PubMed:8296666 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":99},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":100,"end":310},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":311,"end":442},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":443,"end":608},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":609,"end":747},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":99},"obj":"Sentence"},{"id":"T2","span":{"begin":100,"end":310},"obj":"Sentence"},{"id":"T3","span":{"begin":311,"end":442},"obj":"Sentence"},{"id":"T4","span":{"begin":443,"end":608},"obj":"Sentence"},{"id":"T5","span":{"begin":609,"end":747},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"A new approach to the molecular characterization of the c-ANCA antigen in Wegener's granulomatosis.\nClassic anti-neutrophil cytoplasmic antibodies (c-ANCA) specific for constituents of neutrophil primary granules and monocyte lysosomes have been implicated in the pathogenesis of Wegener's Granulomatosis (WG). The revised amino-terminal sequence of Proteinase 3 (PR-3) as ANCA antigen, suggested that PR-3 is identical to myeloblastin (MBN). As it has been proposed that autoantibodies recognize a conformational epitope on c-ANCA, prokaryotic expressed protein might not be recognized by the patients sera. Therefore we set up an in vitro translation in an eukaryotic cell-free system using an internal ATG (amino acid 15 of the mature protein)."}

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":56,"end":70},"obj":"gene:5657"},{"id":"T1","span":{"begin":84,"end":98},"obj":"disease:C0521173"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"A new approach to the molecular characterization of the c-ANCA antigen in Wegener's granulomatosis.\nClassic anti-neutrophil cytoplasmic antibodies (c-ANCA) specific for constituents of neutrophil primary granules and monocyte lysosomes have been implicated in the pathogenesis of Wegener's Granulomatosis (WG). The revised amino-terminal sequence of Proteinase 3 (PR-3) as ANCA antigen, suggested that PR-3 is identical to myeloblastin (MBN). As it has been proposed that autoantibodies recognize a conformational epitope on c-ANCA, prokaryotic expressed protein might not be recognized by the patients sera. Therefore we set up an in vitro translation in an eukaryotic cell-free system using an internal ATG (amino acid 15 of the mature protein)."}

{"project":"DisGeNet-2017-sample","denotations":[{"id":"T2206","span":{"begin":280,"end":304},"obj":"gene:474168"},{"id":"T2207","span":{"begin":306,"end":308},"obj":"disease:C3495801"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T2206","obj":"T2207"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"A new approach to the molecular characterization of the c-ANCA antigen in Wegener's granulomatosis.\nClassic anti-neutrophil cytoplasmic antibodies (c-ANCA) specific for constituents of neutrophil primary granules and monocyte lysosomes have been implicated in the pathogenesis of Wegener's Granulomatosis (WG). The revised amino-terminal sequence of Proteinase 3 (PR-3) as ANCA antigen, suggested that PR-3 is identical to myeloblastin (MBN). As it has been proposed that autoantibodies recognize a conformational epitope on c-ANCA, prokaryotic expressed protein might not be recognized by the patients sera. Therefore we set up an in vitro translation in an eukaryotic cell-free system using an internal ATG (amino acid 15 of the mature protein)."}