PubMed:8054477 JSONTXT

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":992,"end":996},"obj":"gene:3558"},{"id":"T1","span":{"begin":1028,"end":1036},"obj":"disease:C0023418"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Positive and negative regulation of IL-2 gene expression: role of multiple regulatory sites.\nInterleukin 2 (IL-2) is an important lymphokine required in the process of T cell activation, proliferation, clonal expansion and differentiation. The IL-2 gene displays both T cell specific and inducible expression: it is only expressed in CD4+ T cells after antigenic or mitogenic stimulation. Several cis-acting regulatory sites are required for induction of the IL-2 gene after stimulation. In this study, we have analysed the function of these cis-acting regulatory sites in the context of the native IL-2 enhancer and promoter sequence. The results of this study suggest that the NFAT (-276 to -261), the distal octamer (-256 to -248) and the proximal octamer (-75 to -66) sites not only act as enhancers of IL-2 gene transcription in the presence of cellular stimulation, but also have a silencing effect on IL-2 gene expression in resting cells. Two other sites display disparate effects on IL-2 gene expression in different T leukemia cell lines: the distal purine box (-291 to -277) and the proximal purine box sites (-145 to -128). Finally, the AP-1 (-186 to -176) and the kappa B sites (-206 to -195) respond to different cellular activation in EL4 cells. The AP-1 site mediated the response to PMA stimulation while the kappa B site responded to IL-1 stimulation. These data suggest that the regulation of IL-2 gene expression is a complex process and multiple cis-acting regulatory sites interact to exert different effects in T cells representative of alternative stages of differentiation."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":36,"end":40},"obj":"protein"},{"id":"T2","span":{"begin":108,"end":112},"obj":"protein"},{"id":"T3","span":{"begin":130,"end":140},"obj":"protein"},{"id":"T4","span":{"begin":244,"end":253},"obj":"DNA"},{"id":"T5","span":{"begin":334,"end":346},"obj":"cell_type"},{"id":"T6","span":{"begin":397,"end":424},"obj":"DNA"},{"id":"T7","span":{"begin":459,"end":468},"obj":"DNA"},{"id":"T8","span":{"begin":542,"end":569},"obj":"DNA"},{"id":"T9","span":{"begin":599,"end":612},"obj":"DNA"},{"id":"T10","span":{"begin":617,"end":634},"obj":"DNA"},{"id":"T11","span":{"begin":679,"end":683},"obj":"protein"},{"id":"T12","span":{"begin":685,"end":697},"obj":"DNA"},{"id":"T13","span":{"begin":704,"end":718},"obj":"DNA"},{"id":"T14","span":{"begin":720,"end":732},"obj":"DNA"},{"id":"T15","span":{"begin":742,"end":758},"obj":"DNA"},{"id":"T16","span":{"begin":760,"end":770},"obj":"DNA"},{"id":"T17","span":{"begin":807,"end":816},"obj":"DNA"},{"id":"T18","span":{"begin":908,"end":917},"obj":"DNA"},{"id":"T19","span":{"begin":992,"end":1001},"obj":"DNA"},{"id":"T20","span":{"begin":1026,"end":1047},"obj":"cell_line"},{"id":"T21","span":{"begin":1053,"end":1070},"obj":"DNA"},{"id":"T22","span":{"begin":1072,"end":1084},"obj":"DNA"},{"id":"T23","span":{"begin":1094,"end":1119},"obj":"DNA"},{"id":"T24","span":{"begin":1121,"end":1133},"obj":"DNA"},{"id":"T25","span":{"begin":1149,"end":1190},"obj":"DNA"},{"id":"T26","span":{"begin":1192,"end":1204},"obj":"DNA"},{"id":"T27","span":{"begin":1250,"end":1259},"obj":"cell_line"},{"id":"T28","span":{"begin":1265,"end":1274},"obj":"DNA"},{"id":"T29","span":{"begin":1326,"end":1338},"obj":"DNA"},{"id":"T30","span":{"begin":1352,"end":1356},"obj":"protein"},{"id":"T31","span":{"begin":1412,"end":1421},"obj":"DNA"},{"id":"T32","span":{"begin":1467,"end":1494},"obj":"DNA"},{"id":"T33","span":{"begin":1534,"end":1541},"obj":"cell_type"}],"text":"Positive and negative regulation of IL-2 gene expression: role of multiple regulatory sites.