T5","span":{"begin":1532,"end":1538},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000084"}],"text":"Interactions of a transcriptional activator in the env gene of the mouse mammary tumor virus with activation-dependent, T cell-specific transacting factors.\nThe mouse mammary tumor virus env gene contains a transcriptional activator (META) that can control transcription of the adjacent long terminal repeat region. Transcriptional control by META parallels that of several lymphokine genes, being specific to T cells, dependent on their activation, and inhibited by the immunosuppressive drug cyclosporine (CsA). DNase I footprinting indicated that nuclear factors from activated T lymphocytes bound a promoter-proximal site, META(P), and a promoter-distal site, META(D+), within the 400-base pair META region. Nuclear factors from unstimulated, but not from activated cells, bound a site, META(D-), adjacent to META(D+). META(D+) directed transcription of a linked luciferase gene, and gel shift analysis revealed binding of inducible, CsA-sensitive T cell factors, in parallel with transfection results. Authentic NFAT and NF-kappaB targets did not compete for the META(D+) binding factor(s). The SV40 core sequence competed for META(D+) binding factors, but META(D+) failed to compete for the complexes obtained with the SV40 probe. Our results, taken together, indicate that META(D+) is a novel transcriptional enhancer element that is similar in its cell-type specificity, activation dependence, and CsA sensitivity to the NFAT element. It may be relevant to the role of MMTV in expression of Mls antigens or the induction of T cell lymphomas."}