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PubMed:6290487 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-124 Sentence denotes Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter.
T2 125-127 Sentence denotes I.
T3 128-165 Sentence denotes Cloning and characterization studies.
T4 166-338 Sentence denotes To assess the role of neighboring DNA sequences in gene regulation, poly(dA).poly(dT) and poly(dG).poly(dC) were cloned adjacent to promoters of the lactose control region.
T5 339-680 Sentence denotes Recombinant plasmids were constructed which were suitable for large scale purification of restriction fragments containing these promoters, 95-base pair (bp) AluI fragments containing the lack operator and promoter for the lac wild type and for the catabolite gene activating the protein-independent mutant, lac UV5, were cloned into pBR322.
T6 681-799 Sentence denotes Homopolymers of varying lengths were inserted into the -60 region of these promoters using recombinant DNA techniques.
T7 800-1084 Sentence denotes Six of the recombinant plasmids were chosen for detailed analysis: wild type (wt); wt-AT, containing 70 bp of poly(dA).poly(dT); wt-GC, containing 23 bp of poly(dG).poly(dC); UV5; UV5-AT, containing 70 bp of poly(dA).poly(dT) and finally UV5-GC, containing 43 bp of poly(dG).poly(dC).
T8 1085-1161 Sentence denotes These plasmids were characterized by restriction mapping and DNA sequencing.
T9 1162-1324 Sentence denotes The effects of the DNA homopolymers on the interaction of the Escherichia coli RNA polymerase with the promoters were studied using nitrocellulose filter binding.
T10 1325-1463 Sentence denotes The results show that poly(dA).poly(dT) increases the level of RNA polymerase binding, whereas poly(dG).poly(dC) has no detectable effect.
T1 0-124 Sentence denotes Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter.
T2 125-127 Sentence denotes I.
T3 128-165 Sentence denotes Cloning and characterization studies.
T4 166-338 Sentence denotes To assess the role of neighboring DNA sequences in gene regulation, poly(dA).poly(dT) and poly(dG).poly(dC) were cloned adjacent to promoters of the lactose control region.
T5 339-680 Sentence denotes Recombinant plasmids were constructed which were suitable for large scale purification of restriction fragments containing these promoters, 95-base pair (bp) AluI fragments containing the lack operator and promoter for the lac wild type and for the catabolite gene activating the protein-independent mutant, lac UV5, were cloned into pBR322.
T6 681-799 Sentence denotes Homopolymers of varying lengths were inserted into the -60 region of these promoters using recombinant DNA techniques.
T7 800-1084 Sentence denotes Six of the recombinant plasmids were chosen for detailed analysis: wild type (wt); wt-AT, containing 70 bp of poly(dA).poly(dT); wt-GC, containing 23 bp of poly(dG).poly(dC); UV5; UV5-AT, containing 70 bp of poly(dA).poly(dT) and finally UV5-GC, containing 43 bp of poly(dG).poly(dC).
T8 1085-1161 Sentence denotes These plasmids were characterized by restriction mapping and DNA sequencing.
T9 1162-1324 Sentence denotes The effects of the DNA homopolymers on the interaction of the Escherichia coli RNA polymerase with the promoters were studied using nitrocellulose filter binding.
T10 1325-1463 Sentence denotes The results show that poly(dA).poly(dT) increases the level of RNA polymerase binding, whereas poly(dG).poly(dC) has no detectable effect.

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 107-114 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T2 315-322 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 90-106 OrganismTaxon denotes Escherichia coli 562
T2 1224-1240 OrganismTaxon denotes Escherichia coli 562

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 234-238 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T3 243-247 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T5 256-260 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T7 265-269 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T9 910-914 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T11 919-923 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T13 956-960 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T15 965-969 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T17 1008-1012 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T19 1017-1021 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T21 1066-1070 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T23 1075-1079 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T25 1347-1351 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T27 1356-1360 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T29 1420-1424 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T31 1429-1433 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 407-412 Body_part denotes scale http://purl.obolibrary.org/obo/UBERON_0002542

