PubMed:7826623 JSONTXT

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":0,"end":25},"obj":"DNA"},{"id":"T2","span":{"begin":33,"end":61},"obj":"DNA"},{"id":"T3","span":{"begin":67,"end":98},"obj":"DNA"},{"id":"T4","span":{"begin":144,"end":151},"obj":"cell_type"},{"id":"T5","span":{"begin":284,"end":298},"obj":"cell_line"},{"id":"T6","span":{"begin":304,"end":321},"obj":"protein"},{"id":"T7","span":{"begin":323,"end":326},"obj":"protein"},{"id":"T8","span":{"begin":400,"end":409},"obj":"DNA"},{"id":"T9","span":{"begin":565,"end":603},"obj":"DNA"},{"id":"T10","span":{"begin":610,"end":651},"obj":"DNA"},{"id":"T11","span":{"begin":675,"end":702},"obj":"DNA"},{"id":"T12","span":{"begin":710,"end":719},"obj":"DNA"},{"id":"T13","span":{"begin":725,"end":740},"obj":"DNA"},{"id":"T14","span":{"begin":749,"end":761},"obj":"DNA"},{"id":"T15","span":{"begin":786,"end":805},"obj":"DNA"},{"id":"T16","span":{"begin":811,"end":831},"obj":"protein"},{"id":"T17","span":{"begin":832,"end":836},"obj":"protein"},{"id":"T18","span":{"begin":936,"end":959},"obj":"DNA"},{"id":"T19","span":{"begin":985,"end":999},"obj":"DNA"},{"id":"T20","span":{"begin":1093,"end":1114},"obj":"DNA"},{"id":"T21","span":{"begin":1145,"end":1149},"obj":"protein"}],"text":"T-cell functional regions of the human IL-3 proximal promoter.\nThe human interleukin-3 (IL-3) gene is expressed almost exclusively in activated T cells. Its expression is regulated at both the transcriptional and post-transcriptional level. We have previously shown that treatment of Jurkat T cells with phytohemaglutinin (PHA) and the phorbol ester, PMA, activated transcription initiation from the IL-3 gene. To define the regions of the gene required for transcription activation, we generated a series of reporter constructs containing different regions of the IL-3 gene 5' and 3' flanking sequences. Both positive and negative regulatory elements were identified in the proximal 5' flanking region of the IL-3 gene. The promoter region between -173 and -60 contained the strongest activating elements. The transcription factor AP-1 could bind to this positive activator region of the promoter. We also examined the function of the IL-3 CK-1/CK-2 elements that are present in many cytokine genes and found that they acted as a repressor of basal level expression when cloned upstream of a heterologous promoter but were also inducible by PMA/PHA."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":62},"obj":"Sentence"},{"id":"S2","span":{"begin":63,"end":152},"obj":"Sentence"},{"id":"S3","span":{"begin":153,"end":240},"obj":"Sentence"},{"id":"S4","span":{"begin":241,"end":410},"obj":"Sentence"},{"id":"S5","span":{"begin":411,"end":604},"obj":"Sentence"},{"id":"S6","span":{"begin":605,"end":720},"obj":"Sentence"},{"id":"S7","span":{"begin":721,"end":806},"obj":"Sentence"},{"id":"S8","span":{"begin":807,"end":898},"obj":"Sentence"},{"id":"S9","span":{"begin":899,"end":1150},"obj":"Sentence"}],"text":"T-cell functional regions of the human IL-3 proximal promoter.\nThe human interleukin-3 (IL-3) gene is expressed almost exclusively in activated T cells. Its expression is regulated at both the transcriptional and post-transcriptional level. We have previously shown that treatment of Jurkat T cells with phytohemaglutinin (PHA) and the phorbol ester, PMA, activated transcription initiation from the IL-3 gene. To define the regions of the gene required for transcription activation, we generated a series of reporter constructs containing different regions of the IL-3 gene 5' and 3' flanking sequences. Both positive and negative regulatory elements were identified in the proximal 5' flanking region of the IL-3 gene. The promoter region between -173 and -60 contained the strongest activating elements. The transcription factor AP-1 could bind to this positive activator region of the promoter. We also examined the function of the IL-3 CK-1/CK-2 elements that are present in many cytokine genes and found that they acted as a repressor of basal level expression when cloned upstream of a heterologous promoter but were also inducible by PMA/PHA."