PubMed:7890777 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":169},"obj":"Sentence"},{"id":"T2","span":{"begin":170,"end":301},"obj":"Sentence"},{"id":"T3","span":{"begin":302,"end":431},"obj":"Sentence"},{"id":"T4","span":{"begin":432,"end":633},"obj":"Sentence"},{"id":"T5","span":{"begin":634,"end":751},"obj":"Sentence"},{"id":"T6","span":{"begin":752,"end":938},"obj":"Sentence"},{"id":"T7","span":{"begin":939,"end":1097},"obj":"Sentence"},{"id":"T8","span":{"begin":1098,"end":1181},"obj":"Sentence"},{"id":"T9","span":{"begin":1182,"end":1314},"obj":"Sentence"},{"id":"T10","span":{"begin":1315,"end":1453},"obj":"Sentence"},{"id":"T11","span":{"begin":1454,"end":1655},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":169},"obj":"Sentence"},{"id":"T2","span":{"begin":170,"end":301},"obj":"Sentence"},{"id":"T3","span":{"begin":302,"end":431},"obj":"Sentence"},{"id":"T4","span":{"begin":432,"end":633},"obj":"Sentence"},{"id":"T5","span":{"begin":634,"end":751},"obj":"Sentence"},{"id":"T6","span":{"begin":752,"end":938},"obj":"Sentence"},{"id":"T7","span":{"begin":939,"end":1097},"obj":"Sentence"},{"id":"T8","span":{"begin":1098,"end":1181},"obj":"Sentence"},{"id":"T9","span":{"begin":1182,"end":1314},"obj":"Sentence"},{"id":"T10","span":{"begin":1315,"end":1453},"obj":"Sentence"},{"id":"T11","span":{"begin":1454,"end":1655},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.\nWe have previously described a mutant B lymphoblastoid cell line, Clone-13, that expresses HLA-DQ in the absence of HLA-DR and -DP. Several criteria indicated that the defect in this cell line influences the activity of an isotype-specific transcription factor. Indeed, transient transfection of HLA-DRA and DQB reporter constructs indicated that the affected factor operates via cis-elements located between -141 base pairs and the transcription initiation site. A series of hybrid DRA/DQB reporter constructs was generated to further map the relevant cis-elements in this system. Insertion of oligonucleotides spanning the DQB X-box (but not the DQB-W region or the DQB Y-box) upstream of -141 in a DRA reporter plasmid rescued expression to nearly wild-type levels. Substitution promoters were then generated where the entire X-box, or only the X1- or X2-boxes of HLA-DRA were replaced with the analogous regions of HLA-DQB. The DQB X2-box was able to restore expression to the silent DRA reporter construct. Moreover, replacement of the DQB X2-box with the DRA X2-box markedly diminished the activity of the DQB promoter in the mutant cell. None of the hybrid reporter constructs were defective when transfected into the wild-type, HLA-DR/-DQ positive parental cell line, Jijoye. These studies suggest that the divergent X2-box of the class II major histocompatibility complex promoters plays an important role in influencing differential expression of the human class II isotypes."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":15,"end":31},"obj":"protein"},{"id":"T2","span":{"begin":39,"end":69},"obj":"protein"},{"id":"T3","span":{"begin":77,"end":144},"obj":"cell_line"},{"id":"T4","span":{"begin":152,"end":168},"obj":"DNA"},{"id":"T5","span":{"begin":201,"end":234},"obj":"cell_line"},{"id":"T6","span":{"begin":236,"end":245},"obj":"cell_line"},{"id":"T7","span":{"begin":261,"end":267},"obj":"protein"},{"id":"T8","span":{"begin":286,"end":300},"obj":"protein"},{"id":"T9","span":{"begin":393,"end":430},"obj":"protein"},{"id":"T10","span":{"begin":466,"end":501},"obj":"DNA"},{"id":"T11","span":{"begin":550,"end":562},"obj":"DNA"},{"id":"T12","span":{"begin":579,"end":594},"obj":"DNA"},{"id":"T13","span":{"begin":603,"end":632},"obj":"DNA"},{"id":"T14","span":{"begin":646,"end":680},"obj":"DNA"},{"id":"T15","span":{"begin":723,"end":735},"obj":"DNA"},{"id":"T16","span":{"begin":795,"end":804},"obj":"DNA"},{"id":"T17","span":{"begin":818,"end":830},"obj":"DNA"},{"id":"T18","span":{"begin":838,"end":847},"obj":"DNA"},{"id":"T19","span":{"begin":849,"end":865},"obj":"DNA"},{"id":"T20","span":{"begin":871,"end":891},"obj":"DNA"},{"id":"T21","span":{"begin":999,"end":1004},"obj