PubMed:7298658 JSONTXT

{"project":"Test-Species-PubTator","denotations":[{"id":"2","span":{"begin":25,"end":57},"obj":"Chemical"},{"id":"3","span":{"begin":61,"end":66},"obj":"Species"},{"id":"39","span":{"begin":151,"end":156},"obj":"Species"},{"id":"40","span":{"begin":202,"end":207},"obj":"Chemical"},{"id":"41","span":{"begin":226,"end":265},"obj":"Chemical"},{"id":"42","span":{"begin":271,"end":306},"obj":"Chemical"},{"id":"43","span":{"begin":309,"end":341},"obj":"Chemical"},{"id":"44","span":{"begin":348,"end":390},"obj":"Chemical"},{"id":"45","span":{"begin":392,"end":416},"obj":"Chemical"},{"id":"46","span":{"begin":422,"end":469},"obj":"Chemical"},{"id":"47","span":{"begin":471,"end":481},"obj":"Chemical"},{"id":"48","span":{"begin":652,"end":676},"obj":"Chemical"},{"id":"49","span":{"begin":700,"end":710},"obj":"Chemical"},{"id":"50","span":{"begin":828,"end":833},"obj":"Chemical"},{"id":"51","span":{"begin":850,"end":874},"obj":"Chemical"},{"id":"52","span":{"begin":879,"end":902},"obj":"Chemical"},{"id":"53","span":{"begin":904,"end":907},"obj":"Chemical"},{"id":"54","span":{"begin":916,"end":921},"obj":"Chemical"},{"id":"55","span":{"begin":940,"end":968},"obj":"Chemical"},{"id":"56","span":{"begin":974,"end":976},"obj":"Chemical"},{"id":"57","span":{"begin":985,"end":990},"obj":"Chemical"},{"id":"58","span":{"begin":1009,"end":1037},"obj":"Chemical"},{"id":"59","span":{"begin":1107,"end":1117},"obj":"Chemical"},{"id":"60","span":{"begin":1123,"end":1128},"obj":"Chemical"},{"id":"61","span":{"begin":1206,"end":1212},"obj":"Chemical"},{"id":"62","span":{"begin":1273,"end":1276},"obj":"Chemical"},{"id":"63","span":{"begin":1277,"end":1279},"obj":"Chemical"},{"id":"64","span":{"begin":1322,"end":1327},"obj":"Chemical"},{"id":"65","span":{"begin":1329,"end":1371},"obj":"Chemical"},{"id":"66","span":{"begin":1408,"end":1413},"obj":"Chemical"},{"id":"67","span":{"begin":1533,"end":1551},"obj":"Chemical"},{"id":"68","span":{"begin":1556,"end":1579},"obj":"Chemical"},{"id":"69","span":{"begin":1648,"end":1650},"obj":"Chemical"},{"id":"70","span":{"begin":1659,"end":1664},"obj":"Chemical"},{"id":"71","span":{"begin":1687,"end":1710},"obj":"Chemical"},{"id":"72","span":{"begin":1724,"end":1730},"obj":"Chemical"},{"id":"73","span":{"begin":1740,"end":1749},"obj":"Chemical"}],"attributes":[{"id":"A2","pred":"resolved_to","subj":"2","obj":"-"},{"id":"A3","pred":"resolved_to","subj":"3","obj":"10090"},{"id":"A39","pred":"resolved_to","subj":"39","obj":"10090"},{"id":"A40","pred":"resolved_to","subj":"40","obj":"MESH:D008055"},{"id":"A41","pred":"resolved_to","subj":"41","obj":"-"},{"id":"A42","pred":"resolved_to","subj":"42","obj":"-"},{"id":"A43","pred":"resolved_to","subj":"43","obj":"-"},{"id":"A44","pred":"resolved_to","subj":"44","obj":"-"},{"id":"A45","pred":"resolved_to","subj":"45","obj":"MESH:C475298"},{"id":"A46","pred":"resolved_to","subj":"46","obj":"MESH:C561601"},{"id":"A47","pred":"resolved_to","subj":"47","obj":"MESH:C561601"},{"id":"A48","pred":"resolved_to","subj":"48","obj":"MESH:C475298"},{"id":"A49","pred":"resolved_to","subj":"49","obj":"MESH:C561601"},{"id":"A50","pred":"resolved_to","subj":"50","obj":"MESH:D008055"},{"id":"A51","pred":"resolved_to","subj":"51","obj":"MESH:C475298"},{"id":"A52","pred":"resolved_to","subj":"52","obj":"MESH:D019158"},{"id":"A53","pred":"resolved_to","subj":"53","obj":"MESH:C000615234"},{"id":"A54","pred":"resolved_to","subj":"54","obj":"MESH:D008055"},{"id":"A55","pred":"resolved_to","subj":"55","obj":"-"},{"id":"A56","pred":"resolved_to","subj":"56","obj":"MESH:D014316"},{"id":"A57","pred":"resolved_to","subj":"57","obj":"MESH:D008055"},{"id":"A58","pred":"resolved_to","subj":"58","obj":"-"},{"id":"A59","pred":"resolved_to","subj":"59","obj":"MESH:D058428"},{"id":"A60","pred":"resolved_to","subj":"60","obj":"MESH:D014867"},{"id":"A61","pred":"resolved_to","subj":"61","obj":"MESH:D008055"},{"id":"A62","pred":"resolved_to","subj":"62","obj":"MESH:C000615234"},{"id":"A63","pred":"resolved_to","subj":"63","obj":"MESH:D014316"},{"id":"A64","pred":"resolved_to","subj":"64","obj":"MESH:D008055"},{"id":"A65","pred":"resolved_to","subj":"65","obj":"-"},{"id":"A66","pred":"resolved_to","subj":"66","obj":"MESH:D008055"},{"id":"A67","pred":"resolved_to","subj":"67","obj":"-"},{"id":"A68","pred":"resolved_to","subj":"68","obj":"MESH:D019158"},{"id":"A69","pred":"resolved_to","subj":"69","obj":"MESH:D014316"},{"id":"A70","pred":"resolved_to","subj":"70","obj":"MESH:D008055"},{"id":"A71","pred":"resolved_to","subj":"71","obj":"MESH:D019158"},{"id":"A72","pred":"resolved_to","subj":"72","obj":"MESH:D002244"},{"id":"A73","pred":"resolved_to","subj":"73","obj":"MESH:D005690"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"Test-Species-PubDictionaries","denotations":[{"id":"T1","span":{"begin":61,"end":66},