PubMed:8015552 JSONTXT

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{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":116},"obj":"Sentence"},{"id":"S2","span":{"begin":117,"end":268},"obj":"Sentence"},{"id":"S3","span":{"begin":269,"end":433},"obj":"Sentence"},{"id":"S4","span":{"begin":434,"end":672},"obj":"Sentence"},{"id":"S5","span":{"begin":673,"end":846},"obj":"Sentence"},{"id":"S6","span":{"begin":847,"end":1096},"obj":"Sentence"},{"id":"S7","span":{"begin":1097,"end":1303},"obj":"Sentence"},{"id":"S8","span":{"begin":1304,"end":1402},"obj":"Sentence"},{"id":"S9","span":{"begin":1403,"end":1511},"obj":"Sentence"},{"id":"S10","span":{"begin":1512,"end":1832},"obj":"Sentence"}],"text":"Multiple prolactin-responsive elements mediate G1 and S phase expression of the interferon regulatory factor-1 gene.\nThe interferon regulatory factor-1 (IRF-1) gene is both an immediate-early G1 phase gene and an S phase gene inducible by PRL in rat Nb2 T lymphocytes. To understand the mechanism by which PRL regulates the biphasic expression of IRF-1, we cloned the rat IRF-1 gene and functionally characterized the IRF-1 promoter. Upon transfection into Nb2 T cells, 1.7 kilobases (kb) of IRF-1 5'-flanking DNA linked to a chloramphenicol acetyl transferase (CAT) reporter gene mediated a 30-fold induction of CAT enzyme activity in response to 24 h of PRL stimulation. Deletion mutants containing 1.3, 0.6, and 0.2 kb 5'-flanking DNA were incrementally less transcriptionally active, although 0.2 kb still mediated a 12-fold induction by PRL. The sequence between -1.7 and -0.2 kb linked to a heterologous thymidine kinase promoter failed to respond to PRL stimulation, suggesting that the activity of upstream PRL response elements may require an interaction with promoter-proximal elements. By assaying CAT enzyme activity across a 24-h PRL induction time course, we were able to assign G1 vs. S phase PRL responses of the IRF-1 gene to different regions of the IRF-1 5'-flanking and promoter DNA. The 0.2-kb IRF-CAT construct was induced by PRL stimulation during the G1 phase of the cell cycle. In contrast, the 1.7-kb IRF-CAT construct was inducible by PRL during both G1 and S phase of the cell cycle. Hence, the PRL-induced biphasic expression of the IRF-1 gene appears to be controlled by separate PRL-responsive elements: elements in the first 0.2 kb of the IRF-1 promoter region act during early activation, and elements between 0.2 and 1.7 kb act in concert with the proximal 0.2-kb region during S phase progression."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":76,"end":115},"obj":"NP"},{"id":"C2","span":{"begin":117,"end":164},"obj":"NP"},{"id":"C3","span":{"begin":239,"end":242},"obj":"NP"},{"id":"C4","span":{"begin":246,"end":267},"obj":"NP"},{"id":"C5","span":{"begin":283,"end":296},"obj":"NP"},{"id":"C6","span":{"begin":300,"end":305},"obj":"NP"},{"id":"C7","span":{"begin":306,"end":309},"obj":"NP"},{"id":"C8","span":{"begin":347,"end":352},"obj":"NP"},{"id":"C9","span":{"begin":364,"end":382},"obj":"NP"},{"id":"C10","span":{"begin":457,"end":468},"obj":"NP"},{"id":"C11","span":{"begin":656,"end":671},"obj":"NP"},{"id":"C12","span":{"begin":842,"end":845},"obj":"NP"},{"id":"C13","span":{"begin":957,"end":972},"obj":"NP"},{"id":"C14","span":{"begin":1225,"end":1239},"obj":"NP"},{"id":"C15","span":{"begin":1348,"end":1363},"obj":"NP"},{"id":"C16","span":{"begin":1387,"end":1401},"obj":"NP"},{"id":"C17","span":{"begin":1462,"end":1465},"obj":"NP"},{"id":"C18","span":{"begin":1496,"end":1510},"obj":"NP"},{"id":"C19","span":{"begin":1523,"end":1527},"obj":"NP"},{"id":"C20","span":{"begin":1558,"end":1572},"obj":"NP"},{"id":"C21","span":{"begin":1610,"end":1614},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C2","obj":"C1"},{"id":"R2","pred":"coref-relat","subj":"C6","obj":"C5"},{"id":"R3","pred":"coref-ident","subj":"C7","obj":"C3"},{"id":"R4","pred":"coref-ident","subj":"C8","obj":"C2"},{"id":"R5","pred":"coref-ident","subj":"C9","obj":"C8"},{"id":"R6","pred":"coref-ident","subj":"C10","obj":"C4"},{"id":"R7","pred":"coref-ident","subj":"C12","obj":"C7"},{"id":"R8","pred":"coref-ident","subj":"C13","obj":"C11"},{"id":"R9","pred":"coref-ident","subj":"C14","obj":"C9"},{"id":"R10","pred":"coref-ident","subj":"C15","obj":"C13"},{"id":"R11","pred":"coref-ident","subj":"C17","obj":"C12"},{"id":"R12","pred":"coref-ident","subj":"C18","obj":"C16"},{"id":"R13","pred":"coref-ident","subj":"C19","obj":"C17"},{"id":"R14","pred":"coref-ident","subj":"C20","obj":"C14"},{"id":"R15","pred":"coref-ident","subj":"C21","obj":"C19"}],"text":"Multiple prolactin-responsive elements mediate G1 and S phase expression of the interferon regulatory factor-1 gene.\nThe interferon regulatory factor-1 (IRF-1) gene is both an immediate-early G1 phase gene and an S phase gene inducible by PRL in rat Nb2 T lymphocytes. To understand the mechanism by which PRL regulates the biphasic expression of IRF-1, we cloned the rat IRF-1 gene and functionally characterized the IRF-1 promoter. Upon transfection into Nb2 T cells, 1.7 kilobases (kb) of IRF-1 5'-flanking DNA linked to a chloramphenicol acetyl transferase (CAT) reporter gene mediated a 30-fold induction of CAT enzyme activity in response to 24 h of PRL stimulation. Deletion mutants containing 1.3, 0.6, and 0.2 kb 5'-flanking DNA were incrementally less transcriptionally active, although 0.2 kb still mediated a 12-fold induction by PRL. The sequence between -1.7 and -0.2 kb linked to a heterologous thymidine kinase promoter failed to respond to PRL stimulation, suggesting that the activity of upstream PRL response elements may require an interaction with promoter-proximal elements. By assaying CAT enzyme activity across a 24-h PRL induction time course, we were able to assign G1 vs. S phase PRL responses of the IRF-1 gene to different regions of the IRF-1 5'-flanking and promoter DNA. The 0.2-kb IRF-CAT construct was induced by PRL stimulation during the G1 phase of the cell cycle. In contrast, the 1.7-kb IRF-CAT construct was inducible by PRL during both G1 and S phase of the cell cycle. Hence, the PRL-induced biphasic expression of the IRF-1 gene appears to be controlled by separate PRL-responsive elements: elements in the first 0.2 kb of the IRF-1 promoter region act during early activation, and elements between 0.2 and 1.7 kb act in concert with the proximal 0.2-kb region during S phase progression."}

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