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":63,"end":98},"obj":"NP"},{"id":"C2","span":{"begin":153,"end":156},"obj":"NP"},{"id":"C4","span":{"begin":332,"end":349},"obj":"NP"},{"id":"C5","span":{"begin":351,"end":354},"obj":"NP"},{"id":"C3","span":{"begin":304,"end":354},"obj":"NP"},{"id":"C6","span":{"begin":396,"end":409},"obj":"NP"},{"id":"C7","span":{"begin":436,"end":444},"obj":"NP"},{"id":"C8","span":{"begin":706,"end":719},"obj":"NP"},{"id":"C9","span":{"begin":721,"end":761},"obj":"NP"},{"id":"C10","span":{"begin":851,"end":897},"obj":"NP"},{"id":"C11","span":{"begin":932,"end":959},"obj":"NP"},{"id":"C12","span":{"begin":960,"end":964},"obj":"NP"},{"id":"C13","span":{"begin":1015,"end":1019},"obj":"NP"},{"id":"C14","span":{"begin":1142,"end":1149},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-pron","subj":"C2","obj":"C1"},{"id":"R2","pred":"coref-appos","subj":"C5","obj":"C4"},{"id":"R3","pred":"coref-ident","subj":"C6","obj":"C1"},{"id":"R4","pred":"coref-ident","subj":"C7","obj":"C6"},{"id":"R5","pred":"coref-ident","subj":"C8","obj":"C6"},{"id":"R6","pred":"coref-ident","subj":"C10","obj":"C9"},{"id":"R7","pred":"coref-relat","subj":"C12","obj":"C11"},{"id":"R8","pred":"coref-pron","subj":"C13","obj":"C11"},{"id":"R9","pred":"coref-ident","subj":"C14","obj":"C3"}],"text":"T-cell functional regions of the human IL-3 proximal promoter.\nThe human interleukin-3 (IL-3) gene is expressed almost exclusively in activated T cells. Its expression is regulated at both the transcriptional and post-transcriptional level. We have previously shown that treatment of Jurkat T cells with phytohemaglutinin (PHA) and the phorbol ester, PMA, activated transcription initiation from the IL-3 gene. To define the regions of the gene required for transcription activation, we generated a series of reporter constructs containing different regions of the IL-3 gene 5' and 3' flanking sequences. Both positive and negative regulatory elements were identified in the proximal 5' flanking region of the IL-3 gene. The promoter region between -173 and -60 contained the strongest activating elements. The transcription factor AP-1 could bind to this positive activator region of the promoter. We also examined the function of the IL-3 CK-1/CK-2 elements that are present in many cytokine genes and found that they acted as a repressor of basal level expression when cloned upstream of a heterologous promoter but were also inducible by PMA/PHA."}

{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":25},"obj":"DNA_domain_or_region"},{"id":"T2","span":{"begin":33,"end":61},"obj":"DNA_domain_or_region"},{"id":"T3","span":{"begin":67,"end":88},"obj":"DNA_domain_or_region"},{"id":"T4","span":{"begin":88,"end":92},"obj":"protein_molecule"},{"id":"T5","span":{"begin":144,"end":151},"obj":"cell_type"},{"id":"T6","span":{"begin":284,"end":290},"obj":"cell_line"},{"id":"T7","span":{"begin":291,"end":298},"obj":"cell_type"},{"id":"T8","span":{"begin":304,"end":321},"obj":"protein_molecule"},{"id":"T9","span":{"begin":323,"end":326},"obj":"protein_molecule"},{"id":"T10","span":{"begin":336,"end":349},"obj":"other_organic_compound"},{"id":"T11","span":{"begin":351,"end":354},"obj":"other_organic_compound"},{"id":"T12","span":{"begin":400,"end":409},"obj":"DNA_domain_or_region"},{"id":"T13","span":{"begin":565,"end":574},"obj":"DNA_domain_or_region"},{"id":"T14","span":{"begin":675,"end":702},"obj":"DNA_domain_or_region"},{"id":"T15","span":{"begin":710,"end":719},"obj":"DNA_domain_or_region"},{"id":"T16","span":{"begin":725,"end":740},"obj":"DNA_domain_or_region"},{"id":"T17","span":{"begin":749,"end":761},"obj":"DNA_domain_or_region"},{"id":"T18","span":{"begin":786,"end":805},"obj":"DNA_domain_or_region"},{"id":"T19","span":{"begin":811,"end":831},"obj":"protein_family_or_group"},{"id":"T20","span":{"begin":832,"end":836},"obj":"protein_molecule"},{"id":"T21","span":{"begin":936,"end":959},"obj":"DNA_domain_or_region"},{"id":"T22","span":{"begin":985,"end":999},"obj":"DNA_family_or_group"},{"id":"T23","span":{"begin":1044,"end":1066},"obj":"other_name"},{"id":"T24","span":{"begin":1093,"end":1114},"obj":"DNA_domain_or_region"},{"id":"T25","span":{"begin":1142,"end":1145},"obj":"other_organic_compound"},{"id":"T26","span":{"begin":1145,"end":1149},"obj":"protein_molecule"}],"text":"T-cell functional regions of the human IL-3 proximal promoter.\nThe human interleukin-3 (IL-3) gene is expressed almost exclusively in activated T cells. Its expression is regulated at both the transcriptional and post-transcriptional level. We have previously shown that treatment of Jurkat T cells with phytohemaglutinin (PHA) and the phorbol ester, PMA, activated transcription initiation from the IL-3 gene. To define the regions of the gene required for transcription activation, we generated a series of reporter constructs containing different regions of the IL-3 gene 5' and 3' flanking sequences. Both positive and negative regulatory elements were identified in the proximal 5' flanking region of the IL-3 gene. The promoter region between -173 and -60 contained the strongest activating elements. The transcription factor AP-1 could bind to this positive activator region of the promoter. We also examined the function of the IL-3 CK-1/CK-2 elements that are present in many cytokine genes and found that they acted as a repressor of basal level expression when cloned upstream of a heterologous promoter but were also inducible by PMA/PHA."}