PubMed:8621390 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":94},"obj":"Sentence"},{"id":"T2","span":{"begin":95,"end":173},"obj":"Sentence"},{"id":"T3","span":{"begin":174,"end":291},"obj":"Sentence"},{"id":"T4","span":{"begin":292,"end":440},"obj":"Sentence"},{"id":"T5","span":{"begin":441,"end":598},"obj":"Sentence"},{"id":"T6","span":{"begin":599,"end":839},"obj":"Sentence"},{"id":"T7","span":{"begin":840,"end":965},"obj":"Sentence"},{"id":"T8","span":{"begin":966,"end":1168},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":94},"obj":"Sentence"},{"id":"T2","span":{"begin":95,"end":173},"obj":"Sentence"},{"id":"T3","span":{"begin":174,"end":291},"obj":"Sentence"},{"id":"T4","span":{"begin":292,"end":440},"obj":"Sentence"},{"id":"T5","span":{"begin":441,"end":598},"obj":"Sentence"},{"id":"T6","span":{"begin":599,"end":839},"obj":"Sentence"},{"id":"T7","span":{"begin":840,"end":965},"obj":"Sentence"},{"id":"T8","span":{"begin":966,"end":1168},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":80,"end":85},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T2","span":{"begin":80,"end":85},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T3","span":{"begin":555,"end":563},"obj":"http://purl.obolibrary.org/obo/MAT_0000491"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":34,"end":52},"obj":"DNA"},{"id":"T2","span":{"begin":63,"end":93},"obj":"cell_type"},{"id":"T3","span":{"begin":153,"end":172},"obj":"cell_type"},{"id":"T4","span":{"begin":213,"end":233},"obj":"cell_type"},{"id":"T5","span":{"begin":292,"end":321},"obj":"DNA"},{"id":"T6","span":{"begin":321,"end":335},"obj":"DNA"},{"id":"T7","span":{"begin":367,"end":382},"obj":"DNA"},{"id":"T8","span":{"begin":398,"end":413},"obj":"protein"},{"id":"T9","span":{"begin":458,"end":479},"obj":"DNA"},{"id":"T10","span":{"begin":500,"end":528},"obj":"DNA"},{"id":"T11","span":{"begin":532,"end":546},"obj":"cell_type"},{"id":"T12","span":{"begin":555,"end":573},"obj":"DNA"},{"id":"T13","span":{"begin":582,"end":597},"obj":"DNA"},{"id":"T14","span":{"begin":629,"end":647},"obj":"protein"},{"id":"T15","span":{"begin":651,"end":668},"obj":"protein"},{"id":"T16","span":{"begin":710,"end":724},"obj":"protein"},{"id":"T17","span":{"begin":728,"end":749},"obj":"cell_type"},{"id":"T18","span":{"begin":753,"end":795},"obj":"cell_type"},{"id":"T19","span":{"begin":826,"end":838},"obj":"DNA"},{"id":"T20","span":{"begin":859,"end":899},"obj":"DNA"},{"id":"T21","span":{"begin":927,"end":941},"obj":"cell_type"},{"id":"T22","span":{"begin":950,"end":964},"obj":"cell_line"},{"id":"T23","span":{"begin":983,"end":987},"obj":"protein"},{"id":"T24","span":{"begin":1026,"end":1042},"obj":"cell_line"},{"id":"T25","span":{"begin":1057,"end":1071},"obj":"cell_type"},{"id":"T26","span":{"begin":1134,"end":1146},"obj":"DNA"},{"id":"T27","span":{"begin":1154,"end":1167},"obj":"DNA"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":94},"obj":"Sentence"},{"id":"S2","span":{"begin":95,"end":173},"obj":"Sentence"},{"id":"S3","span":{"begin":174,"end":291},"obj":"Sentence"},{"id":"S4","span":{"begin":292,"end":440},"obj":"Sentence"},{"id":"S5","span":{"begin":441,"end":598},"obj":"Sentence"},{"id":"S6","span":{"begin":599,"end":839},"obj":"Sentence"},{"id":"S7","span":{"begin":840,"end":965},"obj":"Sentence"},{"id":"S8","span":{"begin":966,"end":1168},"obj":"Sentence"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":56,"end":93},"obj":"NP"},{"id":"C2","span":{"begin":213,"end":233},"obj":"NP"},{"id":"C4","span":{"begin":532,"end":546},"obj":"NP"},{"id":"C3","span":{"begin":481,"end":546},"obj":"NP"},{"id":"C5","span":{"begin":551,"end":597},"obj":"NP"},{"id":"C6","span":{"begin":817,"end":838},"obj":"NP"},{"id":"C7","span":{"begin":927,"end":941},"obj":"NP"},{"id":"C8","span":{"begin":1057,"end":1071},"obj":"NP"},{"id":"C9","span":{"begin":1125,"end":1167},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C2","obj":"C1"},{"id":"R2","pred":"coref-ident","subj":"C4","obj":"C2"},{"id":"R3","pred":"coref-ident","subj":"C5","obj":"C3"},{"id":"R4","pred":"coref-ident","subj":"C6","obj":"C3"},{"id":"R5","pred":"coref-ident","subj":"C7","obj":"C4"},{"id":"R6","pred":"coref-ident","subj":"C8","obj":"C7"},{"id":"R7","pred":"coref-ident","subj":"C9","obj":"C6"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":26},"obj":"other_name"},{"id":"T2","span":{"begin":34,"end":47},"obj":"protein_molecule"},{"id":"T3","span":{"begin":63,"end":93},"obj":"cell_type"},{"id":"T4","span":{"begin":153,"end":172},"obj":"cell_type"},{"id":"T5","span":{"begin":213,"end":233},"obj":"cell_type"},{"id":"T6","span":{"begin":268,"end":290},"obj":"other_name"},{"id":"T7","span":{"begin":292,"end":307},"obj":"DNA_domain_or_region"},{"id":"T8","span":{"begin":307,"end":311},"obj":"protein_molecule"},{"id":"T9","span":{"begin":321,"end":335},"obj":"DNA_family_or_group