PubMed:7532282
Annnotations
jnlpba-st-training
{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":38,"end":53},"obj":"protein"},{"id":"T2","span":{"begin":135,"end":161},"obj":"RNA"},{"id":"T3","span":{"begin":184,"end":205},"obj":"protein"},{"id":"T4","span":{"begin":212,"end":232},"obj":"DNA"},{"id":"T5","span":{"begin":236,"end":251},"obj":"cell_type"},{"id":"T6","span":{"begin":430,"end":453},"obj":"protein"},{"id":"T7","span":{"begin":591,"end":614},"obj":"protein"},{"id":"T8","span":{"begin":648,"end":688},"obj":"protein"},{"id":"T9","span":{"begin":744,"end":754},"obj":"protein"},{"id":"T10","span":{"begin":768,"end":794},"obj":"DNA"},{"id":"T11","span":{"begin":879,"end":905},"obj":"RNA"},{"id":"T12","span":{"begin":979,"end":989},"obj":"protein"},{"id":"T13","span":{"begin":1035,"end":1061},"obj":"DNA"},{"id":"T14","span":{"begin":1126,"end":1156},"obj":"DNA"},{"id":"T15","span":{"begin":1170,"end":1205},"obj":"DNA"},{"id":"T16","span":{"begin":1278,"end":1304},"obj":"DNA"},{"id":"T17","span":{"begin":1374,"end":1400},"obj":"RNA"},{"id":"T18","span":{"begin":1438,"end":1442},"obj":"RNA"},{"id":"T19","span":{"begin":1579,"end":1597},"obj":"cell_type"},{"id":"T20","span":{"begin":1621,"end":1635},"obj":"RNA"},{"id":"T21","span":{"begin":1680,"end":1706},"obj":"RNA"},{"id":"T22","span":{"begin":1790,"end":1826},"obj":"RNA"},{"id":"T23","span":{"begin":1851,"end":1869},"obj":"cell_type"},{"id":"T24","span":{"begin":1912,"end":1938},"obj":"RNA"},{"id":"T25","span":{"begin":1991,"end":2017},"obj":"DNA"},{"id":"T26","span":{"begin":2106,"end":2132},"obj":"RNA"},{"id":"T27","span":{"begin":2153,"end":2179},"obj":"DNA"},{"id":"T28","span":{"begin":2237,"end":2263},"obj":"RNA"}],"text":"Transcription-independent turnover of I kappa B alpha during monocyte adherence: implications for a translational component regulating I kappa B alpha/MAD-3 mRNA levels.\nWe identified I kappa B alpha/MAD-3 as an immediate-early gene in human monocytes that is expressed in response to a variety of signals, including adhesion, lipopolysaccharide, and phorbol myristate acetate. Within 5 min of monocyte adhesion, the level of the I kappa B alpha protein is markedly diminished but is rapidly replaced in a cycloheximide-sensitive manner within 20 min. Accompanying the rapid turnover of the I kappa B alpha protein is simultaneous translocation of NF-kappa B-related transcription factors to nuclei of adhered monocytes. The demonstration that NF-kappa B can regulate I kappa B alpha/MAD-3 gene transcription in other cell types suggested that the rapid increase in steady-state I kappa B alpha/MAD-3 mRNA levels we observed within 30 min of monocyte adherence would result from NF-kappa B-dependent transcriptional stimulation of the I kappa B alpha/MAD-3 gene. Nuclear run-on analyses indicated that, instead, while several immediate-early cytokine genes, such as the interleukin 1 beta (IL-1 beta) gene, were transcriptionally activated during monocyte adhesion, the rate of I kappa B alpha/MAD-3 gene transcription remained constant. The adherence-dependent increase in I kappa B alpha/MAD-3 mRNA levels was also not a consequence of mRNA stabilization events. Interestingly, while increases in both IL-1 beta and I kappa B alpha/MAD-3 mRNA levels were detected in nuclei of adherent monocytes, cytoplasmic levels of IL-1 beta mRNA increased during adherence whereas those of I kappa B alpha/MAD-3 mRNA did not. Taken together, our data suggest that