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PubMed:8227053 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-105 Sentence denotes Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells.
T2 106-278 Sentence denotes The biosynthesis of the prohormone convertase PC2 was studied in Chinese hamster ovary cells stably transfected with PC2 cDNA (CHO/PC2) and in rat insulinoma cells (Rin5f).
T3 279-494 Sentence denotes The major form of PC2 synthesized by CHO/PC2 cells was a 75-kDa protein corresponding to proPC2; this protein was retained intracellularly for 2-4 h following synthesis, suggesting prolonged intracellular residence.
T4 495-651 Sentence denotes In contrast, the major form of PC2 within Rin cells initially exhibited a molecular mass of 72 kDa and was then progressively converted to a 64-kDa species.
T5 652-763 Sentence denotes This 64-kDa species, which required 1-2 h to be released, was the major PC2 form detectable in Rin cell medium.
T6 764-1000 Sentence denotes Calcium-dependent benzyloxycarbonyl-Arg-Ser-Lys-Arg-aminomethylcoumarin cleaving activity was found in spent Rin cell medium; this activity could be immunoprecipitated with a carboxyl-terminal PC2 antibody, but not with preimmune serum.
T7 1001-1089 Sentence denotes In neither cell line did intracellular PC2 become endoglycosidase H-resistant over time.
T8 1090-1155 Sentence denotes PC2 released from Rin cells was also endoglycosidase H-sensitive.
T9 1156-1282 Sentence denotes Microsequencing and endoglycosidase H results indicate that 75-kDa CHO cell PC2 and 72-kDa Rin cell PC2 both represent proPC2.
T10 1283-1507 Sentence denotes We speculate that (a) PC2 undergoes unusual glycosylation, which may be related to its slow release from cells, and (b) the 64-kDa molecule detectable in spent Rin cell medium represents the enzymatically active form of PC2.
T1 0-105 Sentence denotes Biosynthesis of the prohormone convertase PC2 in Chinese hamster ovary cells and in rat insulinoma cells.
T2 106-278 Sentence denotes The biosynthesis of the prohormone convertase PC2 was studied in Chinese hamster ovary cells stably transfected with PC2 cDNA (CHO/PC2) and in rat insulinoma cells (Rin5f).
T3 279-494 Sentence denotes The major form of PC2 synthesized by CHO/PC2 cells was a 75-kDa protein corresponding to proPC2; this protein was retained intracellularly for 2-4 h following synthesis, suggesting prolonged intracellular residence.
T4 495-651 Sentence denotes In contrast, the major form of PC2 within Rin cells initially exhibited a molecular mass of 72 kDa and was then progressively converted to a 64-kDa species.
T5 652-763 Sentence denotes This 64-kDa species, which required 1-2 h to be released, was the major PC2 form detectable in Rin cell medium.
T6 764-1000 Sentence denotes Calcium-dependent benzyloxycarbonyl-Arg-Ser-Lys-Arg-aminomethylcoumarin cleaving activity was found in spent Rin cell medium; this activity could be immunoprecipitated with a carboxyl-terminal PC2 antibody, but not with preimmune serum.
T7 1001-1089 Sentence denotes In neither cell line did intracellular PC2 become endoglycosidase H-resistant over time.
T8 1090-1155 Sentence denotes PC2 released from Rin cells was also endoglycosidase H-sensitive.
T9 1156-1282 Sentence denotes Microsequencing and endoglycosidase H results indicate that 75-kDa CHO cell PC2 and 72-kDa Rin cell PC2 both represent proPC2.
T10 1283-1507 Sentence denotes We speculate that (a) PC2 undergoes unusual glycosylation, which may be related to its slow release from cells, and (b) the 64-kDa molecule detectable in spent Rin cell medium represents the enzymatically active form of PC2.

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 42-45 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T2 88-98 Disease denotes insulinoma http://purl.obolibrary.org/obo/MONDO_0024677
T3 152-155 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T4 223-226 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T5 237-240 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T6 253-263 Disease denotes insulinoma http://purl.obolibrary.org/obo/MONDO_0024677
T7 297-300 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T8 320-323 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T9 526-529 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T10 724-727 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T11 957-960 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T12 1040-1043 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T13 1090-1093 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T14 1232-1235 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T15 1256-1259 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T16 1305-1308 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174
T17 1503-1506 Disease denotes PC2 http://purl.obolibrary.org/obo/MONDO_0008174

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 88-98 Phenotype denotes insulinoma HP:0012197
T2 253-263 Phenotype denotes insulinoma HP:0012197

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 49-64 OrganismTaxon denotes Chinese hamster 10029
T2 84-87 OrganismTaxon denotes rat 10114|10116
T4 171-186 OrganismTaxon denotes Chinese hamster 10029
T5 249-252 OrganismTaxon denotes rat 10114|10116

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 65-70 Body_part denotes ovary http://purl.obolibrary.org/obo/UBERON_0000992
T2 187-192 Body_part denotes ovary http://purl.obolibrary.org/obo/UBERON_0000992
T3 402-417 Body_part denotes intracellularly http://purl.obolibrary.org/obo/GO_0005622
T4 470-483 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622
T5 1026-1039 Body_part denotes intracellular http://purl.obolibrary.org/obo/GO_0005622