":"DNA"},{"id":"T22","span":{"begin":1018,"end":1033},"obj":"DNA"},{"id":"T23","span":{"begin":1037,"end":1044},"obj":"DNA"},{"id":"T24","span":{"begin":1089,"end":1096},"obj":"DNA"},{"id":"T25","span":{"begin":1102,"end":1112},"obj":"DNA"},{"id":"T26","span":{"begin":1151,"end":1180},"obj":"DNA"},{"id":"T27","span":{"begin":1211,"end":1221},"obj":"DNA"},{"id":"T28","span":{"begin":1231,"end":1241},"obj":"DNA"},{"id":"T29","span":{"begin":1282,"end":1294},"obj":"DNA"},{"id":"T30","span":{"begin":1302,"end":1313},"obj":"cell_line"},{"id":"T31","span":{"begin":1327,"end":1353},"obj":"DNA"},{"id":"T32","span":{"begin":1395,"end":1404},"obj":"protein"},{"id":"T33","span":{"begin":1406,"end":1444},"obj":"cell_line"},{"id":"T34","span":{"begin":1446,"end":1452},"obj":"cell_line"},{"id":"T35","span":{"begin":1485,"end":1501},"obj":"DNA"},{"id":"T36","span":{"begin":1509,"end":1560},"obj":"DNA"},{"id":"T37","span":{"begin":1631,"end":1654},"obj":"DNA"}],"text":"Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.\nWe have previously described a mutant B lymphoblastoid cell line, Clone-13, that expresses HLA-DQ in the absence of HLA-DR and -DP. Several criteria indicated that the defect in this cell line influences the activity of an isotype-specific transcription factor. Indeed, transient transfection of HLA-DRA and DQB reporter constructs indicated that the affected factor operates via cis-elements located between -141 base pairs and the transcription initiation site. A series of hybrid DRA/DQB reporter constructs was generated to further map the relevant cis-elements in this system. Insertion of oligonucleotides spanning the DQB X-box (but not the DQB-W region or the DQB Y-box) upstream of -141 in a DRA reporter plasmid rescued expression to nearly wild-type levels. Substitution promoters were then generated where the entire X-box, or only the X1- or X2-boxes of HLA-DRA were replaced with the analogous regions of HLA-DQB. The DQB X2-box was able to restore expression to the silent DRA reporter construct. Moreover, replacement of the DQB X2-box with the DRA X2-box markedly diminished the activity of the DQB promoter in the mutant cell. None of the hybrid reporter constructs were defective when transfected into the wild-type, HLA-DR/-DQ positive parental cell line, Jijoye. These studies suggest that the divergent X2-box of the class II major histocompatibility complex promoters plays an important role in influencing differential expression of the human class II isotypes."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":73,"end":144},"obj":"NP"},{"id":"C2","span":{"begin":148,"end":168},"obj":"NP"},{"id":"C3","span":{"begin":199,"end":234},"obj":"NP"},{"id":"C4","span":{"begin":236,"end":244},"obj":"NP"},{"id":"C5","span":{"begin":246,"end":250},"obj":"NP"},{"id":"C6","span":{"begin":261,"end":267},"obj":"NP"},{"id":"C7","span":{"begin":348,"end":362},"obj":"NP"},{"id":"C8","span":{"begin":466,"end":501},"obj":"NP"},{"id":"C9","span":{"begin":634,"end":680},"obj":"NP"},{"id":"C10","span":{"begin":791,"end":804},"obj":"NP"},{"id":"C11","span":{"begin":988,"end":1004},"obj":"NP"},{"id":"C12","span":{"begin":1089,"end":1096},"obj":"NP"},{"id":"C13","span":{"begin":1098,"end":1112},"obj":"NP"},{"id":"C14","span":{"begin":1207,"end":1221},"obj":"NP"},{"id":"C15","span":{"begin":1323,"end":1353},"obj":"NP"},{"id":"C16","span":{"begin":1391,"end":1444},"obj":"NP"},{"id":"C17","span":{"begin":1446,"end":1452},"obj":"NP"},{"id":"C18","span":{"begin":1481,"end":1560},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-xxx","subj":"C3","obj":"C1"},{"id":"R2","pred":"coref-appos","subj":"C4","obj":"C3"},{"id":"R3","pred":"coref-relat","subj":"C5","obj":"C4"},{"id":"R4","pred":"coref-ident","subj":"C7","obj":"C3"},{"id":"R5","pred":"coref-ident","subj":"C9","obj":"C8"},{"id":"R6","pred":"coref-xxx","subj":"C11","obj":"C10"},{"id":"R7","pred":"coref-ident","subj":"C12","obj":"C6"},{"id":"R8","pred":"coref-ident","subj":"C14","obj":"C13"},{"id":"R9","pred":"coref-ident","subj":"C15","obj":"C9"},{"id":"R10","pred":"coref-appos","subj":"C17","obj":"C16"},{"id":"R11","pred":"coref-ident","subj":"C18","obj":"C2"}],"text":"Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.