Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-98 |
Sentence |
denotes |
Energy-dependent protein-triacylglycerol interaction in a cell-free system from 3T3-L1 adipocytes. |
T2 |
99-288 |
Sentence |
denotes |
Triacylglycerol is synthesized from the precursors sn-1,2-diacylglycerol and and palmitoyl-CoA in a reaction catalyzed by the microsomal enzyme diacylglycerol acyltransferase (EC 2.3.1.20). |
T3 |
289-489 |
Sentence |
denotes |
Isolated 3T3-L1 adipocyte microsomal vesicles from cells pulse-labeled with L-[35S]methionine were found to release microsomal proteins into a low density form during the synthesis of triacylglycerol. |
T4 |
490-658 |
Sentence |
denotes |
The proteins released, which represent a subset of those present in the labeled microsomes, include a 62-kDa protein found in high concentration in mature fat droplets. |
T5 |
659-845 |
Sentence |
denotes |
The formation of the triacylglycerol-protein complexes was dependent on time and temperature, was not stimulated by cytosol, and required ATP as well as diacylglycerol and palmitoyl-CoA. |
T6 |
846-945 |
Sentence |
denotes |
Only nucleoside triphosphates and not non-hydrolyzable analogues could replace ATP in the reaction. |
T7 |
946-1124 |
Sentence |
denotes |
Unlike the enzyme reaction that measures the synthesis of triacylglycerol, the formation of low density membrane is thus dependent on ATP hydrolysis as well as enzyme substrates. |
T8 |
1125-1291 |
Sentence |
denotes |
The newly formed, low density particles are selectively enriched in triacylglycerol synthesized during the reaction as well as that synthesized prior to the reaction. |
T9 |
1292-1432 |
Sentence |
denotes |
The cell-free system described thus appears to represent an early adipogenic event leading to the lipid vacuoles found in mature adipocytes. |
T1 |
0-98 |
Sentence |
denotes |
Energy-dependent protein-triacylglycerol interaction in a cell-free system from 3T3-L1 adipocytes. |
T2 |
99-288 |
Sentence |
denotes |
Triacylglycerol is synthesized from the precursors sn-1,2-diacylglycerol and and palmitoyl-CoA in a reaction catalyzed by the microsomal enzyme diacylglycerol acyltransferase (EC 2.3.1.20). |
T3 |
289-489 |
Sentence |
denotes |
Isolated 3T3-L1 adipocyte microsomal vesicles from cells pulse-labeled with L-[35S]methionine were found to release microsomal proteins into a low density form during the synthesis of triacylglycerol. |
T4 |
490-658 |
Sentence |
denotes |
The proteins released, which represent a subset of those present in the labeled microsomes, include a 62-kDa protein found in high concentration in mature fat droplets. |
T5 |
659-845 |
Sentence |
denotes |
The formation of the triacylglycerol-protein complexes was dependent on time and temperature, was not stimulated by cytosol, and required ATP as well as diacylglycerol and palmitoyl-CoA. |
T6 |
846-945 |
Sentence |
denotes |
Only nucleoside triphosphates and not non-hydrolyzable analogues could replace ATP in the reaction. |
T7 |
946-1124 |
Sentence |
denotes |
Unlike the enzyme reaction that measures the synthesis of triacylglycerol, the formation of low density membrane is thus dependent on ATP hydrolysis as well as enzyme substrates. |
T8 |
1125-1291 |
Sentence |
denotes |
The newly formed, low density particles are selectively enriched in triacylglycerol synthesized during the reaction as well as that synthesized prior to the reaction. |
T9 |
1292-1432 |
Sentence |
denotes |
The cell-free system described thus appears to represent an early adipogenic event leading to the lipid vacuoles found in mature adipocytes. |