PubMed:7769834 JSONTXT

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":14,"end":21},"obj":"protein"},{"id":"T2","span":{"begin":26,"end":54},"obj":"DNA"},{"id":"T3","span":{"begin":69,"end":85},"obj":"protein"},{"id":"T4","span":{"begin":89,"end":102},"obj":"cell_type"},{"id":"T5","span":{"begin":153,"end":169},"obj":"protein"},{"id":"T6","span":{"begin":171,"end":174},"obj":"protein"},{"id":"T7","span":{"begin":196,"end":224},"obj":"DNA"},{"id":"T8","span":{"begin":226,"end":231},"obj":"DNA"},{"id":"T9","span":{"begin":236,"end":256},"obj":"cell_type"},{"id":"T10","span":{"begin":258,"end":261},"obj":"protein"},{"id":"T11","span":{"begin":272,"end":277},"obj":"DNA"},{"id":"T12","span":{"begin":390,"end":414},"obj":"DNA"},{"id":"T13","span":{"begin":429,"end":473},"obj":"DNA"},{"id":"T14","span":{"begin":492,"end":495},"obj":"protein"},{"id":"T15","span":{"begin":504,"end":509},"obj":"DNA"},{"id":"T16","span":{"begin":544,"end":554},"obj":"DNA"},{"id":"T17","span":{"begin":556,"end":566},"obj":"DNA"},{"id":"T18","span":{"begin":575,"end":589},"obj":"DNA"},{"id":"T19","span":{"begin":642,"end":651},"obj":"protein"},{"id":"T20","span":{"begin":653,"end":660},"obj":"protein"},{"id":"T21","span":{"begin":695,"end":697},"obj":"protein"},{"id":"T22","span":{"begin":743,"end":762},"obj":"cell_line"},{"id":"T23","span":{"begin":808,"end":811},"obj":"protein"},{"id":"T24","span":{"begin":875,"end":880},"obj":"DNA"},{"id":"T25","span":{"begin":894,"end":914},"obj":"cell_type"}],"text":"Activation of pp90rsk and early growth response-1 gene expression by pokeweed mitogen in human B cells.\nThe present studies have examined the effects of pokeweed mitogen (PWM) on the induction of early growth response-1 gene (EGR-1) in normal human B cells. PWM regulates EGR-1 gene expression by both transcriptional and post-transcriptional mechanisms. Transient transfection assays with EGR-1 promoter fragments linked to the chloramphenicol acetyltransferase (CAT) gene demonstrated that PWM induced EGR-1 transcription is conferred by the CArG motif (C C[AT]6GG) in the EGR-1 promoter. The results further demonstrated the activation of S6 kinase (pp90rsk), evidenced by phosphorylation of S6 and serum response factor (SRF) peptides, in PWM treated B cells. Taken together, these findings suggest that PWM is able to initiate an intracytoplasmic signalling cascade and EGR-1 induction in normal human B cells."}

{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":103},"obj":"Sentence"},{"id":"S2","span":{"begin":104,"end":257},"obj":"Sentence"},{"id":"S3","span":{"begin":258,"end":354},"obj":"Sentence"},{"id":"S4","span":{"begin":355,"end":590},"obj":"Sentence"},{"id":"S5","span":{"begin":591,"end":763},"obj":"Sentence"},{"id":"S6","span":{"begin":764,"end":915},"obj":"Sentence"}],"text":"Activation of pp90rsk and early growth response-1 gene expression by pokeweed mitogen in human B cells.\nThe present studies have examined the effects of pokeweed mitogen (PWM) on the induction of early growth response-1 gene (EGR-1) in normal human B cells. PWM regulates EGR-1 gene expression by both transcriptional and post-transcriptional mechanisms. Transient transfection assays with EGR-1 promoter fragments linked to the chloramphenicol acetyltransferase (CAT) gene demonstrated that PWM induced EGR-1 transcription is conferred by the CArG motif (C C[AT]6GG) in the EGR-1 promoter. The results further demonstrated the activation of S6 kinase (pp90rsk), evidenced by phosphorylation of S6 and serum response factor (SRF) peptides, in PWM treated B cells. Taken together, these findings suggest that PWM is able to initiate an intracytoplasmic signalling cascade and EGR-1 induction in normal human B cells."}

{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":0,"end":21},"obj":"NP"},{"id":"C2","span":{"begin":26,"end":65},"obj":"NP"},{"id":"C3","span":{"begin":69,"end":85},"obj":"NP"},{"id":"C4","span":{"begin":153,"end":175},"obj":"NP"},{"id":"C5","span":{"begin":179,"end":232},"obj":"NP"},{"id":"C6","span":{"begin":236,"end":256},"obj":"NP"},{"id":"C7","span":{"begin":258,"end":261},"obj":"NP"},{"id":"C8","span":{"begin":624,"end":661},"obj":"NP"},{"id":"C9","span":{"begin":808,"end":811},"obj":"NP"},{"id":"C10","span":{"begin":875,"end":890},"obj":"NP"},{"id":"C11","span":{"begin":894,"end":914},"obj":"NP"}],"relations":[{"id":"R1","pred":"coref-ident","subj":"C4","obj":"C3"},{"id":"R2","pred":"coref-ident","subj":"C5","obj":"C2"},{"id":"R3","pred":"coref-ident","subj":"C7","obj":"C4"},{"id":"R4","pred":"coref-ident","subj":"C8","obj":"C1"},{"id":"R5","pred":"coref-ident","subj":"C9","obj":"C7"},{"id":"R6","pred":"coref-ident","subj":"C10","obj":"C5"},{"id":"R7","pred":"coref-ident","subj":"C11","obj":"C6"}],"text":"Activation of pp90rsk and early growth response-1 gene expression by pokeweed mitogen in human B cells.\nThe present studies have examined the effects of pokeweed mitogen (PWM) on the induction of early growth response-1 gene (EGR-1) in normal human B cells. PWM regulates EGR-1 gene expression by both transcriptional and post-transcriptional mechanisms. Transient transfection assays with EGR-1 promoter fragments linked to the chloramphenicol acetyltransferase (CAT) gene demonstrated that PWM induced EGR-1 transcription is conferred by the CArG motif (C C[AT]6GG) in the EGR-1 promoter. The results further demonstrated the activation of S6 kinase (pp90rsk), evidenced by phosphorylation of S6 and serum response factor (SRF) peptides, in PWM treated B cells. Taken together, these findings suggest that PWM is able to initiate an intracytoplasmic signalling cascade and EGR-1 induction in normal human B cells."}

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