\nWe have previously described a mutant B lymphoblastoid cell line, Clone-13, that expresses HLA-DQ in the absence of HLA-DR and -DP. Several criteria indicated that the defect in this cell line influences the activity of an isotype-specific transcription factor. Indeed, transient transfection of HLA-DRA and DQB reporter constructs indicated that the affected factor operates via cis-elements located between -141 base pairs and the transcription initiation site. A series of hybrid DRA/DQB reporter constructs was generated to further map the relevant cis-elements in this system. Insertion of oligonucleotides spanning the DQB X-box (but not the DQB-W region or the DQB Y-box) upstream of -141 in a DRA reporter plasmid rescued expression to nearly wild-type levels. Substitution promoters were then generated where the entire X-box, or only the X1- or X2-boxes of HLA-DRA were replaced with the analogous regions of HLA-DQB. The DQB X2-box was able to restore expression to the silent DRA reporter construct. Moreover, replacement of the DQB X2-box with the DRA X2-box markedly diminished the activity of the DQB promoter in the mutant cell. None of the hybrid reporter constructs were defective when transfected into the wild-type, HLA-DR/-DQ positive parental cell line, Jijoye. These studies suggest that the divergent X2-box of the class II major histocompatibility complex promoters plays an important role in influencing differential expression of the human class II isotypes."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":169},"obj":"Sentence"},{"id":"S2","span":{"begin":170,"end":301},"obj":"Sentence"},{"id":"S3","span":{"begin":302,"end":431},"obj":"Sentence"},{"id":"S4","span":{"begin":432,"end":633},"obj":"Sentence"},{"id":"S5","span":{"begin":634,"end":751},"obj":"Sentence"},{"id":"S6","span":{"begin":752,"end":938},"obj":"Sentence"},{"id":"S7","span":{"begin":939,"end":1097},"obj":"Sentence"},{"id":"S8","span":{"begin":1098,"end":1181},"obj":"Sentence"},{"id":"S9","span":{"begin":1182,"end":1314},"obj":"Sentence"},{"id":"S10","span":{"begin":1315,"end":1453},"obj":"Sentence"},{"id":"S11","span":{"begin":1454,"end":1655},"obj":"Sentence"}],"text":"Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.\nWe have previously described a mutant B lymphoblastoid cell line, Clone-13, that expresses HLA-DQ in the absence of HLA-DR and -DP. Several criteria indicated that the defect in this cell line influences the activity of an isotype-specific transcription factor. Indeed, transient transfection of HLA-DRA and DQB reporter constructs indicated that the affected factor operates via cis-elements located between -141 base pairs and the transcription initiation site. A series of hybrid DRA/DQB reporter constructs was generated to further map the relevant cis-elements in this system. Insertion of oligonucleotides spanning the DQB X-box (but not the DQB-W region or the DQB Y-box) upstream of -141 in a DRA reporter plasmid rescued expression to nearly wild-type levels. Substitution promoters were then generated where the entire X-box, or only the X1- or X2-boxes of HLA-DRA were replaced with the analogous regions of HLA-DQB. The DQB X2-box was able to restore expression to the silent DRA reporter construct. Moreover, replacement of the DQB X2-box with the DRA X2-box markedly diminished the activity of the DQB promoter in the mutant cell. None of the hybrid reporter constructs were defective when transfected into the wild-type, HLA-DR/-DQ positive parental cell line, Jijoye. These studies suggest that the divergent X2-box of the class II major histocompatibility complex promoters plays an important role in influencing differential expression of the human class II isotypes."