PubMed:8119416 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/8119416","sourcedb":"PubMed","sourceid":"8119416","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/8119416","text":"Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli.\nThe yeast two hybrid system has been employed to identify cDNAs encoding proteins which interact with the gamma 1 isoform of human protein phosphatase 1. Here we report the isolation of cDNA encoding human protein phosphatase inhibitor. The deduced human sequence of 205 amino acids shows 92% identity to inhibitor 2 from rabbit. Human inhibitor 2 was expressed in E. coli and purified to homogeneity. The expressed human protein inhibited both native and bacterially expressed PP1, with the same Ki (1 nM) as inhibitor 2 purified from skeletal muscle. A gene or pseudogene for inhibitor 2 may be present near the major histocompatibility complex on chromosome 6.","tracks":[{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":48,"end":79},"obj":"protein"},{"id":"T2","span":{"begin":126,"end":137},"obj":"protein"},{"id":"T3","span":{"begin":281,"end":302},"obj":"protein"},{"id":"T4","span":{"begin":356,"end":385},"obj":"protein"},{"id":"T5","span":{"begin":455,"end":466},"obj":"protein"},{"id":"T6","span":{"begin":486,"end":497},"obj":"protein"},{"id":"T7","span":{"begin":660,"end":671},"obj":"protein"},{"id":"T8","span":{"begin":728,"end":739},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"AIMed"},{"subj":"T2","pred":"source","obj":"AIMed"},{"subj":"T3","pred":"source","obj":"AIMed"},{"subj":"T4","pred":"source","obj":"AIMed"},{"subj":"T5","pred":"source","obj":"AIMed"},{"subj":"T6","pred":"source","obj":"AIMed"},{"subj":"T7","pred":"source","obj":"AIMed"},{"subj":"T8","pred":"source","obj":"AIMed"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"AIMed","color":"#9793ec","default":true}]}]}}