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PubMed:6425294 JSONTXT

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GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 41-62 Glycan_Motif denotes N-acetylgalactosamine https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G27025MB
T2 64-85 Glycan_Motif denotes globotriaosylceramide https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T3 160-169 Glycan_Motif denotes P antigen https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00061MO
T4 334-344 Glycan_Motif denotes Pk antigen https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T5 350-359 Glycan_Motif denotes P antigen https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00061MO
T6 524-545 Glycan_Motif denotes globotriaosylceramide https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T7 922-943 Glycan_Motif denotes globotriaosylceramide https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00059MO
T8 1361-1380 Glycan_Motif denotes Forssman glycolipid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G80062GG|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00064MO

sentences

Id Subject Object Predicate Lexical cue
T1 0-190 Sentence denotes Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase, a synthase of human blood group P antigen, from canine spleen.
T2 191-561 Sentence denotes A UDP-N-acetylgalactosamine:globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase which catalyzes the conversion of human blood group Pk antigen into P antigen has been purified over 18,000-fold in 4% yield from a Triton X-100 extract of canine spleen microsomes by affinity chromatography on UDP-hexanolamine-Sepharose and globotriaosylceramide acid-Sepharose.
T3 562-724 Sentence denotes The purified enzyme migrates as two major bands with apparent molecular weights of 64,000 and 57,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T4 725-832 Sentence denotes A single band, with enzyme activity, was observed in nondenaturing acrylamide gels containing Triton X-100.
T5 833-892 Sentence denotes Mn2+ was required for activity, and the pH optimum was 6.9.
T6 893-981 Sentence denotes Km values for UDP-GalNAc and globotriaosylceramide were 14 and 2.5 microM, respectively.
T7 982-1180 Sentence denotes Studies on substrate specificities indicate that the preferred substrates have the general structure Gal alpha 1-4Gal-OR in which the nature of the R moiety has relatively little effect on activity.
T8 1181-1381 Sentence denotes An antibody against the purified enzyme eliminated the activity of the enzyme, but did not neutralize the alpha-3-N-acetylgalactosaminyltransferase involved in the biosynthesis of Forssman glycolipid.
T1 0-190 Sentence denotes Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase, a synthase of human blood group P antigen, from canine spleen.
T2 191-561 Sentence denotes A UDP-N-acetylgalactosamine:globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase which catalyzes the conversion of human blood group Pk antigen into P antigen has been purified over 18,000-fold in 4% yield from a Triton X-100 extract of canine spleen microsomes by affinity chromatography on UDP-hexanolamine-Sepharose and globotriaosylceramide acid-Sepharose.
T3 562-724 Sentence denotes The purified enzyme migrates as two major bands with apparent molecular weights of 64,000 and 57,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
T4 725-832 Sentence denotes A single band, with enzyme activity, was observed in nondenaturing acrylamide gels containing Triton X-100.
T5 833-892 Sentence denotes Mn2+ was required for activity, and the pH optimum was 6.9.
T6 893-981 Sentence denotes Km values for UDP-GalNAc and globotriaosylceramide were 14 and 2.5 microM, respectively.
T7 982-1180 Sentence denotes Studies on substrate specificities indicate that the preferred substrates have the general structure Gal alpha 1-4Gal-OR in which the nature of the R moiety has relatively little effect on activity.
T8 1181-1381 Sentence denotes An antibody against the purified enzyme eliminated the activity of the enzyme, but did not neutralize the alpha-3-N-acetylgalactosaminyltransferase involved in the biosynthesis of Forssman glycolipid.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 41-62 https://glytoucan.org/Structures/Glycans/G27025MB denotes N-acetylgalactosamine
T2 64-85 https://glytoucan.org/Structures/Glycans/G00059MO denotes globotriaosylceramide
T3 160-169 https://glytoucan.org/Structures/Glycans/G00061MO denotes P antigen
T4 334-344 https://glytoucan.org/Structures/Glycans/G00059MO denotes Pk antigen
T5 350-359 https://glytoucan.org/Structures/Glycans/G00061MO denotes P antigen
T6 524-545 https://glytoucan.org/Structures/Glycans/G00059MO denotes globotriaosylceramide
T7 922-943 https://glytoucan.org/Structures/Glycans/G00059MO denotes globotriaosylceramide
T8 1361-1380 https://glytoucan.org/Structures/Glycans/G00064MO denotes Forssman glycolipid
T9 1361-1380 https://glytoucan.org/Structures/Glycans/G80062GG denotes Forssman glycolipid

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 334-344 http://www.glycoepitope.jp/epitopes/EP0045 denotes Pk antigen
T2 334-344 http://www.glycoepitope.jp/epitopes/AN0211 denotes Pk antigen

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 148-153 http://purl.obolibrary.org/obo/MAT_0000083 denotes blood
T2 148-153 http://purl.obolibrary.org/obo/MAT_0000315 denotes blood
T3 183-189 http://purl.obolibrary.org/obo/MAT_0000085 denotes spleen
T4 322-327 http://purl.obolibrary.org/obo/MAT_0000083 denotes blood
T5 322-327 http://purl.obolibrary.org/obo/MAT_0000315 denotes blood
T6 445-451 http://purl.obolibrary.org/obo/MAT_0000085 denotes spleen

glycogenes

Id Subject Object Predicate Lexical cue
PD-GlycoGenes20190927-B_T1 360-363 https://acgg.asia/db/ggdb/info/gg135 denotes has
PD-GlycoGenes20190927-B_T2 1139-1142 https://acgg.asia/db/ggdb/info/gg135 denotes has

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 148-153 Body_part denotes blood http://purl.obolibrary.org/obo/MAT_0000083|http://purl.obolibrary.org/obo/MAT_0000315
T3 183-189 Body_part denotes spleen http://purl.obolibrary.org/obo/MAT_0000085
T4 322-327 Body_part denotes blood http://purl.obolibrary.org/obo/MAT_0000083|http://purl.obolibrary.org/obo/MAT_0000315
T6 445-451 Body_part denotes spleen http://purl.obolibrary.org/obo/MAT_0000085

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 142-147 OrganismTaxon denotes human 9606
T2 316-321 OrganismTaxon denotes human 9606

Glycosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 334-344 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes Pk antigen http://www.glycoepitope.jp/epitopes/EP0045|http://www.glycoepitope.jp/epitopes/AN0211

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 148-153 Body_part denotes blood http://purl.obolibrary.org/obo/UBERON_0000178
T2 176-182 Body_part denotes canine http://purl.obolibrary.org/obo/UBERON_0003674
T3 183-189 Body_part denotes spleen http://purl.obolibrary.org/obo/UBERON_0002106
T4 322-327 Body_part denotes blood http://purl.obolibrary.org/obo/UBERON_0000178
T5 438-444 Body_part denotes canine http://purl.obolibrary.org/obo/UBERON_0003674
T6 445-451 Body_part denotes spleen http://purl.obolibrary.org/obo/UBERON_0002106

Glycosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 734-738 Cell denotes band http://purl.obolibrary.org/obo/CL:0000560