"},{"id":"T10","span":{"begin":367,"end":382},"obj":"DNA_family_or_group"},{"id":"T11","span":{"begin":398,"end":413},"obj":"protein_molecule"},{"id":"T12","span":{"begin":418,"end":439},"obj":"other_name"},{"id":"T13","span":{"begin":458,"end":479},"obj":"DNA_family_or_group"},{"id":"T14","span":{"begin":500,"end":504},"obj":"protein_molecule"},{"id":"T15","span":{"begin":532,"end":546},"obj":"cell_type"},{"id":"T16","span":{"begin":555,"end":563},"obj":"DNA_domain_or_region"},{"id":"T17","span":{"begin":564,"end":568},"obj":"protein_molecule"},{"id":"T18","span":{"begin":582,"end":592},"obj":"protein_molecule"},{"id":"T19","span":{"begin":629,"end":647},"obj":"protein_molecule"},{"id":"T20","span":{"begin":651,"end":668},"obj":"protein_family_or_group"},{"id":"T21","span":{"begin":674,"end":687},"obj":"other_name"},{"id":"T22","span":{"begin":710,"end":724},"obj":"protein_family_or_group"},{"id":"T23","span":{"begin":728,"end":749},"obj":"cell_type"},{"id":"T24","span":{"begin":753,"end":795},"obj":"cell_type"},{"id":"T25","span":{"begin":826,"end":838},"obj":"DNA_family_or_group"},{"id":"T26","span":{"begin":859,"end":899},"obj":"DNA_domain_or_region"},{"id":"T27","span":{"begin":927,"end":941},"obj":"cell_type"},{"id":"T28","span":{"begin":950,"end":964},"obj":"cell_line"},{"id":"T29","span":{"begin":983,"end":987},"obj":"protein_molecule"},{"id":"T30","span":{"begin":1026,"end":1042},"obj":"cell_line"},{"id":"T31","span":{"begin":1057,"end":1071},"obj":"cell_type"},{"id":"T32","span":{"begin":1091,"end":1112},"obj":"other_name"},{"id":"T33","span":{"begin":1134,"end":1146},"obj":"DNA_family_or_group"},{"id":"T34","span":{"begin":1154,"end":1158},"obj":"protein_molecule"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":80,"end":85},"obj":"Body_part"},{"id":"T3","span":{"begin":555,"end":563},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A2","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000491"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":1026,"end":1031},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002664"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":1026,"end":1031},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":63,"end":68},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":220,"end":225},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":80,"end":85},"obj":"Body_part"},{"id":"T2","span":{"begin":398,"end":404},"obj":"Body_part"},{"id":"T3","span":{"begin":887,"end":893},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000084"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":86,"end":93},"obj":"Cell"},{"id":"T2","span":{"begin":165,"end":172},"obj":"Cell"},{"id":"T3","span":{"begin":226,"end":233},"obj":"Cell"},{"id":"T4","span":{"begin":398,"end":404},"obj":"Cell"},{"id":"T5","span":{"begin":539,"end":546},"obj":"Cell"},{"id":"T6","span":{"begin":741,"end":748},"obj":"Cell"},{"id":"T7","span":{"begin":887,"end":893},"obj":"Cell"},{"id":"T8","span":{"begin":934,"end":941},"obj":"Cell"},{"id":"T9","span":{"begin":957,"end":964},"obj":"Cell"},{"id":"T10","span":{"begin":1026,"end":1036},"obj":"Cell"},{"id":"T11","span":{"begin":1064,"end":1071},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A6","pred":"cl_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/CL:0000236"},{"id":"A7","pred":"cl_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A8","pred":"cl_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A9","pred":"cl_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A10","pred":"cl_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/CL:0001063"},{"id":"A11","pred":"cl_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/CL:0000084"}],"text":"Transcriptional regulation of the interleukin-2 gene in normal human peripheral blood T cells. Convergence of costimulatory signals and differences from transformed T cells.\nTo study transcriptional regulation in normal human T cells, we have optimized conditions for transient transfection. Interleukin-2 (IL-2) promoter-reporter gene behavior closely parallels the endogenous gene in response to T cell receptor and costimulatory signals. As assessed with mutagenized promoters, the most important IL-2 cis-regulatory elements in normal T cells are the proximal AP-1 site and the NF- kappaB site. Both primary activation, with phytohemagglutinin or antibodies to CD3, and costimulation, provided by pairs of CD2 antibodies or B7-positive (B cells) or B7-negative (endothelial) accessory cells, are mediated through the same cis-elements. Interestingly, the nuclear factor of activated T cell sites are much less important in normal T cells than in Jurkat T cells. We conclude that IL-2 transcriptional regulation differs in tumor cell lines compared with normal T cells and that different costimulatory signals converge on the same cis-elements in the IL-2 promoter."}