two interactive mechanisms regulate monocytic I kappa B alpha/MAD-3 mRNA levels. We propose that adherent monocytes regulate nuclear processing (or decay) of I kappa B alpha/MAD-3 mRNA, thereby increasing mRNA levels without stimulating I kappa B alpha/MAD-3 gene transcription. Moreover, since inhibition of protein synthesis leads to accumulation of I kappa B alpha/MAD-3 mRNA without stimulating I kappa B alpha/MAD-3 gene transcription, we suggest that low cytoplasmic levels of I kappa B alpha/MAD-3 mRNA are maintained by a translation-dependent degradation mechanism."}
genia-medco-coref
{"project":"genia-medco-coref","denotations":[{"id":"C1","span":{"begin":61,"end":79},"obj":"NP"},{"id":"C2","span":{"begin":135,"end":168},"obj":"NP"},{"id":"C3","span":{"begin":184,"end":205},"obj":"NP"},{"id":"C4","span":{"begin":209,"end":251},"obj":"NP"},{"id":"C5","span":{"begin":252,"end":256},"obj":"NP"},{"id":"C6","span":{"begin":394,"end":411},"obj":"NP"},{"id":"C7","span":{"begin":426,"end":453},"obj":"NP"},{"id":"C8","span":{"begin":587,"end":614},"obj":"NP"},{"id":"C9","span":{"begin":692,"end":719},"obj":"NP"},{"id":"C10","span":{"begin":768,"end":808},"obj":"NP"},{"id":"C11","span":{"begin":879,"end":912},"obj":"NP"},{"id":"C12","span":{"begin":942,"end":960},"obj":"NP"},{"id":"C13","span":{"begin":1031,"end":1061},"obj":"NP"},{"id":"C14","span":{"begin":1247,"end":1264},"obj":"NP"},{"id":"C15","span":{"begin":1278,"end":1318},"obj":"NP"},{"id":"C16","span":{"begin":1374,"end":1407},"obj":"NP"},{"id":"C17","span":{"begin":1569,"end":1597},"obj":"NP"},{"id":"C18","span":{"begin":1599,"end":1617},"obj":"NP"},{"id":"C19","span":{"begin":1671,"end":1676},"obj":"NP"},{"id":"C20","span":{"begin":1680,"end":1706},"obj":"NP"},{"id":"C21","span":{"begin":1912,"end":1938},"obj":"NP"},{"id":"C22","span":{"begin":1991,"end":2031},"obj":"NP"},{"id":"C23","span":{"begin":2106,"end":2132},"obj":"NP"},{"id":"C24","span":{"begin":2153,"end":2193},"obj":"NP"},{"id":"C25","span":{"begin":2237,"end":2263},"obj":"NP"}],"relations":[{"id":"R8","pred":"coref-ident","subj":"C15","obj":"C10"},{"id":"R9","pred":"coref-ident","subj":"C16","obj":"C11"},{"id":"R10","pred":"coref-ident","subj":"C17","obj":"C9"},{"id":"R1","pred":"coref-relat","subj":"C5","obj":"C4"},{"id":"R2","pred":"coref-ident","subj":"C6","obj":"C1"},{"id":"R3","pred":"coref-ident","subj":"C8","obj":"C7"},{"id":"R4","pred":"coref-ident","subj":"C11","obj":"C2"},{"id":"R5","pred":"coref-ident","subj":"C12","obj":"C1"},{"id":"R6","pred":"coref-ident","subj":"C13","obj":"C3"},{"id":"R7","pred":"coref-ident","subj":"C14","obj":"C6"},{"id":"R11","pred":"coref-pron","subj":"C19","obj":"C18"},{"id":"R12","pred":"coref-ident","subj":"C21","obj":"C20"},{"id":"R13","pred":"coref-ident","subj":"C22","obj":"C15"},{"id":"R14","pred":"coref-ident","subj":"C23","obj":"C21"},{"id":"R15","pred":"coref-ident","subj":"C24","obj":"C22"},{"id":"R16","pred":"coref-ident","subj":"C25","obj":"C23"}],"text":"Transcription-independent turnover of I kappa B alpha during monocyte adherence: implications for a translational component regulating I kappa B alpha/MAD-3 mRNA levels.