PubMed:7499267 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":217},"obj":"Sentence"},{"id":"T2","span":{"begin":218,"end":493},"obj":"Sentence"},{"id":"T3","span":{"begin":494,"end":698},"obj":"Sentence"},{"id":"T4","span":{"begin":699,"end":731},"obj":"Sentence"},{"id":"T5","span":{"begin":732,"end":1029},"obj":"Sentence"},{"id":"T6","span":{"begin":1030,"end":1115},"obj":"Sentence"},{"id":"T7","span":{"begin":1116,"end":1387},"obj":"Sentence"},{"id":"T8","span":{"begin":1388,"end":1536},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":217},"obj":"Sentence"},{"id":"T2","span":{"begin":218,"end":493},"obj":"Sentence"},{"id":"T3","span":{"begin":494,"end":698},"obj":"Sentence"},{"id":"T4","span":{"begin":699,"end":731},"obj":"Sentence"},{"id":"T5","span":{"begin":732,"end":1029},"obj":"Sentence"},{"id":"T6","span":{"begin":1030,"end":1115},"obj":"Sentence"},{"id":"T7","span":{"begin":1116,"end":1387},"obj":"Sentence"},{"id":"T8","span":{"begin":1388,"end":1536},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.\nWe investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma."}

{"project":"jnlpba-st-training","denotations":[{"id":"T1","span":{"begin":143,"end":173},"obj":"protein"},{"id":"T2","span":{"begin":175,"end":185},"obj":"protein"},{"id":"T3","span":{"begin":191,"end":194},"obj":"protein"},{"id":"T4","span":{"begin":195,"end":216},"obj":"protein"},{"id":"T5","span":{"begin":286,"end":302},"obj":"protein"},{"id":"T6","span":{"begin":304,"end":313},"obj":"protein"},{"id":"T7","span":{"begin":315,"end":342},"obj":"protein"},{"id":"T8","span":{"begin":344,"end":353},"obj":"protein"},{"id":"T9","span":{"begin":358,"end":389},"obj":"DNA"},{"id":"T10","span":{"begin":393,"end":414},"obj":"cell_type"},{"id":"T11","span":{"begin":518,"end":531},"obj":"DNA"},{"id":"T12","span":{"begin":587,"end":596},"obj":"protein"},{"id":"T13","span":{"begin":604,"end":613},"obj":"protein"},{"id":"T14","span":{"begin":778,"end":793},"obj":"DNA"},{"id":"T15","span":{"begin":814,"end":823},"obj":"protein"},{"id":"T16","span":{"begin":912,"end":921},"obj":"protein"},{"id":"T17","span":{"begin":926,"end":935},"obj":"protein"},{"id":"T18","span":{"begin":957,"end":980},"obj":"DNA"},{"id":"T19","span":{"begin":1013,"end":1022},"obj":"protein"},{"id":"T20","span":{"begin":1065,"end":1075},"obj":"protein"},{"id":"T21","span":{"begin":1094,"end":1114},"obj":"protein"},{"id":"T22","span":{"begin":1145,"end":1154},"obj":"DNA"},{"id":"T23","span":{"begin":1158,"end":1167},"obj":"protein"},{"id":"T24","span":{"begin":1172,"end":1181},"obj":"protein"},{"id":"T25","span":{"begin":1186,"end":1201},"obj":"cell_type"},{"id":"T26","span":{"begin":1236,"end":1241},"obj":"protein"},{"id":"T27","span":{"begin":1246,"end":1271},"obj":"protein"},{"id":"T28","span":{"begin":1327,"end":1357},"obj":"DNA"},{"id":"T29","span":{"begin":1373,"end":1386},"obj":"DNA"},{"id":"T30","span":{"begin":1431,"end":1454},"obj":"protein"},{"id":"T31","span":{"begin":1526,"end":1535},"obj":"protein"}],"text":"Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.\nWe investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma."}

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{"project":"pubmed-sentences-benchmark","denotations":[{"id":"S1","span":{"begin":0,"end":217},"obj":"Sentence"},{"id":"S2","span":{"begin":218,"end":493},"obj":"Sentence"},{"id":"S3","span":{"begin":494,"end":698},"obj":"Sentence"},{"id":"S4","span":{"begin":699,"end":1029},"obj":"Sentence"},{"id":"S5","span":{"begin":1030,"end":1115},"obj":"Sentence"},{"id":"S6","span":{"begin":1116,"end":1387},"obj":"Sentence"},{"id":"S7","span":{"begin":1388,"end":1536},"obj":"Sentence"}],"text":"Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.\nWe investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma."}

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{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"Phenotype"},{"id":"T2","span":{"begin":315,"end":320},"obj":"Phenotype"},{"id":"T3","span":{"begin":1327,"end":1341},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002664"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0002664"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"HP:0009919"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.\nWe investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"Disease"},{"id":"T2","span":{"begin":315,"end":320},"obj":"Disease"},{"id":"T3","span":{"begin":1327,"end":1341},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005070"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0008380"}],"text":"Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.\nWe investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":18,"end":23},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":358,"end":363},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"}],"text":"Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.\nWe investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":98,"end":107},"obj":"Cell"},{"id":"T3","span":{"begin":399,"end":408},"obj":"Cell"},{"id":"T5","span":{"begin":1186,"end":1195},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000576"},{"id":"A2","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0001054"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000576"},{"id":"A4","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0001054"},{"id":"A5","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0000576"},{"id":"A6","pred":"cl_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL:0001054"}],"text":"Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B, and Sp1 transcription factors.\nWe investigated the molecular basis of the synergistic induction by interferon-gamma (IFN-gamma)/tumor necrosis factor-alpha (TNF-alpha) of human interleukin-6 (IL-6) gene in THP-1 monocytic cells, and compared it with the basis of this induction by lipopolysaccharide (LPS). Functional studies with IL-6 promoter demonstrated that three regions are the targets of the IFN-gamma and/or TNF-alpha action, whereas only one of these regions seemed to be implicated in LPS activation. The three regions concerned are: 1) a region between -73 and -36, which is the minimal element inducible by LPS or TNF-alpha; 2) an element located between -181 and -73, which appeared to regulate the response to IFN-gamma and TNF-alpha negatively; and 3) a distal element upstream of -224, which was inducible by IFN-gamma alone. LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers. Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter. This removal occurred by activation of the constitutive Sp1 factor, whose increased binding activity and phosphorylation were mediated by IFN-gamma."}