\nInterleukin 2 (IL-2) is an important lymphokine required in the process of T cell activation, proliferation, clonal expansion and differentiation. The IL-2 gene displays both T cell specific and inducible expression: it is only expressed in CD4+ T cells after antigenic or mitogenic stimulation. Several cis-acting regulatory sites are required for induction of the IL-2 gene after stimulation. In this study, we have analysed the function of these cis-acting regulatory sites in the context of the native IL-2 enhancer and promoter sequence. The results of this study suggest that the NFAT (-276 to -261), the distal octamer (-256 to -248) and the proximal octamer (-75 to -66) sites not only act as enhancers of IL-2 gene transcription in the presence of cellular stimulation, but also have a silencing effect on IL-2 gene expression in resting cells. Two other sites display disparate effects on IL-2 gene expression in different T leukemia cell lines: the distal purine box (-291 to -277) and the proximal purine box sites (-145 to -128). Finally, the AP-1 (-186 to -176) and the kappa B sites (-206 to -195) respond to different cellular activation in EL4 cells. The AP-1 site mediated the response to PMA stimulation while the kappa B site responded to IL-1 stimulation. These data suggest that the regulation of IL-2 gene expression is a complex process and multiple cis-acting regulatory sites interact to exert different effects in T cells representative of alternative stages of differentiation."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"8054477-6#45#49#gene3558","span":{"begin":992,"end":996},"obj":"gene3558"},{"id":"8054477-6#81#89#diseaseC0023418","span":{"begin":1028,"end":1036},"obj":"diseaseC0023418"}],"relations":[{"id":"45#49#gene355881#89#diseaseC0023418","pred":"associated_with","subj":"8054477-6#45#49#gene3558","obj":"8054477-6#81#89#diseaseC0023418"}],"text":"Positive and negative regulation of IL-2 gene expression: role of multiple regulatory sites.\nInterleukin 2 (IL-2) is an important lymphokine required in the process of T cell activation, proliferation, clonal expansion and differentiation. The IL-2 gene displays both T cell specific and inducible expression: it is only expressed in CD4+ T cells after antigenic or mitogenic stimulation. Several cis-acting regulatory sites are required for induction of the IL-2 gene after stimulation. In this study, we have analysed the function of these cis-acting regulatory sites in the context of the native IL-2 enhancer and promoter sequence. The results of this study suggest that the NFAT (-276 to -261), the distal octamer (-256 to -248) and the proximal octamer (-75 to -66) sites not only act as enhancers of IL-2 gene transcription in the presence of cellular stimulation, but also have a silencing effect on IL-2 gene expression in resting cells. Two other sites display disparate effects on IL-2 gene expression in different T leukemia cell lines: the distal purine box (-291 to -277) and the proximal purine box sites (-145 to -128). Finally, the AP-1 (-186 to -176) and the kappa B sites (-206 to -195) respond to different cellular activation in EL4 cells. The AP-1 site mediated the response to PMA stimulation while the kappa B site responded to IL-1 stimulation. These data suggest that the regulation of IL-2 gene expression is a complex process and multiple cis-acting regulatory sites interact to exert different effects in T cells representative of alternative stages of differentiation."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":92},"obj":"Sentence"},{"id":"S2","span":{"begin":93,"end":239},"obj":"Sentence"},{"id":"S3","span":{"begin":240,"end":388},"obj":"Sentence"},{"id":"S4","span":{"begin":389,"end":487},"obj":"Sentence"},{"id":"S5","span":{"begin":488,"end":635},"obj":"Sentence"},{"id":"S6","span":{"begin":636,"end":946},"obj":"Sentence"},{"id":"S7","span":{"begin":947,"end":1135},"obj":"Sentence"},{"id":"S8","span":{"begin":1136,"end":1260},"obj":"Sentence"},{"id":"S9","span":{"begin":1261,"end":1369},"obj":"Sentence"},{"id":"S10","span":{"begin":1370,"end":1598},"obj":"Sentence"}],"text":"Positive and negative regulation of IL-2 gene expression: role of multiple regulatory sites.