\nWe identified I kappa B alpha/MAD-3 as an immediate-early gene in human monocytes that is expressed in response to a variety of signals, including adhesion, lipopolysaccharide, and phorbol myristate acetate. Within 5 min of monocyte adhesion, the level of the I kappa B alpha protein is markedly diminished but is rapidly replaced in a cycloheximide-sensitive manner within 20 min. Accompanying the rapid turnover of the I kappa B alpha protein is simultaneous translocation of NF-kappa B-related transcription factors to nuclei of adhered monocytes. The demonstration that NF-kappa B can regulate I kappa B alpha/MAD-3 gene transcription in other cell types suggested that the rapid increase in steady-state I kappa B alpha/MAD-3 mRNA levels we observed within 30 min of monocyte adherence would result from NF-kappa B-dependent transcriptional stimulation of the I kappa B alpha/MAD-3 gene. Nuclear run-on analyses indicated that, instead, while several immediate-early cytokine genes, such as the interleukin 1 beta (IL-1 beta) gene, were transcriptionally activated during monocyte adhesion, the rate of I kappa B alpha/MAD-3 gene transcription remained constant. The adherence-dependent increase in I kappa B alpha/MAD-3 mRNA levels was also not a consequence of mRNA stabilization events. Interestingly, while increases in both IL-1 beta and I kappa B alpha/MAD-3 mRNA levels were detected in nuclei of adherent monocytes, cytoplasmic levels of IL-1 beta mRNA increased during adherence whereas those of I kappa B alpha/MAD-3 mRNA did not. Taken together, our data suggest that two interactive mechanisms regulate monocytic I kappa B alpha/MAD-3 mRNA levels. We propose that adherent monocytes regulate nuclear processing (or decay) of I kappa B alpha/MAD-3 mRNA, thereby increasing mRNA levels without stimulating I kappa B alpha/MAD-3 gene transcription. Moreover, since inhibition of protein synthesis leads to accumulation of I kappa B alpha/MAD-3 mRNA without stimulating I kappa B alpha/MAD-3 gene transcription, we suggest that low cytoplasmic levels of I kappa B alpha/MAD-3 mRNA are maintained by a translation-dependent degradation mechanism."}
pubmed-sentences-benchmark
{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":169},"obj":"Sentence"},{"id":"S2","span":{"begin":170,"end":377},"obj":"Sentence"},{"id":"S3","span":{"begin":378,"end":551},"obj":"Sentence"},{"id":"S4","span":{"begin":552,"end":720},"obj":"Sentence"},{"id":"S5","span":{"begin":721,"end":1062},"obj":"Sentence"},{"id":"S6","span":{"begin":1063,"end":1337},"obj":"Sentence"},{"id":"S7","span":{"begin":1338,"end":1464},"obj":"Sentence"},{"id":"S8","span":{"begin":1465,"end":1715},"obj":"Sentence"},{"id":"S9","span":{"begin":1716,"end":1834},"obj":"Sentence"},{"id":"S10","span":{"begin":1835,"end":2032},"obj":"Sentence"},{"id":"S11","span":{"begin":2033,"end":2328},"obj":"Sentence"}],"text":"Transcription-independent turnover of I kappa B alpha during monocyte adherence: implications for a translational component regulating I kappa B alpha/MAD-3 mRNA levels.\nWe identified I kappa B alpha/MAD-3 as an immediate-early gene in human monocytes that is expressed in response to a variety of signals, including adhesion, lipopolysaccharide, and phorbol myristate acetate. Within 5 min of monocyte adhesion, the level of the I kappa B alpha protein is markedly diminished but is rapidly replaced in a cycloheximide-sensitive manner within 20 min. Accompanying the rapid turnover of the I kappa B alpha protein is simultaneous translocation of NF-kappa B-related transcription factors to nuclei of adhered monocytes. The demonstration that NF-kappa B can regulate I kappa B alpha/MAD-3 gene transcription in other cell types suggested that the rapid increase in steady-state I kappa B alpha/MAD-3 mRNA