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":15,"end":31},"obj":"protein_domain_or_region"},{"id":"T2","span":{"begin":39,"end":48},"obj":"protein_family_or_group"},{"id":"T3","span":{"begin":49,"end":69},"obj":"protein_family_or_group"},{"id":"T4","span":{"begin":77,"end":144},"obj":"cell_line"},{"id":"T5","span":{"begin":152,"end":168},"obj":"DNA_domain_or_region"},{"id":"T6","span":{"begin":201,"end":234},"obj":"cell_line"},{"id":"T7","span":{"begin":236,"end":245},"obj":"cell_line"},{"id":"T8","span":{"begin":261,"end":267},"obj":"protein_molecule"},{"id":"T9","span":{"begin":393,"end":430},"obj":"protein_family_or_group"},{"id":"T10","span":{"begin":550,"end":562},"obj":"DNA_family_or_group"},{"id":"T11","span":{"begin":579,"end":594},"obj":"DNA_domain_or_region"},{"id":"T12","span":{"begin":603,"end":632},"obj":"DNA_domain_or_region"},{"id":"T13","span":{"begin":646,"end":660},"obj":"DNA_family_or_group"},{"id":"T14","span":{"begin":661,"end":680},"obj":"DNA_family_or_group"},{"id":"T15","span":{"begin":723,"end":735},"obj":"DNA_family_or_group"},{"id":"T16","span":{"begin":765,"end":781},"obj":"polynucleotide"},{"id":"T17","span":{"begin":795,"end":804},"obj":"DNA_domain_or_region"},{"id":"T18","span":{"begin":818,"end":830},"obj":"DNA_domain_or_region"},{"id":"T19","span":{"begin":838,"end":847},"obj":"DNA_domain_or_region"},{"id":"T20","span":{"begin":849,"end":865},"obj":"DNA_domain_or_region"},{"id":"T21","span":{"begin":871,"end":891},"obj":"DNA_molecule"},{"id":"T22","span":{"begin":921,"end":937},"obj":"other_name"},{"id":"T23","span":{"begin":999,"end":1004},"obj":"DNA_domain_or_region"},{"id":"T24","span":{"begin":1037,"end":1044},"obj":"DNA_domain_or_region"},{"id":"T25","span":{"begin":1089,"end":1096},"obj":"DNA_domain_or_region"},{"id":"T26","span":{"begin":1102,"end":1112},"obj":"DNA_domain_or_region"},{"id":"T27","span":{"begin":1151,"end":1180},"obj":"DNA_molecule"},{"id":"T28","span":{"begin":1211,"end":1221},"obj":"DNA_domain_or_region"},{"id":"T29","span":{"begin":1231,"end":1241},"obj":"DNA_domain_or_region"},{"id":"T30","span":{"begin":1282,"end":1294},"obj":"DNA_domain_or_region"},{"id":"T31","span":{"begin":1302,"end":1313},"obj":"cell_line"},{"id":"T32","span":{"begin":1327,"end":1333},"obj":"DNA_family_or_group"},{"id":"T33","span":{"begin":1334,"end":1353},"obj":"DNA_family_or_group"},{"id":"T34","span":{"begin":1395,"end":1404},"obj":"protein_family_or_group"},{"id":"T35","span":{"begin":1406,"end":1444},"obj":"cell_line"},{"id":"T36","span":{"begin":1446,"end":1452},"obj":"cell_line"},{"id":"T37","span":{"begin":1485,"end":1501},"obj":"DNA_domain_or_region"},{"id":"T38","span":{"begin":1509,"end":1550},"obj":"protein_complex"},{"id":"T39","span":{"begin":1600,"end":1623},"obj":"other_name"},{"id":"T40","span":{"begin":1631,"end":1654},"obj":"DNA_family_or_group"}],"text":"Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.\nWe have previously described a mutant B lymphoblastoid cell line, Clone-13, that expresses HLA-DQ in the absence of HLA-DR and -DP. Several criteria indicated that the defect in this cell line influences the activity of an isotype-specific transcription factor. Indeed, transient transfection of HLA-DRA and DQB reporter constructs indicated that the affected factor operates via cis-elements located between -141 base pairs and the transcription initiation site. A series of hybrid DRA/DQB reporter constructs was generated to further map the relevant cis-elements in this system. Insertion of oligonucleotides spanning the DQB X-box (but not the DQB-W region or the DQB Y-box) upstream of -141 in a DRA reporter plasmid rescued expression to nearly wild-type levels. Substitution promoters were then generated where the entire X-box, or only the X1- or X2-boxes of HLA-DRA were replaced with the analogous regions of HLA-DQB. The DQB X2-box was able to restore expression to the silent DRA reporter construct. Moreover, replacement of the DQB X2-box with the DRA X2-box markedly diminished the activity of the DQB promoter in the mutant cell. None of the hybrid reporter constructs were defective when transfected into the wild-type, HLA-DR/-DQ positive parental cell line, Jijoye. These studies suggest that the divergent X2-box of the class II major histocompatibility complex promoters plays an important role in influencing differential expression of the human class II isotypes."