PubMed:7826623 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T1	0-25	DNA	denotes	T-cell functional regions
T2	33-61	DNA	denotes	human IL-3 proximal promoter
T3	67-98	DNA	denotes	human interleukin-3 (IL-3) gene
T4	144-151	cell_type	denotes	T cells
T5	284-298	cell_line	denotes	Jurkat T cells
T6	304-321	protein	denotes	phytohemaglutinin
T7	323-326	protein	denotes	PHA
T8	400-409	DNA	denotes	IL-3 gene
T9	565-603	DNA	denotes	IL-3 gene 5' and 3' flanking sequences
T10	610-651	DNA	denotes	positive and negative regulatory elements
T11	675-702	DNA	denotes	proximal 5' flanking region
T12	710-719	DNA	denotes	IL-3 gene
T13	725-740	DNA	denotes	promoter region
T14	749-761	DNA	denotes	-173 and -60
T15	786-805	DNA	denotes	activating elements
T16	811-831	protein	denotes	transcription factor
T17	832-836	protein	denotes	AP-1
T18	936-959	DNA	denotes	IL-3 CK-1/CK-2 elements
T19	985-999	DNA	denotes	cytokine genes
T20	1093-1114	DNA	denotes	heterologous promoter
T21	1145-1149	protein	denotes	/PHA

Id	Subject	Object	Predicate	Lexical cue
S1	0-62	Sentence	denotes	T-cell functional regions of the human IL-3 proximal promoter.
S2	63-152	Sentence	denotes	The human interleukin-3 (IL-3) gene is expressed almost exclusively in activated T cells.
S3	153-240	Sentence	denotes	Its expression is regulated at both the transcriptional and post-transcriptional level.
S4	241-410	Sentence	denotes	We have previously shown that treatment of Jurkat T cells with phytohemaglutinin (PHA) and the phorbol ester, PMA, activated transcription initiation from the IL-3 gene.
S5	411-604	Sentence	denotes	To define the regions of the gene required for transcription activation, we generated a series of reporter constructs containing different regions of the IL-3 gene 5' and 3' flanking sequences.
S6	605-720	Sentence	denotes	Both positive and negative regulatory elements were identified in the proximal 5' flanking region of the IL-3 gene.
S7	721-806	Sentence	denotes	The promoter region between -173 and -60 contained the strongest activating elements.
S8	807-898	Sentence	denotes	The transcription factor AP-1 could bind to this positive activator region of the promoter.
S9	899-1150	Sentence	denotes	We also examined the function of the IL-3 CK-1/CK-2 elements that are present in many cytokine genes and found that they acted as a repressor of basal level expression when cloned upstream of a heterologous promoter but were also inducible by PMA/PHA.

Id	Subject	Object	Predicate	Lexical cue
C1	63-98	NP	denotes	The human interleukin-3 (IL-3) gene
C2	153-156	NP	denotes	Its
C4	332-349	NP	denotes	the phorbol ester
C5	351-354	NP	denotes	PMA
C3	304-354	NP	denotes	phytohemaglutinin (PHA) and the phorbol ester, PMA
C6	396-409	NP	denotes	the IL-3 gene
C7	436-444	NP	denotes	the gene
C8	706-719	NP	denotes	the IL-3 gene
C9	721-761	NP	denotes	The promoter region between -173 and -60
C10	851-897	NP	denotes	this positive activator region of the promoter
C11	932-959	NP	denotes	the IL-3 CK-1/CK-2 elements
C12	960-964	NP	denotes	that
C13	1015-1019	NP	denotes	they
C14	1142-1149	NP	denotes	PMA/PHA
R1	C2	C1	coref-pron	Its,The human interleukin-3 (IL-3) gene
R2	C5	C4	coref-appos	PMA,the phorbol ester
R3	C6	C1	coref-ident	the IL-3 gene,The human interleukin-3 (IL-3) gene
R4	C7	C6	coref-ident	the gene,the IL-3 gene
R5	C8	C6	coref-ident	the IL-3 gene,the IL-3 gene
R6	C10	C9	coref-ident	this positive activator region of the promoter,The promoter region between -173 and -60
R7	C12	C11	coref-relat	that,the IL-3 CK-1/CK-2 elements
R8	C13	C11	coref-pron	they,the IL-3 CK-1/CK-2 elements
R9	C14	C3	coref-ident	PMA/PHA,"phytohemaglutinin (PHA) and the phorbol ester, PMA"

Id	Subject	Object	Predicate	Lexical cue
T1	0-25	DNA_domain_or_region	denotes	T-cell functional regions
T2	33-61	DNA_domain_or_region	denotes	human IL-3 proximal promoter
T3	67-88	DNA_domain_or_region	denotes	human interleukin-3 (
T4	88-92	protein_molecule	denotes	IL-3
T5	144-151	cell_type	denotes	T cells
T6	284-290	cell_line	denotes	Jurkat
T7	291-298	cell_type	denotes	T cells
T8	304-321	protein_molecule	denotes	phytohemaglutinin
T9	323-326	protein_molecule	denotes	PHA
T10	336-349	other_organic_compound	denotes	phorbol ester
T11	351-354	other_organic_compound	denotes	PMA
T12	400-409	DNA_domain_or_region	denotes	IL-3 gene
T13	565-574	DNA_domain_or_region	denotes	IL-3 gene
T14	675-702	DNA_domain_or_region	denotes	proximal 5' flanking region
T15	710-719	DNA_domain_or_region	denotes	IL-3 gene
T16	725-740	DNA_domain_or_region	denotes	promoter region
T17	749-761	DNA_domain_or_region	denotes	-173 and -60
T18	786-805	DNA_domain_or_region	denotes	activating elements
T19	811-831	protein_family_or_group	denotes	transcription factor
T20	832-836	protein_molecule	denotes	AP-1
T21	936-959	DNA_domain_or_region	denotes	IL-3 CK-1/CK-2 elements
T22	985-999	DNA_family_or_group	denotes	cytokine genes
T23	1044-1066	other_name	denotes	basal level expression
T24	1093-1114	DNA_domain_or_region	denotes	heterologous promoter
T25	1142-1145	other_organic_compound	denotes	PMA
T26	1145-1149	protein_molecule	denotes	/PHA