levels we observed within 30 min of monocyte adherence would result from NF-kappa B-dependent transcriptional stimulation of the I kappa B alpha/MAD-3 gene. Nuclear run-on analyses indicated that, instead, while several immediate-early cytokine genes, such as the interleukin 1 beta (IL-1 beta) gene, were transcriptionally activated during monocyte adhesion, the rate of I kappa B alpha/MAD-3 gene transcription remained constant. The adherence-dependent increase in I kappa B alpha/MAD-3 mRNA levels was also not a consequence of mRNA stabilization events. Interestingly, while increases in both IL-1 beta and I kappa B alpha/MAD-3 mRNA levels were detected in nuclei of adherent monocytes, cytoplasmic levels of IL-1 beta mRNA increased during adherence whereas those of I kappa B alpha/MAD-3 mRNA did not. Taken together, our data suggest that two interactive mechanisms regulate monocytic I kappa B alpha/MAD-3 mRNA levels. We propose that adherent monocytes regulate nuclear processing (or decay) of I kappa B alpha/MAD-3 mRNA, thereby increasing mRNA levels without stimulating I kappa B alpha/MAD-3 gene transcription. Moreover, since inhibition of protein synthesis leads to accumulation of I kappa B alpha/MAD-3 mRNA without stimulating I kappa B alpha/MAD-3 gene transcription, we suggest that low cytoplasmic levels of I kappa B alpha/MAD-3 mRNA are maintained by a translation-dependent degradation mechanism."}
GENIAcorpus
{"project":"GENIAcorpus","denotations":[{"id":"T1","span":{"begin":0,"end":34},"obj":"other_name"},{"id":"T2","span":{"begin":38,"end":53},"obj":"protein_molecule"},{"id":"T3","span":{"begin":61,"end":79},"obj":"other_name"},{"id":"T4","span":{"begin":135,"end":150},"obj":"protein_molecule"},{"id":"T5","span":{"begin":150,"end":156},"obj":"protein_molecule"},{"id":"T6","span":{"begin":184,"end":199},"obj":"protein_molecule"},{"id":"T7","span":{"begin":212,"end":232},"obj":"DNA_domain_or_region"},{"id":"T8","span":{"begin":236,"end":251},"obj":"cell_type"},{"id":"T9","span":{"begin":317,"end":325},"obj":"other_name"},{"id":"T10","span":{"begin":327,"end":345},"obj":"lipid"},{"id":"T11","span":{"begin":351,"end":376},"obj":"other_organic_compound"},{"id":"T12","span":{"begin":394,"end":411},"obj":"other_name"},{"id":"T13","span":{"begin":430,"end":445},"obj":"protein_molecule"},{"id":"T14","span":{"begin":506,"end":536},"obj":"other_name"},{"id":"T15","span":{"begin":591,"end":606},"obj":"protein_molecule"},{"id":"T16","span":{"begin":648,"end":666},"obj":"protein_family_or_group"},{"id":"T17","span":{"begin":667,"end":688},"obj":"protein_family_or_group"},{"id":"T18","span":{"begin":744,"end":754},"obj":"protein_molecule"},{"id":"T19","span":{"begin":768,"end":783},"obj":"protein_molecule"},{"id":"T20","span":{"begin":866,"end":878},"obj":"other_name"},{"id":"T21","span":{"begin":879,"end":894},"obj":"protein_molecule"},{"id":"T22","span":{"begin":942,"end":960},"obj":"other_name"},{"id":"T23","span":{"begin":979,"end":989},"obj":"protein_molecule"},{"id":"T24","span":{"begin":1035,"end":1050},"obj":"protein_molecule"},{"id":"T25","span":{"begin":1126,"end":1156},"obj":"DNA_family_or_group"},{"id":"T26","span":{"begin":1170,"end":1188},"obj":"protein_molecule"},{"id":"T27","span":{"begin":1190,"end":1199},"obj":"protein_molecule"},{"id":"T28","span":{"begin":1247,"end":1264},"obj":"other_name"},{"id":"T29","span":{"begin":1278,"end":1293},"obj":"protein_molecule"},{"id":"T30","span":{"begin":1374,"end":