\nInterleukin 2 (IL-2) is an important lymphokine required in the process of T cell activation, proliferation, clonal expansion and differentiation. The IL-2 gene displays both T cell specific and inducible expression: it is only expressed in CD4+ T cells after antigenic or mitogenic stimulation. Several cis-acting regulatory sites are required for induction of the IL-2 gene after stimulation. In this study, we have analysed the function of these cis-acting regulatory sites in the context of the native IL-2 enhancer and promoter sequence. The results of this study suggest that the NFAT (-276 to -261), the distal octamer (-256 to -248) and the proximal octamer (-75 to -66) sites not only act as enhancers of IL-2 gene transcription in the presence of cellular stimulation, but also have a silencing effect on IL-2 gene expression in resting cells. Two other sites display disparate effects on IL-2 gene expression in different T leukemia cell lines: the distal purine box (-291 to -277) and the proximal purine box sites (-145 to -128). Finally, the AP-1 (-186 to -176) and the kappa B sites (-206 to -195) respond to different cellular activation in EL4 cells. The AP-1 site mediated the response to PMA stimulation while the kappa B site responded to IL-1 stimulation. These data suggest that the regulation of IL-2 gene expression is a complex process and multiple cis-acting regulatory sites interact to exert different effects in T cells representative of alternative stages of differentiation."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":36,"end":56},"obj":"NP"},{"id":"C2","span":{"begin":93,"end":113},"obj":"NP"},{"id":"C3","span":{"begin":240,"end":253},"obj":"NP"},{"id":"C4","span":{"begin":353,"end":387},"obj":"NP"},{"id":"C5","span":{"begin":389,"end":424},"obj":"NP"},{"id":"C6","span":{"begin":455,"end":468},"obj":"NP"},{"id":"C7","span":{"begin":475,"end":486},"obj":"NP"},{"id":"C8","span":{"begin":536,"end":569},"obj":"NP"},{"id":"C9","span":{"begin":908,"end":928},"obj":"NP"},{"id":"C10","span":{"begin":947,"end":962},"obj":"NP"},{"id":"C11","span":{"begin":992,"end":1012},"obj":"NP"},{"id":"C12","span":{"begin":1049,"end":1134},"obj":"NP"},{"id":"C13","span":{"begin":1412,"end":1432},"obj":"NP"},{"id":"C14","span":{"begin":1458,"end":1494},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C3","obj":"C2"},{"id":"R2","pred":"coref-ident","subj":"C6","obj":"C3"},{"id":"R3","pred":"coref-ident","subj":"C7","obj":"C4"},{"id":"R4","pred":"coref-ident","subj":"C8","obj":"C5"},{"id":"R5","pred":"coref-ident","subj":"C9","obj":"C1"},{"id":"R6","pred":"coref-ident","subj":"C11","obj":"C9"},{"id":"R7","pred":"coref-ident","subj":"C12","obj":"C10"},{"id":"R8","pred":"coref-ident","subj":"C13","obj":"C11"},{"id":"R9","pred":"coref-ident","subj":"C14","obj":"C5"}],"text":"Positive and negative regulation of IL-2 gene expression: role of multiple regulatory sites.\nInterleukin 2 (IL-2) is an important lymphokine required in the process of T cell activation, proliferation, clonal expansion and differentiation. The IL-2 gene displays both T cell specific and inducible expression: it is only expressed in CD4+ T cells after antigenic or mitogenic stimulation. Several cis-acting regulatory sites are required for induction of the IL-2 gene after stimulation. In this study, we have analysed the function of these cis-acting regulatory sites in the context of the native IL-2 enhancer and promoter sequence. The results of this study suggest that the NFAT (-276 to -261), the distal octamer (-256 to -248) and the proximal octamer (-75 to -66) sites not only act as enhancers of IL-2 gene transcription in the presence of cellular stimulation, but also have a silencing effect on IL-2 gene expression in resting cells. Two other sites display disparate effects on IL-2 gene expression in different T leukemia cell lines: the distal purine box (-291 to -277) and the proximal purine box sites (-145 to -128). Finally, the AP-1 (-186 to -176) and the kappa B sites (-206 to -195) respond to different cellular activation in EL4 cells. The AP-1 site mediated the response to PMA stimulation while the kappa B site responded to IL-1 stimulation. These data suggest that the regulation of IL-2 gene expression is a complex process and multiple cis-acting regulatory sites interact to exert different effects in T cells representative of alternative stages of differentiation."}

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