PubMed:8119416 JSONTXT

{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":48,"end":79},"obj":"protein"},{"id":"T2","span":{"begin":126,"end":137},"obj":"protein"},{"id":"T3","span":{"begin":281,"end":302},"obj":"protein"},{"id":"T4","span":{"begin":356,"end":385},"obj":"protein"},{"id":"T5","span":{"begin":455,"end":466},"obj":"protein"},{"id":"T6","span":{"begin":486,"end":497},"obj":"protein"},{"id":"T7","span":{"begin":660,"end":671},"obj":"protein"},{"id":"T8","span":{"begin":728,"end":739},"obj":"protein"}],"text":"Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli.\nThe yeast two hybrid system has been employed to identify cDNAs encoding proteins which interact with the gamma 1 isoform of human protein phosphatase 1. Here we report the isolation of cDNA encoding human protein phosphatase inhibitor. The deduced human sequence of 205 amino acids shows 92% identity to inhibitor 2 from rabbit. Human inhibitor 2 was expressed in E. coli and purified to homogeneity. The expressed human protein inhibited both native and bacterially expressed PP1, with the same Ki (1 nM) as inhibitor 2 purified from skeletal muscle. A gene or pseudogene for inhibitor 2 may be present near the major histocompatibility complex on chromosome 6."}