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":151,"end":156},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10090"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10088"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10090"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"Test-Species-PubDictionaries-PubMedBERT","denotations":[{"id":"T1","span":{"begin":61,"end":66},"obj":"Species"},{"id":"T3","span":{"begin":151,"end":156},"obj":"Species"},{"id":"T5","span":{"begin":260,"end":265},"obj":"Species"},{"id":"T6","span":{"begin":684,"end":690},"obj":"Species"},{"id":"T7","span":{"begin":716,"end":722},"obj":"Species"},{"id":"T8","span":{"begin":1227,"end":1231},"obj":"Species"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10090"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10088"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10090"},{"id":"A5","pred":"db_id","subj":"T5","obj":"2044967"},{"id":"A6","pred":"db_id","subj":"T6","obj":"13150"},{"id":"A7","pred":"db_id","subj":"T7","obj":"13150"},{"id":"A8","pred":"db_id","subj":"T8","obj":"582003"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":446,"end":469},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":879,"end":902},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":945,"end":968},"obj":"Glycan_Motif"},{"id":"T7","span":{"begin":1556,"end":1579},"obj":"Glycan_Motif"},{"id":"T9","span":{"begin":1687,"end":1710},"obj":"Glycan_Motif"},{"id":"T11","span":{"begin":1740,"end":1749},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A2","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A4","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A6","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI"},{"id":"A7","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A8","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI"},{"id":"A9","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A10","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G50850NI"},{"id":"A11","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G68158BT"},{"id":"A12","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G65889KE"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":84},"obj":"Sentence"},{"id":"T2","span":{"begin":85,"end":483},"obj":"Sentence"},{"id":"T3","span":{"begin":484,"end":617},"obj":"Sentence"},{"id":"T4","span":{"begin":618,"end":723},"obj":"Sentence"},{"id":"T5","span":{"begin":724,"end":766},"obj":"Sentence"},{"id":"T6","span":{"begin":767,"end":903},"obj":"Sentence"},{"id":"T7","span":{"begin":904,"end":1118},"obj":"Sentence"},{"id":"T8","span":{"begin":1119,"end":1262},"obj":"Sentence"},{"id":"T9","span":{"begin":1263,"end":1372},"obj":"Sentence"},{"id":"T10","span":{"begin":1373,"end":1580},"obj":"Sentence"},{"id":"T11","span":{"begin":1581,"end":1750},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":84},"obj":"Sentence"},{"id":"T2","span":{"begin":85,"end":483},"obj":"Sentence"},{"id":"T3","span":{"begin":484,"end":617},"obj":"Sentence"},{"id":"T4","span":{"begin":618,"end":723},"obj":"Sentence"},{"id":"T5","span":{"begin":724,"end":766},"obj":"Sentence"},{"id":"T6","span":{"begin":767,"end":903},"obj":"Sentence"},{"id":"T7","span":{"begin":904,"end":1118},"obj":"Sentence"},{"id":"T8","span":{"begin":1119,"end":1262},"obj":"Sentence"},{"id":"T9","span":{"begin":1263,"end":1372},"obj":"Sentence"},{"id":"T10","span":{"begin":1373,"end":1580},"obj":"Sentence"},{"id":"T11","span":{"begin":1581,"end":1750},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"http://purl.obolibrary.org/obo/MAT_0000098"},{"id":"T2","span":{"begin":157,"end":162},"obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":446,"end":469},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T2","span":{"begin":446,"end":469},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T3","span":{"begin":879,"end":902},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T4","span":{"begin":879,"end":902},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T5","span":{"begin":945,"end":968},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T6","span":{"begin":945,"end":968},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T7","span":{"begin":1556,"end":1579},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T8","span":{"begin":1556,"end":1579},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T9","span":{"begin":1687,"end":1710},"obj":"https://glytoucan.org/Structures/Glycans/G50850NI"},{"id":"T10","span":{"begin":1687,"end":1710},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T11","span":{"begin":1740,"end":1749},"obj":"https://glytoucan.org/Structures/Glycans/G65889KE"},{"id":"T12","span":{"begin":1740,"end":1749},"obj":"https://glytoucan.org/Structures/Glycans/G68158BT"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"Body_part"},{"id":"T2","span":{"begin":157,"end":162