1389},"obj":"protein_molecule"},{"id":"T31","span":{"begin":1438,"end":1442},"obj":"RNA_family_or_group"},{"id":"T32","span":{"begin":1569,"end":1575},"obj":"cell_component"},{"id":"T33","span":{"begin":1579,"end":1597},"obj":"cell_type"},{"id":"T34","span":{"begin":1599,"end":1617},"obj":"other_name"},{"id":"T35","span":{"begin":1621,"end":1630},"obj":"protein_molecule"},{"id":"T36","span":{"begin":1680,"end":1695},"obj":"protein_molecule"},{"id":"T37","span":{"begin":1790,"end":1799},"obj":"RNA_molecule"},{"id":"T38","span":{"begin":1800,"end":1815},"obj":"protein_molecule"},{"id":"T39","span":{"begin":1851,"end":1869},"obj":"cell_type"},{"id":"T40","span":{"begin":1912,"end":1927},"obj":"protein_molecule"},{"id":"T41","span":{"begin":1991,"end":2006},"obj":"protein_molecule"},{"id":"T42","span":{"begin":2106,"end":2121},"obj":"protein_molecule"},{"id":"T43","span":{"begin":2153,"end":2168},"obj":"protein_molecule"},{"id":"T44","span":{"begin":2215,"end":2233},"obj":"other_name"},{"id":"T45","span":{"begin":2237,"end":2252},"obj":"protein_molecule"},{"id":"T46","span":{"begin":2284,"end":2327},"obj":"other_name"}],"text":"Transcription-independent turnover of I kappa B alpha during monocyte adherence: implications for a translational component regulating I kappa B alpha/MAD-3 mRNA levels.\nWe identified I kappa B alpha/MAD-3 as an immediate-early gene in human monocytes that is expressed in response to a variety of signals, including adhesion, lipopolysaccharide, and phorbol myristate acetate. Within 5 min of monocyte adhesion, the level of the I kappa B alpha protein is markedly diminished but is rapidly replaced in a cycloheximide-sensitive manner within 20 min. Accompanying the rapid turnover of the I kappa B alpha protein is simultaneous translocation of NF-kappa B-related transcription factors to nuclei of adhered monocytes. The demonstration that NF-kappa B can regulate I kappa B alpha/MAD-3 gene transcription in other cell types suggested that the rapid increase in steady-state I kappa B alpha/MAD-3 mRNA levels we observed within 30 min of monocyte adherence would result from NF-kappa B-dependent transcriptional stimulation of the I kappa B alpha/MAD-3 gene. Nuclear run-on analyses indicated that, instead, while several immediate-early cytokine genes, such as the interleukin 1 beta (IL-1 beta) gene, were transcriptionally activated during monocyte adhesion, the rate of I kappa B alpha/MAD-3 gene transcription remained constant. The adherence-dependent increase in I kappa B alpha/MAD-3 mRNA levels was also not a consequence of mRNA stabilization events. Interestingly, while increases in both IL-1 beta and I kappa B alpha/MAD-3 mRNA levels were detected in nuclei of adherent monocytes, cytoplasmic levels of IL-1 beta mRNA increased during adherence whereas those of I kappa B alpha/MAD-3 mRNA did not. Taken together, our data suggest that two interactive mechanisms regulate monocytic I kappa B alpha/MAD-3 mRNA levels. We propose that adherent monocytes regulate nuclear processing (or decay) of I kappa B alpha/MAD-3 mRNA, thereby increasing mRNA levels without stimulating I kappa B alpha/MAD-3 gene transcription. Moreover, since inhibition of protein synthesis leads to accumulation of I kappa B alpha/MAD-3 mRNA without stimulating I kappa B alpha/MAD-3 gene transcription, we suggest that low cytoplasmic levels of I kappa B alpha/MAD-3 mRNA are maintained by a translation-dependent degradation mechanism."}