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":470,"end":473},"obj":"Disease"},{"id":"T2","span":{"begin":653,"end":656},"obj":"Disease"},{"id":"T3","span":{"begin":871,"end":874},"obj":"Disease"},{"id":"T4","span":{"begin":1041,"end":1044},"obj":"Disease"},{"id":"T5","span":{"begin":1158,"end":1161},"obj":"Disease"},{"id":"T6","span":{"begin":1231,"end":1234},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0019440"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0019440"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0019440"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MONDO_0019440"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MONDO_0019440"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MONDO_0019440"}],"text":"Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.\nWe have previously described a mutant B lymphoblastoid cell line, Clone-13, that expresses HLA-DQ in the absence of HLA-DR and -DP. Several criteria indicated that the defect in this cell line influences the activity of an isotype-specific transcription factor. Indeed, transient transfection of HLA-DRA and DQB reporter constructs indicated that the affected factor operates via cis-elements located between -141 base pairs and the transcription initiation site. A series of hybrid DRA/DQB reporter constructs was generated to further map the relevant cis-elements in this system. Insertion of oligonucleotides spanning the DQB X-box (but not the DQB-W region or the DQB Y-box) upstream of -141 in a DRA reporter plasmid rescued expression to nearly wild-type levels. Substitution promoters were then generated where the entire X-box, or only the X1- or X2-boxes of HLA-DRA were replaced with the analogous regions of HLA-DQB. The DQB X2-box was able to restore expression to the silent DRA reporter construct. Moreover, replacement of the DQB X2-box with the DRA X2-box markedly diminished the activity of the DQB promoter in the mutant cell. None of the hybrid reporter constructs were defective when transfected into the wild-type, HLA-DR/-DQ positive parental cell line, Jijoye. These studies suggest that the divergent X2-box of the class II major histocompatibility complex promoters plays an important role in influencing differential expression of the human class II isotypes."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":1631,"end":1636},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"}],"text":"Mapping of the interaction site of the defective transcription factor in the class II major histocompatibility complex mutant cell line clone-13 to the divergent X2-box.\nWe have previously described a mutant B lymphoblastoid cell line, Clone-13, that expresses HLA-DQ in the absence of HLA-DR and -DP. Several criteria indicated that the defect in this cell line influences the activity of an isotype-specific transcription factor. Indeed, transient transfection of HLA-DRA and DQB reporter constructs indicated that the affected factor operates via cis-elements located between -141 base pairs and the transcription initiation site. A series of hybrid DRA/DQB reporter constructs was generated to further map the relevant cis-elements in this system. Insertion of oligonucleotides spanning the DQB X-box (but not the DQB-W region or the DQB Y-box) upstream of -141 in a DRA reporter plasmid rescued expression to nearly wild-type levels. Substitution promoters were then generated where the entire X-box, or only the X1- or X2-boxes of HLA-DRA were replaced with the analogous regions of HLA-DQB. The DQB X2-box was able to restore expression to the silent DRA reporter construct. Moreover, replacement of the DQB X2-box with the DRA X2-box markedly diminished the activity of the DQB promoter in the mutant cell. None of the hybrid reporter constructs were defective when transfected into the wild-type, HLA-DR/-DQ positive parental cell line, Jijoye. These studies suggest that the divergent X2-box of the class II major histocompatibility complex promoters plays an important role in influencing differential expression of the human class II isotypes."}