Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-96 |
Sentence |
denotes |
Transcriptional modulation of platelet-activating factor receptor gene expression by cyclic AMP. |
T2 |
97-216 |
Sentence |
denotes |
We examined the effects of increasing intracellular cyclic AMP levels on the expression of human PAF receptor (hPAF-R). |
T3 |
217-293 |
Sentence |
denotes |
Peripheral blood monocytes constitutively expressed hPAF-R mRNA transcripts. |
T4 |
294-463 |
Sentence |
denotes |
A transiently elevated intracellular concentration of AMP induced with prostaglandin E2, cholera toxin, or forskolin was a sufficient signal to inhibit PAF-R expression. |
T5 |
464-680 |
Sentence |
denotes |
To determine the mechanisms of this inhibition, human monocytes were treated with dibutyryl cAMP, a cell-permeable cAMP analogue. cAMP reduced the expression of hPAF-R in a concentration- and a time-dependent manner. |
T6 |
681-750 |
Sentence |
denotes |
The effect was seen as early as 1 h and was essentially total by 4 h. |
T7 |
751-871 |
Sentence |
denotes |
Stability of hPAF-R mRNA was not markedly decreased by cAMP, as assessed by measuring the half-lives of the transcripts. |
T8 |
872-991 |
Sentence |
denotes |
Moreover, the nuclear transcription rate of the hPAF-R gene was reduced as early as 30 min after stimulation with cAMP. |
T9 |
992-1296 |
Sentence |
denotes |
The inhibition of hPAF-R mRNA accumulation was associated with diminished responsiveness to PAF, as assayed by intracellular Ca2+ fluxes, decreased number of binding sites, and decreased hPAF-R protein expression on the cell surface, as assessed by flow cytometry using a polyclonal anti-hPAF-R antibody. |
T10 |
1297-1455 |
Sentence |
denotes |
These data indicate that PAF-R expression can be regulated at the transcriptional and possibly post-transcriptional levels by elevation of intracellular cAMP. |
T1 |
0-96 |
Sentence |
denotes |
Transcriptional modulation of platelet-activating factor receptor gene expression by cyclic AMP. |
T2 |
97-216 |
Sentence |
denotes |
We examined the effects of increasing intracellular cyclic AMP levels on the expression of human PAF receptor (hPAF-R). |
T3 |
217-293 |
Sentence |
denotes |
Peripheral blood monocytes constitutively expressed hPAF-R mRNA transcripts. |
T4 |
294-463 |
Sentence |
denotes |
A transiently elevated intracellular concentration of AMP induced with prostaglandin E2, cholera toxin, or forskolin was a sufficient signal to inhibit PAF-R expression. |
T5 |
464-680 |
Sentence |
denotes |
To determine the mechanisms of this inhibition, human monocytes were treated with dibutyryl cAMP, a cell-permeable cAMP analogue. cAMP reduced the expression of hPAF-R in a concentration- and a time-dependent manner. |
T6 |
681-750 |
Sentence |
denotes |
The effect was seen as early as 1 h and was essentially total by 4 h. |
T7 |
751-871 |
Sentence |
denotes |
Stability of hPAF-R mRNA was not markedly decreased by cAMP, as assessed by measuring the half-lives of the transcripts. |
T8 |
872-991 |
Sentence |
denotes |
Moreover, the nuclear transcription rate of the hPAF-R gene was reduced as early as 30 min after stimulation with cAMP. |
T9 |
992-1296 |
Sentence |
denotes |
The inhibition of hPAF-R mRNA accumulation was associated with diminished responsiveness to PAF, as assayed by intracellular Ca2+ fluxes, decreased number of binding sites, and decreased hPAF-R protein expression on the cell surface, as assessed by flow cytometry using a polyclonal anti-hPAF-R antibody. |
T10 |
1297-1455 |
Sentence |
denotes |
These data indicate that PAF-R expression can be regulated at the transcriptional and possibly post-transcriptional levels by elevation of intracellular cAMP. |