},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000098"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":475,"end":481},"obj":"Glycan"},{"id":"T2","span":{"begin":704,"end":710},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A3","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A4","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":475,"end":481},"obj":"Glycan"},{"id":"T2","span":{"begin":704,"end":710},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A3","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G76685HR"},{"id":"A4","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"Body_part"},{"id":"T2","span":{"begin":157,"end":162},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos15-Taxon","denotations":[{"id":"T1","span":{"begin":61,"end":66},"obj":"Organism"},{"id":"T3","span":{"begin":151,"end":156},"obj":"Organism"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10090"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10088"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10090"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":84},"obj":"Sentence"},{"id":"T2","span":{"begin":85,"end":483},"obj":"Sentence"},{"id":"T3","span":{"begin":484,"end":617},"obj":"Sentence"},{"id":"T4","span":{"begin":618,"end":723},"obj":"Sentence"},{"id":"T5","span":{"begin":724,"end":766},"obj":"Sentence"},{"id":"T6","span":{"begin":767,"end":903},"obj":"Sentence"},{"id":"T7","span":{"begin":904,"end":1118},"obj":"Sentence"},{"id":"T8","span":{"begin":1119,"end":1262},"obj":"Sentence"},{"id":"T9","span":{"begin":1263,"end":1372},"obj":"Sentence"},{"id":"T10","span":{"begin":1373,"end":1580},"obj":"Sentence"},{"id":"T11","span":{"begin":1581,"end":1750},"obj":"Sentence"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos15-FMA","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"Body_part"},{"id":"T2","span":{"begin":73,"end":83},"obj":"Body_part"},{"id":"T3","span":{"begin":128,"end":138},"obj":"Body_part"},{"id":"T4","span":{"begin":157,"end":162},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"FMA:50801"},{"id":"A2","pred":"db_id","subj":"T2","obj":"FMA:67438"},{"id":"A3","pred":"db_id","subj":"T3","obj":"FMA:67438"},{"id":"A4","pred":"db_id","subj":"T4","obj":"FMA:50801"}],"namespaces":[{"prefix":"FMA","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"Body_part"},{"id":"T2","span":{"begin":157,"end":162},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000098"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000098"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":61,"end":66},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":151,"end":156},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10090"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10088"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10090"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":67,"end":72},"obj":"Body_part"},{"id":"T3","span":{"begin":157,"end":162},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000955"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_6110636"}],"text":"Biosynthesis in vitro of sialosylgalactosyldiacylglycerol by mouse brain microsomes.\nA sialyltransferase enzyme, present in the microsomal fraction of mouse brain, catalyzes the synthesis in vitro of a lipid, characterized as 1,2-diacyl-3-beta-D-galactosyl (3 comes from 2 N-acetylneuraminosyl)-sn-glycerol, (sialosylgalactosyldiacylglycerol) from 1,2-diacyl-3-beta-D-galactosyl-sn-glycerol (galactosyldiacylglycerol) and cytidine-5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). The enzymatic activity increases proportionally, over a given range, with increasing concentrations of both substrates and of enzyme. The apparent Km of the enzyme for galactosyldiacylglycerol is 130 microM, and for CMP-NeuNAc, 780 microM. The reaction proceeds optimally at pH 6.2. The product of the enzymatic reaction was characterized as a lipid which contained galactosyldiacylglycerol and N-acetylneuraminic acid. 14C-labeled lipid, synthesized from [14C]N-acetylneuraminic acid, and 3H-labeled lipid, synthesized from [3H]galactosyldiacylglycerol, ran with identical RF values when chromatographed on thin layers of silica gel. The water-soluble products, obtained by mild alkaline deacylation of these two labeled lipids, migrated the same when electrophoresed on paper. The ratio 14C/3H was calculated as 0.83 for doubly labeled lipid, [14C]sialosyl-[3H]galactosyldiacylglycerol. Degradation of this doubly labeled lipid by mild alkaline deacylation, followed by mild acid hydrolysis, yielded products that cochromatographed with standards galactosylglycerol and N-acetylneuraminic acid. Analysis of the products resulting from periodate oxidation of the 3H-labeled lipid demonstrated that the N-acetylneuraminic acid is linked to carbon 3 of the galactose."}