sentences

Id Subject Object Predicate Lexical cue
T1 0-124 Sentence denotes Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter.
T2 125-127 Sentence denotes I.
T3 128-165 Sentence denotes Cloning and characterization studies.
T4 166-338 Sentence denotes To assess the role of neighboring DNA sequences in gene regulation, poly(dA).poly(dT) and poly(dG).poly(dC) were cloned adjacent to promoters of the lactose control region.
T5 339-680 Sentence denotes Recombinant plasmids were constructed which were suitable for large scale purification of restriction fragments containing these promoters, 95-base pair (bp) AluI fragments containing the lack operator and promoter for the lac wild type and for the catabolite gene activating the protein-independent mutant, lac UV5, were cloned into pBR322.
T6 681-799 Sentence denotes Homopolymers of varying lengths were inserted into the -60 region of these promoters using recombinant DNA techniques.
T7 800-1084 Sentence denotes Six of the recombinant plasmids were chosen for detailed analysis: wild type (wt); wt-AT, containing 70 bp of poly(dA).poly(dT); wt-GC, containing 23 bp of poly(dG).poly(dC); UV5; UV5-AT, containing 70 bp of poly(dA).poly(dT) and finally UV5-GC, containing 43 bp of poly(dG).poly(dC).
T8 1085-1161 Sentence denotes These plasmids were characterized by restriction mapping and DNA sequencing.
T9 1162-1324 Sentence denotes The effects of the DNA homopolymers on the interaction of the Escherichia coli RNA polymerase with the promoters were studied using nitrocellulose filter binding.
T10 1325-1463 Sentence denotes The results show that poly(dA).poly(dT) increases the level of RNA polymerase binding, whereas poly(dG).poly(dC) has no detectable effect.
T1 0-124 Sentence denotes Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter.
T2 125-127 Sentence denotes I.
T3 128-165 Sentence denotes Cloning and characterization studies.
T4 166-338 Sentence denotes To assess the role of neighboring DNA sequences in gene regulation, poly(dA).poly(dT) and poly(dG).poly(dC) were cloned adjacent to promoters of the lactose control region.
T5 339-680 Sentence denotes Recombinant plasmids were constructed which were suitable for large scale purification of restriction fragments containing these promoters, 95-base pair (bp) AluI fragments containing the lack operator and promoter for the lac wild type and for the catabolite gene activating the protein-independent mutant, lac UV5, were cloned into pBR322.
T6 681-799 Sentence denotes Homopolymers of varying lengths were inserted into the -60 region of these promoters using recombinant DNA techniques.
T7 800-1084 Sentence denotes Six of the recombinant plasmids were chosen for detailed analysis: wild type (wt); wt-AT, containing 70 bp of poly(dA).poly(dT); wt-GC, containing 23 bp of poly(dG).poly(dC); UV5; UV5-AT, containing 70 bp of poly(dA).poly(dT) and finally UV5-GC, containing 43 bp of poly(dG).poly(dC).
T8 1085-1161 Sentence denotes These plasmids were characterized by restriction mapping and DNA sequencing.
T9 1162-1324 Sentence denotes The effects of the DNA homopolymers on the interaction of the Escherichia coli RNA polymerase with the promoters were studied using nitrocellulose filter binding.
T10 1325-1463 Sentence denotes The results show that poly(dA).poly(dT) increases the level of RNA polymerase binding, whereas poly(dG).poly(dC) has no detectable effect.

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-124 Sentence denotes Effects of neighboring DNA homopolymers on the biochemical and physical properties of the Escherichia coli lactose promoter.
T2 125-165 Sentence denotes I. Cloning and characterization studies.
T3 166-338 Sentence denotes To assess the role of neighboring DNA sequences in gene regulation, poly(dA).poly(dT) and poly(dG).poly(dC) were cloned adjacent to promoters of the lactose control region.
T4 339-680 Sentence denotes Recombinant plasmids were constructed which were suitable for large scale purification of restriction fragments containing these promoters, 95-base pair (bp) AluI fragments containing the lack operator and promoter for the lac wild type and for the catabolite gene activating the protein-independent mutant, lac UV5, were cloned into pBR322.
T5 681-799 Sentence denotes Homopolymers of varying lengths were inserted into the -60 region of these promoters using recombinant DNA techniques.
T6 800-1084 Sentence denotes Six of the recombinant plasmids were chosen for detailed analysis: wild type (wt); wt-AT, containing 70 bp of poly(dA).poly(dT); wt-GC, containing 23 bp of poly(dG).poly(dC); UV5; UV5-AT, containing 70 bp of poly(dA).poly(dT) and finally UV5-GC, containing 43 bp of poly(dG).poly(dC).
T7 1085-1161 Sentence denotes These plasmids were characterized by restriction mapping and DNA sequencing.
T8 1162-1324 Sentence denotes The effects of the DNA homopolymers on the interaction of the Escherichia coli RNA polymerase with the promoters were studied using nitrocellulose filter binding.
T9 1325-1463 Sentence denotes The results show that poly(dA).poly(dT) increases the level of RNA polymerase binding, whereas poly(dG).poly(dC) has no detectable effect.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 107-114 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE
T2 315-322 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 90-106 OrganismTaxon denotes Escherichia coli 562
T2 1224-1240 OrganismTaxon denotes Escherichia coli 562

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 407-412 Body_part denotes scale http://purl.obolibrary.org/obo/UBERON_0002542

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 234-238 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T3 243-247 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T5 256-260 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T7 265-269 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T9 910-914 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T11 919-923 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T13 956-960 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T15 965-969 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T17 1008-1012 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T19 1017-1021 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T21 1066-1070 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T23 1075-1079 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T25 1347-1351 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T27 1356-1360 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T29 1420-1424 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775
T31 1429-1433 Cell denotes poly http://purl.obolibrary.org/obo/CL:0000096|http://purl.obolibrary.org/obo/CL:0000775