PubMed:8274409 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/8274409","sourcedb":"PubMed","sourceid":"8274409","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/8274409","text":"Androgen receptor gene mutations in human prostate cancer.\nTo investigate the structural abnormality of the androgen receptor (AR) in human prostate cancers, exons B-H encoding DNA- and hormone-binding domains were examined by single-strand conformation polymorphism analysis of polymerase chain reaction products using originally designed oligoprimers. Tissues from 7 cases of untreated stage B prostate cancer surgically removed and from 8 cases of endocrine therapy-resistant cancers obtained at autopsy were used in the study. Two different mutations were identified in exons D and H in the different cancer foci of the same cancer death patient. One mutation in exon D (at codon 701, Leu to His) was detected in the prostate, and the other in exon H (at codon 877, Thr to Ala) was found in metastatic tissues. In untreated cancer tissues and the other autopsy samples, no mutations were detected. The mutation in exon H was identical to that reported in LNCaP cells. These results indicate that AR gene mutations occur in relation to endocrine therapy-resistance, although the mutation was found in 1 out of 8 resistant cases (12.5%) at autopsy.","tracks":[{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":354,"end":361},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":806,"end":813},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":835,"end":842},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T2","pred":"source","obj":"performance-test"},{"subj":"PD-UBERON-AE-B_T3","pred":"source","obj":"performance-test"}]},{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":0,"end":17},"obj":"gene:367"},{"id":"T1","span":{"begin":42,"end":57},"obj":"disease:C0376358"},{"id":"T2","span":{"begin":0,"end":17},"obj":"gene:367"},{"id":"T3","span":{"begin":42,"end":57},"obj":"disease:C0600139"},{"id":"T4","span":{"begin":108,"end":125},"obj":"gene:367"},{"id":"T5","span":{"begin":140,"end":156},"obj":"disease:C0376358"},{"id":"T6","span":{"begin":127,"end":129},"obj":"gene:367"},{"id":"T7","span":{"begin":140,"end":156},"obj":"disease:C0376358"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"},{"subj":"T4","pred":"source","obj":"DisGeNET"},{"subj":"T5","pred":"source","obj":"DisGeNET"},{"subj":"T6","pred":"source","obj":"DisGeNET"},{"subj":"T7","pred":"source","obj":"DisGeNET"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":58},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":59,"end":353},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":354,"end":530},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":531,"end":650},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":651,"end":814},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":815,"end":901},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":902,"end":971},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":972,"end":1150},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":58},"obj":"Sentence"},{"id":"T2","span":{"begin":59,"end":353},"obj":"Sentence"},{"id":"T3","span":{"begin":354,"end":530},"obj":"Sentence"},{"id":"T4","span":{"begin":531,"end":650},"obj":"Sentence"},{"id":"T5","span":{"begin":651,"end":814},"obj":"Sentence"},{"id":"T6","span":{"begin":815,"end":901},"obj":"Sentence"},{"id":"T7","span":{"begin":902,"end":971},"obj":"Sentence"},{"id":"T8","span":{"begin":972,"end":1150},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"UBERON-AE","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":806,"end":813},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":835,"end":842},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"}],"attributes":[{"subj":"PD-UBERON-AE-B_T1","pred":"source","obj":"UBERON-AE"},{"subj":"PD-UBERON-AE-B_T2","pred":"source","obj":"UBERON-AE"}]},{"project":"DisGeNet-2017-sample","denotations":[{"id":"T947","span":{"begin":179,"end":307},"obj":"gene:367"},{"id":"T948","span":{"begin":642,"end":822},"obj":"disease:C0376358"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T947","obj":"T948"},{"id":"R2","pred":"associated_with","subj":"T947","obj":"T948"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T947","pred":"source","obj":"DisGeNet-2017-sample"},{"subj":"T948","pred":"source","obj":"DisGeNet-2017-sample"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"performance-test","color":"#ece293","default":true},{"id":"DisGeNET","color":"#c793ec"},{"id":"sentences","color":"#93ecad"},{"id":"UBERON-AE","color":"#ec9393"},{"id":"DisGeNet-2017-sample","color":"#93acec"}]}]}}